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Roles of phosphatidylinositol-3-kinases signaling pathway in inflammation-related cancer:Impact of rs10889677 variant and buparlisib in colitis-associated cancer

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World Journal of Gastroenterology 2023年第40期29卷 5543-5556页

作者： Nurul Nadirah Razali raja Affendi raja Ali Khairul Najmi Muhammad Nawawi Azyani Yahaya Norshafila Diana Mohd Rathi Norfilza Mohd Mokhtar Department of Physiology Faculty of MedicineUniversiti Kebangsaan MalaysiaCheras 56000Kuala LumpurMalaysia School of Medical and Life Sciences Sunway UniversitySunway City 47500Malaysia GUT Research Group Faculty of MedicineUniversiti Kebangsaan MalaysiaCheras 56000Kuala LumpurMalaysia Gastroenterology Unit Department of MedicineFaculty of MedicineUniversiti Kebangsaan MalaysiaCheras 56000Kuala LumpurMalaysia Department of Pathology Faculty of MedicineUniversiti Kebangsaan MalaysiaCheras 56000Kuala LumpurMalaysia

BACKGROUND Phosphatidylinositol-3-kinases(PI3K)is a well-known route in inflammationrelated *** discovery on PI3K-related genes revealed a potential variant that links ulcerative colitis(UC)and colorectal cancer(CRC)with colitisassociated cancer(CAC).PI3K/AKT pathway has been recommended as a potential additional therapeutic option for CRC due to its substantial role in modifying cellular *** is a pan-class I PI3K inhibitor previously shown to reduce tumor *** To investigate the regulation of rs10889677 and the role of buparlisib in the PI3K signaling pathway in CAC *** Genomic DNA from 32 colonic samples,including CAC(n=7),UC(n=10)and CRC(n=15),was sequenced for the rs10889677 *** mutant and wildtype fragments were amplified and cloned in the pmirGLO *** luciferase activity of cloned vectors was assessed after transfection into the HT29 cell *** mice were induced by a mixture of a single azoxymethane injection and three cycles of dextran sulphate sodium,then buparlisib was administered after 14 *** excised colon was subjected to immunohistochemistry for Ki67 and Cleaved-caspase-3 markers and quantitative real-time polymerase chain reaction analysis for Pdk1 and *** Luciferase activity decreased by 2.07-fold in the rs10889677 mutant,confirming the hypothesis that the variant disrupted miRNA binding sites,which led to an increase in IL23R expression and the activation of the PI3K signaling ***,CAC-induced mice had a significantly higher disease activity index(P<0.05).Buparlisib treatment significantly decreased mean weight loss in CAC-induced mice(P<0.05),reduced the percentage of proliferating cells by 5%,and increased the number of apoptotic *** treatment also caused a downward trend of Pdk1 expression and significantly decreased Sgk2 *** Our findings suggested that the rs10889677 variant as a critical initiator of the PI3K signaling pathway,an

关键词： Colitis-associated cancer Colorectal cancer Phosphatidylinositol 3-kinase Animal model Luciferases Renilla Phosphatidylinositol 3-kinase inhibitor

Antibacterial potential of three seagrasses against human pathogens

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Asian Pacific Journal of Tropical Medicine 2010年第11期3卷 890-893页

作者： Rengasamy Ragupathi raja Kannan rajasekaran Arumugam Perumal Anantharaman CAS in Marine Biology Faculty of Marine SciencesAnnamalai UniversityParangipettai-608 502TamilnaduIndia

Objective:To evaluate the antibacterial activity of Halophila stipulacea(***), Cymodocea serrulata(***) and Halodule pinifolia(***) against seven human bacterial ***:The antibacterial activities of the extracts on the various test organisms using disc diffusion method and Minimum Inhibitory Concentraction(MIC).Results: Methanol and chloroform extracts of all the three seagrasses were active against all the tested pathogens,whereas the hexane extract of seagrasses was not active against Staphylococcus aureus (***).Antibacterial activity of three seagrass screened,was in the order of *** > ***.> ***:This antibacterial studies can further investigated on seagrasses for purification of bioactive substance and its possible utility in disease control.

关键词： Seagrass Halophila stipulacea Cymodocea serrulata Halodule pinifolia Antibacterial activity MIC

A reconstruction of Turkey’s potential natural vegetation using climate indicators

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Journal of Forestry Research 2019年第6期30卷 2199-2211页

作者： Nussa bah B.raja Olgu Aydin Ihsan Cicek Necla Turkoglu GeoZentrum Nordbayern University Erlangen-Numberg91054 ErlangenGermany Department of Geography Faculty of HumanitiesAnkara University06100 AnkaraTurkey

Turkey,containing three of the world’s biodiversity hotspots,is a hub for genetic ***,the vegetation cover has drastically changed in recent decades as a result of substantial transformations in landuse practices.A map of the potential natural vegetation can be used to represent the biodiversity of a country,and therefore a reference to effectively develop conservation *** multinomial logistic regression is used to simulate the probability of different biomes occurring in the country using elevation,climatological data and natural vegetation data.A correlation test was applied to the climatological data to determine which predictors influence vegetation the *** were temperature,precipitation,relative humidity and *** Ordinary Kriging method was employed to transform the data into the format for the multinomial logistic regression *** model showed that temperature was the most influencing factor with respect to Turkey’s vegetation and distribution follows a similar distribution as the various *** forests are mostly found in the Black Sea region,which is also the wettest region of the *** Marmara region is the only other region where there are broadleaf *** forests and shrublands are mostly located in Central Anatolia due to the region’s low humidity which favours herbaceous *** forests were dominant in the Aegean and Mediterranean regions,attributed to high temperatures.

关键词： Biomes Multinomial logistic regression Statistical modelling Turkey Vegetation

Bio-analysis of Saliva Using Paper Devices and Colorimetric Assays

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Journal of Analysis and Testing 2024年第1期8卷 114-132页

作者： Adhan Narasimhan Harshil Jain Kowshika Muniandy raja Chinnappan Naresh Kumar Mani Microfluidics Sensors and Diagnostics(μSenD)LaboratoryCentre for MicrofluidicsBiomarkersPhotoceutics&Sensors(μBioPS)Department of BiotechnologyManipal Institute of TechnologyManipal Academy of Higher EducationManipalKarnataka 576104India College of Medicine Alfaisal UniversityRiyadh 11533SaudiArabia Tissue/Organ Bioengineering&BioMEMS Lab Organ Transplant Centre of ExcellenceTransplant Research&Innovation DepartmentKing Faisal Specialist Hospital and Research CentreRiyadhSaudi Arabia

In light of the evolving nature of various diseases,time becomes a crucial factor in diagnosis and identifying the underlying causes.A point-of-care device provides a rapid diagnosis of a disease without using complex and advanced instruments,which are costly and difficult to transport.A paper-based device is a relatively frugal solution wherein the paper is used as a substratum in which the reactions are carried *** methods are non-invasive,and the sample collection is relatively *** is one such body fluid in which various biomarkers are present for numerous *** of saliva has attracted more attention recently due to its non-invasiveness and *** the discovery of clinical biomarkers from salivary analysis has the potential to revolutionize the healthcare sector by providing crucial information to monitor the health status of individuals and disease progression which enables personalized *** review provides the limitation of the traditional methods in clinical applications and highlights the significance of saliva as a non-invasive biological fluid that is a source of multiple biomarkers associated with various *** also provides insights into the different paper-based colorimetric microfluidic devices developed against salivary biomarkers in the past *** major challenges in the point-of-care application and the future prospects have been discussed as ***,we also emphasize the importance of this approach in dental disease diagnosis which is least explored and holds potential applications.

关键词： Paper-based assay Salivary analysis Non-invasive Point-of-care Body fluids Biomarkers Qualitative detection

Self-assembled vertically aligned gold nanorod super-lattices for ultra-high sensitive detection of molecules

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Nano Research 2015年第3期8卷 907-919页

作者： Amey Apte Prashant Bhaskar raja Das Smita Chaturvedi PankajPoddar Sulabha Kulkarni Indian Institute of Science Education and Research (IISER) Homi Bhabha Road Pashan Pune - 411008 India Physical and Materials Chemistry Division CSIR-National Chemical Laboratory Pashan Pune - 411008 India Academy of Scientific and Innovative Research (AcSIR) Anusandhan Bhavan 2 Raft Marg New Delhi - 10000 I India

We show that self-assembled vertically aligned gold nanorod （VA-GNRs） superlattices can serve as probes or substrates for ultra-high sensitive detection of various molecules. D-glucose and 2,4,6-trinitrotoluene （TNT） have been chosen as model systems due to their very low Raman cross-sections （5.6× 10-30 ***-1 for D-glucose and 4.9 × 10-31 ***-1 for TNT） to show that the VA-GNR superlattice assembly offers as low as yoctomole sensitivity. Our experiment on mixed samples of bovine serum albumin （BSA） and D-glucose solutions demonstrate sensitivity for the latter, and the possible extension to real samples. Self-assembled superlattices of VA-GNRs were achieved on a silicon wafer by depositing a drop of solvent containing the GNRs and subsequent solvent evaporation in ambient conditions. An additional advantage of the VA-GNR monolayers is their extremely high reproducible morphology accompanied by ultrahigh sensitivity which will be useful in many fields where a very small amount of analyte is available. Moreover the assembly can be reused a number of times after removing the already present molecules. The method of obtaining VA-GNRs is simple, inexpensive and reproducible. With the help of simulations of monolayers and multilayers it has been shown that superlattices can achieve better sensitivity than monolaver assembly of VA-GNRs.

关键词： nanostructures self-assemblies superlattices Raman spectroscopy sensors

Bio-inspired computational heuristics to study models of HIV infection of CD4＋ T-cell

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International Journal of Biomathematics 2018年第2期11卷 83-114页

作者： Muhammad Asif Zahoor raja Kiran Asma Muhammad Saeed Aslam Department of Electrical Engineering COMSATS Institute of Information Technology Attoek Campus Attock Pakistan Department of Computer Sciences COMSATS Institute of Information Technology Attock Campus Attock Pakistan Pakistan Institute of Engineering and Applied Sciences Nilore Islamabad Pakistan

In this work, biologically-inspired computing framework is developed for HIV infection of CD4＋ T-cell model using feed-forward artificial neural networks （ANNs）, genetic algorithms （GAs）, sequential quadratic programming （SQP） and hybrid approach based on GA-SQP. The mathematical model for HIV infection of CD4＋ T-cells is represented with the help of initial value problems （IVPs） based on the system of ordinary differential equations （ODEs）. The ANN model for the system is constructed by exploiting its strength of universal approximation. An objective function is developed for the system through unsupervised error using ANNs in the mean square sense. Training with weights of ANNs is carried out with GAs for effective global search supported with SQP for efficient local search. The proposed scheme is evaluated on a number of scenarios for the HIV infection model by taking the different levels for infected cells, natural substitution rates of uninfected cells, and virus particles. Comparisons of the approximate solutions are made with results of Adams numerical solver to establish the correctness of the proposed scheme. Accuracy and convergence of the approach are validated through the results of statistical analysis based on the sufficient large number of independent runs.

关键词： HIV infection model bioinformatics artificial neural networks genetic algo-rithms.

Expression and role of nuclear receptor coregulators in colorectal cancer

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World Journal of Gastroenterology 2017年第25期23卷 4480-4490页

作者： Mouna Triki Marion Lapierre Vincent Cavailles raja Mokdad-Gargouri IRCM Institut de Recherche en Cancérologie de Montpellier INSERM U1194 Université de Montpellier Institut régional du Cancer de Montpellier Centre de Biotechnologie de Sfax Université de Sfax

Colorectal cancer(CRC) is one of the most common human cancers and the cause of about 700000 deaths per year worldwide. Deregulation of the WNT/β-catenin pathway is a key event in CRC initiation. This pathway interacts with other nuclear signaling pathways, including members of the nuclear receptor superfamily and their transcription coregulators. In this review, we provide an overview of the literature dealing with the main coactivators(NCo A-1 to 3, NCo A-6, PGC1-α, p300, CREBBP and MED1) and corepressors(N-Co R1 and 2, NRIP1 and MTA1) of nuclear receptors and summarize their links with the WNT/β-catenin signaling cascade, their expression in CRC and their role in intestinal physiopathology.

关键词： Immunohistochemistry Colon cancer Nuclear receptors Transcription coregulators WNT signaling

Trends of dengue infections in Malaysia,2000-2010

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Asian Pacific Journal of Tropical Medicine 2013年第6期6卷 462-466页

作者： Md.Shahin Mia Rawshan Ara Begum A.C.Er raja Datuk Zaharaton raja Zainal Abidin Joy Jacqueline Pereira Institute for Environment and Development Universiti Kebangsaan Malaysia43600UKMBangiSelangor D.E.Malaysia Southeast Asia Disaster Prevention Research Institute Universiti Kebangsaan Malaysia43600UKMBangiSelangor D.E.Malaysia School of Development and Environmental Studies Faculty of Social Science and Humanities Universiti Kebangsaan Malaysia43600UKMBangiSelangor D.E.Malaysia

Objective:To analyze trends of dengue incidences and deaths in Malaysia from 2000 to 2010 as well as the predominant dengue virus serotypes during the last ***:We used the national data on annual reported cases,deaths,incidence rate,mortality rate,and case fatality rate of dengue fever(DF) and dengue hemorrhagic fever(DHF) as well as dengue virus serotypes prevalent in Malaysia during the last ***/ regression lines were fitted to investigate the trend of dengue incidences and deaths due to the disease for a 11-year period(2000-2010).For the distribution of national incidence rate,mortality rate,and case fatality rate of DF and DHF, descriptive statistics using mean and 95%confidence intervals(C/) for means,and range were ***:The number of dengue cases and number of deaths have increased,on average, by 14%and 8%per year *** average annual incidence rate of DF per 100 000 populations was higher as compared to that of ***,the yearly mean mortality rate of DHF per 100 000 populations was greater than that of *** simultaneous circulation of all four dengue serotypes has been found in *** a particular dengue virus serotype predominates for at least two years before it becomes replaced by another ***: The dengue situation in Malaysia has worsened with an increasing number of reported oases and deaths during the last *** increasing trend of dengue highlights the need for a more systematic surveillance and reporting of the disease.

关键词： Dengue Dengue hemorrhagic fever Trend Malaysia

Effect of germicidal UV-C light(254 nm)on eggs and adult of house dustmites,Dermatophagoides pteronyssinus and Dermatophagoides farinae(Astigmata:Pyroglyhidae)

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Asian Pacific Journal of Tropical Biomedicine 2012年第9期2卷 679-683页

作者： Ernieenor Faraliana Che Lah raja Noor Azreen raja Musa Ho Tze Ming Acarology Unit Infectious Disease Research CentreInstitute for Medical Research

Objective:To examined the immediate and 24 hours post-irradiation germicidal effects of UV-C lamp *** and adults of house dust mites Dermatophagoides pteronyssinus(***)and Dermatophagoides farinae(***).Methods:This study investigated the immediate and24 hours post irradiation mortalities of adult mites exposed to UV-C at different exposure times(5mins,10 mins,15 mins,20 mins,30 mins and 60 mins)and distances(10 cm,25 cm,33 cm,45 cm and 55 cm).Fresh eggs of the 2 dust mites were also irradiated at 10,35 and 55 cm for 0.5,1,2,3,and 5 minutes,and observed daily post-irradiation for up to 7 ***:Highest immediate mortality of 100%occurred with direct irradiation al 10 cm distance from UV-C lamp and for 60mins,for both species of *** post 24 hours mean mortality rates were(58.4±17.4)%for *** and(27.7±9.7)%for *** when irradiated for 1 hour at 55 cm distance under UV-C *** mites were irradiated in the presence of culture media,the highest mortality rates were lower compared to the direct irradiation;at 10 cm distance and 60 mins exposure,the mean mortality was(74.0±6.8)%for *** and(70.3±6.7)%for *** hatchability for both species of mites was also notably reduced by greater than 50%following ***:Ultraviolet C irradiation is lethal to an array of organisms by damaging their nucleic acids(DNA and RNA).This study demonstrates the increasing mite mortalities with increasing exposure times and decreasing distances.

关键词： Physical control UV-irradiation Dermatophagoides farinae Dermatophagoides pteronyssinus Mortality