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Application of Stool-PCR test for diagnosis of Helicobacter pylori infection in children

World Journal of Gastroenterology 2009年第4期15卷 484-488页

作者： Tahereh Falsafi raha favaedi Fatemeh Mahjoub Mehri Najafi Department of Biology Alzahra University Department of Gastroenterology and Pathology Medical Center for Children

AIM： To evaluate the usefulness of stooI-PCR test for diagnosis of Helicobacter pylori （H pylon） infection in pediatric populations. METHODS： Based on endoscopic features （including nodular gastritis, erosive duodenitis and ulcer） and/or a positive rapid urease test （RUT） obtained during endoscopy, 28 children from a group of children admitted to the Children＇s Medical Center of Tehran for persistent upper gastrointestinal problems were selected to compare biopsy-based tests with stool- PCR. Their gastric activity and bacterial density were graded by the updated Sydney system, and their first stool after endoscopy was stored at -70℃. Biopsies were cultured on modified campy-blood agar plates and identified by gram-staining, biochemical tests, and PCR. Two methods of phenol-chloroform and boiling were used for DNA extraction from H pylori isolates. Isolation of DNA from stool was performed using a stool DNA extraction kit （Bioneer Inc, Korea）. PCR was performed using primers for detection of vacA, cagA, and 16srRNA genes in both isolates and stool. RESULTS： Sixteen out of 28 child patients （57%） were classified as H pylori positive by biopsy-based tests, of which 11 （39%） were also positive by stool- PCR. Sensitivity and specificity of stool-PCR was 62.5% and 92.3% respectively. H pylori was observed in histological sections for 10 out of 11 stool-positive patients. Association was observed between higher score of H pylori in histology and positivity of stool- PCR. Also association was observed between the more severe form of gastritis and a positive stool-PCR. CONCLUSION： Association between higher score of H pylori in histology and a positive stool-PCR make it a very useful test for detection of H pylori active infection in children. We also suggest that a simple stool-PCR method can be a useful test for detection of Hpylori virulence genes in stool.

关键词： Helicobacter pylori Non-invasive diagnosis Stool-PCR Histology Score Children Iran

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Jet modification in absence of QGP-medium:the role of multiparton interactions and color reconnection

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Chinese Physics C 2024年第1期48卷 50-60页

作者： Prottoy Das Abhi Modak Debjani Banerjee Rathijit Biswas Supriya Das Sanjay KGhosh Sibaji raha Sidharth Kumar Prasad Department of Physics Bose InstituteKolkata 700091India Quark Matter Research Center Institute of Modern PhysicsChinese Academy of SciencesLanzhou 730000China

Recent studies on high-multiplicity events in small collision systems(proton-proton and proton-lead)have drawn considerable research interest toward the possibility of the formation of partonic medium in such *** of the important consequences of the formation of dense partonic medium is the quenching of high-momentum final-state particles,resulting in several experimental observations such as suppression in nuclear modification factor RAA,modification of jet shape observableρ(r)and jet fragmentation(z^(ch))distributions,*** this work,we studyρ(r)and z^(ch)for inclusive charged-particle jets in proton-proton(pp)collisions at√s=13 TeV using the PYTHIA 8 Monash 2013 Monte Carlo *** show that the color reconnection(CR)and multiparton interaction(MPI)mechanisms in PYTHIA 8 can lead to an increased rate of jet *** also find that the mechanisms of MPI and CR and change in the gluonic contribution in high-multiplicity events result in significant modification ofρ(r)and z^(ch)compared to those in minimum bias events for 10and gluonic contribution with the amount of modification inρ(r):the larger the number of MPIs and/or gluonic contribution,the larger the amount of modification ofρ(r).

关键词： jet modification PYTHIA multiparton interaction color reconnection gluonic contribution high-multiplicity

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Serum Calcium Ionised Rate and Materno-Perinatal Prognosis in Arterial Hypertension in Pregnancy at the Reference General Hospital of Panzi

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Open Journal of Obstetrics and Gynecology 2024年第3期14卷 422-434页

作者： Mushera Aganze Alain raha Maroyi Kenny Kakisingi Mibi De Joseph Musese Nguru Marie Constance Julien Bwama Botalatala Mbozi Andrea Koko Kasengire Euphrasie Imani Erahamoba Pince Olivier Nyakio Department of Gynecology and Obstetrics Panzi General Referral Hospital Bukavu Democratic Republic of the Congo Department of Medicine and Public Health Evangelical University in Africa Bukavu Democratic Republic of the Congo Biopharm Hospital Bukavu Democratic Republic of the Congo International Institute of Tropical Agriculture IITA Olusegun Obasanjo Campus Kalambo Station South Kivu Democratic Republic of the Congo School of Medicine Official University of Bukavu Bukavu Democratic Republic of the Congo

Hypertensive disorders of pregnancy are among the leading causes of severe maternal morbidity and mortality, particularly in developing countries. Hypertensive disorders of pregnancy are among the leading causes of severe maternal morbidity and mortality, particularly in developing countries, maternal hypocalcaemia being a factor favouring the onset of arterial hypertension during pregnancy. The aim was to determine the maternal and perinatal prognosis of patients with hypertensive disorders of pregnancy as a function of serum ionised calcium levels. Material and Methods: A cross-sectional analytical study of 114 patients with arterial hypertension during pregnancy or during pregnancy or in the postpartum period at the HGR/Panzi from 1 January 2021 to 30 June 2022, text was entered using Microsoft Office Word 2010 and the tables were analysed using Excel 2010. The data was analysed using SPSS version 20.0 and Stata 14.0. The associations of the variables were calculated using Pearson's chi-square test, with a significance threshold set at a value of p < 0.05. Study of risk factors, Odds ratios and their confidence intervals were estimated in a univariate analysis. The most determining factors were identified by multivariate analysis using the Forward conditional logistic regression model. Results: The mean gestational age was 34.43 ± 4.327 amenorheas weeks, 46.6% of patients had a vaginal delivery, 66.65% of which were indicated for maternal prognosis, maternal complications were associated with maternal hypocalcaemia in 81, 82% (P = 0.043) and an OR = 3.255 (P = 0.0158) threefold risk that the patient presenting with a complication is likely to be in a state of hypocalcaemia at 95% confidence index, and fetal prognosis was not significantly related to maternal calcaemia. Conclusion: Maternal hypocalcaemia is one of the factors that can influence maternal-foetal complications maternal-fetal complications, early management and prevention of this pathology is patholog

关键词： Hypocalcaemia Arterial Hypertension During Pregnancy and Maternal and Perinatal Prognosis

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Quaternary alluvial fan dynamics of the Jaldhaka basin

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Journal of Mountain Science 2022年第8期19卷 2160-2179页

作者： Adrija raha Mery BISWAS Department of Geography Presidency University86/1 College StreetKolkata-700073West BengalIndia

Across the Himalayan foreland area,alluvial fans respond to fluvial depositional landforms created by active tectonics and are incorporated within hydro-sediment *** tributary of the master stream has its water and sediment to discharge,which assists in initiating several mesolevel fans in response to the active tectonics in Jaldhaka *** the mountain front,active MBT(Main Boundary Thrust),MFT(Main Frontal Thrust)and lineaments(parallel and transverse to Himalaya)have governed the tectonically active Quaternary foreland area where numerous meso-level fans have *** study aims to estimate the tectonic activeness of each meso-fan using the morphotectonic *** tributaries of the Jaldhaka River are permuted to the recent tectonism or neotectonics to form each meso fan with its basin displaying the varying degree of their activeness and aggradation *** methods like technique for order performance by similarity to ideal solution(TOPSIS)and VIKOR(Vlse Kriterijumska Optimizacija Kompromisno Resenje)that specify multicriteria optimization and compromise solution,in Serbian have ranked the Fan 2 as mostly active and Fan 4 is comparatively tectonically *** hydro-sediment characteristics reveal the topographic spatial dynamics of Fan 4 where lobe 3 has elevated from *** XRD analysis specifies the scientific key in response to the dominance of dolomite in the aggraded elevated bed sediments carried by the Rohtikhola *** conceptual fan models of Fan 2and 4 display the fan development phases through four *** has clearly defined the extension of the fan lobes of Fan 2 and *** is summarized that within a common climatic zone and similar neo-tectonic setting,meso-level fans have differential morphotectonic signatures with dynamic aggradation processes.

关键词： Himalaya Tectonic activeness Fan dynamics Hydro sediment interaction

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Algorithmic approaches for optimizing electronic control unit time using multi-rate sampling

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Control Theory and Technology 2018年第3期16卷 173-190页

作者： Rajorshee raha Pallab DASGUPTA Soumyajit DEY Department of Computer Science and Engineering Indian Institute of Technology Kharagpur India

With the emerging migration of automotive and other distributed control platforms from federated to integrated architectures, the need for optimal utilization of ECU （electronic control unit） bandwidth will become a key requirement in the implementation of embedded control features. This paper advocates the partitioning of the operating space of the plant and the use of minimal sampling rates in each partition without compromising the overall quality of control. At the heart of the proposed methodology are our algorithms that enable the choice of the partitions and the sampling rate for each partition. We demonstrate the efficacy of our methods on two case studies, namely an anti-lock braking system and a lane departure warning system. We also study the use of a supervisory controller that controls the switching among sampling rates for a combination of the two features.

关键词： Sampling period adaptive control stability automotive software embedded control system

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Differential alterations of positive and negative regulators of beta catenin enhance endogenous expression and activity of beta catenin in A549 non small cell lung cancer(NSCLC)cells

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Genes & Diseases 2016年第4期3卷 282-288页

作者： Supratim Ghatak Sanghamitra raha Crystallography and Molecular Biology Division Saha Institute of Nuclear Physics1/AF BidhannagarKolkata 700064India Department of Biotechnology and ISERC Visva Bharati UniversitySantiniketan731235India Present address:Centre for Cellular and Molecular Biology Uppal RoadHyderabad 500007India

Beta catenin has been well documented in previous studies to be involved in non small cell lung cancer(NSCLC).Beta catenin abundance and transcriptional activity are significantly regulated by several *** it is well known that Akt and Gsk3 beta are respective positive and negative regulators of beta catenin,however,no single study has so far documented how the expression and activity of both positive as well as negative regulators play favorable role on beta catenin expression and activity in *** this study,we compared expression and activity of beta catenin and its regulators in normal lung cell WI38 and NSCLC cell A549 by western blot,qRT-PCR and luciferase *** observed that beta catenin positive regulators(Akt and Hsp90)and negative regulators(Gsk3 beta and microRNA-214)have differential expression and/or activity in NSCLC cell *** the differentially altered statuses of both the positive and negative regulators rendered cumulative positive effect on beta catenin expression and activity in *** study thus suggests that chemotherapeutic modulations of regulating factors are crucial when abrogation and/or inhibition of key oncogenic proteins are necessary for cancer chemotherapy.

关键词： A549 Beta catenin Gsk3 beta miR-214 Non small cell lung cancer

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Tuned reactive power dispatch through modified differential evolution technique

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Frontiers in Energy 2012年第2期6卷 138-147页

作者： S.BISWAS(raha) N.CHAKRABORTY Department of Power Engineering Jadavpur UniversitySaltlake 2nd CampusKolkata 700098India

This paper explores the capability of modified differential evolution （MDE） technique for solving the reactive power dispatch （RPD） problem. The proposed method is based on the basic differential evolution （DE） technique with a few modifications made into it. DE is one of the strongest optimization techniques though it suffers from the problem of slow convergence while global minima appear. The proposed modifications ate tried to resolve the problem. The RPD problem mainly defines loss minimization with stable voltage profile. To solve the RPD problem, the generator bus voltage, transformer tap setting and shunt capacitor placements are controlled by the MDE approach. In this paper, IEEE 14-bus and IEEE 30-bus systems are chosen for MDE implementation. The applied modification show much improved result in comparison to normal DE technique. Comparative study with other softcomputing technique including DE validates the effectiveness of the proposed method.

关键词： reactive power dispatch (RPD) modified differential evolution (MDE) differential evolution algorithm with localizations around the best vector (DELB)

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Application of artificial intelligence in trauma orthopedics: Limitation and prospects

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World Journal of Clinical Cases 2023年第18期11卷 4231-4240页

作者： Maryam Salimi Joshua A Parry raha Shahrokhi Seyedarad Mosalamiaghili Department of Orthopaedic Surgery Denver Health Medical CenterDenverCO 80215United States Student Research Committee Shiraz University of Medical SciencesShiraz ***Iran

The varieties and capabilities of artificial intelligence and machine learning in orthopedic surgery are extensively *** promising method is neural networks,emphasizing big data and computer-based learning systems to develop a statistical fracture-detecting *** derives patterns and rules from outstanding amounts of data to analyze the probabilities of different outcomes using new sets of similar *** sensitivity and specificity of machine learning in detecting fractures vary from previous *** may be most promising in the diagnosis of less-obvious fractures that are more commonly *** studies are necessary to develop more accurate and effective detection models that can be used clinically.

关键词： Artificial intelligence Machine learning Orthopedics Trauma Neural network

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Effect of axial length and anterior chamber depth on the peripheral refraction profile

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International Journal of Ophthalmology(English edition) 2021年第2期14卷 292-298页

作者： Masoud Khorrami-Nejad raha Moradi Alireza Akbarzadeh Baghban Bahram Khosravi Translational Ophthalmology Research Center Farabi Eye HospitalTehran University of Medical SciencesTehran ***Iran School of Rehabilitation Shahid Beheshti University of Medical SciencesTehran ***Iran Proteomics Research Center Department of BiostatisticsSchool of Allied Medical SciencesShahid Beheshti University of Medical Sciences

AIM:To evaluate the effect of axial length(AL)and anterior chamber depth(ACD)on peripheral refractive profile in myopic patients compared to emmetropic ***:This cross-sectional study was conducted in right eyes of 58 participants of whom 38 were emmetropic and 20 were *** and peripheral refraction were measured at 10°,20°,and 30°eccentricities in nasal and temporal fields using an open-field *** Lenstar LS900 was used to measure ACD and *** participants were divided into three groups of short(24.5 mm)according to AL and three groups of low ACD(3.60 mm)according to ***:The mean age of the participants was 22.26±3.09 y(range 18-30 y).The peripheral mean spherical refractive error showed a hypermetropic shift in myopic and emmetropic groups although this shift was more pronounced in the myopic *** results showed significant changes in the spherical equivalent,J0,and J45 astigmatism in all gazes with an increase in eccentricity(P<0.001).The pattern of refractive error changes was more noticeable in long and short eyes versus normal AL ***,the pattern of peripheral refractive changes was much more prominent in the high ACD group versus the normal ACD group and in the normal ACD group versus the low ACD ***:Peripheral refraction changes are greater in participants with AL values outside the normal range and deeper ACD values compared to participants with normal AL and ACD.

关键词： anterior chamber depth axial length peripheral refraction

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Constriction factor based particle swarm optimization for analyzing tuned reactive power dispatch

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Frontiers in Energy 2013年第2期7卷 174-181页

作者： Syamasree BISWAS(raha) Kamal Krishna MANDAL Niladri CHAKRABORTY Department of Power Engineering Jadavpur University Kolkata700098 India

The reactive power dispatch （RPD） problem is a very critical optimization problem of power system which minimizes the real power loss of the transmission system. While solving the said problem, generator bus voltages and transformer tap settings are kept within a stable operating limit. In connection with the RPD problem, solving reactive power is compensated by incorporating shunt capacitors. The particle swarm optimization （PSO） technique is a swarm intelligence based fast working optimization method which is chosen in this paper as an optimization tool. Additionally, the constriction factor is included with the conventional PSO technique to accelerate the convergence property of the applied optimization tool. In this paper, the RPD problem is solved in the case of the two higher bus systems, i.e., the IEEE 57-bus system and the IEEE ll8-bus system. Furthermore, the result of the present paper is compared with a few optimization technique based results to substantiate the effectiveness of the proposed study.

关键词： real power loss minimization voltage stability constriction factor particle swarm optimization (PSO)

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