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Occurrence of Antimicrobials in River Water Samples from Rural Region of the State of Rio de Janeiro, Brazil

Journal of Environmental Protection 2016年第2期7卷 230-241页

作者： Mychelle Alves Monteiro Bernardete Ferraz Spisso Julia Rodrigues Martins Pastor dos Santos rafaela pinto da costa Rosana Gomes Ferreira Mararlene Ulberg Pereira Talita da Silva Miranda Bárbara Rodrigues Geraldino de Andrade Luiz Antonio d’Avila Chemistry School/Federal University of Rio de Janeiro (EQ/UFRJ) Rio de Janeiro Brazil National Institute for Quality Control in Health/Oswaldo Cruz Foundation (INCQS/FIOCRUZ) Rio de Janeiro Brazil University Centre of Barra Mansa (UBM) Barra Mansa Brazil National of Institute of Cancer (INCA) Rio de Janeiro Brazil

The occurrence of antimicrobials in the aquatic environment and drinking water has raised the question of their impact on the environment and public health. Animal production is one of the most expressive activities of Brazilian agribusiness. In order to ensure the productivity and competitiveness of the sector, the use of drugs for therapeutic and prophylactic purposes is a common practice. Due to the continuous release of antimicrobials into the environment, the aim of this study was to compare the frequency of detection of tetracyclines and sulfonamides in surface water collected from rural areas in Lidice District of Rio Claro, in the State of Rio de Janeiro, Brazil. An investigative study was conducted with 24 river water samples analyzed by high-performance liquid chromatography coupled to tandem mass spectrometry and the aim of this study was to determine residues of sulfonamides and tetracyclines based on the USEPA method 1694. The results indicated the presence of sulfamethoxazole and oxytetracycline concentrations at the ng·L-1 level. The applied method showed overall good performance with recoveries above 57%, method detection limits ≤ 7.17 ng·L-1, method quantification limits ≤ 23.90 ng·L-1 and good linearity.

关键词： Antimicrobial LC-MS/MS Surface Water Sulfonamides Tetracyclines

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Biochar influences the cane fields’microbiota and the development of pre-sprouted sugarcane seedlings

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Waste Disposal and Sustainable Energy 2023年第1期5卷 75-88页

作者： Bruna Cristina pinto Adriana Barboza Alves Osania Emerenciano Ferreira Gustavo Henrique Gravatim costa Magno Andréde Oliveira Augusto Cesar da Silva Bezerra Alan Rodrigues Teixeira Machado Postgraduate Program in Environmental Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38202-436Brazil Department of Agricultural and Biological Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38208-436Brazil Postgraduate Program in Geotechnical Engineering Federal University of Ouro PretoOuro PretoMinas Gerais35400-000Brazil Department of Transport Engineering Federal Center for Technological Education of Minas GeraisBelo HorizonteMinas Gerais30421-169Brazil Department of Exact Sciences State University of Minas GeraisJoão Monlevade UnityJoão MonlevadeMinas Gerais35930-314Brazil

This research investigates the soil conditioning effect of sugar cane fields with biochar produced from sugarcane *** influence on the physicochemical and microbiological conditions of the soil and the agronomic performance of sugarcane seedlings was *** this end,the biochar from sugarcane bagasse was produced by pyrolysis in a double drum oven and mixed with soil at rates of 0,1%,3%,and 5%(in weight).The experiment was installed in a greenhouse with two evaluation periods(30 days and 60 days)and five *** pot received a pre-sprouted cane seedling,and the analysis of colony-forming units(bacteria and fungi),physicochemical characteristics of the soil,and growth and development of sugarcane were performed per *** biochar showed a high fixed carbon content(72%)and a specific surface area estimated by the adsorption of methylene blue of 50 m2 g−***,its structure is porous and contains important nutrients(e.g.,CaO,K2O,and P2O5).In the periods evaluated,the applications of the 3%and 5%(in weight)rates of biochar reduced most of the soil fertility ***,the chemical analyses indicated that the sugarcane field soil collected already possessed high *** microbiota was influenced,but only the application of the 3%(in weight)rate at 60 days after planting showed a significant positive effect on the number of bacteria forming units,with an increase of approximately 385%.On the other hand,no significant positive effect on sugarcane development was ***,biochar application in high-fertility sugarcane plantation soil did not result in gains for sugarcane development in the periods evaluated.

关键词： Soil Biometry Microbiology Double drum oven Biochar

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Intravenous vs intraperitoneal mesenchymal stem cells administration:What is the best route for treating experimental colitis?

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World Journal of Gastroenterology 2014年第48期20卷 18228-18239页

作者： Fabiany da costa Goncalves Natália Schneider Fernanda Otesbelgue pinto Fabíola Schons Meyer Fernanda Visioli Bianca Pfaffenseller Patrícia Luciana da costa Lopez Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima Luíse Meurer Ana Helena Paz Centro de Pesquisa Experimental do Hospital de Clínicas de Porto Alegre Porto Alegre 90035-903Rio Grande do SulBrazil Programa de Pós-Gradua??o:Ciências em Gastroenterologia e Hepatologia Universidade Federal do Rio Grande do SulPorto Alegre 90035-903Rio Grande do SulBrazil Faculdade de Odontologia Universidade Federado Rio Grande do SulPorto Alegre 90035-003Rio Grande doSulBrazil

AIM:To investigate the therapeutic effects of mesenchymal stem cells(MSCs)transplanted intraperitoneally andintravenously in a murine model of ***:MSCs were isolated from C57BL/6 mouse adipose *** cultures were analyzed according to morphology,cellular differentiation potential,and surface molecular *** acute colitis was induced in C57BL/6 mice by oral administration of2%dextran sulfate sodium(DSS)in drinking water ad libitum from days 0 to *** mice were treated with1×106 MSCs via intraperitoneal or intravenous injection on days 2 and *** disease activity index was determined daily based on the following parameters:weight loss,stool consistency and presence of blood in the feces and *** compare morphological and functional differences in tissue regeneration between different MSC injection modalities,mice were euthanized on day 8,and their colons were examined for length,weight,and histopathological *** responses were determined by measuring the levels of different serum cytokines using a CBA Th1/Th2/Th17 *** rates were evaluated by terminal deoxynucleotidyl transferase-mediated d UDPbiotin nick end labeling ***:Intravenous infusion of MSCs was more effective than intraperitoneal treatment(P<0.001)in reducing the clinical and histopathologic severity of colitis,which includes weight loss,diarrhea and *** histological evaluation demonstrated decreased colonic inflammation based on reduced crypt loss and reduced infiltration of inflammatory *** therapeutic effect was most likely mediated by the down-regulation of pro-inflammatory cytokines[interleukin(IL)-6 and tumor necrosis factor(TNF)];and by the up-regulation of anti-inflammatory cytokines(IL-10 and IL-4).Intravenous transplantation alsoinduced high levels of IFN that lead to activation of the immunosuppressive activity of the MSCs,which did not occur with intraperitoneal transplantation(P=0.006).An increase in a

关键词： Ulcerative colitis Dextran sulfate sodium Inflammatory bowel disease Mesenchymal stem cell Cell transplantation Intravenous injection Immunomodulation

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Cash transfer during the COVID-19 pandemic:a multicentre,randomised controlled trial

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Family Medicine and Community Health 2021年第4期9卷 50-56,I0016-I0021页

作者： Navindra Persaud Kevin E Thorpe Michael Bedard Stephen W Hwang Andrew pinto Peter Jüni Bruno R da costa 无 Department of Family and Community Medicine University of TorontoTorontoOntarioCanada MAP Centre for Urban Health Solutions Li Ka Shing Knowledge InstituteSt.Michael’s HospitalUniversity of TorontoTorontoOntarioCanada Department of Family and Community Medicine St Michael’s HospitalTorontoOntarioCanada Institute of Health Policy Managementand EvaluationUniversity of TorontoTorontoOntarioCanada dalla Lana School of Public Health University of TorontoTorontoOntarioCanada Applied Health Research Centre Unity Health TorontoSt Michael's HospitalTorontoOntarioCanada Department of Family Medicine Northern Ontario School of MedicineThunder BayOntarioCanada Department of Medicine St Michael's HospitalUnity Health TorontoTorontoOntarioCanada Department of Medicine University of TorontoTorontoOntarioCanada Institute of Primary Health Care(BIHAM) University of BernTorontoSwitzerland DISTANSE COVID-19(Direct Income SupporT and Advice Negating Spread of Epidemic COVID-19:a randomized controlled trial)study team

Objective To evaluate the effect of a one-time cash transfer of$C1000 in people who are unable to physically distance due to insufficient *** Open-label,multi-centre,randomised superiority *** Seven primary care sites in Ontario,Canada;six urban sites associated with ***’s Hospital in Toronto and one in Manitoulin *** 392 individuals who reported trouble affording basic necessities due to disruptions related to *** After random allocation,participants either received the cash transfer of$C1000(n=196)or physical distancing guidelines alone(n=196).Main outcome measures The primary outcome was the maximum number of symptoms consistent with COVID-19 over 14 *** outcomes were meeting clinical criteria for COVID-19,SARS-CoV-2 presence,number of close contacts,general health and ability to afford basic *** The primary outcome of number of symptoms reported by participants did not differ between groups after 2 weeks(cash transfer,mean 1.6 vs 1.9,ratio of means 0.83;95%CI 0.56 to 1.24).There were no statistically significant effects on secondary outcomes of the meeting COVID-19 clinical criteria(7.9%vs 12.8%;risk difference−0.05;95%CI−0.11 to 0.01),SARS-CoV-2 presence(0.5%vs 0.6%;risk difference 0.0095%CI−0.02 to 0.02),mean number of close contacts(3.5 vs 3.7;rate ratio 1.10;95%CI 0.83 to 1.46),general health very good or excellent(60%vs 63%;risk difference−0.0395%CI−0.14 to 0.08)and ability to make ends meet(52%vs 51%;risk difference 0.0195%CI−0.10 to 0.12).Conclusions A single cash transfer did not reduce the COVID-19 symptoms or improve the ability to afford *** studies are needed to determine whether some groups may benefit from financial supports and to determine if a higher level of support is *** registration number NCT04359264.

关键词： Canada centre random

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Colorimetric indicators of açaíanthocyanin extract in the biodegradable polymer matrix to indicate fresh shrimp

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Food Bioscience 2022年第4期48卷 601-612页

作者： Samiris Cocco Teixeira Taíla Veloso de Oliveira Rafael Resende Assis Silva Alane rafaela costa Ribeiro Paulo Cesar Stringheta Thaís Caroline Buttow Rigolon Marcos Roberto Moacir Ribeiro pinto Nilda de Fatima Ferreira Soares Laboratory of Food Packaging Department of Food TechnologyFederal University of ViçosaViçosaMG36570-900Brazil Department of Materials Science and Engineering Federal University of S˜ao CarlosS˜ao CarlosSP13565-905Brazil Laboratory of Pigments and Bioactive Compounds Federal University of ViçosaViçosaMG36570-900Brazil Federal Institute of Espírito Santo Venda Nova do ImigranteES29375000Brazil

Colorimetric indicator or freshness indicators hold great potential to reduce food *** off açaíanthocyanins extract(AE)with different concentrations molars of two plasticizers glycerol(GLY)or triethyl citrate(TEC),for application fresh shrimp,real time were *** AE undergoes a visible pink/violet to green/yellowish color change in response to different pH’s,which is an indicator of microbial spoilage in *** CI were exposed to ammonia,and analyzed through infrared spectroscopy(FT-IR),scanning electron microscopy(SEM),ultraviolet spectroscopy,and visual *** immobilization of the plasticizers into the CA polymer matrices were confirmed through FT-IR spectra and SEM *** vitro chromatic transition of the indicators exposed to ammonia vapors,were visually more perceptible when they were produced with 7.2 mols of GLY or 1.8,3.6,and 7.2 mols of *** the real-time analysis of the application of colorimetric indicators in gray shrimp samples,the indicators that had plasticizers showed perfect color change at the exact time of food *** colorimetric indicators developed in this study using different molar concentrations of plasticizers are simple,effective and a great eco-friendly alternative to chemical sensors already developed so far.

关键词： Cellulose acetate Plasticizer Ammonia sensitivity Glycerol Triethyl citrate Spoilage Fish

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SARS-CoV-2 and hearing:An audiometric analysis of COVID-19 hospitalized patients

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Journal of Otology 2021年第3期16卷 158-164页

作者： Francisco Alves de Sousa Rodrigo pinto costa Sandra Xará Ana Nóbrega pinto Cecília Almeida e Sousa Centro Hospitalar Universitario do Porto Serviço de Otorrinolaringologia e Cirurgia da Cabeça e Pescoço(Otorhinolaryngology and Head&Neck surgery Department)Largo Prof.Abel Salazar4099-001PortoPortugal Unidade de Saúde Familiar Garcia de Orta ACeS Porto OcidentalMedicina Geral e Familiar(Family Medicine)R.Pinho Leal 294150-620PortoPortugal Centro Hospitalar Universitario do Porto Serviço de Doenças Infeciosas(Infectious Diseases Department)Largo Prof.Abel Salazar4099-001PortoPortugal

Purpose COVID-19 associated hearing loss is still an ongoing matter of *** original studies exist on audiological effects of SARS-CoV-2 infection in hospitalized *** main objective was to determine whether SARS-CoV-2 may affect auditory function in clinically ill COVID-19 *** and methods COVID-19 patients with moderate-severe disease and without prior history of hearing abnormalities were enrolled from a tertiary referral center,and matched with *** performed an audiometric evaluation,and thresholds were *** 120 ears from 60 patients were *** with COVID-19 showed worse mean auditory thresholds starting from 1000 Hz through higher frequencies,when compared to controls(1000 Hz:18.52±5.49 dB HL in controls vs 25.36±6.79 dB HL in COVID-19,p<0.001;2000Hz:17.50±5.57 dB HL in controls vs 21.96±7.05 dB HL in COVID-19,p=0.010;3000Hz:17.97±8.07 dB HL in controls vs 25±9.38 dB HL in COVID-19,p=0.003;4000 Hz:20.16±10.12 dB HL in controls vs 29.55±11.26 dB HL in COVID-19,p=0.001;8000 Hz:31.09±12.75 dB HL in controls vs 40.71±19.40 dB HL in COVID-19,p=0.030;Pure Tone Average:20.42±4.29 dB HL in controls vs 24.85±5.62 dB HL in COVID-19,p=0.001).Statistical significance persisted after adjusting for confounders such as age,gender and various comorbidities(p<0.05).Conclusions SARS-CoV-2 may affect hearing in COVID-19 patients with moderate-severe *** are in line with the previous suggested effects of COVID-19 on auditory *** study is expected to encourage further research on this topic.

关键词： SARS-CoV-2 COVID-19 Coronavirus Audiometry Hearing

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Valorization of the agro-industrial by-products of bacupari(Garcinia brasiliensis(Mart.))through production of flour with bioactive properties

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Food Bioscience 2022年第1期45卷 377-385页

作者： Anely Maciel de Melo rafaela Cristina Turola Barbi Bruno Patrício costa Mˆonica Ikeda danielle Carpine Rosemary Hoffmann Ribani Food Engineering Graduate Program Federal University of ParanaDepartment of Chemical Engineering81531-990CuritibaPRBrazil

Brazil has a wide variety of fruits that remain insufficiently *** encourage the use of one such fruit,the bacupari((Garcinia brasiliensis(Mart.)),the current study produced flours from its by-products(the peel and seed)at two maturation stages and subsequently evaluated their proximate composition,physical,antioxidant,bioactive,microstructural and thermal *** results revealed that the flours have a high in fiber content(>15%)and are a potential source of protein(>5%).Seed flour has an energy value similar to that of wheat flour and other conventional *** flour micrographs indicate the presence of starch,a fact confirmed by viscosity *** flours contain phenolic compounds and exhibit antioxidant activity,especially the unripe peel *** the compounds identified by chromatography,those present in the greatest concentrations were quinine(≅111μg/g),p-coumaric acid(≅25μg/g),m-coumaric acid(≅15μg/g),and formononetin(≅21μg/g),all of which are known to have several human health *** flour crystallinity diminished as the fruit *** flour exhibited similar thermal transitions but less thermal stability than seed *** summary,this study revealed that flours produced from the peel and seeds of bacupari,which are generated in large quantities as agro-industrial by-products,can be used as alternative ingredients in various sectors of the food industry.

关键词： Antioxidant activity Bacupari fruit Seed Peel Phenolic compounds

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Competition among rare earth elements on sorption onto six seaweeds

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Journal of Rare Earths 2021年第6期39卷 734-741,I0005页

作者： Joāo pinto Marcelo costa Bruno Henriques JoséSoares Mariana Dias Thainara Viana Nicole Ferreira Carlos Vale JoséPinheiro-Torres Eduarda Pereira Department of Chemistry University of Aveiro3810-193 AveiroPortugal CESAM Department of ChemistryUniversity of Aveiro3810-193 AveiroPortugal LAQV-REQUIMTE Department of Chemistry&Central Laboratory of AnalysisUniversity of Aveiro3810-193 AveiroPortugal CIIMAR一Interdisciplinary Centre of Marine and Environmental Research MatosinhosPortugal N9VE-Nature Ocean and ValueLdaPortoPortugal Central Laboratory of Analysis University of Aveiro3810-193 AveiroPortugal

Sorption has been proposed as a promising approach towards a sustainable water decontamination and recently to the recycling of rare earth elements(REE).Although living seaweed have been shown to be capable of removing REE from contaminated solutions,no studies have yet compared the effects of REE competition on sorption onto different groups of *** effects were analysed in the present study by exposing six living seaweeds(Ulva lactuca,Ulva intestinalis,Fucus spiralis,Fucus vesiculosus,Gracilaria *** Osmundea pinnatifida)(3 g/L,fresh weight) to mono-element and multi-element solutions of Y,La,Ce,Pr,Nd,Eu,Gd,Tb and Dy(1 μmol/L).Results show a preference towards light REE in mono-element solutions,which is reduced when in competition with other *** competitive effect is less pronounced in heavy REE indicating that these are still able to bind to the macroalgae despite the presence of competing *** to water content(%),seaweed specific surface area(cm^(2)/g) shows to be an important factor in the sorption of REE,since larger surface area is associated with higher removal and larger competitive effect.

关键词： Bioaccumulation Wastewater e-waste Recovery Removal Marine macroalgae

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Co-nanoencapsulated meloxicam and curcumin improves cognitive impairment induced by amyloid-beta through modulation of cyclooxygenase-2 in mice

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Neural Regeneration Research 2021年第4期16卷 780-786页

作者： Maria Eduarda Ziani Gutierrez Anne Suély pinto Savall Edina da Luz Abreu Kelly Ayumi Nakama Renata Bem Dos Santos Marina costa Monteiro Guedes daiana SilvaÁvila Cristiane Luchese Sandra Elisa Haas Caroline Brandão Quines Simone pinton Postgraduation Program in Biochemistry Federal University of Pampa(UNIPAMPA)UruguaianaRSBrazil Postgraduation Program in Pharmaceutical Science Federal University of Pampa(UNIPAMPA)UruguaianaRSBrazil Postgraduation Program in Biochemistry and Bioprospecting Federal University of Pelotas(UFPEL)Capão do LeãoRSBrazil

Alzheimer’s disease is a progressive brain disorder and complex mechanisms are involved in the physiopathology of Alzheimer’s ***,there is data suggesting that inflammation plays a role in its development and ***,some non-steroidal antiinflammatory drugs,such as meloxicam,which act by inhibiting cyclooxygenase-2 have been used as neuroprotective agents in different neurodegenerative disease *** purpose of this study was to investigate the effects of co-nanoencapsulated curcumin and meloxicam in lipid core nanocapsules(LCN)on cognitive impairment induced by amyloid-beta peptide injection in *** were prepared by the nanoprecipitation *** Swiss mice received a single intracerebroventricular injection of amyloid-beta peptide aggregates(fragment 25–35,3 nmol/3μL)or vehicle and were subsequently treated with curcumin-loaded LCN(10 mg/kg)or meloxicam-loaded LCN(5 mg/kg)or meloxicam+curcumin-co-loaded LCN(5 and 10 mg/kg,respectively).Treatments were given on alternate days for 12 days(i.e.,six doses,once every 48 hours,by intragastric gavage).Our data showed that amyloid-beta peptide infusion caused long-term memory deficits in the inhibitory avoidance and object recognition tests in *** the inhibitory avoidance test,both meloxicam and curcumin formulations(oil or co-loaded LCN)improved amyloid-beta-induced memory impairment in ***,only meloxicam and curcumin-co-loaded LCN attenuated non-aversive memory impairment in the object recognition ***,the beneficial effects of meloxicam and curcuminco-loaded LCN could be explained by the anti-inflammatory properties of these drugs through cortical cyclooxygenase-2 *** study suggests that the neuroprotective potential of meloxicam and curcumin co-nanoencapsulation is associated with cortical cyclooxygenase-2 *** study was approved by the Committee on Care and Use of Experimental Animal Resources,the Federal University of Pampa,Brazil(app

关键词： Alzheimer’s disease curcumin cyclooxygenase-2 lipid core nanocapsules meloxicam memory rats inflammation

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“Love Is Strong, and You＇re so Sweet”： JAGGER Is Essential for Persistent Synergid Degeneration and Polytubey Block in Arabidopsis thaliana

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Molecular Plant 2016年第4期9卷 601-614页

作者： Ana Marta Pereira Margarida Sofia Nobre Sara Cristina pinto Ana Lucia Lopes Mario Luis costa Simona Masiero Silvia Coimbra Departamento de Biologia Faculdade de Ciencias da Universidade do Porto Rua do Campo Alegre 4169-007 Porto Portugal Plant Functional Genomics Group Biosystems and Integrative Sciences Institute (BiolSI) 4169-007 Porto Portugal Dipartimento di BioScienze Universita degli Studi di Milano 20133 Milan Italy Present address: Institute of Plant Biology and ZUrich-Basel Plant Science Center University of ZUrich Zolikerstrasse 107 8008 Zurich Switzerland

Successful double fertilization and subsequent seed development in flowering plants requires the delivery of two sperm cells, transported by a pollen tube, into the embryo sac of an ovule. The embryo sac cells tightly control synergid cell death, and as a result the polyspermy block. Arabinogalactan proteins are highly glycosylated proteins thought to be involved in several steps of the reproductive process. We show that JAGGER, Arabinogalactan Protein 4, is an important molecule necessary to prevent the growth of multiple pollen tubes into one embryo sac in Arabidopsis thaliana. In jagger, an AGP4 knockout mutant, the pistils show impaired pollen tube blockage as a consequence of the survival of the persistent synergid. JAGGER seems to be involved in the signaling pathway that leads to a blockage of pollen tube attraction. Our results shed light on the mechanism responsible for preventing polyspermy in Arabidopsis and for safe- guarding successful fertilization of all ovules in one pistil, ensuring seed set and the next generation.

关键词： Polytubey persistent synergid arabinogalactan proteins polyspermy

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