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Journal of Biosciences and Medicines 2019年第3期7卷 92-101页

作者： Lorrayne Clemente Ribeiro de Sousa Almir Ribeiro de Carvalho Junior Mario Geraldo de Carvalho Tania Maria Sarmento da Silva rafaela oliveira ferreira Universidade Federal do Tocantins Gurupi Brazil Instituto Federal de Santa Catarina Criciúma Brazil Universidade Federal Rural do Rio de Janeiro Seropédica Brazil Universidade Federal Rural de Pernambuco Recife Brazil

This study describes the phytochemical profile and antioxidant activity of an extract from the leaves of Pouteria caimito (Sapotaceae). The extract in ethanol was obtained by maceration at room temperature and subjected to the liquid-liquid partition to obtain fractions in hexane and ethyl acetate. Steroids, triterpenes, saponins, alkaloids and flavonoids were identified by the phytochemical prospection of extracts and fractions from the leaves. The analysis of the ethyl acetate fraction by UPLC-QTOF-MS allowed us to identify eight triterpenes, namely, euscaphic acid (1), hyptadienic acid (2), betulinic acid (3), oleanolic acid (4), ursolic acid (5), 3β-(O-p-coumaroyl)- alphitolic acid (6), 3β-(O-p-coumaroyl)-maslinic acid (7) and 3β-(O-p-coumaroyl)-2-hydroxy-urs-12-en-28-oic acid (8). The ethanol extract and ethyl acetate fraction presented total phenolic contents of 10.6 ± 0.1 and 11.4 ± 0.3 mg GAE g-1, respectively, and considerable antioxidant activity in the DPPH assay with EC50 values of 299.4 ± 1.5 and 391.8 ± 0.9 μg·ml-1, respectively.

关键词： Abiu UPLC Triterpenes DPPH Antioxidants

Bifid pancreatic tail as cause of acute pancreatitis

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Hepatobiliary & Pancreatic Diseases International 2018年第5期17卷 480-481页

作者： Hanna rafaela ferreira Dalla Pria Rafael Andrade Santiago Fernanda Garozzo Velloni Giuseppe D’ippolito Department of Imaging Universidade Federal de Sao PauloSao PauloBrazil Diagnos ticos da Ametica S.A.DASA. Sao PauloBrazil Grupo Fleury Sao PauloBrazil

To the Editor:Wepresentacaseofa22-year-oldwomanwithrecurrent episodes of acute pancreatitis. The patient had no known risk factors such as history of alcoholism, smoking, pre-existing medical conditions, family member... 详细信息

关键词： pre-existing medicall dilatation or cholelithiasis

Near-infrared leaf reflectance modeling of Annona emarginata seedlings for early detection of variations in nitrogen concentration

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Journal of Forestry Research 2023年第1期34卷 269-282页

作者： rafaela Lancas Gomes Marília Caixeta Sousa Felipe Girotto Campos Carmen Sílvia Fernandes Boaro José Raimundo de Souza Passos Gisela ferreira Bioestatistic Plant BiologyParasitology and Zoology DepartmentBioscience Institute(IBB)Universidade Estadual Paulista(UNESP)BotucatuSao PauloBrazil

Nitrogen(N)monitoring is essential in nurseries to ensure the production of high-quality *** spectroscopy(NIRS)is an instantaneous,nondestructive method to monitor *** data such as NIRS can also provide the basis for developing a new vegetation spectral index(VSI).Here,we evaluated whether NIRS combined with statistical modeling can accurately detect early variations in N concentration in leaves of young plants of Annona emargiaata and developed a new VSI for this *** were grown in a hydroponics system with 0,2.75,5.5or 11 mM N for 45 *** we measured gas exchange,chlorophylla fluorescence,and pigments in leaves;analyzed complete leaf nutrients,and recorded spectral data for leaves at 966 to 1685 nm using *** a statistical learning approach,the dimensionality of the spectral data was reduced,then models were generated using two classes(N deficiency,N)or four classes(0,2.75,5.5,11 mM N).The best combination of techniques for dimensionality reduction and classification,respectively,was stepwise regression(PROC STEPDISC)and linear discriminant *** was possible to detect N deficiency in seedlings leaves with 100%precision,and the four N concentrations with93.55%accuracy before photosynthetic damage to the plant ***,NIRS combined with statistical modeling of multidimensional data is effective for detecting N variations in seedlings leaves of ***.

关键词： Mineral nutrition of plants Near-infrared spectroscopy Spectral vegetation index Digital signature Statistical learning Fluorescence of chlorophylla

Phenolic profile and biological potential of Endopleura uchi extracts

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Asian Pacific Journal of Tropical Medicine 2015年第11期8卷 870-878页

作者： Luis R.Silva rafaela Teixeira CICS-UBI-Centro de Investigacao em Ciencias da Saude.Universidale da Beira Interior Av.Infante D.Henrique.6201-506.Corilha.Portugal Instituto Politecnico de Castelo Branco Escola Superior de Saude Dr Lopes DiasArenido do EmpresarioCampus da Talagueira6000-767Castelo BrancaPortugal LEPABE Departamento de Engenharia QuimicaFaculdade de Engenharia.Universidade do PortoRua Dr.Roberto Friass/n4200-465 PortoPortugal REQUIMTE/Laboratorio de Farmacognosia Departamento de QuimicaFaculdade de FarmaciaUniversidade do Porto.Rua de Jorge Viterbo Ferreira228.4050-313 PortoPortugal

Objective:To improve the knowledge on the metabolite composition of Endopleura uchi bark,concerning phenolic compounds,and to evaluate some of its biological capacities for further possible exploitation in food and pharmaceutical industries associated to their healthpromoting ***:Two different extracts(infusion and hydroethanolic) were studied concerning phenolic composition and biological ***:Five compounds were determined by HPLC-DAD,being bergenin the major *** general way,infusion presents a greater richness in these *** antioxidant,***,α-glucosidase and antibacterial activity were checked by in vitro assays.A dose-dependent response was noticed against DPPH,superoxide and nitric oxide radicals,acetylcholinesterase,butyrylcholinesterase and against α-glucosidase inhibitory *** capacity of both extracts was investigated against Gram-positive and Gramnegative bacteria,being more effective against the first *** concentrations of infusion extract tested here revealed that is non-toxic for intestinal(Caco-2) cells ***:The results suggest that the extracts of Endopleura uchi may be interesting for incorporating in pharmaceutical preparations for human health,since it can suppress hyperglycaemia and inhibit cholinesteases,and or as food additive due to its antiradical and antibacterial activities.

关键词： Endopleura uchi Bark Phenolic compounds Anti-diabe

Initial Growth and Gas Exchanges of Plants of Colored Cotton Submitted to Saline Stress

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Agricultural Sciences 2018年第12期9卷 1652-1663页

作者： Hallyson oliveira Ronaldo do Nascimento Samuel Silva José Alberto ferreira Cardoso rafaela Felix Basílio Guimaraes Elka Costa Santos Nascimento Post-Graduated of Agricultural Engineer Federal University of Campina GrandeCampina GrandeBrazil

The objective of the research was to evaluate the effects of saline stress on the initial growth and physiological parameters of three varieties of cotton plants, being two cultivars of colored fiber (BRS Topázio and BRS Verde) and one of white fiber (BRS 286). The experiment was conducted under greenhouse conditions. After chemical depletion with sulfuric acid, five seeds were seeded in polyethylene pots of 20 kg, leaving only one plant per pot after thinning. The experimental design was completely randomized, with a factorial scheme of 3 × 5, with five replications, totaling 75 plots, being three cotton varieties and five saline stress treatments—1.1;3.1;5.1;7.1;9.1 dS m−1. The evaluations occurred at 15 and 30 days after the beginning of the treatments (DAT) for growth variables (plant height, stem diameter, number of leaves and leaf area) and gas exchange (stomatal conductance, net photosynthesis rate and transpiration. The variety that was most resistant to the salt stress levels studied was BRS 286, followed by BRS Topazio and BRS Verde.

关键词： Photosynthesis Stomatal Conductance Dry Mass Resistance Cultivars

Essential Oils from Mentha piperita,Cymbopogon citratus,Rosmarinus officinalis,Peumus boldus and Foeniculum vulgare:Inhibition of Phospholipase A_(2) and Cytotoxicity to Human Erythrocytes

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American Journal of Plant Sciences 2017年第9期8卷 2196-2207页

作者： Danubia Aparecida de Carvalho Selvati Rezende Maria das Gracas Cardoso rafaela Vieira Souza Maria Luisa Teixeira rafaela Magalhaes Brandao Vanuzia Rodrigues Fernandes ferreira Jéssica oliveira e Nogueira Maisa Lamounier Magalhaes Silvana Marcussi David Lee Nelson Departamento de Química Universidade Federal de Lavras Lavras Brasil Departamento de Ciência dos Alimentos Universidade Federal de Lavras Lavras Brasil Universidade Federal do Vale do Jequitinhonha e Mucuri Diamantina Brasil

The essential oils from Mentha piperita, Cymbopogon citratus, Rosmarinus officinalis, Peumus boldus and Foeniculum vulgare were extracted by hydrodistillation and characterized and quantified by GC-MS and GC-DIC. The oils induced hemolysis with all the doses evaluated (0.6 to 1.8 μL), and the diameters of the halos varied between 9 and 15 mm. Pre-incubation of P. boldus oil with Bothrops jararacussu venom resulted in potentiation of venom-induced hemolysis (30%) (proteases and phospholipases A2). The essential oil from M. piperita (0.6 μL) inhibited venom-induced hemolysis by 45%, whereas 0.6 μL of R. officinalis oil increased the hemolysis by 20%. For the essential oil from F. vulgare, 100% inhibition of activity (0.6 and 1.2 μL) was observed. The application of C. citratus oil induced hemolysis with all the volumes evaluated. Phospholipase activity induced by the venom was only inhibited (10%) with the 0.6 μL volume of R. officinalis oil. The oils from M. piperita and F. vulgare (1.8 μL) and C. citratus oil (0.6 μL) potentiated the phospholipase activity. The results highlight the need for a broad characterization and regulation of the use of natural products, because they can have therapeutic or toxic actions.

关键词： Natural Products Snake Venoms Hemolysis Pharmacological Potential

Esophageal stenosis with sloughing esophagitis:A curious manifestation of graft-vs-host disease

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World Journal of Gastroenterology 2015年第30期21卷 9217-9222页

作者： Daniel Trabulo Sara ferreira Pedro Lage rafaela Lima Rego Gilda Teixeira A Dias Pereira Gastroenterology Department Hospital de S?o BernardoCentro Hospitalar de Setúbal E.P.E.2910-446 SetúbalPortugal Gastroenterology Department Instituto Português de Oncologia de Lisboa de Francisco Gentil Pathology Department Instituto Português de Oncologia de Lisboa de Francisco Gentil Hemathology Department Instituto Português de Oncologia de Lisboa de Francisco Gentil

We report a case of a 56-year-old woman with a history of allogenic bone marrow transplantation for two years,complaining with dysphagia and weight loss. Upper endoscopy revealed esophageal stenosis and extensive mucosa sloughing. Biopsies confirmed the diagnosis of graft-vs-host disease(GVHD). Balloon dilation,corticosteroids and cyclosporin resulted in marked clinical improvement. Gastrointestinal tract is involved in the majority of patients with chronic GVHD. Esophageal manifestations are rare and include vesiculobullous disease,ulceration,esophageal webs,casts or strictures. Sloughing esophagitis along with severe stenosis requiring endoscopic dilation has never been reported in this context.

关键词： Dysphagia Esophageal stenosis Sloughingesophagitis Balloon dilation Graft-vs -host-disease

Evaluation of the Coagulating Potential of the Crude Extract from the Barbatimão Bark for the Treatment of Dairy Effluents

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American Journal of Plant Sciences 2016年第13期7卷 1685-1692页

作者： Vanuzia Rodrigues Fernandes ferreira Josefina Aparecida de Souza Maria das Graças Cardoso Luiz Gustavo de Lima Guimarães rafaela Magalhães Brandão rafaela Vieira Souza Luana Isac Soares Jéssica oliveira e Nogueira Francielli D’Carlos Cravo David Lee Nelson Department of Chemistry Federal University of Lavras Lavras Brazil Federal University of Sao Joao Del Rei Sao Joao Del Rei Brasil Pro-Reitoria de Pesquisa e Pós-Graduacao Federal University of Vales de Jequitinhonha and Mucuri Diamantina Brazil

The coagulating potential of the crude extract from the barbatimao bark for the treatment of dairy effluent was assessed and compared with industrial coagulants such as aluminum sulfate (inorganic coagulant), the crude extract of black wattle bark (used in the manufacture of natural organic coagulants), and gallic acid (the main constituent of condensed tannins). The aqueous extract of barbatimao was obtained by refluxing for 12 h, and the black wattle was provided by a producer of natural coagulants. The determinations of total phenolic compounds and tannins in the extracts were performed by the Folin-Ciocalteu method and precipitation with casein, respectively. The treatment of the effluent with coagulant was performed by the Jar-Test. After testing, the effluent was evaluated for turbidity, total solids, and biochemical oxygen demand (BOD). A higher content of phenolic compounds (19.81% mg of gallic acid/g extract) was observed in the barbatimao extract than in the black wattle extract (14.24% mg of gallic acid/g extract). However, the extracts contained the same amount of tannins, 13.95% for the barbatimao extract and 12.89% for the black wattle extract. A 63%, reduction in BOD was observed for the effluent treated with barbatimao extract, 35% for the black wattle extract, 58% for gallic acid and 60.59% for the treatment with aluminum sulfate. Treatment with gallic acid caused a 98% reduction in the turbidity of the effluent;with aluminum sulfate, a 97% reduction was obtained;with barbatimao extract, 96%, and with black wattle extract, 93%. The barbatimao extract was shown to be a potential coagulant for dairy effluents.

关键词： Stryphnodendron adstringens Acacia mearnsii Tannins

Influence of Cold Storage on the Bioactivity Properties and the Quality of the Juice of Moro Blood Orange(Citrus sinensis(L.)Osbeck)

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American Journal of Plant Sciences 2019年第1期10卷 24-37页

作者： Maisa Lamounier Magalhaes Luiz Carlos De oliveira Lima Allan da Silva Lunguinho Danubia Aparecida De Carvalho Selvati Rezende Vanuzia Rodrigues Fernandes ferreira rafaela Magalhaes Brandao Josefina Aparecida De Souza Ellen Cristina De Souza Kátia Júlia De Almeida David Lee Nelson Maria Das Gracas Cardoso Departamento de Ciencia dos Alimentos Universidade Federal de Lavras(UFLA)LavrasBrazil Departamento de Química Universidade Federal de Lavras(UFLA)LavrasBrazil Programa de Pos-Graduacao em Biocombustívis Universidade Federal do Vale do Jequitinhonha e MucuriDiamantinaBrazil

The possibility of commercialization of Moro blood oranges in tropical countries such as Brazil was evaluated to verify whether post-harvest management through storage at low temperatures for a period of 60 days can improve the bioactive properties and quality parameters. Moro blood oranges cultivated in Brazil did not contain significant amounts of anthocyanins at the time of harvesting, but these compounds were activated by post-harvest management through storage at low temperatures (4°C and 8°C) for a period of 60 days. The emergence of the anthocyanins in the juices occurred within a few weeks of storage, but the maximum levels were attained after 60 days and at the temperature of 8°C. Cold storage positively influenced other bioactive compounds such as total phenolic compounds, individual phenolic compounds, β-carotene and the antioxidant activity determined by the sequestration of DPPH free radicals. It did not influence the vitamin C content. In addition, storage significantly altered the color, total acidity and pH of the fruits, but it did not prevent its commercial use. The remaining quality parameters were not influenced. It is possible to commercialize these oranges in Brazil through post-harvest management.

关键词： Cyanidin-3-Glycoside Antioxidant Post-Harvest

Chemical Characterization and in Vitro Antitumor Activity of the Essential Oils from the Leaves and Flowers of Callistemon viminalis

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American Journal of Plant Sciences 2015年第16期6卷 2664-2671页

作者： Christiane Maria de oliveira Maria das Gracas Cardoso Marisa Ionta Marisi Gomes Soares Juliana de Andrade Santiago Guilherme álvaro ferreira da Silva Maria Luisa Teixeira Danúbia Aparecida de Carvalho Selvati Rezende rafaela Vieira de Souza Luana Isac Soares David Lee Nelson Marcos Schleiden Sousa Carvalho Department of Chemistry Federal University of LavrasLavrasBrazil Institute of Biomedical Sciences Federal University of AlfenasAlfenasBrazil Department of Chemistry Federal University of AlfenasAlfenasBrazil Federal University of Vales de Jequitinhonha e Mucuri DiamantinaBrazil Department of Agriculture Federal University of LavrasLavrasBrazil

The objective of this study was to characterize and verify the in vitro antitumor activity of essential oils (EOs) extracted from the leaves and flowers of Callistemon viminalis. The EOs were extracted by hydrodistillation using a modified Clevenger apparatus. The identification and quantification of constituents were performed on a gas chromatograph coupled to a mass spectrometer and a gas chromatograph with a flame ionization detector. The antitumoral activity was evaluated by a colorimetric assay (MTS) using different cell lines derived from human tumors (breast, lung, glioblastoma, and melanoma). The major constituents of the EOs of leaves and flowers were similar, only quantitative differences being observed. The compounds 1,8-cineole, α-pinene and α-terpineol were found in concentrations of 50.4%, 25.8% and 8.7% in the EOs obtained from the leaves and 48.8%, 24.5% and 3.9% in the EOs obtained from the flowers, respectively. The cytotoxic activity of the EOs was observed only in melanoma cultures (HT144). Cultures treated for 48 h with EOs from leaves and flowers (200 μg·mL-1) reduced the viability by 40% and 25%, respectively. Thus, the antiproliferative activity of the EO from leaves was more pronounced than the EO from flowers in cells derived from melanoma.

关键词： Cancer Volatile Oil Escova-de-Garrafa(Bottle Brush)