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Development of a multiplex polymerase chain reaction assay for detection of hepatitis C virus,hepatitis B virus,and human immunodeficiency virus 1

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World Journal of Virology 2024年第1期13卷 95-106页

作者： Waleed Abdelgaber Nemr radwan k nashwa Department of Radiation Microbiology National Center for Radiation Research and TechnologyEgyptian Atomic Energy AuthorityCairo 11371Egypt Department of Health Radiation Research National Center for Radiation Research and TechnologyEgyptian Atomic Energy AuthorityCairo 11371Egypt

BACkGROUND Hepatitis C virus(HCV),hepatitis B virus(HBV),and human immunodeficiency virus 1(HIV-1)are the most epidemic blood-borne viruses,posing threats to human health and causing economic losses to nations for combating the infection *** diagnostic methodologies that depend on the detection of viral nucleic acids are much more expensive,but they are more accurate than sero-logical *** To develop a rapid,cost-effective,and accurate diagnostic multiplex polymerase chain reaction(PCR)assay for simultaneous detection of HCV,HBV,and *** The design of the proposed PCR assay targets the amplification of a short conserved region featured with a distinguishable melting profile and electro-phoretic molecular weight inside each viral ***,this diagnostic method will be appropriate for application in both conventional(combined with electrophoresis)and real-time PCR *** in silico investigations were conducted to prove the capability of the approached PCR assay to detect variants of each ***,Egyptian isolates of each virus were subjected to the wet lab examination using the given diagnostic *** The in silico investigations confirmed that the PCR primers can match many viral variants in a multiplex PCR *** wet lab experiment proved the efficiency of the assay in distinguishing each viral type through high-resolution melting *** to related published assays,the proposed assay in the current study is more sensitive and competitive with many expensive PCR *** This study provides a simple,cost-effective,and sensitive diagnostic PCR assay facilitating the detection of the most epidemic blood-borne viruses;this makes the proposed assay promising to be substitutive for the mistakable and cheap serological-based assays.

关键词： Diagnosis Blood-borne viruses Multiplex polymerase chain reaction High-resolution melting

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Effectiveness of health coaching on diabetic patients: A systematic review and meta-analysis

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Traditional Medicine Research 2019年第6期4卷 314-325页

作者： nashwa Mohamed radwan Hisham Al khashan Fahad Alamri Ahmed Tofek El Olemy Department of Public Health and Community Medicine Tanta UniversityTantaEgypt Consultant in Clinical Education Department Ministry of HealthRiyadhKingdom of Saudi Arabia Family Medicine Consultant Ministry of HealthRiyadhKingdom of Saudi Arabia National Center for Complementary and Alternative Medicine Ministry of HealthRiyadhKingdom of Saudi Arabia.

、Background:Using health coaching to improve the quality of life and health outcomes of the patients with diabetes mellitus,has emerged as a possible ***,the few published randomized controlled trials using health coaching for patients with diabetes mellitus have reported mixed *** present meta-analysis aimed to determine the effectiveness of health coaching on modifying health status and quality of life among diabetic patients and to clarify the characteristics of coaching delivery that make it most ***:This study searched for articles on randomized controlled trials of health coaching interventions targeting type 2 diabetic patients that were published in the English language from January 2005 through December 2018 in the Cochrane,Medline,PubMed,Trip,and Embase *** in the control group received usual diabetes mellitus care,and those in the experimental group received health coaching based on usual diabetes mellitus *** primary outcomes included Hemoglobin A1c(HbA1c)and cardiovascular disease risk factors,including systolic blood pressure,diastolic blood pressure,triglyceride,high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol,total cholesterol,and body *** secondary outcomes included quality of life,self-efficacy,self-care skills,and psychological ***:Health coaching intervention has a significant effect on HbA1c[mean difference(MD)=-0.35,confidence interval(CI)=-0.47,-0.22,I2=83%,P<0.001]and HDL-C(MD=-0.50,CI=-0.93,-0.07,I2=10%,P=0.02).The most effective strategy for health coaching delivery associated with improvement of HbA1c was decreasing the number of sessions and increasing the duration of each ***,no significant difference was found for weight,SBP,diastolic blood pressure,triglyceride,low-density lipoprotein cholesterol,or total *** results were reported for the effect of health coaching on quality of life,self-efficacy,self-

关键词： Health coaching Type 2 diabetes mellitus Randomized controlled trials Hemoglobin A1c Weight High-density lipoprotein cholesterol

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