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多头带绦虫TPx基因片段的克隆及原核表达

中国兽医科学 2009年第3期39卷 251-256页

作者：李永光李文卉李航盖文燕王鸿盛姚菊霞王艳华张德林贾万忠 radu blaga 付宝权甘肃农业大学动物医学院中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部兽医公共卫生重点开放实验室甘肃省动物寄生虫病重点实验室 College of Veterinary Medicine University of Agricultural Sciences and Veterinary MedicineCluj-Napoca 400372Romania

从自然感染的病羊脑内采集多头蚴原头节,提取总RNA,采用RT-PCR技术扩增多头带绦虫硫氧还蛋白过氧化物酶(TmTPx)基因片段,PCR产物连接到pMD18-T载体,转化至大肠杆菌DH5α后测序,发现克隆到的TmTPx基因片段开放阅读框为453 bp,编码150个... 详细信息

从自然感染的病羊脑内采集多头蚴原头节,提取总RNA,采用RT-PCR技术扩增多头带绦虫硫氧还蛋白过氧化物酶(TmTPx)基因片段,PCR产物连接到pMD18-T载体,转化至大肠杆菌DH5α后测序,发现克隆到的TmTPx基因片段开放阅读框为453 bp,编码150个氨基酸。对TmTPx基因片段进行限制性酶切后连接到表达载体pGEX-4T-1,构建重组表达质粒pGEX-4T-TmTPx,转化大肠杆菌DH5α后筛选阳性克隆,经限制性酶切分析、PCR及测序鉴定后,转化大肠杆菌BL21,以IPTG进行诱导表达,用SDS-PAGE分析表达产物。结果显示,成功表达了大小约为43 ku的融合蛋白。对表达产物纯化后免疫家兔,采集血清用ELISA测定抗体效价,发现兔抗TmTPx重组蛋白的抗体能够与多头蚴原头节抗原发生特异性反应,说明该重组蛋白具有较好的免疫原性。

关键词：多头带绦虫多头蚴硫氧还蛋白过氧化物酶重组蛋白免疫原性

多头带绦虫多头蚴cDNA表达文库的构建及初步分析

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多头带绦虫多头蚴cDNA表达文库的构建及初步分析

中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者：李文卉盖文燕王鸿盛姚菊霞曲自刚王艳华张德林贾万忠 radu blaga 付宝权中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部兽医公共卫生重点开放实验室甘肃省动物寄生虫病重点实验室 University of Agricultural Sciences and Veterinary Medicine Faculty of Veterinary Medicine

本研究从甘肃景泰羊源多头蚴原头节提取总RNA,以Oligo d(T)纤维素柱纯化mRNA,利用Lambda ZAPIIXR文库构建试剂盒构建了多头蚴cDNA表达文库。从构建的原始文库随机挑选单个噬菌斑进行PCR鉴定及测序分析,确定文库重组率和插入外源基因片... 详细信息

本研究从甘肃景泰羊源多头蚴原头节提取总RNA,以Oligo d(T)纤维素柱纯化mRNA,利用Lambda ZAPIIXR文库构建试剂盒构建了多头蚴cDNA表达文库。从构建的原始文库随机挑选单个噬菌斑进行PCR鉴定及测序分析,确定文库重组率和插入外源基因片段大小。结果表明多头蚴cDNA表达文库的原始库容量为1.0x10噬

关键词：多头带绦虫脑多头蚴 cDNA表达文库 EST

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应用PCR技术鉴定我国旋毛虫分离株的研究

应用PCR技术鉴定我国旋毛虫分离株的研究

多头带绦虫脑多头蚴cDNA表达文库的构建及初步分析

中国畜牧兽医学会家畜寄生虫学分会第六次代表大会暨第十次学术研讨会

作者：付宝权刘明远姚春雨李文卉李永光王艳华吴秀萍张德林才学鹏 radu blaga Pascal Boireau 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部兽医公共卫生重点开放实验室甘肃省动物寄生虫病重点实验室吉林大学人兽共患病研究所内蒙古民族大学动物科技学院 University of Agricultural Sciences and Veterinary Medicine Faculty of Veterinary MedicineCluj-Napoca

根据旋毛虫***(T1)和乡土旋毛虫***(T2)的线粒体核糖体小亚基及大亚基RNA基因序列设计合成特异性引物,同时合成旋毛虫核糖体扩展片段V特异引物,以旋毛虫***和乡土旋毛虫***标准株为对照,对我国10个旋毛虫分离株进行了PCR鉴定。结果表明... 详细信息

根据旋毛虫***(T1)和乡土旋毛虫***(T2)的线粒体核糖体小亚基及大亚基RNA基因序列设计合成特异性引物,同时合成旋毛虫核糖体扩展片段V特异引物,以旋毛虫***和乡土旋毛虫***标准株为对照,对我国10个旋毛虫分离株进行了PCR鉴定。结果表明,河南猪株、西安猪株、云南猪株、哈尔滨猪株、天津猪株、乌兰浩特犬株1旋毛虫的PCR扩增条带与T1一致,为旋毛虫***;而长春犬株与鸟兰浩特犬株2旋毛虫的PCR扩增条带与T2一致,为乡土旋毛虫***。同时对以上旋毛虫分离株5S rDNA内部基因间隔区序列进行测定,序列比对分析结果也证实这十个分离株分别属于旋毛虫***和乡土旋毛虫***,而且不同分离株5S RNA内部转录间隔区基因序列存在差异。建立的PCR方法可以应用于我国旋毛虫分离株的虫种分子分类鉴定,这对我国旋毛虫病分子病原学检测、分离株的分类鉴定以及种群遗传结构的研究都具有重要意义。

关键词：中国旋毛虫分离株 PCR 鉴定

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寄生虫与医学昆虫学报 2011年第3期18卷 139-146页

作者：李文卉王建魁盖文燕姚菊霞曲自刚贾万忠罗建勋 radublaga 付宝权甘肃农业大学动物医学院兰州730070 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部兽医公共卫生重点开放实验室甘肃省动物寄生虫病重点实验室兰州730046 兰州市七里河区动物疫病预防控制中心兰州730050 UniversityofAgriculturalSciencesandVeterinaryMedicine Cluj-Napoca400372Romania

从甘肃景泰羊源脑多头蚴原头节提取总RNA，以Oligo（dT）纤维素柱纯化mRNA，利用LambdaZAPIIXR文库构建试剂盒构建了脑多头蚴eDNA表达文库。从构建的原始文库随机挑选单个噬菌斑进行PCR，确定文库重组率和插入外源基因片段大小，鉴定文... 详细信息

从甘肃景泰羊源脑多头蚴原头节提取总RNA，以Oligo（dT）纤维素柱纯化mRNA，利用LambdaZAPIIXR文库构建试剂盒构建了脑多头蚴eDNA表达文库。从构建的原始文库随机挑选单个噬菌斑进行PCR，确定文库重组率和插入外源基因片段大小，鉴定文库质量。结果表明脑多头蚴eDNA表达文库的原始库容量为1．0×10。pfu，扩增后文库滴度为1．6×10^9pfu／mL。随机挑选的165个噬菌斑克隆中，0．25kb以上的克隆155个，重组率为93．9％，对其中0．5kb以上的115个克隆测序共获得104个表达序列标签（EST），分析后得到96个单一EST序列（UniqueEST），其中20个EST与绦虫基因有同源性，38个EST与吸虫基因有同源性，4个EST与线虫基因有同源性，17个EST与其他物种有同源性，其余17个EST没有同源基因。这些EST编码的氨基酸序列有多头带绦虫六钩蚴Tml6抗原、猪带绦虫副肌球蛋白、猪带绦虫免疫原蛋白Ts76等绦虫抗原的同源蛋白以及烯醇化酶等一些酶类、热休克蛋白、肌动蛋白、核糖体蛋白等同源蛋白。

关键词：多头带绦虫脑多头蚴 cDNA表达文库表达序列标签

Psychosocial determinants of irritable bowel syndrome

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World Journal of Gastroenterology 2012年第7期18卷 616-626页

作者： Teodora Surdea-blaga Adriana Bban Dan L Dumitrascu 2nd Medical Department University of Medicine and Pharmacy IuliuHatieganuCluj-Napoca 400006Romania Department of Psychology Babes-Bolyai UniversityCluj-Napoca 400006Romania

From a pure motor disorder of the bowel,in the past few years,irritable bowel syndrome (IBS) has become a multifactorial disease that implies visceral hypersensitivity,alterations at the level of nervous and humoral communications between the enteric nervous system and the central nervous system,alteration of the gut microflora,an increased intestinal permeability and minimum intestinal *** and social factors can interfere with the communication between the central and enteric nervous systems,and there is proof that they are involved in the onset of IBS and influence the response to treatment and *** is evidence that abuse history and stressful life events are involved in the onset of functional gastrointestinal *** order to explain clustering of IBS in families,genetic factors and social learning mechanisms have been *** psychological features,such as anxiety,depression as well as the comorbid psychiatric disorders,health beliefs and coping of patients with IBS are discussed in relation to the symptoms and outcome.

关键词： Anxiety Depressive symptoms Irritablebowel syndrome Personality Psychosocial factors Sexual abuse Stressful events

Convergence and Error of Some Numerical Methods for Solving a Convection-Diffusion Problem

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Applied Mathematics 2013年第5期4卷 72-79页

作者： Gabriela Nut Ioana Chiorean Petru blaga Applied Mathematics Department Babes-Bolyai University Cluj Napoca Romania

We use the local Fourier analysis to determine the properties of the multigrid method when used in modeling the skin penetration of a drug. The analyses of these properties can be very in designing an efficient structure of the multigrid method and in comparing the element and finite difference discretization techniques. After the theoretical results obtained, we also present some numerical results for a problem for which the solution is known.

关键词： Time Dependent Convection Diffusion Multigrid Method Finite Element and Finite Differences Discretization

Vascular Flora from Ecoton Zones of the Slatioara Secular Forest

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Open Journal of Forestry 2023年第1期13卷 75-91页

作者： Marius Dan Șandru Camelia-Maria Golea Dumitru Dorel blaga Genebank “Mihai Cristea” Suceava Romania Stefan cel Mare University of Suceava Suceava Romania

The purpose of this study is to generate essential scientific information regarding the floristic composition and the structures of the ecotone vegetation in the natural forest Codrul Secular Slătioara, because so far no such studies have been carried out in this reserve. Also, the results of this study provide important data, which can be the basis for the development of strategies for the conservation and sustainable use of some herbaceous species, valuable due to genetic and socio-economic, respectively of a regular forest monitoring program. The floristic studies were carried out in the 2020 to 2021 vegetation season. Based on the specialized literature, plant species were identified and then processed from the point of view of bioforms, floristic elements and ecological indices. The economic categories in which the identified species fall were also analyzed. Thus, a total of 292 species belonging to 46 botanical families were identified and their distribution by ecosystems is as follows: in the forest, 90 species were identified, in the lands adjacent to the forest, in the meadows, 202 species were found and in the area of border, 120 species of vascular plants were identified. Most of the species identified in the study area are hemicryptophytes, heliophiles, and eurytherms, which grow on dry to moderately moist, eurytrophic soils. More than 50% of the identified species belong to several categories of useful plants (fodder, medicinal, melliferous, edible).

关键词： Flora Border Bioforms Floristic Elements Ecological Indices Economic Cat-egories

Scientific Research Complex ＂BeOn-1＂ Performance Indicator of the Operators in Extreme Conditions

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Journal of Earth Science and Engineering 2014年第11期4卷 675-683页

作者： Stoyan Tanev Plamen Trendafilov Hristo Hristov Svetlin Doshev blaga Rousseva Dimitar Baturov Institute of Space Research and Technology--BAS Aerospace Techniques and Technologies Division Sofia 1113 Bulgaria Military Medical Academy Center of Scientific and Applied Aviation and Naval Medicine and Military Medical Expert&e GeorgiSofiisky Blvd. 3 Sofia 1606 Bulgaria

Complexity of the systems in everyday life of modern man continuously increases, as the monitoring and the management are concentrated on and depended on the reactions of one operator or a group of operators. Sometimes because of human errors in extreme situations, it increases the potential risk for life of large groups of people and of the operators. This requires continuous improvement of the systems for psycho-physiological assessment by developing the new efficient methods involving known and new indicators of psycho-physiological state of the individual. The complex BeOn-1 is a new computer-based experimental and applied system for examination of situational vigilance and behavior of aviation specialists-pilots, navigators, operators of unmanned aerial vehicles in a complex operating environment with extreme impact factors. In the up-to-date systems for evaluating the operators, the test results are compared and analyzed together with a number of physiological parameters that are used as indicators of psycho-physiological status of the investigated subjects. In the ＂BeOn-1＂ they are indicators about the efficiency of individual stress coping strategy. BeOn-I allows us to study the individual skills of perception and the ability to act under extreme conditions of the operational environment and is a comfortable working methodology for daily needs in the selection, periodic monitoring of the operational staff and support to flight safety.

关键词： Modeling of extreme situation assessment of operators skills of perception stress coping strategy

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神殿的基石