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Comparison of antibody detection with <i>Blastomycesdermatitidis</i>yeast lysate antigens in serum specimens from immunized rabbits and infected dogs

作者： (英)rachel Darnley-Smith (英)Helen m. Patey

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Open Journal of Immunology 2011年第3期1卷 74-79页

作者： Will Christenson rachel Horton Kayla Campbell Kelly meacham Amber Schroeder Gene m. Scalarone 不详

This present study was designed to evaluate B. dermatitidis antigens, prepared from two isolates (B5896, 597), when the yeast cells were allowed to lyse in distilled water for one day or seven days. The indirect enzyme-linked immunosorbent assay (ELISA) was used to determine the ability of the lysate reagents to detect antibodies in 30 rabbit and 30 dog serum specimens. mean absorbance values with B5896 lysate antigen ranged from 1.637 (day 1) to 1.461 (day 7) and absorbance values with 597 antigen ranged from 1.579(day 1) to 1.396 (day 7) with the serum specimens from immunized rabbits. Serum specimens from infected dogs yielded absorbance values ranging from 1.672 (day 1) to 1.763 (day 7) with the B5896 and values ranging from 1.909 (day 1) to 1.224 (day 7) with the 597. Optimal reactivity was obtained with the day 1 lysate using both lysate antigens against the rabbit sera and with the 597 antigen against the dog sera. Slightly greater reactivity was evidenced with the day 7 B5896 antigen when the dog sera was tested. Comparative studies are continuing in order to produce an optimal anti-genic preparation for antibody detection in blastomycosis.

关键词： ELISA Blastomycesdermatitidis Antibody Detection Lysate Antigens

Body-focused Anxiety in Women: Associations with Internalization of the Thin-ideal, Dieting Frequency, Body mass Index and media Effects

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Open Journal of medical Psychology 2013年第4期2卷 17-24页

作者： Aileen Pidgeon rachel A. Harker Bond University Australia

Exposure to media that portrays thin women as ideal and attractive can lead to women internalizing the thin ideal, which results in incorporating societal standards of thinness into belief systems. Internalization of the thin-ideal is associated with numerous detrimental effects on women, including decreased levels of self-esteem and increased levels of body-focused anxiety, negative emotions and disordered eating. The present study utilized a sample of women (N = 208) aged between 18 and 67 years (m = 29.44, SD = 13.08) to examine the relationship between internalization of the thin- ideal, body-focused anxiety, body mass index (BmI), and dieting frequency. Correlational, regression and mediation analyses conducted on the data showed that internalization of the thin-ideal, BmI and dieting frequency significantly contributed to body-focused anxiety in women. In addition, body-focused anxiety fully mediated the relationship between internalization of the thin-ideal and dieting frequency among women. BmI did not moderate the relationship between internalization of the thin-ideal and body-focused, indicating that women who internalize the thin-ideal are less vulnerable to dieting unless experiencing body-focused anxiety. The results of the current study enhance our understanding of the relationship between internalization of the thin-ideal, body-focused anxiety, BmI, and dieting frequency among women. Clinical implications will be discussed.

关键词： Body-focused Anxiety Internalization of the Thin-ideal BmI Dieting

Assessment of Genetic Stability Among In Vitro Plants of Arachis retusa Using RAPD and AFLP markers for Germplasm Preservation

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Journal of Integrative Plant Biology 2007年第3期49卷 307-312页

作者： rachel Fatima Gagliardi Luiz Ricardo Hanai Georgia Pacheco Carlos Alberto Oliveira Leonardo Alves Carneiro José Francisco montenegro Valls Elisabeth mansur maria Lucia Carneiro Vieira Laboratório de micropropagacao e Transformacao de Plantas Universidade do Estado do Rio de Janeiro postal code 20550-013 Maracana Rio de Janeiro Brazil Departamento de Genética - Escola Superior de Agricultura Luiz de Queiroz Universidade de Sao Paulo postal code 13418-900 Piracicaba Brazil Embrapa Recursos Genéticos e Biotecnologia postal code 70770-901 Brasilia Brazil

Arachis retusa Krapov. et W. C. Gregory et Valls is endemic in the West-central region of Brazil, occurring In areas endangered by human actions. The establishment of in vitro preservation methods for wild species of Arachis is an alternative to seed banks for germplasm storage, multiplication and distribution. The risk of genetic changes Induced by tissue culture and the monitoring of the genetic stability of the biological material before, during and after storage must be considered In the context of conservation. Random amplified polymorphlc ONA （RAPO） and amplified fragment length polymorphism （AFLP） fingerprinting were used to evaluate the genetic stability of in vitro plants originated from cotyledons and embryo axes of A. retusa. Cotyledons originated shoots through direct organogenesls and embryo axes displayed muItishoot formation Induced by 110 mmol/L and 8.8 mmol/L BAP, respectively. Ninety genomlc regions （loci） generated from RAPO and 372 from AFLP analyses were evaluated. All amplified fragments detected by both techniques in plants derived from the two explant types were monomorphic. The results Indicate that the recovered shoots are genetically stable at the assessed genomic regions.

关键词： amplified fragment length polymorphism Arachis retusa in vitro preservation micropropagation random amplifiedpolymorphic DNA somaclonal variation

methods for extracellular vesicle isolation from cancer cells

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Cancer Drug Resistance 2020年第3期3卷 371-384页

作者： Israa Salem Nicole m.Naranjo Amrita Singh rachel DeRita Shiv Ram Krishn Luca S.Sirman Fabio Quaglia Alexander Duffy Nicholas Bowler Aejaz Sayeed Lucia R.Languino Department of Cancer Biology Sidney Kimmel Cancer CenterThomas Jefferson UniversityPhiladelphiaPA 19107USA. Astellas Institute for Regenerative medicine MarlboroughMA 01752USA. School of Veterinary medicine University of PennsylvaniaPhiladelphiaPA 19104USA. Baruch S.Blumberg Institute DoylestownPA 18902USA.

Cells are known to release different types of vesicles such as small extracellular vesicles(sEVs)and large extracellular vesicles(LEVs).sEVs and LEVs play important roles in intercellular communication,pre-metastatic niche formation,and disease progression;both can be detected cell culture media and biological *** and LEVs contain a variety of protein and RNA cargo,and they are believed to impact many biological functions of the recipient cells upon their internalization or binding to cell surface *** has recently been established that standard isolation techniques,such as differential ultracentrifugation,yield a mixed population of ***,density gradient ultracentrifugation has been reported to allow the isolation of sEVs without cellular ***,we describe the most common methods used to isolate sEVs from cell culture medium,mouse and human plasma,and a new technique for isolating sEVs from tissues as *** article also provides detailed procedures to isolate LEVs.

关键词： Extracellular vesicles differential ultracentrifugation iodixanol density gradient cell culture medium plasma tissue

China Patient-centered Evaluative Assessment of Cardiac Events Prospective Study of Acute myocardial Infarction： Study Design

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Chinese medical Journal 2016年第1期129卷 72-80页

作者： rachel P Dreyer Xi Li Xue Du Nicholas S Downing Li Li Hai-Bo Zhang Fang Feng Wen-Chi Guan Xiao Xu Shu-Xia Li Zhen-Qiu Lin Frederick A masoudi John A Spertus Harlan m Krumholz Li-Xin Jiang National Clinical Research Center of Cardiovascular Diseases State Key Laboratory of Cardiovascular Disease Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100037 China Center for Outcomes Research and Evaluation Yale-New Haven Hospital New Haven Connecticut USA Section of Cardiovascular medicine Department of Internal Medicine Yale School of Medicine New Haven Connecticut USA Department of Obstetrics Gynecology and Reproductive Sciences Yale School of Medicine New Haven Connecticut USA Division of Cardiology University of Colorado Anschutz Medical Campus Aurora Colorado USA Department of Biomedical and Health Informatics University of Missouri - Kansas City Kansas City Missouri USA Saint Luke's mid America Heart Institute Kansas City Missouri USA The Robert Wood Johnson Foundation Clinical Scholars Program Department of Internal Medicine Yale School of Medicine New Haven Connecticut USA Department of Health Policy and management Yale School of Public Health New Haven Connecticut USA

Background： Despite the rapid growth in the incidence of acute myocardial infarction （AmI） in China, there is limited information about patients＇ experiences after AmI hospitalization, especially on long-tern1 adverse events and patient-reported outcomes （PROs）. methods： The China Patient-centered Evaluative Assessment of Cardiac Events （PEACE）-Prospective AmI Study will enroll 4000 consecutive Am I patients from 53 diverse hospitals across China and follow them longitudinally for 12 months to docunlent their treatment, recovery, and outcomes. Details of patients＇ medical history, treatment, and in-hospital outcomes are abstracted from medical charts. Comprehensive baseline interviews are being conducted to characterize patient demographics, risk factors, presentation, and healthcare utilization. As part of these interviews, validated instruments are administered to measure PROs, including quality of life, symptoms, mood, cognition, and sexual activity. Follow-up interviews, measuring PROs, medication adherence, risk factor control, and collecting hospitalization events are conducted at 1, 6, and 12 months after discharge. Supporting documents for potential outcomes are collected for adjudication by clinicians at the National Coordinating Center. Blood and urine samples are also obtained at baseline, 1 - and 12-month follow-up. In addition, we are conducting a survey of participating hospitals to characterize their organizational characteristics. Conclusion： The China PEACE-Prospective AmI study will be uniquely positioned to generate new information regarding patient＇s experiences and outcomes alter AmI in China and seiwe as a foundation for quality improveinent activities.

关键词： Acute myocardial Infarction Outcomes Research Patient-reported Outcome measures Prospective Cohort

Platelet-rich plasma for muscle injuries: A systematic review of the basic science literature

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World Journal of Orthopedics 2019年第7期10卷 278-291页

作者： Kyle N Kunze Charles P Hannon Jared D Fialkoff rachel m Frank Brian J Cole Department of Orthopedic Surgery Rush University Medical CenterChicagoIL 60612United States Department of Orthopedic Surgery University of Colorado School of MedicineBoulderCO 80309United States Department of Orthopedics Rush University Medical CenterChicagoIL 60612United States

BACKGROUND Platelet-rich plasma(PRP) is an increasingly used biologic adjunct for muscle injuries, as it is thought to expedite healing. Despite its widespread use, little is known regarding the mechanisms by which PRP produces its efficacious effects in some *** To clarify the effects of PRP on muscular pathologies at the cellular and tissue levels by evaluating the basic science *** A systematic review of Pubmed/mEDLINE and EmBASE databases was performed using the Preferred Reporting Items for Systematic Reviews and metaAnalyses(PRISmA) guidelines and checklist. Level III in vivo and in vitro studies examining PRP effects on muscles, myocytes and/or myoblasts were eligible for inclusion. Extracted data included PRP preparation methods and study *** Twenty-three studies were included(15 in vivo, 6 in vitro, 2 in vitro/in vivo). Only one reported a complete PRP cytology(platelets, and red and white blood cell counts). Five in vitro studies reported increased cellular proliferation, four reported increased gene expression, and three reported increased cellular differentiation. Five in vivo studies reported increased gene expression, three reported superior muscle regeneration, and seven reported improved histological quality of muscular *** The basic science literature on the use of PRP in muscle pathology demonstrates that PRP treatment confers several potentially beneficial effects on healing in comparison to controls. Future research is needed to determine optimal cytology,dosing, timing, and delivery methods of PRP for muscle pathologies.

关键词： Platelet rich plasma Basic science muscle musculoskeletal Injury

Sodium Diffuses from Glass Substrates through P1 Lines and Passivates Defects in Perovskite Solar modules

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Energy & Environmental materials 2023年第6期6卷 393-401页

作者： Felix Utama Kosasih Francesco Di Giacomo Jordi Ferrer Orri Kexue Li Elizabeth m.Tennyson Weiwei Li Fabio matteocci Gunnar Kusch Narges Yaghoobi Nia rachel A.Oliver Judith L.macmanus-Driscoll Katie L.moore Samuel D.Stranks Aldo Di Carlo Giorgio Divitini Caterina Ducati Department of materials Science and metallurgy University of Cambridge27 Charles Babbage RoadCambridge CB30FSUK Centre for Hybrid and Organic Solar Energy(CHOSE) Department of Electronic EngineeringUniversity of Rome Tor VergataRome 00133Italy Cavendish Laboratory University of CambridgeJJ Thomson AvenueCambridge CB30HEUK Department of materials and Photon Science Institute University of ManchesterOxford RoadManchester M139PLUK Department of Chemical Engineering and Biotechnology University of CambridgePhilippa Fawcett DriveCambridge CB30ASUK Laboratory for Advanced Solar Energy(LASE) National University of Science and Technology MISiSLeninskiy Prospekt 4Moscow 119049Russia Energy Research Institute@NTU Nanyang Technological University50 Nanyang DriveSingapore 637553Singapore mIIT Key Laboratory of Aerospace Information materials and Physics College of PhysicsNanjing University of Aeronautics and AstronauticsNanjing 211106China Istituto di Struttura della materia-CNR(ISm-CNR) Via del Fosso del Cavaliere 100Rome 00133Italy Electron Spectroscopy and Nanoscopy Istituto Italiano di TecnologiaVia Morego 30Genova 16163Italy

most thin-film photovoltaic modules are constructed on soda-lime glass(SLG)substrates containing alkali oxides,such as Na_(2)*** may diffuse from SLG into a module's active layers through P1 lines,an area between a module's constituent cells where the substrate-side charge transport layer(CTL)is in direct contact with *** diffusion from SLG is known to cause several important effects inⅡ-Ⅵand chalcogenide solar modules,but it has not been studied in perovskite solar modules(PSms).In this work,we use complementary microscopy and spectroscopy techniques to show that Na diffusion occurs in the fabrication process of *** diffuses vertically inside P1 lines and then laterally from P1 lines into the active area for up to 360 *** propose that this process is driven by the high temperatures the devices are exposed to during CTL and perovskite *** diffused Na preferentially binds with Br,forming Br-poor,l-rich perovskite and a species rich in Na and Br(Na-Br)close to P1 ***-Br passivates defect sites,reducing non-radiative recombination in the perovskite and boosting its luminescence by up to 5×.Na-Br is observed to be stable after 12 weeks of device storage,suggesting long-lasting effects of Na *** results not only point to a potential avenue to increase PSm performance but also highlight the possibility of unabated Na diffusion throughout a module's lifetime,especially if accelerated by the electric field and elevated temperatures achievable during device operation.

关键词： defect passivation monolithic interconnection perovskite solar modules soda-lime glass sodium diffusion solar cells thin-film photovoltaics

A microliter incubator array for understanding culture condition selectivity

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Advances in Bioscience and Biotechnology 2012年第1期3卷 87-91页

作者： rachel Fulstone Christina m. Coughlan John E. Wiktorowicz Corinne S. Lengsfeld Department Department of Biochemistry & molecular Biology University of Texas Medical Branch Galveston USA Department of Biological Sciences University of Denver Denver USA Department of mechanical and materials Engineering University of Denver Denver USA

The emergence of antibiotic-resistant bacterial strains and the weaponization of rare bacterial strains pose a potential threat of pandemic disease. Those involved in the treatment and control of infectious diseases have called for the development of a device capable of rapidly, simultaneously, and safely investigate a myriad of culture conditions. In response to this need, a microliter incubator array system is described and results from a proof-of-concept study using yeast cells to determine optimal growth conditions is presented.

关键词： microfluidics Infectious Diseases Incubation