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Identific tion of quantitative trait loci and associated candidate genes for pregnancy success in Angus–Brahman crossbred heifers

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Journal of Animal Science and Biotechnology 2024年第1期15卷 162-170页

作者： Quinn A.Hoorn Gabriel A.Zayas Eduardo E.rodriguez Laura m.Jensen raluca G.mateescu Peter J.Hansen Department of Animal Sciences Donald Henry Barron Reproductive and Perinatal Biology Research Programand the Genetics InstituteUniversity of FloridaGainesvilleFLUSA School of Applied Systems Biology La Trobe UniversityBundooraVIC 3083Australia

Background In beef cattle, more than 50% of the energy input to produce a unit of beef is consumed by the female that produced the calf. Development of genomic tools to identify females with high genetic merit for reproductive function could increase the profitability and sustainability of beef *** Genome-wide association studies(GWAS) were performed using a single-step genomic best linear unbiased prediction approach on pregnancy outcome traits from a population of Angus–Brahman crossbred heifers. Furthermore, a validation GWAS was performed using data from another farm. Heifers were genotyped with the Bovine GGP F250 array that contains 221,077 SNPs. In the discovery population, heifers were bred in winter breeding seasons involving a single round of timed artificial insemination(AI) followed by natural mating for 3 months. Two phenotypes were analyzed: pregnancy outcome to first-service AI(PAI;n = 1,481) and pregnancy status at the end of the breeding season(PEBS;n = 1,725). The heritability was estimated as 0.149 and 0.122 for PAI and PEBS, respectively. In the PAI model, one quantitative trait locus(QTL), located between 52.3 and 52.5 mb on BTA7, explained about 3% of the genetic variation, in a region containing a cluster of γ-protocadherin genes and SLC25A2. Other QTLs explaining between 0.5% and 1% of the genetic variation were found on BTA12 and 25. In the PEBS model, a large QTL on BTA7 was synonymous with the QTL for PAI, with minor QTLs located on BTA5, 9, 10, 11, 19, and 20. The validation population for pregnancy status at the end of the breeding season were Angus–Brahman crossbred heifers bred by natural mating. In concordance with the discovery population, the large QTL on BTA7 and QTLs on BTA10 and 12 were *** In summary, QTLs and candidate SNPs identified were associated with pregnancy outcomes in beef heifers, including a large QTL associated with a group of protocadherin genes. Confirmation of these associations

关键词： Beef cattle Fertility GWAS QTL

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rubber band ligation for 750 cases of symptomatic hemorrhoids out of 2200 cases

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World Journal of Gastroenterology 2008年第42期14卷 6525-6530页

作者： Ayman m El Nakeeb Amir A Fikry Waleed H Omar Elyamani m Fouda Tito A El metwally Hosam E Ghazy Sabry A Badr mohmed Y Abu Elkhar Salih m Elawady Hisham H Abd Elmoniam Waiel W Khafagy mosaad m morshed ramadan E El Lithy mohamed E Farid Department of General Surgery Colorectal unit Mansoura University Hospital Mansoura 335111 Egypt

AIm: To study the results for the treatment of symptomatic hemorrhoids using rubber band ligation (rBL) method. mETHODS: A retrospective study for 750 patients who came to the colorectal unit from June, 1998 to September, 2006, data was retrieved from archived fi les. rBL was performed using the mc Gown applicator on an outpatient basis. The patients were asked to return to out-patient clinic for follow up at 2 wk, 1 mo, 6 mo and through telephone call every 6 mo for 2 years). rESULTS: After rBL, 696 patients (92.8%) were cured with no difference in outcome for second or third degree hemorrhoids (P = 0.31). Symptomatic recurrence was detected in 11.04% after 2 years. A total of 52 patients (6.93%) had 77 complications from rBL which required no hospitalization. Complications were pain, rectal bleeding and vaso-vagal symptoms(4.13%, 4.13% and 1.33% of patients, respectively). At 1 mo there were a significant improvement in mean SF-36 scores over baseline in five items, while after 2 years there were improvement in all items over baseline, but not significant. No significant manometeric changes after band ligation. CONCLUSION: rBL is a simple, safe and effective method for treating symptomatic second and third degree hemorrhoids as an out patient procedure with signifi cant improvement in quality of life. rBL doesn't alter ano-rectal functions.

关键词： Piles rectal bleeding barron banding Hemorrhoidectomy

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Elastic band ligation of hemorrhoids:Flexible gastroscope or rigid proctoscope?

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World Journal of Gastroenterology 2007年第4期13卷 585-587页

作者： m Cazemier rJF Felt-Bersma mA Cuesta CJJ mulder Department of Gastroenterology and Hepatology VU University Medical Center Amsterdam The Netherlands Department of Gastrointestinal Surgery VU University Medical Center Amsterdam The Netherlands Department of Gastroenterology and Hepatology VU University Medical Center.Amsterdam The Netherlands

AIm： To compare dgid proctoscope and flexible endoscope for elastic band ligation of internal hemorrhoids. mETHODS： Patients between 18 and 80 years old, with chronic complaints （blood loss, pain, itching or prolapse） of internal hemorrhoids of grade 1-91, were randomized to elastic band ligation by rigid proctoscope or flexible endoscope （preloaded with 7 bands）. Patients were retreated every 6 wk until the cessation of complaints. Evaluation by three-dimensional anal endosonography was performed. rESULTS： Forty-one patients were included （median age 52.0, range 27-79 years, 20 men）. Nineteen patients were treated with a rigid proctoscope and twenty two with a flexible endoscope. Twenty-nine patients had grade I hemorrhoids, 9 patients had grade 11 hemorrhoids and 3 patients had grade 91 hemorrhoids. All patients needed a minimum of 1 treatment and a maximum of 3 treatments. A median of 4.0 bands was used in the rigid proctoscope group and a median of 6.0 bands was used in the flexible endoscope group （P 〈 0.05）. Pain after ligation tended to be more frequent in patients treated with the flexible endoscope （first treatment： 3 vs 20 patients, P 〈 0.05）. Three- dimensional endosonography showed no sphincter defects or alterations in submucosal thickness. CONCLUSION： Both techniques are easy to perform, well tolerated and have a good and fast effect. It is easier to perform more ligations with the flexible endoscope. Additional advantages of the flexible scope are the maneuverability and photographic documentation. However, treatment with the flexible endoscope might be more painful and is more expensive.

关键词： Hemorrhoids barron ligation rigid scope Endoscope Anal endosonography

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对预测教堂内早期声能量衰变的延时耦合空间模型的改进(英文)

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华南理工大学学报（自然科学版） 2007年第B10期35卷 25-27页

作者：褚轶景麦卓明香港理工大学屋宇设备工程系

采用脉冲激励响应的方法对两种不同类型的教堂早期声能量衰变过程进行了研究.结果表明,在barron模型中耦合效应比声源与接收点的距离影响更大.文中还提出了延时耦合空间模型.将几何声学引入统计声学来检测扩散场条件,同时通过对声音从... 详细信息

采用脉冲激励响应的方法对两种不同类型的教堂早期声能量衰变过程进行了研究.结果表明,在barron模型中耦合效应比声源与接收点的距离影响更大.文中还提出了延时耦合空间模型.将几何声学引入统计声学来检测扩散场条件,同时通过对声音从声源传播到每个扩散场子空间的延时,修正了关于脉冲宽度的初始条件.基于耦合空间的理论预测与实际测量结果较为一致.

关键词：早期声能量衰变延时耦合空间模型脉冲激励响应

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使用不同环钻系统的穿透性角膜移植术的长期疗效:来自Halle/Saale大学眼科中心和HeliosAue眼科医院的临床研究

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世界核心医学期刊文摘（眼科学分册） 2005年第5期1卷 43-44页

作者： Jhne U.G. Duncker G.I.W. Jhne m.G. 郭长梅 Augenklinik Helios Kli n. Erfurt Nordhuser Str. 74 99089 Erfurt Germany Dr.

Background: The results of penetrating keratoplasty in two centres for cornea transplantation were investigated with regard to the following parameters: the t rephine system used, the origin of the donor cornea, the influence on the postop erative astigmatism and other aspects. Patients and methods: The retrospective c linical investigation reports about the results of 186 patients with penetrating keratoplasty from the University Eye Hospital of Halle and 204 patients from th e Eye Clinic of the Helios hospital Aue. results: Three trephine systems have be en used: the Hessburg-barron trephine, the guided trephine system (GTS) and the Asmotom device. There were no significant differences between them concerning v isual acuity after 12 months postoperatively. The results were highly significan tly better (P=0.003 Halle; P=0.001 Aue) when using larger diameters of the treph ine (7.5 mm and 8.0 mm). The origin of the donor cornea (“fresh”cornea from a deceased subject or organ cultivated cornea from a cornea bank) had no influence on visual outcome after twelve months (P > 0.8). There were less wound dehiscen ces using the double running suture in comparison with other techniques (P=0.05) . The removal of suturing material was not effective in reducing postoperative a stigmatism (P > 0.11). There was no increase in astigmatism when leaving the sut ures in situ (P > 0.25). Conclusions: Generally, each of the three trephine syst ems can be recommended for clinical use. The trephination should be done with tr ephine diameters of 7.5 mm or 8.0 mm. A timely operation guarantees better visua l acuity at one year postoperatively.

关键词： Halle/Saale HeliosAue 眼科医院眼科中心环钻供体角膜角膜移植缝合技术伤口裂开临床资料

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