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Genome-wide identification and expression analyses of homeodomain-leucine zipper family genes reveal their involvement in stress response in apple(Malus×domestica)

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Horticultural Plant Journal 2022年第3期8卷 261-278页

作者： quanyan zhang Tao Chen Xun Wang Jiahui Wang Kaidi Gu Jianqiang Yu Dagang Hu Yujin Hao National Key Laboratory of Crop Biology Shandong Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTaianShandong 271018China

The homeodomain-leucine zipper(HD-Zip)family has been shown to perform amultitude of functions during plant development and stress responses;however,the familymembers and functions have not been identified in apple(Malus×domestica).In this study,83 HD-Zips(MdHDZs)were identified in the apple *** were assembled into four subgroups according to the classification in Arabidopsis,where MdHDZs in the same subgroup had similar gene structures and conserved protein *** cis-element analysis of MdHDZs promoter regions uncovered numerous elements related to the response of stress and plant *** addition,twelve transcripts of the MdHDZs showed different expression patterns under salt,drought,low temperature and ABA stresses by quantitative reverse transcription-PCR(qRT-PCR)*** further explore the function of MdHDZs in apple,MdHDZ3 was selected to verify its function under salt,low temperature and ABA stresses;and genetic transformation was used to obtain MdHDZ3 transgenic apple *** results demonstrated that MdHDZ3 increased sensitivity to salt,low temperature and abscisic acid in apple calli,suggesting that MdHDZ3 plays an important role in response to *** localization and three-dimensional structural analysis revealed that MdHDZ3 was a nuclear-localized *** together,these findings provide potential information for further identification of HD-Zip proteins in apple.

关键词： Apple HD-Zip HDZ Gene expression Stress response

Genome-Wide Identification, Characterization, and Expression Analysis of Calmodulin-Like Proteins(CMLs) in Apple

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Horticultural Plant Journal 2017年第6期3卷 219-231页

作者： zhang quanyan LIU Xiaojuan LIU Xin WANG Jiahui YU Jianqiang HU Dagang HAO Yujin State Key Laboratory of Crop Biology National Research Center for Apple Engineering and Technology Shandong Agricultural University

Calcium(Ca^(2+)), a crucial second messenger in plants, is involved in diverse signaling pathways including biotic and abiotic stress responses. However, the functions of most calcium sensors including calcium-dependent protein kinases(CDPKs), calmodulins(Ca Ms), calmodulinlike proteins(CMLs), and calcineurin B-like proteins(CBLs) remain limited in plants, particularly in woody plants. Currently, a total of 83 CMLs and seven Ca Ms were discovered in the apple genome in this study. Functional domain analysis showed that these CMLs and Ca Ms contain a highly conserved EF-hand motif. q RT-PCR assays indicated that these CMLs were expressed ubiquitously in apple, including in the roots, stems,leaves, flowers, and fruits, and also possessed spatial specificity. Finally, most of these CMLs were induced by hormonal and abiotic stress, suggesting their potential roles in the regulation of growth, development, and the stress response in apple. In summary, our findings provide potential evidence that apple CMLs may be involved in abiotic stress and the regulation of plant growth and development.

关键词： apple CML abiotic stress plant hormone

Molecular template derived ultrathin N-doped carbon layer on cobalt selenide nanobelts for durable and rapid sodium storage

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Nano Research 2024年第9期17卷 8145-8154页

作者： Chuanliang Wei Baojuan Xi Kangdong Tian Xinlu zhang quanyan Man Keyan Bao Wutao Mao Jinkui Feng Shenglin Xiong Key Laboratory of the Colloid and Interface Chemistry Ministry of EducationSchool of Chemistry and Chemical EngineeringSchool of Materials Science and EngineeringShandong UniversityJinan 250100China Resource Environment and Clean Energy Laboratory School of Chemical and Environmental EngineeringJiangsu University of TechnologyChangzhou 213001China

Sodium-ion batteries(SIBs)are an attractive battery system because of similar characteristics to lithium-ion batteries(LIBs)and large Na element ***,exploring stable,high-capacity and high-rate anode materials for SIBs is still challenging ***,diethylenetriamine(DETA)molecular template derived ultrathin N-doped carbon(NC)layer decorated CoSe_(2)nanobelts(CoSe_(2)/NC)are prepared by solvothermal reaction followed by calcination *** CoSe_(2)/NC exhibits large potential as an anode for *** and theoretical calculations reveal that the in situ formed conductive ultrathin NC layer can not only relieve the volume change of CoSe_(2)but also accelerate electron and ion *** addition,the nanobelt structure of CoSe_(2)/NC with abundant exposed active sites can obviously accelerate the electrochemical *** the synergistic effect of special nanobelt structure and NC layer,the rate as well as cycling performances of CoSe_(2)/NC are obviously improved.A superior capacity retention of 94.8%is achieved at 2 A·g^(-1)after 2000 *** using Na3V2(PO4)3 cathodes,the pouch full batteries can work steadily at 0.5 C,verifying the application ***_(2)/NC anodes also exhibit impressive performances in LIBs and potassium-ion batteries(PIBs).

关键词： CoSe2 nanobelts ultrathin N-doped carbon layer sodium-ion batteries anode high rate

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地铁车站给排水消防设计探讨

给水排水 2007年第12期33卷 88-89页

作者：张泉艳西安市地下铁道有限责任公司西安710016

地铁车站具有建筑结构复杂、环境密闭、人员密集、发生火灾扑救困难等特点,介绍了其消火栓系统的设置要点。因自动喷水灭火系统的灭火、控火率很高,建议国家规范将自动喷水灭火系统引入地铁车站,在地铁车站站厅、站台等主要公共区设置... 详细信息

地铁车站具有建筑结构复杂、环境密闭、人员密集、发生火灾扑救困难等特点,介绍了其消火栓系统的设置要点。因自动喷水灭火系统的灭火、控火率很高,建议国家规范将自动喷水灭火系统引入地铁车站,在地铁车站站厅、站台等主要公共区设置湿式自动喷水灭火系统。

关键词：地铁车站消火栓系统自动喷水灭火系统

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桃果实保鲜技术研究综述

南方农业 2024年第4期18卷 179-181页

作者：翟若涵张全艳陈翠榕高云燕山东省水土保持与环境保育重点实验室山东临沂276000 临沂大学资源环境学院山东临沂276000

桃营养价值丰富,但采后易变质,严重影响桃果实的销售,因此桃果实贮藏保鲜成为桃产业亟待解决的问题。为给桃果实采后保鲜技术的研究提供理论指导,总结桃果实采后生理特性和采后病害,从物理、化学、生物等方面论述桃果实采后保鲜技术的... 详细信息

桃营养价值丰富,但采后易变质,严重影响桃果实的销售,因此桃果实贮藏保鲜成为桃产业亟待解决的问题。为给桃果实采后保鲜技术的研究提供理论指导,总结桃果实采后生理特性和采后病害,从物理、化学、生物等方面论述桃果实采后保鲜技术的研究进展。

关键词：桃生理特性采后病害保鲜技术

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Unraveling a genetic roadmap for improved taste in the domesticated apple

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Molecular Plant 2021年第9期14卷 1454-1471页

作者： Liao Liao Weihan zhang Bo zhang Ting Fang Xiao-Fei Wang Yarning Cai Collins Ogutu Lei Gao Gang Chen Xiaoqing Nie Jinsheng Xu quanyan zhang Yiran Ren Jianqiang Yu Chukun Wang Cecilia H.Deng Baiquan Ma Beibei Zheng Chun-Xiang You Da-Gang Hu Richard Espley Kui Lin-Wang Jia-Long Yao Andrew C.Allan Awais Khan Schuyler S.Korban zhangjun Fei Ray Ming Yu-Jin Hao Li Li Yuepeng Han CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Hubei Hongshan LaboratoryWuhan Botanical GardenThe Innovative Academy of Seed DesignChinese Academy of SciencesWuhan 430074China Agricultural Bioinformatics Key Laboratory of Hubei Province College of InformaticsHuazhong Agricultural UniversityWuhan 430070China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesWuhan 430074China University of Chinese Academy of Sciences 19A YuquanluBeijing 100049China College of Horticulture Science and Engineering Shandong Agricultural UniversityTai-An 271018China Sino-African Joint Research Center Chinese Academy of SciencesWuhan 430074China The New Zealand Institute for Plant&Food Research Limited AucklandNew Zealand School of Biological Sciences University of AucklandPrivate Bag 92019Auckland 1142New Zealand Plant Pathology and Plant-Microbe Biology Section Cornell UniversityGenevaNY 14456USA Department of Natural Resources and Environmental Sciences University of Illinois at Urbana-ChampaignUrbanaIL 61801USA Boyce Thompson Institute Cornell UniversityIthacaNY 14853USA Department of Plant Biology University of Illinois at Urbana-ChampaignUrbanaIL 61801USA

Although taste is an important aspect of fruit quality, an understanding of its genetic control remains elusive in apple and other fruit crops. In this study, we conducted genomic sequence analysis of 497 Malus accessions and revealed erosion of genetic diversity caused by apple breeding and possible independent domestication events of dessert and cider apples. Signatures of selection for fruit acidity and size, but not for fruit sugar content, were detected during the processes of both domestication and improvement. Furthermore, we found that single mutations in major genes affecting fruit taste, including Ma1, MdTDT, and MdSOT2, dramatically decrease malate, citrate, and sorbitol accumulation, respectively, and correspond to important domestication events. Interestingly, Ma1 was identified to have pleiotropic effects on both organic acid content and sugar:acid ratio, suggesting that it plays a vital role in determining fruit taste. Fruit taste is unlikely to have been negatively affected by linkage drag associated with selection for larger fruit that resulted from the pyramiding of multiple genes with minor effects on fruit size. Collectively, our study provides new insights into the genetic basis of fruit quality and its evolutionary roadmap during apple domestication, pinpointing several candidate genes for genetic manipulation of fruit taste in apple.

关键词： apple fruit taste organic acids soluble sugars fruit size domestication

苹果MdNAC143的克隆及其在苹果愈伤组织的抗盐功能鉴定

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园艺学报 2017年第11期44卷 2163-2170页

作者：张全艳于建强王佳慧胡大刚郝玉金山东农业大学园艺科学与工程学院作物生物学国家重点实验室农业部黄淮地区园艺作物生物学与种质创制重点实验室山东泰安271018

从‘嘎拉’苹果中克隆了一个NAC转录因子基因MdNAC143(序列号:MDP0000334047),其开放阅读框为924 bp,编码308个氨基酸,预测蛋白质的分子量为35.59 k D,等电点为6.32。结构域分析表明,MdNAC143蛋白N端含有保守的NAC结构域。酵母双杂交结... 详细信息

从‘嘎拉’苹果中克隆了一个NAC转录因子基因MdNAC143(序列号:MDP0000334047),其开放阅读框为924 bp,编码308个氨基酸,预测蛋白质的分子量为35.59 k D,等电点为6.32。结构域分析表明,MdNAC143蛋白N端含有保守的NAC结构域。酵母双杂交结果表明MdNAC143的全长及C端具有转录激活活性。荧光定量PCR分析表明,MdNAC143在苹果的各个组织均有表达,其中在叶片和花中表达相对较高;MdNAC143的表达明显受盐胁迫的诱导。将MdNAC143遗传转化‘王林’苹果愈伤组织,进行抗盐表型鉴定后发现,MdNAC143在愈伤组织中过量表达后能明显提高对盐胁迫的抗性。

关键词：苹果 MdNAC143 基因表达转录激活盐胁迫

基于六个高代回交群体玉米叶形结构相关性状的杂种优势研究

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种子 2022年第7期41卷 9-15页

作者：张全艳杨得正张培高徐春霞王晶刘丽云南大学农学院昆明650504 云南省农业科学院粮食作物研究所昆明650205 云南省农业科学院生物技术与种质资源研究所昆明650205

本研究基于项目组前期构建的三角形杂种优势新模式,从Suwan 1群、Reid群和非Reid群中选择3个最具有代表性的自交系Y 46、Y 107和MON 2,构建了六个高代回交群体,分析各群体叶长、叶宽和叶夹角等叶形结构相关性状的遗传差异及杂种优势。... 详细信息

本研究基于项目组前期构建的三角形杂种优势新模式,从Suwan 1群、Reid群和非Reid群中选择3个最具有代表性的自交系Y 46、Y 107和MON 2,构建了六个高代回交群体,分析各群体叶长、叶宽和叶夹角等叶形结构相关性状的遗传差异及杂种优势。结果表明,六个高代回交群体的叶长、叶宽及叶夹角均存在显著差异,其中Y 46与Y 107组配的高代回交群体叶形结构相关性状均显著高于Y 46与MON 2和Y 107与MON 2组配的群体,且Y 46与MON 2组配的正反交群体间各性状差异均较大。Y 107与MON 2组配的高代回交群体其叶形结构相关性状的杂种优势较强,Y 46与MON 2群体杂种优势较弱,Y 46与Y 107群体杂种优势介于二者之间。在三角形杂种优势模式中,Reid×非Reid杂种优势较强,Suwan 1×Reid杂种优势次之,Suwan 1×非Reid杂种优势较弱。研究表明,Reid×非Reid是培育理想株型新品种的重要杂种优势模式,建议在高密度品种选育中优先选用。

关键词：玉米高代回交群体叶形结构相关性状杂种优势

苹果乙烯响应因子基因MdERF11对脱落酸的敏感性分析

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园艺学报 2020年第1期47卷 1-10页

作者：王佳慧于建强张全艳韩朋良由春香胡大刚郝玉金山东农业大学园艺科学与工程学院作物生物学国家重点实验室农业部黄淮地区园艺作物生物学与种质创制重点实验室山东泰安271018

以‘嘎拉’苹果(Malus×domestica Borkh.)为试材,克隆了乙烯响应因子基因MdERF11(序列号MDP0000756341)。测序发现,该基因包含全长为483 bp的完整开放阅读框,编码161个氨基酸。系统进化树分析表明,这一乙烯响应因子与拟南芥AtERF11蛋... 详细信息

以‘嘎拉’苹果(Malus×domestica Borkh.)为试材,克隆了乙烯响应因子基因MdERF11(序列号MDP0000756341)。测序发现,该基因包含全长为483 bp的完整开放阅读框,编码161个氨基酸。系统进化树分析表明,这一乙烯响应因子与拟南芥AtERF11蛋白同源序列相似性最高。利用PlantCare数据库进行基因启动子顺式作用元件预测,MdERF11启动子序列中含有与脱落酸(ABA)、乙烯及干旱信号相关的顺式作用元件。荧光定量PCR分析表明,MdERF11在苹果的各组织中均有表达,在叶柄和果实中表达量相对较高;并且MdERF11的表达明显受到ABA的诱导。在外源ABA的处理下,MdERF11过量表达的苹果愈伤组织的生长势明显比野生型强,表明MdERF11降低了苹果愈伤组织对ABA的敏感性。

关键词：苹果 MdERF11 乙烯响应因子脱落酸