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Water utilization of typical plant communities in desert steppe,China

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Journal of Arid Land 2022年第9期14卷 1038-1054页

作者： CHEN Juan wang Xing SONG Naiping wang qixue WU Xudong School of Agriculture Ningxia UniversityYinchuan 750021China Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in northwestern China Yinchuan 750021China Key Laboratory of Restoration and Reconstruction of Degraded Ecosystems in northwestern China of Ministry of Education Yinchuan 750021China School of Ecology and Environment Ningxia UniversityYinchuan 750021China Institute of Desertification Control Ningxia Academy of Agricultural and Forestry SciencesYinchuan 750002China

Water is a limiting factor in the restoration and construction of desert *** plant water sources is necessary to understand soil-plant interactions and species coexistence;however,water sources of major plant communities within the desert steppe of Ningxia Hui Autonomous Region,China remain poorly *** this study,we analyzed the water uptake of plants in four typical communities:Agropyron mongolicum Keng.;Sophora alopecuroids Linn.;Stipa breviflora Griseb.,and Achnatherum splendens(Trin.)Nevski *** isotopesδD andδ^(18)O in the xylem of plant and soil water at different soil depths were *** IsoSource model was used to determine the soil depths from which plants obtained *** showed that *** community obtained water predominantly from 0–20 and 40–80 cm depth,*** community from 0–20 cm depth,*** community from 0–40 cm depth,and *** community from 0–20 and 80–140 cm *** had a wider range of soil depths for water extraction,i.e.,utilizing different water sources depending on habitat,and the plasticity of its water uptake pattern determined its role in different *** source of plants relayed heavily on the distribution of their *** for soil water exists between different plant life forms in the sierozem habitat(***,***,and *** communities),and in the sandy soil habitat(*** community).The use of soil water by *** community is more spatially differentiated,and shrubs and herbs can coexist *** the pattern of extended drought period in the future,sierozem habitat may be more favorable for the formation of a dominant monoculture community type of perennial fibrous *** aeolian sandy soil habitat,*** had a strong competitive advantage,and the growth of shallow-rooted plants was easily suppressed.

关键词： stable isotope water source IsoSource model soil water desert steppe

Identification of the E2F1-RAD51AP1 axis as a key factor in MGMT-methylated GBM TMZ resistance

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Cancer Biology & Medicine 2023年第5期20卷 385-400页

作者： Junhu Zhou Fei Tong Jixing Zhao Xiaoteng Cui Yunfei wang Guangxiu wang Chunsheng Kang Xiaomin Liu qixue wang Tianjin Medical University General Hospital Tianjin 300052China Tianjin Neurological Institute Key Laboratory of NeurotraumaVariationand RegenerationMinistry of Education and Tianjin Municipal GovernmentTianjin 300052China Department of Oncology Gamma Knife CenterDepartment of Neurological SurgeryTianjin Huanhu HospitalNankai UniversityTianjin 300350China

Objective:Epidermal growth factor receptor variant III(EGFRvIII)is a constitutively-activated mutation of EGFR that contributes to the malignant progression of glioblastoma multiforme(GBM).Temozolomide(TMZ)is a standard chemotherapeutic for GBM,but TMZ treatment benefits are compromised by *** study aimed to elucidate the crucial mechanisms leading to EGFRvIII and TMZ ***:CRISPR-Cas13a single-cell RNA-seq was performed to thoroughly mine EGFRvIII function in *** blot,realtime PCR,flow cytometry,and immunofluorescence were used to determine the chemoresistance role of E2F1 and RAD51-associated protein 1(RAD51AP1).Results:Bioinformatic analysis identified E2F1 as the key transcription factor in EGFRvIII-positive living *** RNA-seq analysis revealed that E2F1 is a crucial transcription factor under TMZ *** blot suggested enhanced expression of E2F1 in EGFRvIII-positive and TMZ-treated glioma *** of E2F1 increased sensitivity to *** diagram profiling showed that RAD51AP1 is positively correlated with E2F1,mediates TMZ resistance,and has a potential E2F1 binding site on the *** of RAD51AP1 enhanced the sensitivity of TMZ;however,overexpression of RAD51AP1 was not sufficient to cause chemotherapy resistance in glioma ***,RAD51AP1 did not impact TMZ sensitivity in GBM cells with high O6-methylguanine-DNA methyltransferase(MGMT)*** level of RAD51AP1 expression correlated with the survival rate in MGMT-methylated,but not MGMT-unmethylated TMZ-treated GBM ***:Our results suggest that E2F1 is a key transcription factor in EGFRvIII-positive glioma cells and quickly responds to TMZ ***51AP1 was shown to be upregulated by E2F1 for DNA double strand break *** RAD51AP1 could facilitate achieving an ideal therapeutic effect in MGMT-methylated GBM cells.

关键词： TMZ EGFRvIII E2F1 RAD51AP1 MGMT

Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma

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Cancer Communications 2023年第12期43卷 1326-1353页

作者： Xiaoteng Cui Jixing Zhao Guanzhang Li Chao Yang Shixue Yang Qi Zhan Junhu Zhou Yunfei wang Menglin Xiao Biao Hong Kaikai Yi Fei Tong Yanli Tan Hu wang qixue wang Tao Jiang Chuan Fang Chunsheng Kang Laboratory of Neuro-oncology Tianjin Neurological Institute Tianjin Medical University General Hospital Tianjin P. R. China Key Laboratory of Post-Neuro Injury Neuro-repair and Regeneration in Central Nervous System Ministry of Education and Tianjin City Tianjin P. R. China Department of Neurosurgery Beijing Tiantan Hospital Capital Medical University Beijing P. R. China Chinese Glioma Genome Atlas Network (CGGA) and Asian Glioma Genome Atlas Network (AGGA) Beijing P. R. China Department of Neurosurgery The First Affiliated Hospital of Zhengzhou University Zhengzhou Henan P. R. China Tianjin Key Laboratory of Composite and Functional Materials School of Material Science and Engineering Tianjin University Tianjin P. R. China Department of Neurosurgery Affiliated Hospital of Hebei University Baoding Hebei P. R. China Hebei Key Laboratory of Precise Diagnosis and Treatment of Glioma Baoding Hebei P. R. China Department of Neuro-Oncology and Neurosurgery Tianjin Medical University Cancer Institute and Hospital Tianjin P. R. China Department of Pathology Affiliated Hospital of Hebei University Baoding Hebei P. R. China Department of Pathology Hebei University School of Basic Medical Sciences Baoding Hebei P. R. China Department of Neurosurgery Tianjin Huanhu Hospital Tianjin P. R. China Department of Molecular Neuropathology Beijing Neurosurgical Institute Capital Medical University Beijing P. R. China Research Unit of Accurate Diagnosis Treatment and Translational Medicine of Brain Tumors Chinese Academy of Medical Sciences Beijing P. R. China

Background Metabolism reprogramming plays a vital role in glioblastoma(GBM)progression and recurrence by producing enough energy for highly proliferating tumor *** addition,metabolic reprogramming is crucial for tumor growth and immune-escape *** growth factor receptor(EGFR)amplification and EGFR-vIII mutation are often detected in GBM cells,contributing to the malignant *** study aimed to investigate the functional role of the EGFR pathway on fatty acid metabolism remodeling and energy *** Clinical GBM specimens were selected for single-cell RNA sequencing and untargeted metabolomics analysis.A metabolism-associated RTK-fatty acid-gene signature was constructed and ***-2206 and MK-803 were utilized to block the RTK pathway and mevalonate pathway induced abnormal *** metabolism in GBM with activated EGFR pathway was *** antitumor effect of Osimertinib and Atorvastatin assisted by temozolomide(TMZ)was analyzed by an intracranial tumor model in *** GBM with high EGFR expression had characteristics of lipid remodeling and maintaining high cholesterol levels,supported by the single-cell RNA sequencing and metabolomics of clinical GBM *** of the EGFR/AKT and mevalonate pathways could remodel energy metabolism by repressing the tricarboxylic acid cycle and modulating ATP ***,the EGFR/AKT pathway upregulated the expressions of acyl-CoA synthetase short-chain family member 3(ACSS3),acyl-CoA synthetase long-chain family member 3(ACSL3),and long-chain fatty acid elongation-related gene ELOVL fatty acid elongase 2(ELOVL2)in an NF-κB-dependent ***,inhibition of the mevalonate pathway reduced the EGFR level on the cell membranes,thereby affecting the signal transduction of the EGFR/AKT ***,targeting the EGFR/AKT and mevalonate pathways enhanced the antitumor effect of TMZ in GBM cells and animal *** Our findings no

关键词： combinatorial therapeutic strategy EGFR energy metabolism glioblastoma

TGFβ signaling-induced miRNA participates in autophagic regulation by targeting PRAS40 in mesenchymal subtype of glioblastoma

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Cancer Biology & Medicine 2020年第3期17卷 664-675页

作者： Yingbin Xie Luyue Chen Junhu Zhou qixue wang Chao Yang Can Xu Xiangyu Fan Yanli Tan Yanan wang Chunsheng Kang Chuan Fang Department of Neurosurgery Hebei University Affiliated HospitalBaoding 071000China Laboratory of Neuro-0neology Tianjin Neurological InstituteDepartment of NeurosurgeryTianjin Medical University General Hospital and Key Laboratory of NeurotraumaVariationand RegenerationMinistry of Education and Tianjin Municipal GovernmentTianjin 300052China Department of Neurosurgery Zhongshan Hospital Xiamen UniversityXiamen 361004China Department of Pathology Hebei University Affiliated HospitalBaoding 071000China Affiliated Cancer Hospital and Institute of Guangzhou Medical University Guangzhou 510095China

Objective:Mesenchymal subtype of glioblastoma(mesGBM)is a refractory disease condition characterized by therapeutic failure and tumor *** transforming growth factor-β(TGF-β)signaling could be a signature event in mesGBM,which leads to dysregulation of downstream targets and contribute to malignant *** this study we aimed to investigate the hyperactive TGFβsignaling-mediated pathogenesis and possible downstream targets for the development of novel therapeutic interventions for ***:GBM-BioDP is an online resource for accessing and displaying interactive views of the TCGA GBM data *** sequencing followed by bioinformatic analysis was performed to identify dysregulated *** prediction by MR-microT and dual luciferase reporter assay were utilized to confirm the predicted target of novel_***-8 assays was used to assesse cell *** miRNA manipulation was proceeded by cell transfection and lentivirus delivery.A plasmid expressing GFP-LC3 was introduced to visualize the formation of *** GBM model was constructed forin vivo ***:TGFβ1 and TGFβreceptor type II(TβRII)were exclusively upregulated in mesGBM(P<0.01).Dysregulated miRNAs were identified after LY2109761(a TβRI/II inhibitor)treatment in a mesGBM-derived cell line,and novel_miR56 was selected as a promising candidate for further functional ***_miR56 was found to potentially bind to PRAS40 via seed region complementarity in the 3'untranslated region,and we also confirmed that PRAS40 is a direct target of novel_miR56 in glioma *** vitro,over expression of novel_miR56 in tumor cells significantly promoted proliferation and inhibited autophagy(P<0.05).The expression levels of P62/SQSTM was significantly increased accompanied by the decrease of BECN1 and LC3B-II/I,which indicated that autophagic activity was reduced after novel_miR56 *** addition,over expression of novel_miR56 also

关键词： Autophagy glioblastoma microRNA PRAS40 TGFβsignaling

荒漠区湿地植物群落分类排序及其与土壤环境因子的关系

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草业科学 2023年第1期40卷 37-45页

作者：王启学王兴余轩陈娟宋乃平吴旭东宁夏大学生态环境学院宁夏银川750021 宁夏大学西北退化生态环境恢复与重建教育部重点实验室宁夏银川750021 宁夏大学西北土地退化与生态系统恢复国家重点实验室培养基地宁夏银川750021 宁夏大学农学院宁夏银川750021 宁夏农林科学院荒漠化防治研究所宁夏银川750002

湿地存亡可以作为荒漠地区植被恢复可持续性的指示器,明晰其生态系统内部植物群落空间分布特征及其与土壤环境因子关系,可为制定与生境高度相适应的多样化植被恢复模式提供科学依据。本研究以宁夏哈巴湖湿地汇水范围为研究对象,在12 km... 详细信息

湿地存亡可以作为荒漠地区植被恢复可持续性的指示器,明晰其生态系统内部植物群落空间分布特征及其与土壤环境因子关系,可为制定与生境高度相适应的多样化植被恢复模式提供科学依据。本研究以宁夏哈巴湖湿地汇水范围为研究对象,在12 km×12 km范围内,设置分辨率为1 km×1 km的152个调查样点的植被和土壤数据,采用多元回归树和冗余分析,探讨不同植物群落类型的空间分布特征及其对土壤理化因子的响应规律。结果表明:1)研究区植物群落可划分为短花针茅(Stipa breviflora)+牛枝子(Lespedeza bicolor)、冰草(Agropyron mongolicum)+赖草(Leymus secalinus)+草木樨状黄芪(Astragalus melilotoides)、苦豆子(Sophora alopecuroide)+猪毛蒿(Artemisia scoparia)、芨芨草(Splendid achnatherum)+盐爪爪(Slenderbranch Kalidium) 4种植物群落类型。2)土壤粘粉粒含量、生境海拔高度、土壤pH和盐分含量是4种植物群落空间分布的主要控制因素。3)随着土壤粘粉粒或细沙粒含量降低所表征的土壤粗质化程度增加,植物群落由短花针茅+牛枝子转变为冰草+赖草+草木樨状黄芪;随着海拔降低和土壤全盐增加,植物群落由短花针茅+牛枝子转变为芨芨草+盐爪爪。综上可知,荒漠湿地由分水岭高梁地到低滩湿地的景观植被系统,首先是土壤质地决定的旱生地带性植物群落空间分异,其次是海拔和盐分驱动的地带性向隐域性植物群落的二次分异。

关键词：群落分类排序多元回归树冗余分析植物群落空间分异主控因素哈巴湖湿地植被恢复

橄榄苦苷调节Notch/Hes-1信号通路对HPV阳性宫颈癌细胞活力与凋亡的影响

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中国病原生物学杂志 2024年第7期19卷 779-783页

作者：刘晓彤魏茜雪王兰中国医科大学附属医院盛京医院妇科肿瘤病房辽宁沈阳110000

目的探究橄榄苦苷(OLE)对人乳头状瘤病毒(HPV)阳性宫颈癌细胞活力与凋亡的影响及其对Notch/Hes-1信号通路的调控机制。方法将人宫颈癌细胞HeLa229随机分为HeLa229组、OLE低浓度组(OLE-L组)、OL中浓度组(OLE-M组)、OL高浓度组(OLE-H组)... 详细信息

目的探究橄榄苦苷(OLE)对人乳头状瘤病毒(HPV)阳性宫颈癌细胞活力与凋亡的影响及其对Notch/Hes-1信号通路的调控机制。方法将人宫颈癌细胞HeLa229随机分为HeLa229组、OLE低浓度组(OLE-L组)、OL中浓度组(OLE-M组)、OL高浓度组(OLE-H组)和OL-H+Notch激活剂(Jagged1)组(OLE-H+Jagged1组);采用CCK-8法和克隆平板检测各组细胞的存活率及克隆数量;采用Transwell实验检测各组细胞的迁移情况;采用MTT比色法测定各组细胞活力;采用流式细胞术检测各组细胞的凋亡情况;采用蛋白免疫印迹(Western blot)检测各组细胞中Notch/Hes-1信号通路相关蛋白的表达水平。结果与HeLa229组相比,OLE-L组、OLE-M组和OLE-H组细胞存活率、克隆数量、细胞迁移数量、细胞活力以及细胞中Notch、Hes-1蛋白表达水平均降低(P<0.05),而细胞凋亡率升高(P<0.05);Jagged1的加入逆转了高浓度OLE对HeLa229细胞增殖、凋亡等指标的影响,且细胞中Notch、Hes-1蛋白表达水平升高(P<0.05)。结论 OLE能够抑制HPV阳性宫颈癌细胞活力,促进其凋亡,这可能与Notch/Hes-1信号通路被抑制有关。

关键词：橄榄苦苷 Notch/Hes-1信号通路 HPV阳性宫颈癌

The Role of Liver X Receptor in Anti-tumorigenesis

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The Role of Liver X Receptor in Anti-tumorigenesis

胶质母细胞瘤中的分子标记物和靶向治疗的研究进展

脂质组学与脂蛋白的新功能——第十二届全国脂质与脂蛋白学术会议

作者： Yajun Duan qixue wang Xingzhe Ma Yuanli Chen Ying Liu Jihong Han State Key Laboratory of Medicinal Chemical Biology Nankai University

Background and purpose:Liver X receptor(LXR)is a ligand-activated transcription factor and plays an important role in regulation of lipid homoeostasis and ***γ(IFN-γ)has multiple functions including the protection of organisms against infection from viruses,bacteria and parasites,and *** a few studies have indicated that LXR inhibits IFN-y-induced biological responses;the influence of LXR

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中华神经医学杂志 2019年第6期18卷 629-633页

作者：王琦雪赵凯康春生天津医科大学总医院神经病学研究所天津300052

多形性胶质母细胞瘤是最常见的中枢神经系统侵袭性恶性肿瘤。胶质母细胞瘤的分子标志物是建立在庞大数据库的基础上,通过大量的研究和算法得到的特异性存在于胶质母细胞瘤中,并且能够反映胶质母细胞瘤发生、发展或者监测其对治疗反应的... 详细信息

多形性胶质母细胞瘤是最常见的中枢神经系统侵袭性恶性肿瘤。胶质母细胞瘤的分子标志物是建立在庞大数据库的基础上,通过大量的研究和算法得到的特异性存在于胶质母细胞瘤中,并且能够反映胶质母细胞瘤发生、发展或者监测其对治疗反应的一类物质。对胶质母细胞瘤分子标志物的筛选和鉴定能够有效区分肿瘤亚型、恶性程度和放化疗的响应性。本文归纳总结胶质母细胞瘤的分子标记物,并且探讨针对它们的靶向治疗的前景。

关键词：胶质母细胞瘤分子标志物靶向治疗

THE STUDY OF RELATIVITY BETWEEN CHLORIDE CHANNELS AND PROLIFERATIVE VITREORETINOPATHY FORMATION

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THE STUDY OF RELATIVITY BETWEEN CHLORIDE CHANNELS AND PROLIF...