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Deletion of a 1,049 bp sequence from the 5′UTR upstream of the SiHEC3 gene induces a seed non-shattering mutation in sesame

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Journal of Integrative Agriculture 2024年第8期23卷 2589-2604页

作者： Ming Ju Guiting Li qiuzhen tian Hengchun Cao Qin Ma Yinghui Duan Hui Guo Zhanyou Zhang Yingying Huang Huili Wang Haiyang Zhang Hongmei Miao The Shennong Laboratory Zhengzhou 450002China Henan Sesame Research Center Henan Academy of Agricultural SciencesZhengzhou 450002China Key Laboratory of Specific Oilseed Crops Genomics of Henan Province Henan Sesame Research CenterHenan Academy of Agricultural SciencesZhengzhou 450002China

Sesame is a labor intensive crop with limited mechanized harvesting mainly due to the seed shattering(SS)*** this study,we performed a genetic analysis of the seed-shattering resistance(SR)trait with a SR sesame mutant *** the SS type,the parenchyma cells in the abscission zone of the 12M07 mutant are arranged loosely but adhere to the seed *** analysis of six generations derived from 12M07(SR)×Xiangcheng Dazibai(SS)showed that the SR trait is recessive and controlled by a single gene *** mapping of the F2population with 888,619 variants(single-nucleotide polymorphisms(SNPs)and insertion-deletion(InDels))and 31,884 structural variations(SVs)determined that only SV12002 in the 5′upstream region of gene Sindi0765000(named SiHEC3)in Chr.3 was significantly associated with the SR ***3 encodes the bHLH transcription factor.A 1,049 bp deletion occurred in the 5′UTR of Sihec3 in ***3 is mainly expressed in developing placental tissues,with the expression peaking in capsules at 45 days after pollination.A dual-luciferase reporter assay in tobacco confirmed that the promoter activity of Sihec3 was reduced because of the deletion of the 1,049 bp promoter ***–protein interaction network analysis showed that HEC3 is co-expressed with nine key proteins,such as SHATTERPROOF1(SHP1)and SEEDSTICK(STK)which participate in the secondary wall biosynthesis of the abscission layer in *** findings of this study show the important function of Sihec3corresponding with the SR trait and supply the genetic information for breeding new varieties that are amenable to mechanized harvesting in sesame and other crops.

关键词： sesame(Sesamum indicum L.) association mapping seed shattering structure variation bHLH transcription factor

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Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L.

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The Crop Journal 2024年第1期12卷 252-261页

作者： Yinghui Duan Wenwen Qu Shuxian Chang Ming Ju Cuiying Wang Cong Mu Hengchun Cao Guiting Li qiuzhen tian Qin Ma Zhanyou Zhang Haiyang Zhang Hongmei Miao The Shennong Laboratory Zhengzhou 450002HenanChina Henan Sesame Research Center Henan Academy of Agricultural SciencesZhengzhou 450002HenanChina Key Laboratory of Specific Oilseed Crops Genomics of Henan Province Henan Sesame Research CenterHenan Academy of Agricultural SciencesZhengzhou 450002HenanChina

Sesame Fusarium wilt(SFW),caused by Fusarium oxysporum ***(Fos),is one of the most devastating diseases affecting sesame *** the genetic control of SFW resistance is pivotal for effective disease management in *** inheritance study on a cross between the highly resistant variety Yuzhi 11 and the highly susceptible accession Sp1 using a Fos pathogenicity group 1 isolate indicated that resistance was conferred by a single dominant *** target locus was located in a 1.24 Mb interval on chromosome 3 using a combination of cross-population association mapping and bulked segregant *** genetic mapping further narrowed the interval between 21,350 and 21,401 *** locus Sindi_0812400 was identified as the SFW resistance gene and officially designated *** gene encodes a specific malectin/receptor-like protein kinase with three putative tandem kinase domains and is considered a kinase fusion *** analysis revealed that a high proportion(49.44%)of variants within the locus was located within the kinase domainⅢ,and several of which were evidently associated with the diversity in SFW response,indicating the critical role of kinase domainⅢin expression of disease *** findings provide valuable information for further functional analysis of SFW resistance genes and marker-assisted resistance breeding in sesame.

关键词： Breeding for resistance Genetic analysis Molecular analysis Tandem kinase domain

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Genomic evolution and insights into agronomic trait innovations of Sesamum species

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Plant Communications 2024年第1期5卷 262-283页

作者： Hongmei Miao Lei Wang Lingbo Qu Hongyan Liu Yamin Sun Meiwang Le Qiang Wang Shuangling Wei Yongzhan Zheng Wenchao Lin Yinghui Duan Hengchun Cao Songjin Xiong Xuede Wang Libin Wei Chun Li Qin Ma Ming Ju Ruihong Zhao Guiting Li Cong Mu qiuzhen tian Hongxian Mei Tide Zhang Tongmei Gao Haiyang Zhang Henan Sesame Research Center Henan Academy of Agricultural SciencesZhengzhou 450002China TEDA School of Biological Sciences and Biotechnology Nankai UniversityTianjin 300457China College of Food Science and Technology Henan Technology UniversityZhengzhou 450001China Institute of Plant Protection Research Henan Academy of Agricultural SciencesZhengzhou 450002China Crops Research Institute Jiangxi Academy of Agricultural SciencesNanchang 330200China Crop Research Institute Anhui Academy of Agricultural SciencesHefei 230031China

Sesame is an ancient oilseed crop with high oil content and ***,the evolutionary history and genetic mechanisms of its valuable agronomic traits remain ***,we report chromosome-scale genomes of cultivated sesame(Sesamum indicum L.)and six wild Sesamum species,representing all three karyotypes within this *** and genome-based phylogenic analysis revealed the evolutionary route of Sesamum species from n=13 to n=16 and revealed that allotetraploidization occurred in the wild species Sesamum *** divergence of the Sesamum genus(48.5–19.7 million years ago)during the Tertiary period and its ancient phylogenic position within eudicots were ***-genome analysis revealed 9164 core gene families in the *** families are significantly enriched in variousmetabolic pathways,including fatty acid(FA)metabolism and FA *** variations in SiPT1 and SiDT1 within the phosphatidyl ethanolamine-binding protein gene family lead to the genomic evolution of plant-architecture and inflorescence-development phenotypes in Sesamum.A genome-wide association study(GWAS)of an interspecific population and genome comparisons revealed a long terminal repeat insertion and a sequence deletion inDIR genes of wildSesamum angustifoliumand cultivated sesame,respectively;both variations independently cause high susceptibility toFusariumwilt disease.A GWAS of 560 sesame accessions combined with an overexpression study confirmed that the NAC1andPPOgenes play an important role in upregulating oil content of *** study provides high-quality genomic resources for cultivated and wild Sesamum species and insights that can improve molecular breeding strategies for sesame and other oilseed crops.

关键词： Sesamum genome evolution structural variation plant architecture Fusarium wilt disease oil content

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LOWER TEMPERATURE 1 Enhances ABA Responses and Plant Drought Tolerance by Modulating the Stability and Localization of C2-Domain ABA-Related Proteins in Arabidopsis

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Molecular Plant 2019年第9期12卷 1243-1258页

作者： Tao Qin qiuzhen tian Guifeng Wang Liming Xiong College of Grassland Agriculture Northwest A&F UniversityYangling 712100China Key Laboratory of Wheat and Maize Crops Science Collaborative Innovation Center of Henan Grain CropsCollege of AgronomyHenan Agricultural UniversityZhengzhou 450002China Department of Biology Hong Kong Baptist UniversityKowloon TongHong KongChina Texas A&M Agrilife Research Center DallasTXUSA State Key Laboratory of Agrobiotechnology Chinese University of Hong KongMa Liu ShuiHong KongChina

Plasma membrane-associated abscisic acid(ABA)signal transduction is an integral part of ABA *** C2-domain ABA-related(CAR)proteins play important roles in the recruitment of ABA receptors to the plasma membrane to facilitate ABA ***,how CAR proteins are regulated remains *** this study,we conducted a genetic screen for mutants with altered leaf transpiration and identified an uncharacterized protein,LOWER TEMPERATURE 1(LOT1),which regulates the dynamic localization and stability of CAR *** lotimutant had a lower leaf temperature as compared with the wild type due to higher *** found that LOT1 physically interacts with CAR9,and ABA reduces LOT1-CAR9 interaction in the nucleus,likely via Ca^2+,resulting in increased localization of CAR9 to the plasma *** further found that the stability of CAR9 is affected by LOT1 less CAR9 proteins were accumulated and more were ubiquitinated in *** the lot1 car9 and/of f car9 mutants were hyposerisitive to ABA,the hyposensitive phenotype of loticould be rescued by CAR9 ***,our study reveals that LOT1 regulates plant tolerance to drought stress by affecting ABA signaling through regulating the stability and dynamic localization of CAR9.

关键词： LOT1 CAR9 Drought tolerance ABA signaling Stomata transpiration

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Identification of Fusarium wilt resistance gene SiRLK1 in Sesamum indicum L.

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The Crop Journal 2024年第1期

作者： Yinghui Duan Wenwen Qu Shuxian Chang Ming Ju Cuiying Wang Cong Mu Hengchun Cao Guiting Li qiuzhen tian Qin Ma Zhanyou Zhang Haiyang Zhang Hongmei Miao

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An MCIA-like complex is required for mitochondrial complex Ⅰ assembly and seed development in maize

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Molecular Plant 2022年第9期15卷 1470-1487页

作者： Gang Wang Yongyan Wang Jiacheng Ni Rongrong Li Fengling Zhu Ruyin Wang qiuzhen tian Qingwen Shen Qinghua Yang Jihua Tang Monika W.Murcha Guifeng Wang National Key Laboratory of Wheat and Maize Crops Science CIMMYT--China Joint Center of Wheat and MaizeCenter for Crop Genome EngineeringCollege of AgronomyHenan Agricultural UniversityZhengzhou 450046China Shanghai Key Laboratory of Bio-Energy Crops School of Life SciencesShanghai UniversityShanghai 200444China School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghai 200240China School of Molecular Sciences&The ARC Centre of Excellence in Plant Energy Biology The University of Western Australia35 Stirling HighwayCrawleyPerthWA 6009Australia The Shennong Laboratory ZhengzhouHenan 450002China

During adaptive radiation,mitochondria have co-evolved with their hosts,leading to gain or loss of subunits and assembly factors of respiratory *** mitochondrial complex Ⅰ harbors40 nuclearand 9 mitochondrial-encoded subunits,and is formed by stepwise assembly during which different intermediates are integrated via various assembly *** mammals,the mitochondrial complex Ⅰ intermediate assembly(MCIA)complex is required for building the membrane arm ***,plants have lost almost all of the MCIA complex components,giving rise to the hypothesis that plants follow an ancestral pathway to assemble the membrane arm ***,we characterize a maize crumpled seed mutant,crk1,and reveal by map-based cloning that CRK1 encodes an ortholog of human complex Ⅰ assembly factor 1,zNDUFAF1,the only evolutionarily conserved MCIA subunit in ***1 is localized in the mitochondria and accumulates in two intermediate complexes that contain complex Ⅰ membrane arm *** of zNDUFAF1 results in severe defects in complex Ⅰ assembly and activity,a cellular bioenergetic shift to aerobic glycolysis,and mitochondrial ***,we found that zNDUFAF1,the putative mitochondrial import inner membrane translocase ZmTIM17-1,and the isovaleryl-coenzyme A dehydrogenase ZmIVD1 interact each other,and could be co-precipitated from the mitochondria and co-migrate in the same assembly *** of either ZmTIM17-1 or ZmIVD1 could lead to the significantly reduced complex Ⅰ stability and activity as well as defective *** results suggest that zNDUFAF1,ZmTIM17-1 and ZmIVD1 probably form an MCIA-like complex that is essential for the biogenesis of mitochondrial complex Ⅰ and seed development in *** findings also imply that plants and mammals recruit MCIA subunits independently for mitochondrial complex Ⅰ assembly,highlighting the importance of parallel evolution in mitochondria adaptation to their hosts.

关键词： assembly factor CIA30 evolution maize MCIA complex mitochondria seed development

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牡丹切花能荷水平及能量代谢与瓶插品质的关系

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西北植物学报 2020年第9期40卷 1558-1565页

作者：史田孔秋真秦绍玲周爽史国安洛阳国家牡丹园河南洛阳471003 河南科技大学牡丹学院洛阳市牡丹生物学重点实验室河南洛阳471023 洛阳市绿化管理中心河南洛阳471003

为了揭示牡丹切花能量调控规律及其与切花瓶插品质的关系,以发育至二级状态的‘洛阳红’切花为试材,研究高能荷水平(大田自然生长,FNG)和低能荷水平(自然生长采后5~6℃贮藏15 d,PCS)状态下切花瓶插品质与能荷以及能量代谢的敏感基因SnRK... 详细信息

为了揭示牡丹切花能量调控规律及其与切花瓶插品质的关系,以发育至二级状态的‘洛阳红’切花为试材,研究高能荷水平(大田自然生长,FNG)和低能荷水平(自然生长采后5~6℃贮藏15 d,PCS)状态下切花瓶插品质与能荷以及能量代谢的敏感基因SnRK1表达的关系。结果表明:(1)与FNG(高能荷)相比,PCS(低能荷)明显加快瓶插过程中牡丹切花开放和衰老进程,显著缩短瓶插寿命和减小最大花径,并提早呼吸跃变峰值,提高呼吸速率,加快蔗糖降解,导致花瓣琥珀酸脱氢酶(SDH)和线粒体复合体Ⅳ(CCO)活性下降加快,造成ATP和EC水平快速下降,引起PsSnRK1的表达水平上调时间提前,表达水平提高。(2)PsSnRK1基因能够对牡丹花瓣能量匮乏快速响应,能荷水平下降可诱导PsSnRK1基因表达上调。研究发现,冷藏后花瓣能荷水平下降是导致牡丹切花瓶插过程中花朵快速衰老的重要因素之一,花瓣能量状况在调控牡丹切花瓶插品质过程中起着重要作用,并为通过调节牡丹切花能量水平提高切花瓶插品质提供一种新策略。

关键词：牡丹切花能荷状态瓶插品质

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Milankovitch theory and monsoon

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The Innovation 2022年第6期3卷 77-91页

作者： Hai Cheng Hanying Li Lijuan Sha Ashish Sinha Zhengguo Shi qiuzhen Yin Zhengyao Lu Debo Zhao Yanjun Cai Yongyun Hu Qingzhen Hao Jun tian Gayatri Kathayat Xiyu Dong Jingyao Zhao Haiwei Zhang Institute of Global Environmental Change Xi’an Jiaotong UniversityXi’an 710049China State Key Laboratory of Loess and Quaternary Geology Institute of Earth EnvironmentChinese Academy of SciencesXi’an 710061China Department of Earth Sciences California State UniversityDominguez HillsCarsonCA 90747USA Georges Lemaître Center for Earth and Climate Research Earth and Life InstituteUniversitéCatholique de Louvain1348 Louvain-la-NeuveBelgium Department of Physical Geography and Ecosystem Science Lund UniversitySölvegatan 1222362 LundSweden Key Laboratory of Marine Geology and Environment Institute of OceanologyChinese Academy of SciencesQingdao 266071China Department of Atmospheric and Oceanic Sciences School of PhysicsPeking UniversityBeijing 100871China Key Laboratory of Cenozoic Geology and Environment Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China State Key Laboratory of Marine Geology Tongji UniversityShanghai 200092China

The widely accepted“Milankovitch theory”explains insolation-induced waxing and waning of the ice sheets and their effect on the global climate on orbital *** the past half century,however,the theory has often come under scrutiny,especially regarding its“100-ka problem.”Another drawback,but the one that has received less attention,is the“monsoon problem,”which pertains to the exclusion of monsoon dynamics in classic Milankovitch theory even though the monsoon prevails over the vast low-latitude(30N to30S)region that covers half of the Earth’s surface and receives the bulk of solar *** this review,we discuss the major issues with the current form of Milankovitch theory and the progress made at the research forefront.

关键词： monsoon latitude Earth

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机器人辅助关节镜下重建前交叉韧带的疗效

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中华创伤杂志 2022年第2期38卷 142-148页

作者：张丽梁求真赵赞栋康鑫田斌任博张宪董补怀郑江张亮西安交通大学附属红会医院运动医学中心西安710054 西安交通大学附属红会医院麻醉科西安710054

目的比较机器人辅助关节镜下重建与单纯关节镜下重建前交叉韧带(ACL)的临床疗效。方法采用回顾性队列研究分析2020年1月至2020年9月西安交通大学附属红会医院收治的37例ACL撕裂患者的临床资料,其中男24例,女13例;年龄16~45岁[(30.7±9.8... 详细信息

目的比较机器人辅助关节镜下重建与单纯关节镜下重建前交叉韧带(ACL)的临床疗效。方法采用回顾性队列研究分析2020年1月至2020年9月西安交通大学附属红会医院收治的37例ACL撕裂患者的临床资料,其中男24例,女13例;年龄16~45岁[(30.7±9.8)岁]。17例在机器人辅助关节镜下行ACL重建(机器人组),20例在单纯关节镜下行ACL重建(单纯关节镜组)。比较两组手术时间、导针钻孔次数、骨道位点的准确性(骨道中心点距理想解剖点的距离)、围术期并发症情况。术前、术后4个月及末次随访时采用Lachman试验、KT-2000测量数值评估膝关节稳定情况,采用Lysholm评分、国际膝关节文献委员会(IKDC)评分及关节屈伸活动度评估膝关节功能恢复情况。结果患者均获随访12~18个月[(13.1±4.1)个月]。机器人组手术时间为(83.8±11.3)min,明显长于单纯关节镜组的(50.4±9.1)min(P0.05)。两组均未见围术期并发症。术前、术后4个月及末次随访时两组间Lachman试验、KT-2000测量数值、Lysholm评分、IKDC评分及关节屈伸活动度差异无统计学意义(P均>0.05);两组内术后4个月及末次随访时以上相关指标较术前显著改善(P均0.05)。结论机器人辅助关节镜下重建ACL较单纯关节镜下重建ACL手术时间稍长,但可一次性制备具有良好位点及方向的骨道,获得与单纯关节镜下重建ACL相似的关节稳定性及功能恢复。

关键词：前交叉韧带机器人外科手术,计算机辅助软组织损伤

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体外培养山羊成纤维细胞系方法的建立

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中国农业大学学报 2006年第1期11卷 29-34页

作者：潘求真田亮徐曙光韩红兵李佳李海孙书锋连正兴杨宁李宁谭景和中国农业大学动物科学技术学院北京100094 东北农业大学生命科学学院哈尔滨150030

用山羊组织块和0．25％的胰蛋白酶消化培养法获得正常传代细胞，对于出现的上皮样细胞和成纤维样细胞混合生长的问题，则根据两者贴壁紧实程度不同，用0．05％的胰酶-EDTA进行不同时间的消化，将其分离纯化。纯化的成纤维体细胞经数次... 详细信息

用山羊组织块和0．25％的胰蛋白酶消化培养法获得正常传代细胞，对于出现的上皮样细胞和成纤维样细胞混合生长的问题，则根据两者贴壁紧实程度不同，用0．05％的胰酶-EDTA进行不同时间的消化，将其分离纯化。纯化的成纤维体细胞经数次传代培养后，进行冷冻一解冻检验表明仍具有正常的传代能力。各代体细胞的核型分析表明，在体外培养至20～30代成纤维细胞的细胞形态（为梭形细胞，高度汇合后呈火焰状）、细胞周期以及核型均为正常，符合体细胞克隆转基因的基本要求。

关键词：山羊成纤维细胞体外培养

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