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基于代谢组学研究高脂诱导MAFLD模型大鼠血清脂质特征

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医学研究生学报 2022年第5期35卷 455-459页

作者：宋素菲赵冬梅白凤媛孙涛郑继贤徐秋玲刘涛海南医学院第一附属医院中医科海口570102 海南医学院第一附属医院针灸推拿科海口570102 海南医学院中医学院海口571199

目的代谢相关性脂肪肝(MAFLD)以肝细胞脂肪沉积和血脂异常为特征。文章旨在基于代谢组学探讨高脂诱导的MAFLD模型大鼠血清脂质代谢物特点。方法12只SD雄性大鼠随机数字表法分为正常组和模型组,每组6只,分别予普通饲料和高脂饲料喂养4周... 详细信息

目的代谢相关性脂肪肝(MAFLD)以肝细胞脂肪沉积和血脂异常为特征。文章旨在基于代谢组学探讨高脂诱导的MAFLD模型大鼠血清脂质代谢物特点。方法12只SD雄性大鼠随机数字表法分为正常组和模型组,每组6只,分别予普通饲料和高脂饲料喂养4周。油红O染色观察两组大鼠肝脏脂质沉积情况,利用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UHPLC-QE-MS)非靶标代谢组学技术,筛选出潜在的差异代谢物并进行脂质富集分析。结果油红O染色结果显示模型组肝细胞内出现大量大小不等的红色脂滴堆积。代谢组学筛选出85个潜在差异代谢物,43个脂质差异代谢物,7种脂类物质,其中脂肪酸类、固醇脂类和甘油磷脂类差异有统计学意义(P<0.05)。结论MAFLD模型大鼠血清脂质代谢物存在显著变化。

关键词：代谢组学 UHPLC-QE-MS 代谢相关脂肪性肝病

Analysis of In-hospital Neonatal Death in the Tertiary Neonatal Intensive Care Unit in china： A Multicenter Retrospective Study

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Chinese Medical Journal 2016年第22期129卷 2652-2658页

作者： Chen-Hong Wang Li-Zhong Du Xiao-Lu Ma Li-Ping Shi Xiao-Mei Tong Hong Liu Guo-Fang Ding Bin Yi Xin-Nian Pan Dan-Ni Zhong ling Liu Mei Li Cui-Qing Li Shi-Wen Xia Hong-Yun Wang ling He Kun Liang Xiao-Yu Zhou Shu-Ping Han Qin Lyu Yin-Ping qiu Ruo-Bing Shan De-Zhi Mu Xiao-Hong Liu Si-Qi Zhuang Jing Guo Li Liu Jia-Jun Zhu Hong Xiong department of Neonatal Intensive Care Unit Children's Hospital Zhejiang University School of Medicine Hangzhou Zhejiang 310051 China department of Pediatrics Peking University Third Hospital Beijing 100191 China department of Neonatology Beijing Children's Hospital Capital Medical University Beijing 100045 China department of Pediatrics Peking Union Medical College Hospital Chinese Academy of Medical Sciences Beijing 100730 China department of Neonatology Gansu Provincial Maternity and Children-care Hospital Lanzhou Gansu 730050 China department of Neonatology Guangxi Maternal and Child Health Hospital Nanning Guangxi 530003 China department of Neonatology The First Affiliated Hospital of Guangxi Medical University Nanning Guangxi 530021 China department of Neonatology Guiyang Maternal and Child Health Care Hospital Guiyang Guizhou 550003 China department of Neonatology Harbin Children's Hospital Harbin Heilongjiang 150010 China department of Neonatology Children's Hospital of Hebei Province Shijiazhuang Hebei 050031 China department of Neonatology Hubei Maternity and Child Heath Hospital Wuhan Hubei 430070 China department of Neonatal Intensive Care Unit Inner Mongolia Maternal and Child Health Hospital Huhhot Inner Mongolia 010030 China department of Neonatology Jiangxi Provincial Children's Hospital Nanchang Jiangxi 330006 China department of Pediatric First Affiliated Hospital of Kunming Medical University Kunming Yunnan 650032 China department of Neonatology Nanjing Children's Hospital of Nanjing Medical University Nanjing Jiangsu 320100 China department of Neonatology Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University Nanjing Jiangsu 210004 China DeDartment of Neonatal Intensive Care Unit Ningbo Women and Children's Hospital Ningbo Zhejiang 315012 China department of Neonatology General Hospital of Ningxia Medical University Yinchuan Ningxia 750004 China department of Neonatology Qingdao Women and

Background：Globally,the proportion of child deaths that occur in the neonatal period remains a high level of 37-41%.Differences of cause in neonate death exist in different regions as well as in different economic development *** specific aim of this study was to investigate the causes,characteristics,and differences of death in neonates during hospitalization in the tertiary Neonatal Intensive Care Unit （NICU） of ***：All the dead neonates admitted to 26 NICUs were included between January 1,2.1.,and December 31,*** the data were collected retrospectively from clinical records by a designed *** collected from each NICU were delivered to the leading institution where the results were ***：A total of 744 newborns died during the l-year survey,accounting for 1.2% of all the neonates admitted to 26 NICUs and 37.6% of all the deaths in children under 5 years of age in these *** neonate death accounted for 59.3% of all the *** leading causes of death in preterm and term infants were pulmonary disease and infection,*** early neonate period,pulmonary diseases （56.5%） occupied the largest proportion ofpreterm deaths while infection （27%） and neurologic diseases （22%） were the two main causes of term *** late neonate period,infection was the leading cause of both preterm and term neonate *** two-thirds of neonate death occurred after medical care *** the cases who might survive if receiving continuing treatment,parents＇ concern about the long-term outcomes was the main reason of medical care ***：Neonate death still accounts for a high proportion of all the deaths in children under 5 years of *** study showed the majority of neonate death occurred in preterm *** of death varied with the age of death and gestational *** and prompt evaluation of the long-term outcomes should be carried out to guide the critical decision.

关键词： Cause-of-death Mortality Newbom Retrospective Survey

髓鞘少突胶质细胞糖蛋白抗体相关视神经炎不同治疗及预防方案效果分析

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中华医学杂志 2020年第7期100卷 498-503页

作者：谭笑王玲张榆欣刘开群周琳陈冬周满丽邱伟杨晖中山大学中山眼科中心广州510060 江西省南城县人民医院抚州344700 中山大学第三附属医院神经内科广州510060

目的回顾分析不同治疗及预防方案对髓鞘少突胶质细胞糖蛋白抗体相关视神经炎(MOG-ON)患者急性期治疗效果及复发率的影响。方法收集2.1.年1月至2.1.年12月,在中山大学中山眼科中心确诊MOG-ON患者37例。比较激素冲击治疗及大剂量激素(未冲... 详细信息

目的回顾分析不同治疗及预防方案对髓鞘少突胶质细胞糖蛋白抗体相关视神经炎(MOG-ON)患者急性期治疗效果及复发率的影响。方法收集2.1.年1月至2.1.年12月,在中山大学中山眼科中心确诊MOG-ON患者37例。比较激素冲击治疗及大剂量激素(未冲击)治疗对首次发作的MOG-ON患者(初发组)视功能恢复及视网膜神经纤维层厚度影响;比较小剂量激素及小剂量激素联合吗替麦考酚酯(MMF)预防方案,对MOG-ON复发率的影响。结果37例MOG-ON患者中,初发组25例,复发组12例。初发组,采用激素冲击治疗19例,大剂量激素(未冲击)组6例。激素冲击组治疗后1个月视野MD值[(-7±8)dB]较发病时[(-26±11)dB]有显著恢复(P1),而大剂量激素(未冲击)组治疗后6个月视野MD值[(-9±9)dB]才较发病时[(-22.1.)dB]有显著恢复(P1)。两组最佳矫正视力、视野缺损、视诱发电位振幅、视网膜神经纤维层厚度在发病后1、3、6个月差异均无统计学意义(P>0.05)。在复发预防中,复发组小剂量激素联合MMF预防9例(75%),预防前年复发率为0.77(0.21~4.5)次/年,预防后仅一例复发,年复发率降低为0(0~0.41)次/年(P<0.05)。结论MOG-ON急性期激素冲击治疗有可能加快视功能恢复及改善预后,小剂量激素联合MMF方案有可能降低MOG-ON复发率。

关键词：髓鞘少突胶质细胞糖蛋白抗体相关视神经炎治疗预防

前蛋白转化酶枯草溶菌素9抑制剂在急性冠状动脉综合征患者中多维度作用探索的研究进展

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中国心血管病研究 2021年第11期19卷 1050-1056页

作者：袁静徐承义孙玉娟邱令智鄢华武汉科技大学医学院湖北省武汉市430081 武汉科技大学附属武汉亚洲心脏病医院心内科湖北文理学院附属襄阳市中心医院心内科

作为近年发现的一种新型的降脂靶点,PCSK9抑制剂已经被证实可以改善极高危心血管疾病患者的临床预后。本文介绍了PCSK9抑制剂的经典降脂作用及非经典作用途径,其中非经典作用途径包括降低LP(a)、稳定斑块、抑制血小板活化、减少炎症产... 详细信息

作为近年发现的一种新型的降脂靶点,PCSK9抑制剂已经被证实可以改善极高危心血管疾病患者的临床预后。本文介绍了PCSK9抑制剂的经典降脂作用及非经典作用途径,其中非经典作用途径包括降低LP(a)、稳定斑块、抑制血小板活化、减少炎症产生及改善血管内皮功能等。相较于临床常用的传统降脂药物,已有研究证实PCSK9抑制剂联合他汀类药物在显著降低LDL-C的同时,还可进一步降低近期ACS患者不良心血管事件及全因死亡风险。此外,PCSK9抑制剂在ACS患者中由于其非经典途径所带来的潜在额外获益也正逐步被关注。

关键词： PCSK9抑制剂动脉粥样硬化性心脏病经皮冠状动脉介入治疗 PCSK9

2.1.年广东省某三甲医院重点监控品种临床使用情况分析及管理对策思考

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中国合理用药探索 2020年第8期17卷 20-23页

作者：黄玉玲陈秋凤珠海市中西医结合医院药学部珠海519000 中山大学附属第五医院药学部珠海519000

目的:了解2.1.年我院重点监控品种的临床使用特点,为加强重点监控品种管理和促进临床合理用药提供参考依据。方法:制定重点监控目录及管理制度;通过医院信息系统,统计2.1.年重点监控品种的金额和用药频度,利用Excel对数据进行汇总及分析... 详细信息

目的:了解2.1.年我院重点监控品种的临床使用特点,为加强重点监控品种管理和促进临床合理用药提供参考依据。方法:制定重点监控目录及管理制度;通过医院信息系统,统计2.1.年重点监控品种的金额和用药频度,利用Excel对数据进行汇总及分析;利用医院信息软件,随机抽取同期销售金额排名前五的重点监控品种相关病例各50份,分析其临床使用的合理性。结果:2.1.年本院重点监控品种占全院药品销售金额的20.32%;销售金额前10名的重点监控品种占重点监控品种销售金额的39.78%。我院重点监控品种排名前20的药物中,有11个抗菌药物、5个非治疗性辅助用药(包括:免疫调节剂、营养神经类、活菌类、维生素类)、3个中药注射剂、1个PPIs。不合理用药医嘱共81份,占比32.40%。结论:我院重点监控用药品种的监管措施取得了一定成效,在一定程度上促进了临床合理用药,但仍需采取积极有效的监管措施,进一步促进重点监控药品规范合理使用。

关键词：重点监控品种辅助用药合理用药管理对策用药频度

增强自噬水平对脑缺血预处理神经保护机制的影响

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河北医科大学学报 2021年第7期42卷 750-753,764,F0003页

作者：孙原侯晓冬杨延雯李艳玲李弘石秋艳华北理工大学附属医院神经内科河北唐山063000 河北省唐山市人民医院病案科河北唐山063001

目的探讨自噬参与脑缺血预处理神经保护机制的影响。方法使用线栓法制作大鼠脑缺血再灌注模型。100只成年SD大鼠随机分为假手术组(A组)、缺血再灌注组(B组),缺血预处理组(C组),缺血再灌注+自噬抑制剂3-甲基腺嘌呤(3-Methyladenine,3-MA)... 详细信息

目的探讨自噬参与脑缺血预处理神经保护机制的影响。方法使用线栓法制作大鼠脑缺血再灌注模型。100只成年SD大鼠随机分为假手术组(A组)、缺血再灌注组(B组),缺血预处理组(C组),缺血再灌注+自噬抑制剂3-甲基腺嘌呤(3-Methyladenine,3-MA)组(D组),缺血预处理+3-MA组(E组)。D、E组大鼠侧脑室注射3-MA(7.5μg),其余3组大鼠注射相同体积的生理盐水。观察各组脑梗死体积、神经功能缺损评分、自噬系统表达水平及神经元凋亡情况。结果A组脑梗死体积和神经功能障碍评分均为0;C组脑梗死体积和神经功能障碍评分小于B组;D组脑梗死体积和神经功能障碍评分大于B组,小于C组;E组脑梗死体积和神经功能障碍评分大于C组,小于D组,差异有统计学意义(P>0.05)。光学显微镜下观察神经元:A组数量多,形态完整;其余4组数量少,形态破坏,细胞核破裂、固缩,其中最为严重的是B组和C组,D组和E组神经元数量较B组和C组增多,细胞水肿及细胞核损伤减轻。A组Beclin-1、LC3-Ⅱ及TUNEL染色阳性细胞表达少见;4组Beclin-1、LC3-Ⅱ及TUNEL染色阳性细胞表达多。C组Beclin-1、LC3-Ⅱ阳性细胞数大于B组,TUNEL染色阳性细胞数小于B组;D组Beclin-1、LC3-Ⅱ阳性细胞数及TUNEL染色阳性细胞数小于B组和C组,TUNEL染色阳性细胞数大于B组和C组;E组Beclin-1、LC3-Ⅱ阳性细胞数小于C组,TUNEL染色阳性细胞数小于D组,差异有统计学意义(P<0.05)。结论缺血预处理后细胞自噬能力加强,抑制神经元凋亡,使缺血再灌注后的颅内神经元受到保护。

关键词：再灌注损伤缺血预处理自噬

Efficacy and safety of sirolimus early conversion protocol in liver transplant patients with hepatocellular carcinoma:A single-arm, multicenter, prospective study

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Hepatobiliary & Pancreatic Diseases International 2022年第2期21卷 106-112页

作者： Ren-Yi Su sun-Bin ling Qiao-Nan Shan Xu-Yong Wei Rui Wang Chang-Ku Jia Li Zhuang Tian Shen Li-Min Ding Zhi-Dan Xu Lai-Bang Luo Li-Bo sun Guang-Ming Li Tai-Shi Fang Nan Jiang Kun Zhang Zhao-Jie Su Zhi-Hai Peng Ren Lang Tao Jiang Qiang He Lin-Sen Ye Yang Yang Yu-Ting He Wen-Zhi Guo Liu-Gen Lan Xu-Yong sun Dong Chen Zhi-Shui Chen Da-Wei Zhou Shao-Jun Ye Qi-Fa Ye Min Tian Jian-Hua Shi Bo Wang Jiang Liu Qian Lu Wei Rao Jin-Zhen Cai Tao Lv Jia-Yin Yang Pu-Sen Wang Lin Zhong Jing-Sheng Ma Qi-Gen Li Sheng-Dong Wu Chang-Jiang Lu Cai-De Lu Dong-Hua Zhang Xuan Wang Zi-Qiang Li Mu-Jian Teng Jun-Jie Li Wen-Tao Jiang Jian-Hua Li Quan-Bao Zhang Ning-Qi Zhu Zheng-Xin Wang Kang He Qiang Xia Shao-Hua Song Zhi-Ren Fu Wei qiu Guo-Yue Lv Rui-Peng Song Ji-Zhou Wang Zheng Wang Jian Zhou Gang Chen Ying-Peng Zhao Li Li Ze-Min Hu Qi-Jie Luo Zhong-Zhou Si Bin Xie Xiao-Shun He Zhi-Yong Guo Shu-Sen Zheng Xiao Xu department of Hepatobiliary and Pancreatic Surgery The Center for Integrated Oncology and Precision MedicineAffiliated Hangzhou First People’s HospitalZhejiang University School of MedicineHangzhou 310006China NHC Key Laboratory of Combined Multi-organ Transplantation Hangzhou 310003China department of Hepatobiliary and Pancreatic Surgery Shulan(Hangzhou)HospitalZhejiang Shuren University School of MedicineHangzhou 310022China department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China department of Transplantation Jiangxi Provincial People’s Hospital Affiliated to Nanchang UniversityNanchang 330006China Liver Transplantation Center Beijing You’an HospitalCapital Medical UniversityBeijing 100069China department of Hepatic Surgery National Clinical Research Center for Infectious DiseaseShenzhen Third People’s HospitalThe Second Affiliated Hospital of Southern University of Science and TechnologyShenzhen 518112China department of General Surgery Xiang’an Hospital of Xiamen UniversityXiamen 361000China Deartment of Hepatobiliary and Pancreaticosplenic Surgery Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China department of Hepatic Surgery and Liver Transplantation Center The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630China department of Hepatobiliary and Pancreatic Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China department of Liver Transplantation The Second Affiliated Hospital of Guangxi Medical UniversityNanning 530000China Institute of Organ Transplantation Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Institute of Hepatobiliary Diseases of wuhan university Transplant Center of Wuhan UniversityHubei Key Laboratory of Medical Technology on TransplantationZhongnan Hospital of Wuhan UniversityWuhan 430071China department of Hepatobiliary Surgery The First Affiliated Hos

Mammalian target of rapamycin(m TOR) inhibitor as an attractive drug target with promising antitumor effects has been widely investigated. High quality clinical trial has been conducted in liver transplant(LT) recipients in Western countries. However, the pertinent studies in Eastern world are paucity. Therefore, we designed a clinical trial to test whether sirolimus can improve recurrence-free survival(RFS) in hepatocellular carcinoma(HCC) patients beyond the Milan criteria after LT. This is an open-labeled, single-arm, prospective, multicenter, and real-world study aiming to evaluate the clinical outcomes of early switch to sirolimus-based regimens in HCC patients after LT. Patients with a histologically proven HCC and beyond the Milan criteria will be enrolled. The initial immunosuppressant regimens are center-specifc for the frst 4-6 weeks. The following regimens integrated sirolimus into the regimens as a combination therapy with reduced calcineurin inhibitors based on the condition of patients and centers. The study is planned for 4 years in total with a 2-year enrollment period and a 2-year follow-up. We predict that sirolimus conversion regimen will provide survival benefts for patients particular in the key indicator RFS as well as better quality of life. If the trial is conducted successfully, we will have a continued monitoring over a longer follow-up time to estimate indicator of overall survival. We hope that the outcome will provide better evidence for clinical decision-making and revising treatment guidelines based on Chinese population data.

关键词： Sirolimus Immunosuppressive agents Hepatocellular carcinoma Liver transplantation Conversion

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2型糖尿病合并心房颤动的危险因素分析

中国医药导报 2019年第11期16卷 105-108页

作者：邱娜凌宏威周冬梅孙娟应长江李伟徐州医科大学附属医院内分泌科江苏徐州221006

目的研究2型糖尿病(T2DM)发生心房颤动(AF)的危险因素。方法选择2.1.年1月～2.1.年1月于徐州医科大学附属医院就诊的T2DM患者530例(对照组),T2DM合并AF患者533例(T2DM合并AF组),并将533例T2DM合并AF患者分为2个亚组,包括合并阵发性心房... 详细信息

目的研究2型糖尿病(T2DM)发生心房颤动(AF)的危险因素。方法选择2.1.年1月～2.1.年1月于徐州医科大学附属医院就诊的T2DM患者530例(对照组),T2DM合并AF患者533例(T2DM合并AF组),并将533例T2DM合并AF患者分为2个亚组,包括合并阵发性心房颤动患者220例(T2DM合并PAF组)与合并非阵发性心房颤动患者313例(T2DM合并NPAF组)。收集其相关指标进行单因素方差分析、Logistic回归分析,筛选出影响因素,并用受试者工作特征曲线(ROC)分析其预测价值。结果单因素分析显示,对照组与T2DM合并AF组的尿酸、血糖、糖化血红蛋白、胆红素、低密度脂蛋白及胆固醇水平差异有统计学意义(P 1.005,P 1.398,P 10,P <0.05)是T2DM患者发生AF的保护因素;ROC曲线分析提示上述指标对房颤发生均有一定的预测价值。结论在T2DM患者中,尿酸及糖化血红蛋白是房颤发生的独立危险因素;而总胆红素、低密度脂蛋白及胆固醇是发生房颤的保护性因素。

关键词：糖尿病房颤尿酸糖化血红蛋白血脂

3D打印手术导板辅助显微根尖手术的临床应用

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中山大学学报（医学版） 2018年第4期39卷 636-641页

作者：邱小玲林桂珍贾搏李欣邢云娣陈蕾南方医科大学口腔医院//广东省口腔医院综合急诊科广东广州510280 南方医科大学口腔医院//广东省口腔医院口腔颌面外科广东广州510280

【目的】评价数字化术前诊断设计、3D打印技术及手术导板在显微根尖手术中应用效果,有效控制根尖手术中风险,实现微创及精准治疗的目的。【方法】选择10例需要行显微根尖外科手术的患牙,使用3D导板辅助下手术,评估术中去骨的微创度和术... 详细信息

【目的】评价数字化术前诊断设计、3D打印技术及手术导板在显微根尖手术中应用效果,有效控制根尖手术中风险,实现微创及精准治疗的目的。【方法】选择10例需要行显微根尖外科手术的患牙,使用3D导板辅助下手术,评估术中去骨的微创度和术后患者疼痛度。【结果】3D手术导板辅助显微根尖手术术中去骨量最大直径介于3~4 mm之间,平均值3.27,标准差0.35 mm;骨开窗的中心与牙根长轴的中心误差均小于0.50 mm,平均值0.24 mm,标准差0.15 mm;术后患者的出血、肿胀反应明显减轻,根据VAS疼痛量表一般表现为轻度到中度。【结论】3D手术导板辅助显微根尖手术可以做到治疗微创、精准、快速,降低术中、术后出血、肿胀等并发症。

关键词： 3D打印手术导板显微根尖手术慢性根尖周炎