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Energy & Environmental Materials 2024年第4期7卷 93-98页

作者： Junwu Sang Bin Tang Yong qiu Yongzheng fang Kecheng Pan Zhen zhou Interdisciplinary Research Center for Sustainable Energy Science and Engineering (IRC4SE2) School of Chemical Engineering Zhengzhou University

All-solid-state lithium metal batteries (ASSLMBs) with solid electrolytes (SEs)have emerged as a promising alternative to liquid electrolyte-based Li-ion batteries due to their higher energy density and ***,since ASSLMBs lack the wetting properties of liquid electrolytes,they require stacking pressure to prevent contact loss between electrodes and SEs.

关键词： critical current density solid electrolyte solid-state lithium metal batteries stacking pressure

Interface engineering enabled sintering-resistant metal nanocatalysts via reverse Ostwald ripening

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Science China Chemistry 2024年第9期67卷 2789-2791页

作者： Ying Sun Wenhui fang Jieshan qiu Institute of Clean Energy Chemistry Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials of Liaoning ProvinceCollege of ChemistryLiaoning UniversityShenyang 110036China State Key Laboratory of Chemical Resource Engineering College of Chemical EngineeringBeijing University of Chemical TechnologyBeijing 100029China

The design and fabrication of advanced nanocatalysts with the high activity,selectivity,and superior durability remains a big challenge in industrial catalysis[1,2].The sintering of nanocatalysts under the thermally and/or chemically triggered conditions is a bottle-neck issue,which seriously decreases the amount of the exposed active sites and changes the original electronic structure of catalysts,resulting in the irreversible catalyst deactivation and high capital and time cost in industrial operation[3].

关键词： durability irreversible catalysis

VSITA, an Improved Approach of Target Amplification in the Identification of Viral Pathogens

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Biomedical and Environmental Sciences 2018年第4期31卷 272-279页

作者： ZHANG Yi ZHANG Chen LI Bo LI Yang HE Xiao zhou LI Acher WU Wei DUAN Su Xia qiu fang zhou WANG Ji SHEN Xin Xin YANG Meng Jie LI De Xin MA Xue Jun Key Laboratory for Medical Virology National Health and Family Planning Commission National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences Pediatric Research Institute Children's Hospital of Hebei Province

Objective Unbiased next generation sequencing（NGS） is susceptible to interference from host or environmental sequences. Consequently, background depletion and virome enrichment techniques are usually needed for clinical samples where viral load is much lower than background sequences. Methods A viral Sequence Independent Targeted Amplification（VSITA） approach using a set of non-ribosomal and virus-enriched octamers（V8） was developed and compared with traditionally used random hexamers（N6）. Forty-five archived clinical samples of different types were used in parallel to compare the V8 and N6 enrichment performance of viral sequences and removal performance of ribosomal sequences in the step of reverse transcription followed by quantitative PCR（qP CR）. Ten sera samples from patients with fever of unknown origin and 10 feces samples from patients with diarrhea of unknown origin were used in comparison of V8 and N6 enrichment performance following NGS analysis. Results A minimum 30 hexamers matching to viral reference sequences（sense and antisense） were selected from a dataset of random 4,096（4~6） hexamers（N6）. Two random nucleotides were added to the 5＇ end of the selected hexamers, and 480（30 × 4~2） octamers（V8） were obtained. In general, VSITA approach showed higher enrichment of virus-targeted c DNA and enhanced ability to remove unwanted ribosomal sequences in the majorities of 45 predefined clinical samples. Moreover, VSITA combined with NGS enabled to detect not only more viruses but also achieve more viral reads hit and higher viral genome coverage in 20 clinical samples with diarrhea or fever of unknown origin. Conclusion The VSITA approach designed in this study is demonstrated to possess higher sensitivity and broader genome coverage than traditionally used random hexamers in the NGS-based identification of viral pathogens directly from clinical samples.

关键词： Virus Next generation sequencing Non-ribosomal Virus targeted

Abiotic stress treatment reveals expansin like A gene OfEXLA1 improving salt and drought tolerance of Osmanthus fragrans by responding to abscisic acid

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Horticultural Plant Journal 2024年第2期10卷 573-585页

作者： Bin Dong Qianqian Wang Dan zhou Yiguang Wang Yunfeng Miao Shiwei Zhong qiu fang Liyuan Yang Zhen Xiao Hongbo Zhao School of Landscape Architecture Zhejiang Agriculture and Forestry UniversityHangzhouZhejiang 311300China Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants HangzhouZhejiang 311300China Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants HangzhouZhejiang 311300China

Sweet osmanthus(Osmanthus fragrans) is a having general approval aromatic tree in China that is widely applied to landscaping and gardening. However, the evergreen tree adaptability is limited by many environmental stresses. Currently, limited information is available regarding the genetic analysis and functional identification of expansin genes in response to abiotic stress in sweet osmanthus. In this study, a total of 29 expansin genes were identified and divided into four groups by genome-wide analysis from the sweet osmanthus genome. Transcriptome and quantitative Real-time PCR analysis showed that the cell wall-localized protein expansin-like A(OfEXLA1) gene was significantly induced by salt and drought treatment. Histochemical GUS staining of transgenic Arabidopsis lines in which GUS activity was driven with the OfEXLA1 promoter, GUS activity was significantly induced by salt, drought, and exogenous abscisic acid(ABA). In yeast, we found OfEXLA1overexpression significantly improved the population of cells compared with wild-type strains after NaCl and polyethylene glycol(PEG)treatment. Additionally, OfEXLA1 overexpression not only promoted plant growth, but also improved the salt and drought tolerance in Arabidopsis. To gain insight into the role of ABA signaling in the regulation of OfEXLA1 improving abiotic tolerance in sweet osmanthus, four differentially expressed ABA Insensitive 5(ABI5)-like genes(OfABL4, OfABL5, OfABL7, and OfABL8) were identified from transcriptome, and dualluciferase(dual-LUC) and yeast one hybrid(Y1H) assay showed that OfABL4 and OfABL5 might bind to OfEXLA1 promoter to accumulate the OfEXLA1 expression by responding to ABA signaling to improve abiotic tolerance in sweet osmanthus. These results provide the information for understanding the molecular functions of expansin-like A gene and molecular breeding of sweet osmanthus in future.

关键词： Osmanthus fragrans Abiotic tolerance Expansin Abscisic acid

Construction of CDKN2A-related competitive endogenous RNA network and identification of GAS5 as a prognostic indicator for hepatocellular carcinoma

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World Journal of Gastrointestinal Oncology 2024年第4期16卷 1514-1531页

作者： Yong Pan Yi-Ru Zhang Ling-Yun Wang Li-Na Wu Ying-qiu Ma zhou fang Shi-Bo Li Department of Infectious Disease Zhoushan HospitalWenzhou Medical UniversityZhoushan 316021Zhejiang ProvinceChina State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases The First Affiliated Hospital of Zhejiang UniversityHangzhou 310003Zhejiang ProvinceChina Department of Infectious Disease The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325035Zhejiang ProvinceChina

BACKGROUND Competitive endogenous RNA(ceRNA)is an innovative way of gene expression modulation,which plays a crucial part in ***,the intricacy and behavioral characteristics of the ceRNA network in hepatocellular carcinoma(HCC)remain *** To establish a cyclin dependent kinase inhibitor 2A(CDKN2A)-related ceRNA network and recognize potential prognostic indicators for *** The mutation landscape of CDKN2A in HCC was first explored using the cBioPortal *** expression analysis was implemented between CDKN2Ahigh and CDKN2Alow expression HCC *** targeted microRNAs were predicted by lncBasev3.0,and the targeted mRNAs were predicted by miRDB,and Targetscan *** univariate and multivariate analysis were utilized to identify independent prognostic *** CDKN2A was frequently mutated and deleted in *** single-cell RNA-sequencing analysis revealed that CDKN2A participated in cell cycle *** CDKN2A-related ceRNA network-growth arrest specific 5(GAS5)/miR-25-3p/SRY-box transcription factor 11(SOX11)was successfully ***5 was recognized as an independent prognostic biomarker,whose overexpression was correlated with a poor prognosis in HCC *** association between GAS5 expression and methylation,immune infilt-ration was ***,traditional Chinese medicine effective components targeting GAS5 were *** This CDKN2A-related ceRNA network provides innovative insights into the molecular mechanism of HCC formation and ***,GAS5 might be a significant prognostic biomarker and therapeutic target in HCC.

关键词： Hepatocellular carcinoma Competitive endogenous RNA Molecular mechanism Prognosis Biomarker

Is Peer Victimization Associated with Higher Online Trolling among Adolescents?The Mediation of Hostile Attribution Bias and the Moderation of Trait Mindfulness

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International Journal of Mental Health Promotion 2024年第8期26卷 623-632页

作者： Yuedong qiu Qi Sun Jie zhou Ni Jiang Wenyu Zeng Biyun Wu fang Li School of Psychology Sichuan Normal UniversityChengdu610066China Center of Adolescent Growth Services Sichuan Dazhu Middle SchoolDazhou635100China Center of Mental Health Education Guangzhou University of Chinese MedicineGuangzhou510470China Sichuan Key Laboratory of Psychology and Behavior of Discipline Inspection and Supervision Sichuan Normal UniversityChengdu610066China

Background:In recent years,online trolling has garnered significant attention due to its detrimental effects on mental health and social *** current study examined the influence of peer victimization on adolescent online trolling behavior,proposing that hostile attribution bias mediated this relationship and that trait mindfulness moderated both the direct and indirect ***:A total of 833 Chinese adolescents completed the measurements of peer victimization,hostile attribution bias,trait mindfulness,and online *** mediation analysis was performed to examine the relationships between these ***:After controlling for gender and residential address,the study found a significant positive correlation between peer victimization and online trolling,with hostile attribution bias serving as a *** addition,trait mindfulness moderated the direct relationship between peer victimization and online ***,the effect of peer victimization on online trolling was attenuated when adolescents had high levels of trait *** results of the study emphasized the joint role of peer and personal factors in adolescents’online trolling behavior and provide certain strategies for intervening in adolescents’online trolling ***:The results of the study suggest that strategies focusing on peer support and mindfulness training can have a positive impact on reducing online trolling behavior,promoting adolescents’mental health,and their long-term development.

关键词： Online trolling peer victimization hostile attribution bias trait mindfulness adolescent

Thermally Conductive,Healable Glass Fiber Cloth Reinforced Polymer Composite based onβ-Hydroxyester Bonds Crosslinked Epoxy with Improved Heat Resistance

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Chinese Journal of Polymer Science 2024年第5期42卷 643-654,I0009页

作者： fang Chen Xiao-Yan Pang Ze-Ping Zhang Min-Zhi Rong Ming-qiu Zhang Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education GD HPPC LabIGCMESchool of ChemistrySun Yat-sen UniversityGuangzhou510275China Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center Jieyang515200China

To simultaneously endow thermal conductivity,high glass transition temperature(Tg)and healing capability to glass fiber/epoxy(GFREP)composite,dynamic crosslinked epoxy resin bearing reversibleβ-hydroxyl ester bonds was reinforced with boron nitride nanosheets modified glass fiber cloth(GFC@BNNSs).The in-plane heat conduction paths were constructed by electrostatic self-assembly of polyacrylic acid treated GFC and polyethyleneimine decorated ***,the GFC@BNNSs were impregnated with the mixture of lower concentration(3-glycidyloxypropyl)trimethoxysilane grafted BN micron sheets,3,4-epoxycyclohexylmethyl 3,4-epoxycyclohexanecarboxylate and hexahydro-4-methylphthalic anhydride,which accounted for establishing the through-plane heat transport pathways and avoiding serious deterioration of mechanical *** resultant GFREP composite containing less boron nitride particles(17.6 wt%)exhibited superior in-plane(3.29 W·m^(-1)·K^(-1))and through-plane(1.16 W·m^(-1)·K^(-1))thermal conductivities,as well as high Tg of 204℃(Tg of the unfilled epoxy=177℃).The reversible transesterification reaction enabled closure of interlaminar cracks within the composite,achieving decent healing efficiencies estimated by means of tensile strength(71.2%),electrical breakdown strength(83.6%)and thermal conductivity(69.1%).The present work overcame the disadvantages of conventional thermally conductive composites,and provided an efficient approach to prolong the life span of thermally conductive GFREP laminate for high-temperature resistant integrated circuit application.

关键词： Thermally conductive composites Boron nitride High-temperature resistance β-Hydroxyl ester bond Healing

OsNPF3.1,a nitrate,abscisic acid and gibberellin transporter gene,is essential for rice tillering and nitrogen utilization efficiency

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Journal of Integrative Agriculture 2024年第4期23卷 1087-1104页

作者： Junnan Hang Bowen Wu Diyang qiu Guo Yang Zhongming fang Mingyong Zhang Institute of Rice Industry Technology Research/Key Laboratory of Functional Agriculture Guizhou Provincial Department of Education/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous RegionMinistry of Education/Key Laboratory of Molecular Breeding for Grain and Oil Crops in Guizhou Province/College of Agricultural SciencesGuizhou UniversityGuiyang 550025China Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement/Guangdong Provincial Key Laboratory of Applied Botany/South China Botanical Garden Chinese Academy of SciencesGuangzhou 510650China Institute of Fruit Tree Research Guangdong Academy of Agricultural SciencesGuangzhou 510640China

Low-affinity nitrate transporter genes have been identified in subfamilies 4-8 of the rice nitrate transporter 1(NRT1)/peptide transporter family(NPF),but the OsNPF3 subfamily responsible for nitrate and phytohormone transport and rice growth and development remains *** this study,we described OsNPF3.1 as an essential nitrate and phytohormone transporter gene for rice tillering and nitrogen utilization efficiency(NUtE).OsNPF3.1 possesses four major haplotypes of its promoter sequence in 517 cultivars,and its expression is positively associated with tiller *** expression was higher in the basal part,culm,and leaf blade than in other parts of the plant,and was strongly induced by nitrate,abscisic acid(ABA)and gibberellin 3(GA_3)in the root and shoot of *** experiments demonstrated that OsNPF3.1 is a pH-dependent low-affinity nitrate transporter,with rice protoplast uptake assays showing it to be an ABA and GA_3 ***3.1 overexpression significantly promoted ABA accumulation in the roots and GA accumulation in the basal part of the plant which inhibited axillary bud outgrowth and rice tillering,especially at high nitrate *** NUtE of OsNPF3.1-overexpressing plants was enhanced under low and medium nitrate concentrations,whereas the NUtE of OsNPF3.1 clustered regularly interspaced short palindromic repeats(CRISPR)plants was increased under high nitrate *** results indicate that OsNPF3.1 transports nitrate and phytohormones in different rice tissues under different nitrate *** altered OsNPF3.1 expression improves NUtE in the OsNPF3.1-overexpressing and CRISPR lines at low and high nitrate concentrations,respectively.

关键词： rice tillering grain yield phytohormone nitrate transporter nitrogen utilization efficiency

Privacy-Preserving Healthcare and Medical Data Collaboration Service System Based on Blockchain and Federated Learning

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Computers, Materials & Continua 2024年第8期80卷 2897-2915页

作者： fang Hu Siyi qiu Xiaolian Yang ChaoleiWu Miguel Baptista Nunes Hui Chen College of Information Engineering Hubei University of Chinese MedicineWuhan430065China College of Information Engineering Zhongnan University of Economics and LawWuhan430073China School of Advanced Technologies Xi’an Jiaotong-Liverpool UniversitySuzhou215400China School of Information Management Central China Normal UniversityWuhan430079China

As the volume of healthcare and medical data increases from diverse sources,real-world scenarios involving data sharing and collaboration have certain challenges,including the risk of privacy leakage,difficulty in data fusion,low reliability of data storage,low effectiveness of data sharing,*** guarantee the service quality of data collaboration,this paper presents a privacy-preserving Healthcare and Medical Data Collaboration Service System combining Blockchain with Federated Learning,termed *** system is composed of three layers:Data extraction and storage,data management,and data *** on healthcare and medical data,a healthcare and medical blockchain is constructed to realize data storage,transfer,processing,and access with security,real-time,reliability,and *** improved master node selection consensus mechanism is presented to detect and prevent dishonest behavior,ensuring the overall reliability and trustworthiness of the collaborative model training ***,healthcare and medical data collaboration services in real-world scenarios have been discussed and *** further validate the performance of FL-HMChain,a Convolutional Neural Network-based Federated Learning(FL-CNN-HMChain)model is investigated for medical image *** model achieves better performance compared to the baseline Convolutional Neural Network(CNN),having an average improvement of 4.7%on Area Under Curve(AUC)and 7%on Accuracy(ACC),***,the probability of privacy leakage can be effectively reduced by the blockchain-based parameter transfer mechanism in federated learning between local and global models.

关键词： Blockchain technique federated learning healthcare and medical data collaboration service privacy preservation

Hard-carbon hybrid Li-ion/metal anode enabled by preferred mesoporous uniform lithium growth mechanism

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Journal of Energy Chemistry 2024年第1期88卷 252-259,I0006页

作者： fang Yan Yan Liu Yuan Li Yan Wang Zicen Deng Meng Li Zhenwei Zhu Aohan zhou Ting Li Jingyi qiu Gaoping Cao Shaobo Huang Biyan Wang Hao Zhang School of Chemistry and Biological Engineering University of Science and TechnologyBeijing 100083China Research Institute of Chemical Defense Beijing 100191China College of Physics and Engineering Henan University of Science and TechnologyLuoyang 471023HenanChina National Base for International Science&Technology Cooperation School of ChemistryXiangtan UniversityXiangtan 411105HunanChina Materials and Manufacturing Department Beijing University of TechnologyBeijing 100124China

To achieve high energy density in lithium batteries,the construction of lithium-ion/metal hybrid anodes is a promising *** particular,because of the anisotropy of graphite,hybrid anode formed by graphite/Li metal has low transport kinetics and is easy to causes the growth of lithium dendrites and accumulation of dead Li,which seriously affects the cycle life of batteries and even causes safety ***,by comparing graphite with two types of hard carbon,it was found that hybrid anode formed by hard carbon and lithium metal,possessing more disordered mesoporous structure and lithophilic groups,presents better *** indicate that the mesoporous structure provides abundant active site and storage space for dead *** the synergistic effect of this structure and lithophilic functional groups(–COOH),the reversibility of hard carbon/lithium metal hybrid anode is maintained,promoting uniform deposition of lithium metal and alleviating formation of lithium *** hybrid anode maintains a 99.5%Coulombic efficiency(CE)after 260 cycles at a specific capacity of 500 m Ah/*** work provides new insights into the hybrid anodes formed by carbon-based materials and lithium metal with high specific energy and fast charging ability.

关键词： Hard carbon/Li metal hybrid anode Mesoporous structure Surface oxygen functional group Fast charging Lithium batteries