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Comparison of photosynthesis and antioxidative protection in Sophora moorcroftiana and Caragana maximovicziana under water stress

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Journal of Arid Land 2014年第5期6卷 637-645页

作者： qiqiang guo WenHui ZHANG HuiE LI Ministry of Education Key Laboratory of Environment and Ecology in West China Northwest A&F University Tibet Agricultural and Animal Husbandry College National Key Station for Field Scientific Observation&Experiment

This study aims to investigate the protective roles of photosynthetic characteristics and antioxidative systems in the desiccation tolerance of Sophora moorcroftiana and Caragana maximovicziana as they adapt to arid environments. A variety of physiological and biochemical parameters in the leaves of two Leguminosae species were monitored for 1, 7, 14, 21 and 28 d of drought stress. Soil water content decreased from 38.58% to 7.33% after exposure to 28 d of water stress. The photosynthetic carbon-assimilation rates of the two Leguminosae plants decreased for non-stomatal limitation with processing water stress. The malondialdehyde content and cell membrane relative conductivity of the two species increased significantly from 1 to 21 d and then decreased. S. moorcroftiana showed higher superoxide dismutase and peroxidase activities than C. maximovicziana during the 28 d treatment period. However, the catalase activities and proline content of C. maximovicziana were higher than those of S. moorcroftiana before the water stress treatment reached 21 d. Nine physiological and biochemical parameters were selected to comprehensively evaluate the two species＇ drought-resistance by the membership function values（MFV）. The mean MFV indicated that S. moorcroftiana has a relatively stronger drought defense capability than C. maximovicziana. S. moorcroftiana mainly uses carbon-assimilation rate and osmotic adjustment to combat water deficiency.

关键词： Sophora moorcroftiana Caragana maximovicziana photosynthesis characteristic antioxidative protection drought stress

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Comparative study on the chloroplast genomes of five Larix species from the Qinghai-Tibet Plateau and the screening of candidate DNA markers

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Journal of Forestry Research 2021年第5期32卷 2219-2226页

作者： qiqiang guo Huie Li Zengqiang Qian Jie Lu Weilie Zheng Institute for Forest Resources&Environment of Guizhou/College of Forestry Guizhou UniversityGuiyang 550025People’s Republic of China College of Agriculture Guizhou UniversityGuiyang 550025People’s Republic of China College of Life Sciences Shaanxi Normal UniversityXi’an 710062People’s Republic of China Key Laboratory of Forest Ecology in Tibet Plateau(Tibet Agriculture&Animal Husbandry University) Ministry of EducationNyingchi 860000TibetPeople’s Republic of China Institute of Tibet Plateau Ecology Tibet Agriculture&Animal Husbandry UniversityNyingchi 860000TibetPeople’s Republic of China

Five Larix species(***,***,***,***,and *** ***),have survived on the Qinghai-Tibet Plateau(QTP)under specific climate conditions for *** lack of genomic information seriously hinders research on the evolution,conservation and ecology of these Larix *** this study,complete chloroplast(cp)genomes of the 5 species were assembled and compared based on next generation sequencing technology combined with polymerase chain reaction *** results show that the 5 cp genomes are relatively conservative in size,gene content and arrangement,and border *** analysis showed that the species are closely related as well as to seven other species of the same *** addition,the 5 cp genomes contained few simple sequence repeats and relatively low nucleotide variability;thus,12 candidate polymorphic cp DNA markers will be helpful for further research on relevant population *** results will provide valuable genetic information for the conservation,evolution and ecology of these species and their relatives.

关键词： Larix species Plastid genome Microsatellite Phylogenetic analysis Qinghai-tibet plateau

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Geographical differences of leaf traits of the endangered plant Litsea coreana *** and its relationship with climate

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Journal of Forestry Research 2023年第1期34卷 125-135页

作者： Gangyi Yuan qiqiang guo Yaqin Zhang Qin Gui Na Xie Siqiong Luo Institute for Forest Resources and Environment of Guizhou Key Laboratory of Forest Cultivation in Plateau Mountain of Guizhou ProvinceCollege of ForestryGuizhou UniversityGuiyang 550025People’s Republic of China

Seventeen morphological and anatomical characteristics of the leaves were selected from five natural populations to explore the variation in leaf traits of Lits ea core ana *** and the effects of geographical environment on these *** analysis of variance,multiple comparisons,principal component analysis(PCA),and correlation analysis were conducted to explore the variations within and between populations and their correlation with geographical and climatic *** differences in the 17 leaf traits were observed within and among *** average,the relative contribution of within population variation to total variation was 24.8%,which was lower than among population variation(54.6%).The average differentiation coefficient of the traits was 65.8%,and the average coefficient of variation 11.8%,ranging from6.7%for main vein thickness to 21.4%for petiole *** PC A results showed that morphological characteristics were divided into two categories,and the level of variation was greater than that of leaf *** of the leaf traits were significantly correlated with geography and climate and showed a gradual variation with longitude,latitude,and *** areas with high temperatures,less rainfall,and strong seasonal rainfall,the leaves are larger,longer and *** study shows that variations in leaf traits of *** *** mainly come from variations among *** level of trait differentiation among populations is high and the level of variation within populations *** findings help further understand leaf morphological characteristics of this species and can provide a valuable reference for the protection and sustainable utilization of this natural resource.

关键词： Litsea coreana var.sinensis Climate Leaf trait variations Natural populations Endangered species

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Analysis of genetic diversity and prediction of Larix species distribution in the Qinghai–Tibet Plateau,China

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Journal of Forestry Research 2023年第3期34卷 705-715页

作者： qiqiang guo Huie Li Weilie Zheng Jinwen Pan Jie Lu Jiangrong Li Yu Zheng Institute for Forest Resources&Environment of Guizhou Key Laboratory of Forest Cultivation in Plateau Mountain of Guizhou ProvinceGuizhou UniversityGuiyang 550025People’s Republic of China College of Forestry Guizhou UniversityGuiyang 550025People’s Republic of China College of Agriculture Guizhou UniversityGuiyang 550025People’s Republic of China Institute of Tibet Plateau Ecology Tibet Agriculture and Animal Husbandry UniversityNyingchi Tibet 860000People’s Republic of China Key Laboratory of Forest Ecology in Tibet Plateau(Tibet Agriculture and Animal Husbandry University) Ministry of EducationNyingchi Tibet 860000People’s Republic of China Plant Research Center Chengdu Botanical GardenChengdu 610083People’s Republic of China

Larix resources in the Qinghai-Tibet Plateau have important ecological and economic ***,the lack of genetic diversity background and related research hinders the development of conservation *** this study,genetic diversity and distribution of fi ve Larix species were *** 19 polymorphic microsatellite markers to study 272 representative individuals from 13 populations,the results show low genetic diversity at the population level,with variation explained mainly by diff erentiation among *** Larix populations were classifi ed into two clades,one formed by eight populations,including three of the species in this study,***,***,and *** *** other clade consists of fi ve populations,including the other two species in this study,*** thii and *** distance of the species was aff ected by geographical isolation and genetic diversity was mainly aff ected by *** area suitable for Larix *** during the Last Glacial Maximum compared to the current distribution according to the niche model,but should increase in future climate scenarios(2050s),expanding westward along the *** results provide an important scientifi c basis for the development of conservation strategies and further the sustainable utilization of Larix resources in the Qinghai-Tibet Plateau.

关键词： Larix spp. Genetic diversity Phylogenetic relationship Genetic structure Ecological niche modeling Qinghai-Tibet Plateau

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Unraveling the nexus between cellular senescence and malignant transformation:a paradigm shift in cancer research

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Cancer Biology & Medicine 2024年第7期21卷 541-546页

作者： Xiaoyu Song Xiyan Liu qiqiang guo Hongde Xu Liu Cao The College of Basic Medical Science Health Sciences InstituteChina Medical UniversityShenyang 110122China Key Laboratory of Cell Biology of Ministry of Public Health Key Laboratory of Medical Cell Biology of Ministry of EducationKey Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors of Ministry of EducationLiaoning Province Collaborative Innovation Center of Aging Related Disease Diagnosis and Treatment and PreventionChina Medical UniversityShenyang 110122China

Cellular senescence, a natural process wherein cells cease division and undergo irreversible growth arrest, has long captivated the curiosity of scientists because of its many implications in aging and disease. Recent research has shed light on the nexus between cellular senescence and malignant transformation, thus leading to a paradigm shift in understanding cancer development and progression.

关键词： malignant cancer transformation

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草海国家级自然保护区森林群落优势种群数量动态及物种多样性研究

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西北植物学报 2021年第7期41卷 1229-1239页

作者：袁刚毅郭其强杨瑞贵州大学林学院贵州省森林资源与环境研究中心贵阳550025

该研究以草海国家级自然保护区森林群落乔木树种为依据进行聚类分析,将森林划分为4种群落类型,分别为云南松林、云南松-桤木林、桤木-滇杨林、桤木-华山松林。采用空间代替时间的方法,分析不同种群优势树种的年龄结构、存活曲线及静态... 详细信息

该研究以草海国家级自然保护区森林群落乔木树种为依据进行聚类分析,将森林划分为4种群落类型,分别为云南松林、云南松-桤木林、桤木-滇杨林、桤木-华山松林。采用空间代替时间的方法,分析不同种群优势树种的年龄结构、存活曲线及静态生命表,应用时间预测模型对4种群落优势树种的发展趋势进行预测,并分析群落物种多样性,为研究区森林保护及可持续经营提供理论依据。结果表明:(1)云南松林和云南松-桤木林优势树种径级结构均呈现偏正态分布,衰退趋势明显,桤木-滇杨林、桤木-华山松林呈偏倒“J”型,衰退趋势相对较弱。(2)静态生命表及存活曲线分析表明,研究区内森林群落不同种群均出现不稳定状态,存活曲线不同程度偏离DeeveyⅢ型曲线。(3)时间序列分析表明研究区内经过未来2、4、6龄级时间后各种群数量出现增加,但中幼龄个体不断减少,老龄个体先增多后减少,如果不加以抚育管理,种群将不可避免走向衰退。(4)研究区内林下共有植物种类35科70属83种,其中灌木层植物17科23属29种,草本层植物21科48属54种。在3种群落类型中,物种丰富度指数(S)为草本层>灌木层,Pielou指数(Jsw)为灌木层>草本层,而桤木-华山松林则呈现相反趋势,物种多样性存在明显差异。

关键词：草海国家级自然保护区优势种群结构存活曲线时间序列预测物种多样性

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青藏高原特有种砂生槐的核心种质构建

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植物生理学报 2022年第6期58卷 1133-1144页

作者：杨兰李慧娥郭其强贵州大学农学院贵阳550025 贵州大学贵州省森林资源与环境研究中心贵阳550025

以300份砂生槐代表样本为研究对象,结合砂生槐形态特征、生物碱含量、抗旱等级与分子标记,对砂生槐资源进行核心种质构建。结果显示:用作分子标记的20对SSR引物多态百分率为89.33%,6个形态特征(冠幅、基径、分蘖数、株高、花序长、种子... 详细信息

以300份砂生槐代表样本为研究对象,结合砂生槐形态特征、生物碱含量、抗旱等级与分子标记,对砂生槐资源进行核心种质构建。结果显示:用作分子标记的20对SSR引物多态百分率为89.33%,6个形态特征(冠幅、基径、分蘖数、株高、花序长、种子数)和1种生物碱(苦参碱)含量的变异系数均在30%以上,但砂生槐资源的6个抗旱相关指标的变异系数低于10%。因此,将形态特征与生物碱含量进行10级分类,与SSR分型结果一起,采用位点优先取样策略构建核心种质,最终筛选出38份核心种质。进一步对所筛选出的核心种质相对于原始种质进行评价与确认,所构建的38份核心种质在形态特征、生物碱含量、抗旱性、地区分布及分子水平上均能够代表原始种质的遗传多样性。

关键词：砂生槐核心种质位点优先取样遗传多样性种质资源

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贵州威宁红花油茶的叶绿体基因组特征分析

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园艺学报 2020年第4期47卷 779-787页

作者：李倩郭其强高超李慧娥贵州大学农学院园艺系贵阳550025 贵州大学贵州省森林资源与环境研究中心贵阳550025

对威宁红花油茶(Camellia weiningensis)的叶片高通量测序数据进行叶绿体全基因组的组装和注释分析。其叶绿体全基因组长为156490 bp,包含典型的四分体结构,序列已登录GenBank(MK820035)。叶绿体全基因组比较分析表明,与其他山茶属物种... 详细信息

对威宁红花油茶(Camellia weiningensis)的叶片高通量测序数据进行叶绿体全基因组的组装和注释分析。其叶绿体全基因组长为156490 bp,包含典型的四分体结构,序列已登录GenBank(MK820035)。叶绿体全基因组比较分析表明,与其他山茶属物种一样,其结构与基因排序均保守,但在基因组的反向重复区边界处表现出明显区别。简单重复序列(SSR)分析表明,全基因组仅包含51个单核苷酸重复的SSR,且仅为A/T重复类型。系统发育分析表明,威宁红花油茶与腾冲红花油茶亲缘关系最近,但其所处分支包含的4个山茶属物种的分组与传统分类不一致。

关键词：威宁红花油茶叶绿体基因组系统发育

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西藏东南部急尖长苞冷杉蒸腾耗水及其驱动因子分析

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南京林业大学学报（自然科学版） 2021年第6期45卷 151-158页

作者：李江荣郭其强郑维列西藏农牧学院高原生态研究所西藏高原森林生态教育部重点实验室西藏林芝高山森林生态系统国家野外科学观测研究站西藏林芝860000

【目的】探讨不同径级急尖长苞冷杉个体蒸腾耗水规律,并分析其与主要气象因子的相关性,为明确西藏亚高山区林木生长规律提供重要参考。【方法】应用树干液流测定系统在西藏色季拉山天然急尖长苞冷杉林内对8株不同径级急尖长苞冷杉个体... 详细信息

【目的】探讨不同径级急尖长苞冷杉个体蒸腾耗水规律,并分析其与主要气象因子的相关性,为明确西藏亚高山区林木生长规律提供重要参考。【方法】应用树干液流测定系统在西藏色季拉山天然急尖长苞冷杉林内对8株不同径级急尖长苞冷杉个体进行蒸腾耗水的连续测定,并同步监测5个气象因子。【结果】急尖长苞冷杉树干液流速率日变化为单峰曲线,7月、8月和9月8株样木平均液流速率分别为137.09、142.71和184.43 g/h,日活动时间为10:00—22:00,其峰值出现在16:00左右。急尖长苞冷杉树干蒸腾耗水速率与大气温度和光合有效辐射呈极显著正相关关系,与饱和水汽压差显著正相关,与大气湿度极显著负相关,与风速无关;蒸腾耗水与各因子相关系数绝对值排序为大气湿度>大气温度>光合有效辐射>饱和水汽压差。急尖长苞冷杉边材面积和日蒸腾耗水量均与胸径呈极显著的二次曲线关系,决定系数分别为0.999和0.937。【结论】急尖长苞冷杉蒸腾耗水日变化呈"N"形变化,受大气温湿度和光合有效辐射等环境因子影响显著;随胸径增加,单株日蒸腾耗水量迅速升高。

关键词：急尖长苞冷杉蒸腾耗水驱动因子西藏东南部

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二元合金在双氧化剂中的腐蚀动力学实例分析

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腐蚀科学与防护技术 2016年第5期28卷 429-434页

作者：吴祥锋郭启强沈俊牛焱中国科学院金属研究所沈阳110016

提出了一种描述在双氧化剂(X-Y)中、且生成有外氧化膜(AY_n)的情况下,二元合金(A-B)的腐蚀模型,并利用该模型对Fe-Cr合金的腐蚀动力学实例进行了分析。表明该模型的预测可信,而氧化剂在合金中的实际扩散路径复杂且尚难预见是导致内氧化... 详细信息

提出了一种描述在双氧化剂(X-Y)中、且生成有外氧化膜(AY_n)的情况下,二元合金(A-B)的腐蚀模型,并利用该模型对Fe-Cr合金的腐蚀动力学实例进行了分析。表明该模型的预测可信,而氧化剂在合金中的实际扩散路径复杂且尚难预见是导致内氧化区厚度的理论计算值与实验测定值之间存在较大偏差的主要原因。

关键词： Fe-Cr合金双氧化剂动力学

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