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基于PLC的IGCT测试夹具控制系统的研制

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电力电子技术 2024年第9期58卷 118-120页

作者：肖秦梁乔宇于庆饶琼西安电力电子技术研究所测试设备开发室陕西西安710119

该系统以S7-200 SMART型的可编程逻辑控制器(PLC)为控制核心,配合使用压控恒流源、比例阀、压力传感器、温度控制器、安全光栅等其他硬件设施,可实现集成门极换流晶闸管(IGCT)测试夹具的动作控制、压力显示、测试及安全互锁等功能。对... 详细信息

该系统以S7-200 SMART型的可编程逻辑控制器(PLC)为控制核心,配合使用压控恒流源、比例阀、压力传感器、温度控制器、安全光栅等其他硬件设施,可实现集成门极换流晶闸管(IGCT)测试夹具的动作控制、压力显示、测试及安全互锁等功能。对比例阀的控制信号进行了精确计算与仿真。基于STEP7软件平台,对PLC的主要软件程序进行了设计,将夹具的控制系统部署到IGCT器件的测试系统中去,经过三年多的现场实际应用,发现各项功能都能够实现且运行高效稳定,取得了良好的效果。

关键词：可编程逻辑控制器比例阀测试夹具

Effect of Bacillus thuringiensis on Habrobracon hebetor during combined biological control of Plodia interpunctella

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Insect Science 2009年第5期16卷 409-416页

作者： Akinkurolere Rotimi Oluwafemi qiong rao Xi-Qiao Wang Hong-Yu Zhang State Key Laboratory of Agricultural Microbiology Institute of Urban Pests and Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory College of Plant Science and Technology Huazhong Agricultural University Wuhan Wuhan Service Station for Vegetable Crops Wuhan China Laboratory of Food Storage Technology Biology Department Federal University of Technology Akure Nigeria

The suitability of combining microbial pesticides and an insect parasitoid for pest management of stored cereal in China was evaluated using laboratory assays. For this purpose, interactions between Bacillus thuringiensis （Bt）, Bt-intoxicated host larvae and the parasitoid Habrobracon hebetor （Say）（Hymenoptera： Braconidae） were tested against Plodia interpunctella Hubner （Lepidoptera： Pyralidae）. Bt or H. hebetor alone caused 41.67% and 35.35% P interpunctella larval mortality respectively. The Bt- parasitoid combined treatment significantly increased mortality ofP interpunctella （86%）. Progeny development of H. hebetor was dependent upon its susceptibility to Bt. Fewer parasitoids emerged from Bt-parasitoid combined treatment than in non-Bt treatments. However, since Bt did not prevent parasitoid development, a combined treatment with Bt and parasitoid release could produce better protection against P. interpunctella than either treatments when used singly, because their lethal effects were additive to each other.

关键词： Bacillus thuringiensis Habrobracon hebetor pest management Plodia interpunctella

Diversity of secondary endosymbionts among different putative species of the whitefly Bemisia tabaci

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Insect Science 2013年第2期20卷 194-206页

作者： Xiao-Li Bing Yong-Ming Ruan qiong rao Xiao-Wei Wang Shu-Sheng Liu Ministry of Agriculture Key Laboratory of Agricultural Entomology Institute of Insect Sciences Zhejiang University Hangzhou College of Chemistry and Life Science Zhejiang Normal University Jinhua Zhejiang Province China

Endosymbionts are important components of arthropod biology. The white- fly Bemisia tabaci （Gennadius）（Hemiptera： Aleyrodidae） is a cryptic species complex composed of 〉28 putative species. In addition to the primary endosymbiont Portiera aley- rodidarum, six secondary endosymbionts （S-endosymbionts）, Hamiltonella, Rickettsia, Wolbachia, Cardinium, Arsenophonus and Fritschea, have been identified in B. tabaci thus far. Here, we tested five of the six S-endosymbiont lineages （excluding Fritschea） from 340 whitely individuals representing six putative species from China. Harniltonella was detected only in the two exotic invaders, Middle East-Asia Minor 1 （MEAM 1） and Mediter- ranean （MED）. Rickettsia was absent in Asia II 1 and MED, scarce in Asia II 3 （13%）, but abundant in Asia II 7 （63.2%）, China 1 （84.7%） and MEAM1 （100%）. Wolbachia, Cardinium and Arsenophonus were absent in the invasive MEAM1 and MED but mostly abundant in the native putative species. Furthermore, phylogenetic analyses revealed that some S-endosymbionts have several clades and different B. tabaci putative species can harbor different clades of a given S-endosymbiont, demonstrating further the complexity of S-endosymbionts in B. tabaci. All together, our results demonstrate the variation and diversity of S-endosymbionts in different putative species ofB. tabaci, especially between invasive and native whiteflies.

关键词： Arsenophonus Cardinium Hamiltonella Rickettsia whitefly Wolbachia

Diversity and Genetic Differentiation of the Whitefly Bemisia tabaci Species Complex in China Based on mtCOI and cDNA-AFLP Analysis

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Journal of Integrative Agriculture 2012年第2期11卷 206-214页

作者： GUO Xiao-jun rao qiong ZHANG Fan LUO Chen ZHANG Hong-yu GAO Xi-wu Department of Entomology China Agricultural University Beijing 100193 P.R.China Institute of Plant and Environmental Protection Beijing Academy of Agriculture and Forestry Sciences Beijing 100097 P.R. China College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 PR. China

The whitefly Bemisia tabaci are considered as a taxonomically complex that contained some destructive *** of the most prevalent cryptic species are *** Middle East-Asia Minor 1（MEAM1）and Mediterranean（MED）.In an extensive field survey of the *** complex present throughout part of China from 2004 to 2007,we obtained 93 samples of *** from 22 *** determined that these Chinese haplotypes included 2 invasive species（MEAM1 and MED）,and 4 indigenous cryptic species（Asia II 1,Asia II 3,China 3 and Asia II 7）by sequencing mitochondrial cytochrome oxidose one gene（mtCOI）.The diversity and genetic differentiation of a subset of 19 populations of *** were studied using cDNA amplified fragment length polymorphism（AFLP）.Prior to 2007,MEAM1 was a dominant species in many provinces in *** 2007,MED was dominant in 11 *** invasive and indigenous species were simultaneously found in some *** species of *** were found in six provinces in southern *** and MEAM1 have broad ranges of host plants,and indigenous species appeared to have much narrower host *** Asia II 3 samples were found on cotton except one on *** 3 has more host plants than Asia II *** samples of China 3 were collected from sweet potato,Japanese hop,squash and cotton.A total of 677 reproducible bands amplified with 5 AFLP primer combinations were *** highest proportion of polymorphic bands was 98.7% and the lowest was 91.9%.Unweighted pair-group method analysis indicated that the clustering was independent of the different *** showed the lowest degree of similarity than the other *** data indicate that both MEAM1and MED were rapidly established in China.

关键词： Bemisia tabaci mtCOI cDNA-AFLP diversity whitefly

Genomic and transcriptomic analyses reveal metabolic complementarity between whiteflies and their symbionts

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Insect Science 2022年第2期29卷 539-549页

作者： Dan-Tong Zhu qiong rao Chi Zou Fei-Xue Ban Juan-Juan Zhao Shu-Sheng Liu Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects Institute of Insect SciencesZhejiang UniversityHangzhou310029 China School of Agriculture and Food Science Zhejiang Agriculture and Forestry UniversityLin'anHangzhou311300 China

Nutritional mutualism between insects and symbiotic bacteria is *** various sap-feeding whitefly species within the Bemisia tabaci complex associate with the same obligate symbiont(Portiera)and multiple secondary *** is often assumed that some of the symbionts residing in the whiteflies play crucial roles in the nutritional physiology of their insect *** effort has been made to understand the functions of the whitefly symbionts,the metabolic complementarity offered by these symbionts to the hosts is not yet well *** examined two secondary symbionts,Arsenophonus and Wolbachia,in two species of the *** whitefly complex,provisionally named as Asia II 3 and China *** sequence analyses revealed that Arsenophonus and Wolbachia retained genes responsible for the biosynthesis of B *** then conducted transcriptomic surveys of the bacteriomes in these two species of whiteflies together with that in another species named MED of this whitefly complex previously *** analyses indicated that several key genes in B vitamin syntheses from the three whitefly species were *** findings suggest that,similar to another secondary symbiont Hamiltonella,Arsenophonus and Wolbachia function in the nutrient provision of host *** phylogenetically distant species of symbionts are associated with their respective hosts,they have evolved and retained similar functions in biosynthesis of some B *** metabolic complementarity between whiteflies and symbionts represents an important feature of their coevolution.

关键词： symbiont B vitamins complement evolution whitefly

Sequencing and comparison of the Rickettsia genomes from the whitefly Bemisia tabaci Middle East Asia Minor I

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Insect Science 2016年第4期23卷 531-542页

作者： Dan-Tong Zhu Wen-Qiang Xia qiong rao Shu-Sheng Liu Murad Ghanim Xiao-Wei Wang Ministry of Agriculture Key Laboratory of Agricultural Entomology Institute of Insect Sciences Zhejiang University Hangzhou China Department of Entomology Agricultural Research Organization The Volcani Center Bet Dagan Israel School of Agriculture and Food Science Zhejiang Agriculture and Forestry University Lin'an Hangzhou China

The whitefly, Bemisia tabaci, harbors the primary symbiont ＇Candidatus Portiera aleyrodidarum＇ and a variety of secondary symbionts. Among these secondary symbionts, Rickettsia is the only one that can be detected both inside and outside the bacteriomes. Infection with Rickettsia has been reported to influence several aspects of the whitefly biology, such as fitness, sex ratio, virus transmission and resistance to pesticides. However, mechanisms underlying these differences remain unclear, largely due to the lack of genomic information of Rickettsia. In this study, we sequenced the genome of two Rickettsia strains isolated from the Middle East Asia Minor 1 （MEAM1） species of the B. tabaci complex in China and Israel. Both Rickettsia genomes were of high coding den- sity and AT-rich, containing more than 1000 coding sequences, much larger than that of the coexisted primary symbiont, Portiera. Moreover, the two Rickettsia strains isolated from China and Israel shared most of the genes with 100% identity and only nine genes showed sequence differences. The phylogenetic analysis using orthologs shared in the genus, inferred the proximity of Rickettsia in MEAM1 and Rickettsia bellii. Functional analysis revealed that Rickettsia was unable to synthesize amino acids required for complementing the whitefly nutrition. Besides, a type IV secretion system and a number of virulence- related genes were detected in the Rickettsia genome. The presence of virulence-related genes might benefit the symbiotic life of the bacteria, and hint on potential effects of Rickettsia on whiteflies. The genome sequences of Rickettsia provided a basis for further understanding the function of Rickettsia in whiteflies.

关键词： Bemisia tabaci comparison endosymbiont genome Rickettsia symbiosis

Characterisation of Neonicotinoid and Pymetrozine Resistance in Strains of Bemisia tabaci(Hemiptera:Aleyrodidae)from China

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Journal of Integrative Agriculture 2012年第2期11卷 321-326页

作者： rao qiong XU Yong-hua LUO Chen ZHANG Hong-yu Christopher M Jones Greg J Devine KevinGorman Ian Denholm College of Plant Science and Technology Huazhong Agricultural University Wuhan 430070 P.R.China Institute of Plant and Environmental Protection Beijing Academy of Agriculture ＆ Forestry Sciences Beijing 100097 P.R.China Plant and Invertebrate Ecology Department Rothamsted Research Harpenden Herts .4L5 2JQ United Kingdom Zhejiang Chemical Industry Research Institute Zhejiang Branch of National Pesticide R＆D South Center Hangzhou 310023 P.R. China

Four strains of the Q biotype and one of the B biotype of the whitefly Bemisia tabaci collected from China were characterised for resistance to four neonicotinoid insecticides and pymetrozine.Q biotype strains showed moderate to strong resistance to imidacloprid,thiamethoxam and acetamiprid,but little or no cross-resistance to *** to neonicotinoids was consistently associated with resistance to pymetrozine,despite the latter having a distinct（though unresolved）mode of *** single B biotype strain proved largely susceptible to all the insecticides *** in the Q biotype strains was associated with over-expression of a cytochrome P450 monooxygenase gene,CYP6CM1,whose substrate specificity presumably accounts for the observed cross-resistance profiles.

关键词： P450 cross-resistance pymetrozine imidacloprid thiamethoxam acetamiprid dinotefuran neonicotinoids

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昆虫专性内共生细菌及其基因组研究进展

微生物学报 2014年第7期54卷 728-736页

作者：饶琼吴慧明浙江农林大学农业与食品科学学院浙江临安311300

昆虫专性内共生细菌是一类与宿主昆虫长期协同进化的共生微生物,在许多昆虫体内均有发现,主要存在于昆虫特化的器官(含菌体)内,以垂直传播的方式由母系遗传。专性内共生细菌与昆虫的生存、繁殖以及进化等方面息息相关,其主要功能是为宿... 详细信息

昆虫专性内共生细菌是一类与宿主昆虫长期协同进化的共生微生物,在许多昆虫体内均有发现,主要存在于昆虫特化的器官(含菌体)内,以垂直传播的方式由母系遗传。专性内共生细菌与昆虫的生存、繁殖以及进化等方面息息相关,其主要功能是为宿主提供必需氨基酸等营养物质。因其长期生活在宿主细胞内处于封闭的高营养的环境中,其基因组的特征与普通细菌基因组有很大区别,包括基因组大小、GC含量、基因缺失等方面。通过对共生细菌基因水平上的深入研究,有助于理解专性内共生细菌在宿主昆虫协同进化过程中的作用。目前,昆虫内共生细菌基因的生物学功能、内共生细菌之间以及内共生细菌与宿主之间的互作机制还不是很清楚,有待进一步的研究和探索。

关键词：共生细菌基因组协同进化营养互作

基于激励相容视角的中央区域激励政策评价研究:以海南省为例

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海南大学学报（人文社会科学版） 2013年第2期31卷 95-102页

作者：蒋国洲饶琼娟海南大学经济与管理学院海南海口570228 海口市文化广电出版体育局海南海口570208

运用经济学中的激励相容原则,构建了中央地方激励相容关系的条件(要求),并以中央两个激励过程都享受了中央激励政策的海南为例进行了检验。依此得出结论:"自上而下"以政策优惠为主要特征的区域激励政策有较强的激励功能,但同时也显示出... 详细信息

运用经济学中的激励相容原则,构建了中央地方激励相容关系的条件(要求),并以中央两个激励过程都享受了中央激励政策的海南为例进行了检验。依此得出结论:"自上而下"以政策优惠为主要特征的区域激励政策有较强的激励功能,但同时也显示出许多激励不相容;"自下而上"式的优惠政策和激励措施更能满足激励相容的条件,达到更好的激励相容的效果。

关键词：激励相容央地关系优惠政策先行先试权