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Nano Au/TiO_2 hollow microsphere membranes for the improved sensitivity of detecting specific DNA sequences related to transgenes in transgenic plants

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Science China Chemistry 2008年第11期51卷 1066-1073页

作者： ZHANG YongChun, YANG Tao, ZHOU Na, ZHANG Wei & JIAO Kui College of Chemistry and Molecular Engineering, qingdao University of Science and Technology, qingdao 266042, China 1. College of Chemistry and Molecular Engineering Qingdao University of Science and Technology Qingdao 266042 China

Gold nanoparticles (nano Au)/titanium dioxide (TiO2) hollow microsphere membranes were prepared on the carbon paste electrode (CPE) for enhancing the sensitivity of DNA hybridization detection. The immobilization of nano Au and TiO2 microsphere was investigated with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The hybridization events were monitored with EIS us-ing [Fe(CN)6]3-/4- as indicator. The sequence-specific DNA of the 35S promoter from cauliflower mosaic virus (CaMV35S) gene was detected with this DNA electrochemical sensor. The dynamic detection range was from 1.0×10-12 to 1.0×10-8 mol/L DNA and a detection limit of 2.3×10-13 mol/L could be ob-tained. The polymerase chain reaction (PCR) amplification of the terminator of nopaline synthase (NOS) gene from the real sample of a kind of transgenic soybean was also satisfactorily detected.

关键词： Au nanoparticle TiO_2 hollow microsphere DNA electrochemical biosensor CaMV35S gene NOS gene

Nano-composite Particles Synthesized by a Hydrogen Arc Plasma

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Journal of Materials Science & Technology 1997年第4期13卷 315-317页

作者： Zuolin CUI Zhikun ZHANG Chuncheng HAO Lifeng DONG Zhaoguo MENG and Liyan YU(Research Center of Nanostructured Materials, qingdao Institute of Chemical Technology qingdao 266042, China) Research Center of Nanostructured Materials Qingdao Institute of Chemical Technology Qingdao 266042 China

Nano-composite particles can be synthesized by a hydrogen arc plasma method, which possesses the advantages of high productivity, controllable size distribution and low electric energy consumption comparing with conventional gas condensation method. With this method, not only the nanoparticles of metals and alloys, but also the nano-composite particles with shell structure can be synthesized. The microstructures, compositions and the formation mechanism of the nano composite particles were studied

关键词： Nano-composite Particles Synthesized by a Hydrogen Arc Plasma Arc

Stereocontrol during the radical polymerization of methyl methacrylates with combined Lewis acids:Aluminium trichloride(AlCl_3) and iron dichloride tetrahydrate

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Science China Chemistry 2010年第5期53卷 1141-1147页

作者： SANG Qian,FENG Ying ,DONG XiuCai & ZHAO JiRuo Key Laboratory of Rubber-Plastics,Ministry of Education qingdao University of Science and Technology,qingdao 266042,China 1. Key Laboratory of Rubber-Plastics Ministry of Education Qingdao University of Science and Technology Qingdao 266042 China

The radical polymerization of methyl methacrylate(MMA) was carried out in the presence of combined Lewis acids of the AlCl3-FeCl2 *** with the polymerization produced in the presence of single Lewis acids,AlCl3 or FeCl2,the MMA polymerization in the presence of AlCl3-FeCl2 composite in CHCl3 or 1-butanol produced a polymer with a higher isotacticity and in toluene produced a polymer with a much higher isotacticity(mm=50%) .The molecular weight and polydispersity of PMMA in the presence of Lewis acids were similar with those in the absence of Lewis acids,although Lewis acids decelerate the polymerization of *** effects of the Lewis acids were greater in a solvent with a lower polarity.A possible stereocontrol mechanism of the polymerization was *** Lewis acid composite of AlCl3-FeCl2 readily formed a complex with growing *** complexes possessed apparent bulkiness that changes the direction of monomer addition to the growing radical center.

关键词： MMA radical polymerization tacticity Lewis acid stereocontrol polymerization

Studies of Laser-induced-MOCVDZinc Oxide Films

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Chemical Research in Chinese Universities 1994年第3期10卷 240-243页

作者： REN Peng-cheng, TAN Zhong-ke and LUO Wen-xiu(Ceiiter for Functional Materials Research, qingdao Institute of Chemical Technology,qingdao,266042)

hin films of ZnO were prepared using the double photobeams ultraviolet laserinduced-metallorganic chemical vapour deposition (MOCVD)technique. The struc-ture and transparent photoconductive properties of these films were investigated us-ing X-ray diffraction (XRD) , reflecting electron diffraction (RED) .scanning elec-tron microscopy (SEM) and ultraviolet visible absorption spectrometry (UV) .Theexperiments showed that the technique produced superior quality films of polycrys-tal ZnO_(1-x) (the O-vacancies in the ZnO lattice) , and possessed higher depositionrate, lower growth temperature conipared with CVD or MOCVD technique and thethin films had far better transparent photoconductive properties than tliose grownby the conventional CVD or MOCVD technique.

关键词： Double-photobeams ultraviolet laser induction . Metallorganic chemicalvapour tleposition(MOCVD) Zinc oxide film

Improved electrochemical performances of polyaniline nanotubes-poly-L-lysine composite for label-free impedance detection of DNA hybridization

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Science China Chemistry 2010年第6期53卷 1371-1377页

作者： YANG Tao,JIANG Chen,ZHANG Wei & JIAO Kui Key Laboratory of Eco-chemical Engineering,Ministry of Education College of Chemistry and Molecular Engineering,qingdao University of Science and Technology,qingdao 266042,China 1. Key Laboratory of Eco-chemical Engineering Ministry of Education College of Chemistry and Molecular Engineering Qingdao University of Science and Technology Qingdao 266042 China

A sensitive label-free DNA hybridization biosensing platform was fabricated based on the synergistic effect of polyaniline nanotubes (PANInt) and poly-L-lysine (pLys).The composite of pLys and PANInt was coated onto the carbon paste electrode (CPE) to form a uniform and very stable nanocomposite *** pLys in the composite film not only acts as a membrane to retain good electron transfer capability of PANInt even at physiological pH,but also possesses fine biocompatibility for *** probes with negatively charged phosphate groups were readily linked to the positively charged pLys surface due to the strong electrostatic *** synergistic effect of PANInt and pLys could significantly enhance the sensitivity of DNA hybridization *** phosphinothricin acetyltransferase (PAT) gene fragment from transgenic corn and the polymerase chain reaction amplification of the terminator of nopaline synthase gene from the real sample of a kind of transgenic soybean were detected by this DNA electrochemical biosensor via label-free impedance *** stable composite gives convenient permselectivity properties as a transducer material for the design of modern electrochemical impedance biosensor using [Fe(CN)6]3-/4as an indicator.

关键词： poly-L-lysine polyaniline nanotubes DNA electrochemical biosensor PAT gene NOS gene

Adsorption of zinc on manganite(γ-MnOOH): particle concentration effect and adsorption reversibility

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Journal of Environmental Sciences 2004年第4期16卷 627-630页

作者： QIN Yan-wen1, PAN Gang 1,2,, ZHANG Ming-ming1, LI Xian-liang1 (1. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China. 2. qingdao University of Science & Technology, qingdao 266042, China) State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China.

The adsorption and desorption processes of Zn(Ⅱ) on γ-MnOOH as a function of particle concentrations(C p) were studied. An obvious C p effect was observed in this adsorption system. The degree of adsorption hysteresis increased greatly with the increasing of C p, indicating that the extent of the real metastable-equilibrium states deviating from the ideal equilibrium state was enhanced with the increasing of C p. The C p-reversibility relationship confirmed the metastable-equilibrium adsorption(MEA) inequality(Pan, 1998a), which was the core formulation of the MEA theory. Because the MEA inequality was based on the basic hypothesis of MEA theory that adsorption density Γ is not a state variable, the C p-reversibility relationship gave indirect evidence to the basic hypothesis of MEA theory.

关键词： zinc γ-MnOOH adsorption reversibility

Approximately optimal tracking control for discrete time-delay systems with disturbances

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Progress in Natural Science:Materials International 2008年第2期18卷 225-231页

作者： Gongyou Tang a,,Huiying Sun b,Haiping Pang c a College of Information Science and Engineering,Ocean University of China,qingdao 266100,China b College of Information and Electrical Engineering,Shandong University of Science and Technology,qingdao 266510,China c College of Automation and Electronic Engineering,qingdao University of Science and Technology,qingdao 266042,China College of Information Science and Engineering Ocean University of China Qingdao 266100 China College of Information and Electrical Engineering Shandong University of Science and Technology Qingdao 266510 China College of Automation and Electronic Engineering Qingdao University of Science and Technology Qingdao 266042 China

Optimal tracking control (OTC) for discrete time-delay systems affected by persistent disturbances with quadratic performance index is considered. By introducing a sensitivity parameter,the original OTC problem is transformed into a series of two-point boundary value (TPBV) problems without time-advance or time-delay terms. The obtained OTC law consists of analytic feedforward and feedback terms and a compensation term which is the sum of an infinite series of adjoint vectors. The analytic feedforward and feedback terms can be found by solving a Riccati matrix equation and two Stein matrix equations. The compensation term can be obtained by using an iteration formula of the adjoint vectors. Observers are constructed to make the approximate OTC law physically realizable. A simulation example shows that the approximate approach is effective in tracking the reference input and robust with respect to exogenous persistent disturbances.

关键词： Discrete systems Time-delay systems Disturbances Optimal control Tracking control

Preparation, characterization, viscosity and thermal conductivity of CaCO_3 aqueous nanofluids

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Science China(Technological Sciences) 2010年第2期53卷 360-368页

作者： ZHU HaiTao1, LI ChangJiang2, WU DaXiong1, ZHANG CanYing1 & YIN YanSheng2 1 College of Materials Science and Engineering, qingdao University of Science and Technology, qingdao 266042, China 2 Department of Materials, Ocean University of China, qingdao 266003, China 1. College of Materials Science and Engineering Qingdao University of Science and Technology Qingdao 266042 China2. Department of Materials Ocean University of China Qingdao 266003 China

CaCO3 aqueous nanofluids were prepared by dispensing aqueous CaCO3 paste into distilled water under ultrasonic vibration. The actual microstructures of the CaCO3 nanofluids with different particle volume fractions were characterized by freeze etching replication transmission electron microscopy (FERTEM). Thermal conductivity and rheological behavior of the nanofluids were measured by standard analyzers. The results show that CaCO3 paste as raw material for nanofluids is advantageous to reducing aggregation of primary nanoparticles. The effective viscosities and effective thermal conductivities of the CaCO3 nanofluids are related to the aggregates of nanoparticles and can be well predicted by the modified Krieger & Dougherty formula and the modified Hamilton & Crosser model, respectively.

关键词： CaCO3 nanofluid thermal conductivity viscosity microstructure

A thin-layer spectroelectrochemical study of 3,3',5,5'-tetramethylbenzidine at SnO_2:F film optically transparent electrode

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Science China Chemistry 2004年第4期47卷 267-275页

作者： JIAO Kui1, YANG Tao2 & NIU Shuyan1,2 1. College of Chemistry and Molecular Engineering, qingdao University of Science and Technology, qingdao 266042, China 2. College of Chemistry and Chemical Engineering, Ocean University of China, qingdao 266003, China Correspondence should be addressed to Jiao Kui 1. College of Chemistry and Molecular Engineering Qingdao University of Science and Technology 266042 Qingdao China 2. College of Chemistry and Chemical Engineering Ocean University of China 266003 Qingdao China

The electrooxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) is dependent on the pH value of Britton-Robinson (B-R) buffer solution. In this work, the electrooxidation behavior of TMB was investigated with a SnO2:F film optically transparent thin-layer spectroelectrochemical cell. TMB underwent one two-electron electrooxidation process in the pH range from 2.0 to < 4.0, and two successive one-electron electrooxidation processes in the pH range from 4.0 to < 7.0 in the B-R buffer solution. At pH 6.5, the electrooxidative product of TMB generated a subsequent chemical reaction to yield an azo compound. Several spectroelectrochemical techniques, such as thin-layer cyclic voltammetry, thin-layer cyclic voltabsorptometry, thin-layer potential-controlled electrolysis absorptometry, thin-layer single-potential-step chronoabsorptometry, thin-layer dou-ble-potential-step chronoabsorptometry, thin-layer single-potential-step open-circuit relaxation chronoabsorptometry, were applied to this investigation. The formal potential E0’ and the electron transfer number corresponding to the electrooxidation of TMB in B-R buffer solution, and the reaction rate constant of the subsequent chemical reaction were determined.

关键词： 3,3′,5,5′-tetramethylbenzidine tin oxide film optically transparent electrode thin-layer spectroelectro-chemistry electrooxidation