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Antiviral effect of polysaccharide from Spirulina platensis(PSP) on HSV-2 in vitro

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Journal of Microbiology and Immunology 2006年第2期4卷 146-152页

作者： HONG YU XIAO ZHU wen qing zhang RUI Lü Department of Microbiology College of Medicine Qingdao University Qingdao P. R. China

To explore the antiviral effect and mechanism of polysaccharide from Spirulina platensis （PSP） on herpes simplex virus type 2 （HSV-2）, a standard strain of HSV-2 （333 strain） was used to investigate the antiviral effect of PSP in vitro. PSP in various concentrations was applied to different stages of HSV-2 replication cycle. Finally, the virus infectivity （TCID50）, cytopathic effect （CPE）, and MTT staining method for viable cells （MTT assay） were used as markers to evaluate the effect of PSP on HSV-2. The quantity of HSV-DNA was detected by real-time fluorescence quantitative PCR （FQ-PCR）. The HSV-2 infected Vero cell tdtrastructures were observed by transmission electron microscopy （TEM）. The results showed that PSP had little cytotoxic effect on Vero cells, it could not directly inactivate HSV-2 infectivity. PSP not only interfered in adsorption of HSV-2 to Vero cells but also inhibited HSV-2 biosynthesis in the ceils. FQ-PCR results showed that the inhibitory rate on HSV- DNA also increased in a dose-dependent and time-dependent manner. TEM also confirmed that PSP exhibited pronounced inhibitory effect on HSV-2. In conclusion, the antiviral effect of PSP on HSV-2 may be attributed to the inhibition of virus adsorption, virus replication and synthesis in cells.

关键词： Polysaccharide from Spirulina platensis (PSP) HSV-2 FQ-PCR TEM

Application of data warehouse in power transformer diagnosis system

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通讯和计算机（中英文版） 2009年第11期6卷 17-20页

作者： ZHAO wen-qing zhang Yan-fang WANG Xiao-hui School of Computer Science and Technology North China Electric Power University Baoding 071003 China

Studies on alkaloids from the twigs and leaves of Alstonia mairei

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Studies on alkaloids from the twigs and leaves of Alstonia m...

Different Strategies for Preparation of Non-tagged rV270 Protein and Its Efficacy against Yersinia Pestis Challenge

中国化学会第十二届全国天然有机化学学术会议

作者： Min Song Xiao-Qi zhang wen-Cai Ye qing-wen zhang State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical Sciences Institute of Traditional Chinese Medicine and Natural Products Jinan University

The genus Alstonia(Apocynaceae),comprises more than 60 species and is widely distributed in the tropical regions and subtropical areas of Africa,Central America,Southeast Asia,Polynesia and *** plants of Apocynaceae family are good sources of monoterpenoid indole *** monoterpenoid indole alkaloid were found to possess various pharmacological activities such as antiamoebic,anticancer,anti-inflammatory,antibacterial and anti-asthmatic *** is endemic in Yunnan,Guizhou and Sichuan provinces of *** the present work,chromatographic separation of 95% ethanol extract of *** has yielded 9 *** structures were elucidated as N-oxo-6α-hydroxypseudokopsine(1),6α-hydroxypseudokopsine(2),19-epivindolinine(3),vindolinine N-oxide(4),N-oxo-14,15-didehydroaspidofractinine(5),kopsiloscine G(6),6β-hydroxykopsinine(7).N-methylburnamine(8),and rhazimol(9).Among them,1 and 5 are new alkaloids,and 3,4,6,7,8 are isolated from *** for the first time.

关键词： Alstonia mairei Monoterpenoid indole alkaloids Structural elucidation Apocynaceae

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Biomedical and Environmental Sciences 2010年第5期23卷 333-340页

作者： WANG WANG ZHI-ZHEN QI qing-wen zhang BEN-CHUAN WU ZI-wen ZHU YONG-HAI YANG BAI-ZHONG CUI RUI-XIA DAI YE-FENG QIU ZU-YUN WANG ZHAO-BIAO GUO TAO-XING SHI HU WANG RUI-FU YANG XIAO-YI WANG Laboratory of Analytical Microbiology State Key Laboratory of Pathogen and BiosecurityBeijing Institute of icrobiology and EpidemiologyBeijing 100071China qinghai Institute for Endemic Disease Prevention and Control of qinghai Province Xining811602China Beijing Institute of Bioengneering Beijing 100071China

Objective LcrV is an important component for the development of a subunit vaccine against *** reduce immunosuppressive activity of LcrV,a recombinant LcrV variant lacking amino acids 271 to 326 （rV270） was prepared by different methods in this *** A new strategy that produced non-tagged or authentic rV270 protein was designed by insertion of rV270-thrombin-hexahistidine fusion gene into the vector pET24a,or by insertion of hexahistidine-enterokinase-rV270 or hexahistitine-factor Xa-rV270 fusion gene into the vector *** Co2＋ affinity chromatography,a purification strategy was developed by cleavage of His tag on column,following Sephacryl S-200HR column filtration *** Removal of His tag by thrombin,enterokinase and factor Xa displayed a yield of 99.5%,32.4% and 15.3%,*** Sephacryl S-200HR column filtration chromatography,above 97% purity of rV270 protein was *** rV270 that was adsorbed to 25% （v/v） Al（OH）3 adjuvant in phosphate-buffered saline （PBS） induced very high titers of antibody to rV270 in BALB/c mice and protected them （100% survival） against subcutaneous challenge with 106 CFU of *** virulent strain *** The completely authentic rV270 protein can be prepared by using enterokinase or factor Xa,but they exhibited extremely low cleavage activity to the corresponding recognition *** cleavage is an efficient strategy to prepare non-tagged rV270 protein and can be easily operated in a large scale due to its relatively low cost and high cleavage *** recombinant rV270 can be used as a key component to develop a subunit vaccine of plague.

关键词： Yersinia pestis rV270 antigen Purification Protection Plague

Cablinosides A and B, two polyketide glycosides from the aerial part of Pogostemon cablin

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Cablinosides A and B, two polyketide glycosides from the aer...

Effects of the polysaccharides from Spirulina platensis on the activities of hepatitis B virus

中国化学会第十届全国天然有机化学学术会议

作者： Ping Li Zhi-Qi Yin qing-wen zhang Institute of Chinese Medical Sciences University of Macau State Key Laboratory of Quality Research in Chinese Medicine (University of Macau) Department of phytochemistry China Pharmaceutical University

＜正＞Pogostemon cablin（Blanco）Benth.（Lamiaceae）is a perennial plant imported from tropical Asia into South China during untold ***,the aerial part of ***,has been widely used as a traditional Chinese medicine in the treatment of vomit,diarrhea and *** search for bioactive constituents from the herbs in Lingnan region,chemical investigation was

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Journal of Microbiology and Immunology 2005年第3期3卷 211-218页

作者： wen qing zhang BING LIU HONG YU SHOU YI DING XIU PING YU Institute of Pathogenic Biology Medical College of Shandong University 44 Wenhua Xilu Jinan P.R. China Department of Microbiology Medical School of Qingdao University 38 Dengzhou Road Qingdao P.R. China

To investigate the effects of the polysaccharides from Spirulina platensis (PSP) on hepatitis B virus (HBV), the cytotoxic effect of PSP was assessed by choosing the maximal concentration of PSP without cytotoxic effect on HepG2 2.2.15 cell line for further experiments. Four concentrations (400-50 μg/ml) of PSP were adopted to treat the cells, and the supernatants or cells were collected after 24, 48, 72 and 144 h respectively. HBsAg and HBeAg in the supernatants of cell cultures were tested with ELISA and copies of HBV DNA in supernatants were measured by real-time fluorescence quantitative PCR (FQ-PCR). Meanwhile, DNA/RNA hybridization was performed to evaluate the expression of IFN-α receptor (IFN-αR) on the cells. The experimental results showed that the secretion of HBV antigens decreased under the influence of PSP in a dose and time-dependent manner. PSP in concentration of 400 μg/ml could significantly decrease the secretion of HBsAg in 24 h. Although no obvious effect was observed on the secretion of HBeAg at that time, the inhibitory effects were observed in a dose-dependent manner from 48 to 144 h. In addition, the copies of HBV DNA were declined under the influence of PSP in the same manner, moreover, the maximal suppressive effect of PSP in concentration of 400 μg/ml was as great as that of lamivudine. The expression of IFN-αR was much higher in PSP-treated cells than that of the un-treated cells also in dose and time-dependent manner. It is concluded that PSP in non-cytotoxic concentration not only significantly decreases the secretion of the HBV antigens and the replication of HBV DNA, but also increases the expression of IFN-αR on HepG2 2.2.15 cell line. The results of the present investigation strongly support the notion that PSP exerts the anti-HBV effect both through enhancing the anti-HBV immunity and acting on HBV directly.

关键词： Polysaccharides of Spirulina platensis (PSP) HepG2 2.2.15 cell line HBeAg HBsAg HBV-DNA IFN-αR

Degradation of retinoid X receptor α by TPA through proteasome pathway in gastric cancer cells

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World Journal of Gastroenterology 2003年第9期9卷 1915-1919页

作者： Xiao-Feng Ye Su Liu Qiao Wu Xiao-Feng Lin Bing zhang Jia-Fa Wu Ming-qing zhang wen-Jin Su Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering,School of Life Sciences,Xiamen University,Xiamen 361005,Fujian Province,China Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering School of Life Sciences Xiamen University Xiamen 361005 Fujian Province China.

AIM: To investigate and determine the mechanism and signal pathway of tetradecanoylphorbol-1, 3-acetate (TPA) in degradation of RXRα.METHODS: Gastric cancer cell line, BGC-823 was used in the experiments. The expression level of R XRα protein was detected by Western blot. Nuclear and cytoplasmic protein fractions were prepared through lysis of cell and *** and translocation of RXRα were observed under laser-scanning confocal microscope through labeling specific anti-RXRα antibody and corresponding immunofiuorescent antibody as secondary antibody. Different inhibitors were used as ***: In BGC-823 cells, RXRα was expressed in the nucleus. When cells were treated with TPA, expression of RXRα was repressed in a time-dependent and TPAconcentration-dependent manner. Meanwhile, translocation of RXR from the nucleus to the cytoplasm occurred, also in a time-dependent manner. When cells were pre-incubated with proteasome inhibitor MG132 for 3 hrs, followed by TPA for another 12 hrs, TPA-induced RXRα degradation was inhibited. Further observation of RXRα translocation in the presence of MG132 showed that MG-132 could block TPAinduced RXRα redistribution. Conversely, when RXRαtranslocation was inhibited by LMB, an inhibitor for blocking protein export from the nucleus, TPA could not repress expression of RXRα.CONCLUSION: TPA could induce the degradation of RXRα protein in BGC-823 cells, and this degradation is time-and TPA-concentration-dependent. Furthermore, the degradation of RXRα by TPA is via a proteasome pathway and associated with RXRα translocation from the nucleus to the cytoplasm.

关键词：胃癌类维生素A受体X TPA BGC-823细胞

A DIACETYLENE CONTAINING α-NITRONYL NITROXIDE——SYNTHESIS,CRYSTAL STRUCTURE AND MAGNETIC CHARACTERIZATION

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Chinese Chemical Letters 1993年第4期4卷 287-288页

作者： De qing zhang wen Sheng ZhOU~a Dao Ben ZHU Institute of Chemistry,Academia Sinica,Beijing 100080 a.Dept,of Physics,Peking University,Beijing 100871

The mono-α-nitronyl nitroxide substituted diacetylene was synthesized and its structure was established by EA,FAB-MS,IR,ESR and x-ray diffraction *** the Extracting Sample Magnetometer,its magnetic properties was inv... 详细信息

关键词： NITRONYL NITROXIDE SYNTHESIS,CRYSTAL STRUCTURE AND MAGNETIC CHARACTERIZATION A DIACETYLENE CONTAINING

Observation of mother-perpetrated infanticide in golden takins(Budorcas taxicolor bedfordi)

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Zoological Research 2020年第4期41卷 455-457页

作者： Hai-Tao Zhao wen-qing zhang Kang-Sheng Jia Jia-Xuan Li Xu-Xiang Bai Xiao-Wei Wang Song-Tao Guo Shu-Jun He Hong-Juan Sun Ying-Hu Lei Ru Liang Pan Bao-Guo Li Shaanxi Key Laboratory for Animal Conservation Shaanxi Institute of ZoologyXi'anShaanxi 710032 China College of Life Sciences Northwest UniversityXianShaanxi 710069China Shaanxi Broadcasting Corporation Xi'anShaanxi 710061China Research Center of Qinling Giant Panda Shaanxi Academy of ForestryXi'anShaanxi 710402China University of California Davis Davis 95616USA Internationa/Centre of Biodiversity and Primate ConservationCentre Dali UniversityDaliYunnan 671000China School of Human Sciences University of Western AustraliaPerthWA 6009Australia Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunmingYunnan 650223China

DEAR EDITOR,Infanticide by unrelated individuals is widely reported in the animal kingdom;however,little is known about cases perpetrated by a parent,particularly the *** article reports on three cases of mother-initiated infanticide in Qinling golden takins(Budorcas taxicolor bedfordi)from video and camera *** on previous reports in other animals,we propose that the infanticide events observed in golden takins were related to the parental manipulation mechanism-i.e.

关键词： EDIT mother individual