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Six-fold swelling expansion microscopy assisted cell painting for high-content phenotypic screening

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Optoelectronics Letters 2025年

作者： Junfang XIE Yipeng DONG Jin WANG Zhichao deng Jianguo tian qing YE Key Laboratory of Weak-Light Nonlinear Photonics， Ministry of Education， School of Physics and TEDA Applied Physics， Nankai University

Imaging-based phenotypic screening uses cellular phenotypes to describe the drug performance， which generally focuses on single cellular feature， lacking of a comprehensive characterization. Here， we propose a high content phenotypic screening method based on expansion microscopy (ExM) assisted Cell Painting， which enables multi-channel imaging with approximately 50 nm resolution. As a demonstration， we applied this method to a phenotypic screening involving five drugs. The morphological attributes of three subcellular structures were summarized to consist a “fingerprint” describing the drug effect. The proposed method can provide comprehensive and detailed clues for drug evaluation， enriching the content of phenotypic screening.

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The composition of magmatic-hydrothermal fluids and their related metal mineralization

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Science China Earth Sciences 2025年第1期68卷 208-225页

作者： Qihai SHU Jun deng State Key Laboratory of Geological Processes and Mineral Resources School of Earth Sciences and Resources China University of Geosciences Frontiers Science Center for Deep-time Digital Earth China University of Geosciences

Magmatic hydrothermal deposits are important sources of many bulk, precious, and rare metals, and have attracted interest from both academic and industrial communities. However, it is still unclear if the major enrichment of the ore-forming metals occurs in the parental magma source region, during the magma evolution, or after the fluid exsolution. Fluid inclusions trapped in hydrothermal minerals crystallized in the early, high-temperature stage before metal precipitation record information close to that of the initially exsolved magmatic fluids, which are helpful in revealing whether the metals have been already significantly enriched prior to the hydrothermal stage. In this study, available compositional data of such early-stage fluid inclusions from magmatic hydrothermal systems globally have been compiled. The results indicate that the concentrations of Cu,Sn, and W respectively from the Cu-, Sn-, and W-mineralized fluids are significantly higher than the hydrothermal systems that do not produce ores with the corresponding metals. This suggests that Cu, Sn, and W could have already been enriched in the magma source and/or magma evolution stage. The Mo concentrations show no difference between Mo-mineralized and Mobarren fluid systems, indicating no significant pre-enrichment in the source region or during the magma evolution. This also implies that an efficient collection of Mo from a large volume of magmas is required to account for the formation of a large Mo deposit. Almost all the hydrothermal fluids contain high concentrations of Fe and Zn-Pb, but only a few of them eventually form Fe or Zn-Pb deposits, reflecting an important role of the hydrothermal evolution process on the selective metal *** above results indicate that different metals are enriched in different stages of a magmatic hydrothermal system, and that the major roles played in the formation of a deposit vary with the metal types. In addition to the metal concentrations in the o

关键词： Magmatic hydrothermal deposits Fluid inclusions LA-ICP-MS Metal enrichment Mineralization mechanism

A Cross-Multi-Domain Trust Assessment Authority Delegation Method Based on Automotive Industry Chain

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Computers, Materials & Continua 2025年第1期82卷 407-426页

作者： Binyong Li Liangming deng Jie Zhang Xianhui deng School of Cybersecurity(Xin Gu Industrial College) Chengdu University of Information TechnologyChengdu610225China Advanced Cryptography and System Security Key Laboratory of Sichuan Province Chengdu610225China SUGON Industrial Control and Security Center Chengdu610225China

To solve the challenges of connecting and coordinating multiple platforms in the automotive industry and to enhance collaboration among different participants,this research focuses on addressing the complex supply relationships in the automotive market,improving data sharing and interactions across various platforms,and achieving more detailed integration of data and *** propose a trust evaluation permission delegation method based on the automotive industry *** proposed method combines smart contracts with trust evaluation mechanisms,dynamically calculating the trust value of users based on the historical behavior of the delegated entity,network environment,and other factors to avoid malicious node attacks during the permission delegation *** also introduce strict control over the cross-domain permission granting and revocation mechanisms to manage the delegation path,prevent information leakage caused by malicious node interception,and effectively protect data integrity and *** analysis shows that this method meets the realtime requirements of collaborative interaction in the automotive industry chain and provides a feasible solution to permission delegation issues in the automotive industry chain,offering dynamic flexibility in authorization and scalability compared to most existing solutions.

关键词： Automotive industry chain cross-domain access block chain delegate authority trust assessment

Exploring the interaction between the gut microbiota and cyclic adenosine monophosphate-protein kinase A signaling pathway:a potential therapeutic approach for neurodegenerative diseases

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Neural Regeneration Research 2025年第11期20卷 3095-3112页

作者： Fengcheng deng Dan Yang Lingxi qing Yifei Chen Jilian Zou Meiling Jia Qian Wang Runda Jiang Lihua Huang College of Pharmacy Chengdu University of Traditional Chinese MedicineChengduSichuan ProvinceChina State Key Laboratory of Southwestern Chinese Medicine Resources Chengdu University of Traditional Chinese MedicineChengduSichuan ProvinceChina

The interaction between the gut microbiota and cyclic adenosine monophosphate(cAMP)-protein kinase A(PKA)signaling pathway in the host's central nervous system plays a crucial role in neurological diseases and enhances communication along the gut–brain *** gut microbiota influences the cAMP-PKA signaling pathway through its metabolites,which activates the vagus nerve and modulates the immune and neuroendocrine ***,alterations in the cAMP-PKA signaling pathway can affect the composition of the gut microbiota,creating a dynamic network of microbial-host *** reciprocal regulation affects neurodevelopment,neurotransmitter control,and behavioral traits,thus playing a role in the modulation of neurological *** coordinated activity of the gut microbiota and the cAMP-PKA signaling pathway regulates processes such as amyloid-β protein aggregation,mitochondrial dysfunction,abnormal energy metabolism,microglial activation,oxidative stress,and neurotransmitter release,which collectively influence the onset and progression of neurological *** study explores the complex interplay between the gut microbiota and cAMP-PKA signaling pathway,along with its implications for potential therapeutic interventions in neurological *** pharmacological research has shown that restoring the balance between gut flora and cAMP-PKA signaling pathway may improve outcomes in neurodegenerative diseases and emotional *** can be achieved through various methods such as dietary modifications,probiotic supplements,Chinese herbal extracts,combinations of Chinese herbs,and innovative dosage *** findings suggest that regulating the gut microbiota and cAMP-PKA signaling pathway may provide valuable evidence for developing novel therapeutic approaches for neurodegenerative diseases.

关键词： cyclic adenosine monophosphate emotional disorders gut microbiota neurodegenerative diseases neurological diseases protein kinase A reciprocal regulation signaling pathway strategy therapies

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Autophagy-targeting modulation to promote peripheral nerve regeneration

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Neural Regeneration Research 2025年第7期20卷 1864-1882页

作者： Yan Chen Hongxia deng Nannan Zhang Department of Obstetrics and Gynecology West China Second HospitalSichuan UniversityChengduSichuan ProvinceChina Key Laboratory of Birth Defects and Women and Children’s Diseases Ministry of EducationSichuan UniversityChengduSichuan ProvinceChina Laboratory of Reproductive Endocrinology and Reproductive Regulation Sichuan UniversityChengduSichuan ProvinceChina National Center for Birth Defect Monitoring West China Second University HospitalSichuan UniversityChengduSichuan ProvinceChina

Nerve regeneration following traumatic peripheral nerve injuries and neuropathies is a complex process modulated by diverse factors and intricate molecular *** studies have focused on factors that stimulate axonal outgrowth and myelin ***,recent studies have highlighted the pivotal role of autophagy in peripheral nerve regeneration,particularly in the context of traumatic ***,autophagy-targeting modulation has emerged as a promising therapeutic approach to enhancing peripheral nerve *** current understanding suggests that activating autophagy facilitates the rapid clearance of damaged axons and myelin sheaths,thereby enhancing neuronal survival and mitigating injury-induced oxidative stress and *** actions collectively contribute to creating a favorable microenvironment for structural and functional nerve regeneration.A range of autophagyinducing drugs and interventions have demonstrated beneficial effects in alleviating peripheral neuropathy and promoting nerve regeneration in preclinical models of traumatic peripheral nerve *** review delves into the regulation of autophagy in cell types involved in peripheral nerve regeneration,summarizing the potential drugs and interventions that can be harnessed to promote this *** hope that our review will offer novel insights and perspectives on the exploitation of autophagy pathways in the treatment of peripheral nerve injuries and neuropathies.

关键词： autophagy autophagy related genes Charcot–Marie–Tooth diseases diabetic peripheral neuropathy metformin myelination peripheral nerve injury Schwann cells sciatic nerve Wallerian degeneration

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A versatile synthetic strategy towards rare earth based metal-organic frameworks

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Science China Chemistry 2025年

作者： Quan Shi Yun-Hao Xu Ming-Hui Chen Peng Zhao Wen-Bo deng Ji Xiong Kai Guo Ya-qing Feng Bao Zhang School of Chemical Engineering and Technology Tianjin University tianjin Collaborative Innovation Center of Chemical Science and Engineering Guangdong Laboratory Chemistry & Fine Chemical Industry Jieyang Center

Rare earth metal-organic frameworks (RE-MOFs) have drawn wide attention owing to diverse network structures and various application ***,due to the unpredictable formation of the nodes,it is still challenging to design novel RE-MOFs.A novel versatile strategy was developed employing a RE-acetate complex (RE2(CH3COO)6·4H2O) as a precursor to construct RE-MOFs.2D RE-MOFs (RE-DBP) and 3D RE-MOFs (RE-TCPP) could be obtained by using dicarboxylic porphyrin ligands H2DBP and tetracarboxylic porphyrin ligands H2TCPP,*** RE-MOFs were further synthesized to verify the universality of the *** crystalline RE-MOFs could also be prepared at room temperature via the developed strategy,which could significantly promote large-scale preparation and practical application of *** proof of the concept,the performance of porphyrin-based RE-MOFs was investigated in the photocatalytic cycloaddition of CO2with *** the RE-DBP(Co) MOFs,Tb-DBP(Co) exhibited the best photocatalytic performance,which was also greater than that of the corresponding 3D MOF Tb-TCPP(Co) due to the optimal 2D structure and thus the accelerated photogenerated charge separation and transfer *** work not only provides a novel versatile strategy to design and develop RE-MOFs,but also proposes a promising approach for the potential bulk production of RE-MOFs at room temperature.

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COMPrompter: reconceptualized segment anything model with multiprompt network for camouflaged object detection

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Science China(Information Sciences) 2025年第1期68卷 189-203页

作者： Xiaoqin ZHANG Zhenni YU Li ZHAO deng-Ping FAN Guobao XIAO Zhejiang Province Key Laboratory of Intelligent Informatics for Safety and Emergency Wenzhou University Nankai International Advanced Research Institute (SHENZHEN FUtian) College of Computer Science Nankai University School of Computer Science and Technology Tongji University

We rethink the segment anything model(SAM) and propose a novel multiprompt network called COMPrompter for camouflaged object detection(COD). SAM has zero-shot generalization ability beyond other models and can provide an ideal framework for COD. Our network aims to enhance the single prompt strategy in SAM to a multiprompt strategy. To achieve this, we propose an edge gradient extraction module, which generates a mask containing gradient information regarding the boundaries of camouflaged objects. This gradient mask is then used as a novel boundary prompt, enhancing the segmentation process. Thereafter, we design a box-boundary mutual guidance module, which fosters more precise and comprehensive feature extraction via mutual guidance between a boundary prompt and a box prompt. This collaboration enhances the model's ability to accurately detect camouflaged objects. Moreover, we employ the discrete wavelet transform to extract high-frequency features from image embeddings. The high-frequency features serve as a supplementary component to the multiprompt ***, our COMPrompter guides the network to achieve enhanced segmentation results, thereby advancing the development of SAM in terms of COD. Experimental results across COD benchmarks demonstrate that COMPrompter achieves a cutting-edge performance, surpassing the current leading model by an average positive metric of 2.2% in COD10K. In the specific application of COD, the experimental results in polyp segmentation show that our model is superior to top-tier methods as well. The code will be made available at https://***/guobaoxiao/COMPrompter.

关键词： segment anything model camouflaged object detection boundary prompt

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Experimental Study on Diesel Spray and Flame Asymmetry Characteristics of Dual-Orifice Nozzles for a Medium-Speed Engine

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Journal of Thermal Science 2025年第1期34卷 303-322页

作者： JU Dehao LIU Rui ZHENG Liang deng Jiahui ZHANG Wenzheng HUO Jinlu HUANG Li Shanghai Marine Diesel Engine Research Institute National Key Laboratory of Marine Engine Science and Technology Key Laboratory for Power Machinery and Engineering Ministry of EducationShanghai Jiao Tong University

The asymmetry of the multi-orifice spray will cause uneven heat load of the marine diesel engine,thereby affecting its working performance and service ***,an in-depth understanding of the spray and flame characteristics of multi-orifice nozzles will guide the optimization of the nozzle structure,needle design and diesel atomization and combustion *** this reason,four groups of dual-orifice nozzles with different hole diameters(0.1-0.55 mm) and mass flow rates covering the typical marine medium-speed diesel injections are designed and customized,and the constant volume chamber(CVC) with high temperature and pressure is used to simulate the actual in-cylinder working conditions of the diesel engine for the spray visualization *** study the asymmetry of the fuel sprays discharged from a diesel injector,the multi-orifice nozzle is simplified as a dual-orifice nozzle in this *** with X-ray Computed Tomography(CT) imaging technology,the influences of the nozzle internal structure on the spray and flame asymmetry are studied in the constructed supercritical *** is found that the asymmetry of the inlet angle and the equivalent length-diameter ratio is positively correlated with the inconsistency of the dual *** an increase in the injection pressure and nozzle diameter,the asymmetry of the dual spray becomes more pronounced,resulting in greater disparities in the ignition delay times and ignition positions of the two ***,the increase in nozzle diameter also leads to combustion instability,resulting in a flame with a serrated *** the increase of ambient temperature,the proportion of liquid phase in the jet decreases and the relative density of spray front decreases.

关键词： atomization dual-orifice nozzle nozzle structure spray flame asymmetry

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平晶谱仪谱线波长的直接标定

中国激光 2003年第2期30卷 167-170页

作者：刘智李儒新贾天卿邓蕴沛范品忠曾志男徐至展中国科学院上海光学精密机械研究所强光光学开放实验室上海201800

提出一种简便的新方法用以实现X射线平晶谱仪谱线波长的直接标定 ,只需在谱仪晶体表面加上一个特制的辅助光阑 ,即可在不知光源位置和没有任何参考谱线的情况下精确标定谱线的波长。

关键词：谱线波长直接标定平晶谱仪 X射线光谱学激光等离子体

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