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Effects of long non-coding RNA myocardial infarction-associated transcript on retinal neovascularization in a newborn mouse model of oxygen-induced retinopathy

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Neural Regeneration Research 2021年第9期16卷 1877-1881页

作者： Yu Di Yue Wang Xue Wang qing-zhu nie Department of Ophthalmology Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina

Whether long non-coding RNA myocardial infarction-associated transcript is involved in oxygen-induced retinopathy remains poorly understood. To validate this hypothesis, we established a newborn mouse model of oxygen-induced retinopathy by feeding in an oxygen concentration of 75 ± 2% from postnatal day 8 to postnatal day 12, followed by in normal air. On postnatal day 11, the mice were injected with the myocardial infarction-associated transcript siRNA plasmid via the vitreous cavity to knockdown long non-coding RNA myocardial infarction-associated transcript. Myocardial infarction-associated transcript siRNA transcription significantly inhibited myocardial infarctionassociated transcript mRNA expression, reduced the phosphatidylinosital-3-kinase, phosphorylated Akt and vascular endothelial growth factor immunopositivities, protein and mRNA expression, and alleviated the pathological damage to the retina of oxygen-induced retinopathy mouse models. These findings suggest that myocardial infarction-associated transcript is likely involved in the retinal neovascularization in retinopathy of prematurity and that inhibition of myocardial infarction-associated transcript can downregulate phosphatidylinosital-3-kinase, phosphorylated Akt and vascular endothelial growth factor expression levels and inhibit neovascularization. This study was approved by the Animal Ethics Committee of Shengjing Hospital of China Medical University, China(approval No. 2016 PS074 K) on February 25, 2016.

关键词： long non-coding RNA myocardial infarction-associated transcript neovascularization neurovascular prematurity retinopathy vascular development vascular endothelial growth factor

Matrix metalloproteinase-9 and vascular endothelial growth factor expression change in experimental retinal neovascularization

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International Journal of Ophthalmology(English edition) 2016年第6期9卷 804-808页

作者： Yu Di qing-zhu nie Xiao-Long Chen Department of Ophthalmology Shengjing Hospital of China Medical University

AIM： To investigate the signal transduction mechanism of matrix metalloproteinase-9 （MMP-9） mediatedvascular endothelial growth factor （VEGF） expression and retinal neovascularization （RNV） in oxygen-induced retinopathy （OIR） model. METHODS： C57BL/6J mice were divided into four groups： control group, OIR group, OIR control group （phosphatebuffered saline by intravitreal injection） and treated group [tissue inhibitor of matrix metalloproteinase-1 （TIMP-1） by intravitreal injection]. OIR model was established in C57BIJ6J mice exposed to 75% ＋2% oxygen for 5d. mRNA level and protein expression of MMP-9, TIMP-1 and VEGF were measured by real-time polymerase chain reaction and Western blotting, and located by immunohistochemistry. RESULTS： Levels of MMP-9 and VEGF in retina were significantly increased in animals with OIR and OIR control group. Levels of TIMP -1 in retina was significantly reduced in animals with OIR and OIR control group. Furthermore, a significant correlation was found between MMP-9 and VEGF. Intravitreal injection of TIMP- 1 significantly reduced MMP-9 and VEGF expression of the OIR mouse model （all P〈0.05）. CONCLUSION： These results demonstrate that MMP- 9-mediated up-regulation of VEGF promotes RNV in retinopathy of prematurity （ROP）. TIMP-1 may be a potential target for the prevention and treatment of ROP.

关键词： retinal neovascularization matrixmetalloproteinase-9 vascular endothelial growth factor oxygen-induced retinopathy

Mechanism of piR-1245/PIWI-like protein-2 regulating Janus kinase-2/signal transducer and activator of transcription-3/vascular endothelial growth factor signaling pathway in retinal neovascularization

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Neural Regeneration Research 2023年第5期18卷 1132-1138页

作者： Yong Yu Li-Kun Xia Yu Di qing-zhu nie Xiao-Long Chen Department of Ophthalmology Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina

Inhibiting retinal neovascularization is the optimal strategy for the treatment of retina-related diseases, but there is currently no effective treatment for retinal neovascularization. P-element-induced wimpy testis(PIWI)-interacting RNA(piRNA) is a type of small non-coding RNA implicated in a variety of diseases. In this study, we found that the expression of piR-1245 and the interacting protein PIWIL2 were remarkably increased in human retinal endothelial cells cultured in a hypoxic environment, and cell apoptosis, migration, tube formation and proliferation were remarkably enhanced in these cells. Knocking down piR-1245 inhibited the above phenomena. After intervention by a p-JAK2 activator, piR-1245 decreased the expression of hypoxia inducible factor-1α and vascular endothelial growth factor through the JAK2/STAT3 pathway. For in vivo analysis, 7-day-old newborn mice were raised in 75 ± 2% hyperoxia for 5 days and then piR-1245 in the retina was knocked down. In these mice, the number of newly formed vessels in the retina was decreased, the expressions of inflammationrelated proteins were reduced, the number of apoptotic cells in the retina was decreased, the JAK2/STAT3 pathway was inhibited, and the expressions of hypoxia inducible factor-1α and vascular endothelial growth factor were decreased. Injection of the JAK2 inhibitor JAK2/TYK2-IN-1 into the vitreous cavity inhibited retinal neovascularization in mice and reduced expression of hypoxia inducible factor-1α and vascular endothelial growth factor. These findings suggest that piR-1245 activates the JAK2/STAT3 pathway, regulates the expression of hypoxia inducible factor-1α and vascular endothelial growth factor, and promotes retinal neovascularization. Therefore, piR-1245 may be a new therapeutic target for retinal neovascularization.

关键词： angiogenesis human retinal endothelial cells hypoxia inducible factor-1α hypoxia interleukin-1β migration non-coding RNA oxygen-induced injury PIWI-interacting RNA retinopathy

Clinical effect of improved viscocanalostomy for the treatment of primary congenital glaucoma

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International Journal of Ophthalmology(English edition) 2012年第4期5卷 466-468页

作者： Yong Yu Zhi-Li Liu Lei Cao and qing-zhu nie Department of Ophthalmology Shengjing Hospital China Medical University Shenyang 110004 Liaoning Province China

AIM: To evaluate the clinical effect of improved viscocanalostomy in patients with primary congenital glaucoma. METHODS: Retrospective analysis of improved viscocanalostomy was performed on 51 eyes of 42 patients with primary congenital glaucoma. The outcome evaluation included postoperative intraocular pressure (IOP), corneal diameter, cup/disc ratio and complications. All patients were followed up at week 1, month 1, 3, 6 and 12. RESULTS: The results revealed that postoperative IOP was decreased from (38.57 +/- 13.61)mmHg to (10.53 +/- 3.91)mmHg. (14.89 +/- 5.26)mmHg,(15.42 +/- 5.11)mmHg, (13.82 +/- 3.46)mmHg, (13.16 +/- 5.29)mmHg at follow-up time of 1 week,1, 3, 6, 12 months (P0.001). The postoperative corneal diameter was decreased significantly (P=0.002); The mean cup/dish ratio wasn't significantly different (P=0.148) before and after the surgery, the cup/dish ratio of successful surgery was evidently decreased (preoperative 0.7 +/- 0.2, postoperative 0.6 +/- 0.3, P = 0.007), but the complications like unformed anterior chamber were not observed. The mean follow-up period was 12 months. CONCUJSION: Improved viscocanalostomy improves the clinical effects of the patients with primary congenital glaucoma, such as higher success rates, lower postoperative mean IOP and fewer complications.

关键词： primary congenital glaucoma improved viscocanalostomy intraocular pressure

Maternally expressed gene 3 regulates retinal neovascularization in retinopathy of prematurity

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Neural Regeneration Research 2022年第6期17卷 1364-1368页

作者： Yu Di Yue Wang Yue-Xia Wang Xue Wang Yuan Ma qing-zhu nie Department of Ophthalmology Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina

The mouse model of oxygen induced retinopathy is suitable for the study of various retinal neovascularization diseases,including retinopathy of *** maternally expressed gene 3(MEG3)has been demonstrated to have an inhibitory effect on diabetic *** this study,we investigated the role of MEG3 overexpression in oxygen-induced retinopathy in *** results showed that MEG3 overexpression effectively inhibited the production of retinal neovascularization in oxygen-induced retinopathy *** acts by down-regulating the expression of phosphoinositide 3-kinase,serine/threonine kinase,and vascular endothelial growth factor and pro-inflammatory ***3 overexpression lentivirus has a future as a new method for the clinical treatment of retinopathy of *** animal experiments were approved by the Animal Ethics Committee of Shengjing Hospital of China Medical University,China(approval No.2016PS074K)on February 25,2016.

关键词： long noncoding RNA maternally expressed gene 3 neurodevelopment oxygen-induced retinopathy phos

Mn/S diatomic sites in C3N4 to enhance O2 activation for photocatalytic elimination of emerging pollutants

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Journal of Environmental Sciences 2025年第3期149卷 512-523页

作者： Chengji Sui Zixuan nie Xiaobin Xie Yifeng Wang Lingshuai Kong Shou-qing Ni Jinhua Zhan Key Laboratory for Colloid&Interface Chemistry of Education Ministry School of Chemistry and Chemical EngineeringShandong UniversityJinan 250100China Institute of Eco-Environmental Forensics School of Environmental Science and EngineeringShandong UniversityQingdao 266237China

Oxygen activation leading to the generation of reactive oxygen species(ROS)is essential for photocatalytic environmental *** limited efficiency of O_(2)adsorption and reductive activation significantly limits the production of ROS when employing C_(3)N_(4)for the degradation of emerging *** with metal single atoms may lead to unsatisfactory efficiency,due to the recombination of photogenerated electron-hole ***,Mn and S single atoms were introduced into C_(3)N_(4),resulting in the excellent photocatalytic ***/S-C_(3)N_(4)achieved 100%removal of bisphenol A,with a rate constant 11 times that of pristine C_(3)N_(4).According to the experimental results and theoretical simulations,Satoms restrict holes,facilitating the photo-generated carriers’***-atom Mn acts as the O_(2)adsorption site,enhancing the adsorption and activation of O_(2),resulting the generation of *** study presents a novel approach for developing highly effective photocatalysts that follows a new mechanism to eliminate organic pollutants from water.

关键词： Photocatalytic degradation O_(2)activation Reactive oxygen species Emerging pollutants Single-atom catalyst

Effect of pyrite content on chalcopyrite flotation under different regrinding conditions

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 49-57页

作者： Zejun Wang qing Shi Guofan Zhang Yuxuan zhu Binbin Li School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083China Key Laboratory of Hunan Province for Clean and Efficient Utilization of Strategic Calcium-containing Mineral Resources Central South UniversityChangsha 410083China

This study aimed to investigate the effect of varying pyrite(Py)content on copper(Cu)in the presence of different regrinding conditions,which were altered using different types of grinding media:iron,ceramic balls,and their mixture,followed by flotation in the cleaner *** flotation performance of rough Cu concentrate can be improved by changing the regrinding conditions based on the Py *** electron microscopy,X-ray spectrometry,ethylenediaminetetraacetic acid disodium salt extraction,and X-ray photoelectron spectroscopy studies illustrated that when the Py content was high,the use of iron media in regrinding promoted the generation of hydrophilic Fe OOH on the surface of Py and improved the Cu *** ceramic medium with a low Py content prevented excessive Fe OOH from covering the surface of chalcopyrite(Cpy).Electrochemical studies further showed that the galvanic corrosion current of Cpy-Py increased with the addition of Py and became stronger with the participation of iron media.

关键词： chalcopyrite regrinding media pyrite galvanic corrosion flotation

A Novel Vibration-Based Self-Adapting Method to Acquire Real-Time Following Distance for Virtually Coupled Trains

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IEEE/CAA Journal of Automatica Sinica 2025年第1期12卷 27-39页

作者： qinglai Zhang Jianmin Gao qing Wu qinglie He Libin Tie Wanming Zhai Shengyang zhu State Key Laboratory of Rail Transit Vehicle System Southwest Jiaotong University IEEE Centre for Railway Engineering Central Queensland University National Energy Railway Equipment Co. LTDNational Energy Group

Virtual coupling(VC) is an emerging technology for addressing the shortage of rail transportation capacity. As a crucial enabling technology, the VC-specific acquisition of train information, especially train following distance(TFD), is *** this paper, a novel method is proposed to acquire real-time TFD by analyzing the vibration response of the front and following trains, during which only onboard accelerometers and speedometers are required. In contrast to the traditional arts of train positioning, this method targets a relative position between two adjacent trains in VC operation, rather than the global positions of the trains. For this purpose, an adaptive system containing three strategies is designed to cope with possible adverse factors in train operation. A vehicle dynamics simulation of a heavy-haul railway is implemented for the evaluation of feasibility and performance. Furthermore, a validation is conducted using a set of data measured from in-service Chinese high-speed trains. The results indicate the method achieves satisfactory estimation accuracy using both simulated and actual data. It has favorable adaptability to various uncertainties possibly encountered in train operation. Additionally, the method is preliminarily proven to adapt to different locomotive types and even different rail transportation modes. In general, such a method with good performance, low-cost, and easy implementation is promising to apply.

关键词： Adaptive system train following distance (TFD) train positioning vehicle dynamics vibration response virtual coupling (VC)

Utilizing electronic assisted enhancement:An innovative approach for studying the thermal decomposition and combustion of ionic liquids

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Defence Technology(防务技术) 2025年第2期44卷 179-189页

作者： Cailing Zhang Yutao Wang Baiquan Chen Zhenguo Pang Hongqi nie Quan zhu Peijin Liu Wei He College of Chemical Engineering Sichuan UniversityChengdu 610065China School of Aeronautics and Astronautics Sichuan UniversityChengdu 610065China School of Environmental and Safety Engineering North University of ChinaTaiyuan 030051China Science and Technology on Combustion Internal Flow and Thermostructure LaboratoryNorthwestern Polytechnical UniversityXi'an 710072China

Flammable ionic liquids exhibit high conductivity and a broad electrochemical window,enabling the generation of combustible gases for combustion via electrochemical decomposition and thermal *** characteristic holds significant implications in the realm of novel satellite *** a fraction of the electrical energy into energetic ionic liquid fuels,the thermal decomposition process is facilitated by reducing the apparent activation energy required,and electrical energy can trigger the electrochemical decomposition of ionic liquids,presenting a promising approach to enhance combustion efficiency and energy *** study applied an external voltage during the thermal decomposition of 1-ethyl-3-methylimidazole nitrate([EMIm]NO_(3)),revealing the effective alteration of the activation energy of[EMIm]NO_(3).The pyrolysis,electrochemical decomposition,and electron assisted enhancement products were identified through Thermogravimetry-Differential scanning calorimetry-Fourier transform infrared-Mass spectrometry(TG-DSC-FTIR-MS)and gas chromatography(GC)analyses,elucidating the degradation mechanism of[EMIm]NO_(3).Furthermore,an external voltage was introduced during the combustion of[EMIm]NO_(3),demonstrating the impact of voltage on the combustion process.

关键词： Flammable ionic liquids Kinetic methods Electron assisted enhanced thermal decomposition Electron assisted enhanced combustion