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A novel hepatitis B virus mutant with A-to-G at nt551 in the surface antigen gene

World Journal of Gastroenterology 2003年第2期9卷 304-308页

作者： Hua-Biao Chen De-Xing Fang Fa-qing li hui-Ying Jing Wei-Guo Tan Su-Qin li Huadong Research Institute for Medical Biotechnics, Nanjing 210002, Jiangsu Province, China Huadong Research Institute for Medical Biotechnics Nanjing 210002 Jiangsu Province China. chenhuabiao@***

AIM: Hepatitis B surface antigen (HBsAg) mutant of hepatitisB virus (HBV) is one of the important factors that result inimmune escape and cause failure of immunization. In thisstudy we reported and characterized a novel HBV mutantwith A-to-G at nt551 and intended to provide theoreticaldata for prevention of HBV infection in ***: A methodology comprising polymerase chainreaction (PCR) amplifying, M13 bacteriophage cloning andnucleotide sequencing was used to analyze the sera of thepediatric patient who was hepatitis B (HB) immune *** plasmids containing the mutant S gene and awild-type (adr) S gene were constructed respectively andthe recombinant HBsAg were expressed in COS-7 cells underthe regulation of SV40 early promoter. The recombinantproteins were investigated for their immunological reactivitywith different monoclonal antibodies (mAb) against 'a'determinant and vaccine-raised human ***: It was found that there was a new point mutationat nt551 of the HBV (adr) genome from A to G, leading to asubstitution of methionine (Met) to valine (Val) at position133 in the 'a' determinant of HBsAg. Compared to the wild-type HBsAg, the binding activity of the muant HBsAg tomAbs (A6, A11 and S17) and to vaccine-raised human anti-hepatitis B surface antibody (anti-HBs) decreased ***: According to the facts that the patient hasbeen immunized with HB vaccine and that the serum is anti-HBs positive and HBsAg negative, and based on thenucleotide sequence analysis of the mutant HBV S geneand its alteration of antigenicity, the HBV is considered tobe a new vaccine-induced immune escape mutant differentfrom the known ones.

关键词：乙型肝炎病毒表面抗原免疫逃逸免疫疗法抗体

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Accumulated Clinical Experiences from Successful Treatment of 1377 Severe and Critically Ill COVID-19 Cases

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Current Medical Science 2020年第4期40卷 597-601页

作者： Yong GAO Hai-bo QIU Shu ZHOU Zhen-ning WANG Jian-chu ZHANG Zheng-liang ZHANG Zhao-xin QIAN Hong-bo WANG Shi-huan YU Yi-feng LUO Ya dan WANG Zhuang liU Wei-ci WANG Ming JIA li-ming ZHANG Yu ZHANG Yang JIN Yong ZHANG Yong liU Zi-hua ZHOU Ying SU hui-qing li Wei-min XIAO Kai HUANG Ping HE Gang li Zhao-hui FU Shi liU Neng xing liN Fan-jun CHENG Union Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Zhongda Hospital of Souheast University Nanjing 210009China The First Affiliated Hospital of China Medical University Shenyang 110001China The Second Affiliated Hospital of Xi'an Jiaotong University Xi'an 710004China Xiangya Hospital of Zhongnan University Changsha 410008China The First Afiliated Hospital of Harbin Medical University Harbin 150001China The First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080China Beijing Friendship Hospital of Capital Medical University Beijing 100050China Beijing Anzhen Hospital of Capital Medical University Beijing 100029China Beijing Chaoyang Hospital of Capital Medical University Beiing 100020China

Summary:In late December 2019,COVID-19 was firstly recognized in Wuhan,China and spread rapidly to all of the provinces of *** West Campus of Wuhan Union Hospital,the designated hospital to admit and treat the severe and critically ill COVID-19 cases,has treated a large number of such patients with great success and obtained lots of valuable experiences based on the Chinese guideline(V7.0).To standardize and share the treatment procedures of severe and critically ill cases,Wuhan Union Hospital has established a working group and formulated an operational recommendation,including the monitoring,early warning indicators,and several treatment principles for severe and critically ill *** treatment experiences may provide some constructive suggestions for treating the severe and critically ill COVID-19 cases all over the world.

关键词： 2019-nCoV COVID-19 pneumonia treatment guidelines

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多孔金属纤维烧结板多尺度形貌的快速三维建模

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软件学报 2016年第10期27卷 2622-2631页

作者：徐志佳王清辉李静蓉华南理工大学机械与汽车工程学院广东广州510640

针对多孔金属纤维烧结板这一新型功能材料的多尺度形貌建模问题,基于机械加工表面微观形貌中存在的自仿射分形特性,拓展前期发展的三周期极小化曲面与Weierstrass-Mandelbrot分形几何快速合成方法,重点根据多孔金属纤维烧结板的结构特点... 详细信息

针对多孔金属纤维烧结板这一新型功能材料的多尺度形貌建模问题,基于机械加工表面微观形貌中存在的自仿射分形特性,拓展前期发展的三周期极小化曲面与Weierstrass-Mandelbrot分形几何快速合成方法,重点根据多孔金属纤维烧结板的结构特点,对Marching Cubes算法进行优化,以更高效地建立烧结板三维多尺度形貌的几何模型.同时,引入参数表征方法驱动分形TPMS对烧结板的形貌进行调整与控制.实例分析结果表明,所提出的方法可更有效地建立/控制多孔金属纤维烧结板的多尺度形貌模型;模型的有效性通过数值仿真和与其他方法的比较得到了验证.该建模方法可直接用于其他具有多尺度形貌的功能材料的数字化设计中,并促进其数值模拟技术的发展.

关键词：多孔金属纤维烧结板多尺度形貌周期曲面分形几何三维建模功能材料

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Hypidone hydrochloride(YL-0919)ameliorates functional deficits after traumatic brain injury in mice by activating the sigma-1 receptor for antioxidation

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Neural Regeneration Research 2025年第8期20卷 2325-2336页

作者： Yafan Bai hui Ma Yue Zhang Jinfeng li Xiaojuan Hou Yixin Yang Guyan Wang Yunfeng li Department of Anesthesiology Beijing Tongren HospitalCapital Medical UniversityBeijingChina Beijing Institute of Basic Medical Sciences BeijingChina Hebei North University ZhangjiakouHebei ProvinceChina Beijing Institute of Pharmacology and Toxicology State Key Laboratory of Toxicology and Medical CountermeasuresBeijing Key Laboratory of NeuropsychopharmacologyBeijingChina

Traumatic brain injury involves complex pathophysiological mechanisms,among which oxidative stress significantly contributes to the occurrence of secondary *** this study,we evaluated hypidone hydrochloride(YL-0919),a self-developed antidepressant with selective sigma-1 receptor agonist properties,and its associated mechanisms and targets in traumatic brain *** experiments to assess functional deficits were followed by assessment of neuronal damage through histological analyses and examination of blood-brain barrier permeability and brain ***,we investigated the antioxidative effects of YL-0919 by assessing the levels of traditional markers of oxidative stress in vivo in mice and in vitro in HT22 ***,the targeted action of YL-0919 was verified by employing a sigma-1 receptor antagonist(BD-1047).Our findings demonstrated that YL-0919 markedly improved deficits in motor function and spatial cognition on day 3 post traumatic brain injury,while also decreasing neuronal mortality and reversing blood-brain barrier disruption and brain ***,YL-0919 effectively combated oxidative stress both in vivo and in *** protective effects of YL-0919 were partially inhibited by *** results indicated that YL-0919 relieved impairments in motor and spatial cognition by restraining oxidative stress,a neuroprotective effect that was partially reversed by the sigma-1 receptor antagonist ***-0919 may have potential as a new treatment for traumatic brain injury.

关键词： antidepressant drug blood-brain barrier cognitive function hypidone hydrochloride(YL-0919) neurological function nuclear factor-erythroid 2 related factor 2 oxidative stress sigma-1 receptor superoxide dismutase traumatic brain injury

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A mutation specific polymerase chain reaction for detecting hepatitis B virus genome mutations at nt551

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World Journal of Gastroenterology 2003年第3期9卷 509-512页

作者： Chun-ling Ma De-Xing Fang Hua-Biao Chen Fa-qing li hui-Ying Jin Su-Qin li Wei-Guo Tan Huadong Research Institute for Medical Biotcchnics, Nanjing 210002, Jiangsu Province, China Huadong Research Institute for Medical Biotechnics Nanjing 210002 China. mchunling@***

AIM: The hepatitis B surface antigen (HBsAg) is considered to be one of the best markers for the diagnosis of acute and chronic HBV infection. But in some patients, this antigen cannot be detected by routine serological assays despite the presence of virus. One of the most important explanations for the lack of detectable HBsAg is that mutations which occur within the 'a' determinant of HBV S gene can alter expression of HBsAg and lead to changes of antigenicity and immunogenicity of HBsAg accordingly. As a result, these mutants cannot be detected by diagnosis assays. Thus, it is essential to find out specific and sensitive methods to test the new mutants and further investigate their distribution. This study is to establish a method to investigate the distribution of the HBsAg mutant at ***: A mutation specific polymerase chain reaction (msPCR) was established for amplifying HBV DNA with a mutation at nt551. Four sets of primer pairs, P551A-PPS,P551G-PPS, P551C-PPS and P551T-PPS, with the same sequences except for one base at 3' terminus were designed and synthesized according to the known HBV genome sequences and the popular HBV subtypes, adr and adw, in China. At the basis of regular PCR method, we explored the specific conditions for amplifying HBV DNAs with a mutation at nt551 by regulating annealing temperature and the concentration of these primers. 126 serum samples from patients of hepatitis B were collected, among which 16 were positive for HBV S DNA in the nested PCR amplification. These 16 HBV S DNAs were detected by using the msPCR ***: When the annealing temperature was raised to 71 °C, nt551A and nt551G were amplified specifically by P551A-PPS and P551G-PPS; At 72 °C and 5 pmole of the primers (each) in reaction of 25 μl volume, nt551C and nt551T were amplified specifically by P551C-PPS and P551TPPS. 16 of HBV S gene fragments were characterized by using this method. 14 of them were positive for nt551A, one was positive for nt551G

关键词：乙型肝炎病毒基因突变聚合酶链反应乙型肝炎表面抗原血清学检查

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Immunosuppressive effects of dihydroetorphine in its dependent rodents

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Acta Pharmacologica Sinica 1999年第5期 90-91页

作者： WU Wei-Ran, ZHENG Ji-Wang, li Na, li Ying, ZHENG Ke-Ren, BAI hui-qing, li Feng-Yuan ( Department of Pharmacology, Xi-an Medical University, Xi-an 710061 2National Institute on Drug Dependence Department of Immunology, Beijing Medical University, Beijing 100083, China)

To study the immunomodulatory effects of dihydreetorphine (DHE), the most powerful nacrotic analgesic synthesized up to now, the present studies systematically investigated its actions on immune functions in sub-chronically administered mice,72 h computer-controlled automatic intravenous injection rats,and intravenous self-administration *** significantly lowered the increase of body weight, decreased spleen and thymus weights, weakened the delayed-type hypersensitivity, reduced the

关键词： Immunosuppressive effects of dihydroetorphine in its dependent rodents

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Vector meson's spin alignments in high energy reactions

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 5-20页

作者： Jin-hui Chen Zuo-Tang liang Yu-Gang Ma Xin-li Sheng Qun Wang Key Laboratory of Nuclear Physics and Ion-beam Application (MoE) Institute of Modern PhysicsFudan University Institute of Frontier and Interdisciplinary Science Key Laboratory of Particle Physics and Particle Irradiation (MOE)Shandong University INFN-Firenze Department of Modern Physics University of Science and Technology of China School of Mechanics and Physics Anhui University of Science and Technology

The global spin alignment of vector mesons has been observed by the STAR collaboration at the Relativistic Heavy Ion Collider （RHIC） at Brookhaven National Laboratory （BNL）.It provides a unique opportunity to probe the correlation between the polarized quark and antiquark in the strongly coupled quark-gluon plasma （sQGP） produced in relativistic heavy ion collisions,opening a new window to explore the properties of s *** addition,spin alignments of vector mesons have also been observed in other high-energy particle collisions such as e+e-annihilations at high energies where hadron production is dominated by quark fragmentation *** results obtained are quite different from those obtained in heavy ion collisions where quark coalescence/combination mechanism dominates suggesting strong dependence on hadronization *** comprehensive studies in different hadronization processes are *** this article,we present a brief review of theoretical and experimental advances in the study of vector meson’s spin alignments in a variety of high-energy particle collisions,with emphasis on hadronization mechanisms.

关键词： vector meson spin alignment global polarization relativistic heavy ion collisions fragmentation

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Intramuscular hepatitis B immune globulin combined with lamivudine in prevention of hepatitis B recurrence after liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2006年第3期5卷 360-363页

作者： Mao-lin Yan, Lu-Nan Yan, Bo li, Yong Zeng, Tian-Fu Wen, Wen-Tao Wang, Jia-Yin Yang, Ming-qing Xu, Zhi-hui li and Yong-Bing Chen liver Transplantation Group of Transplantation Center, West China Hospital, Sichuan University, Chengdu 610041, China liver Transplantation Group of Transplantation Center West China Hospital Sichuan University Chengdu 610041 China.

BACKGROUND: Combined hepatitis B immune globulin (HBIg) and lamivudine in prophylaxis of the recurrence of hepatitis B after liver transplantation has significantly improved the survival of HBsAg positive patients. This study was undertaken to evaluate the outcomes of liver transplantation for patients with hepatitis B virus (HBV). METHODS: A retrospective chart analysis and a review of the organ transplant database identified 51 patients (43 men and 8 women) transplanted for benign HBV-related cirrhotic diseases between June 2002 and December 2004 who had survived more than 3 months. HBIg was administered intravenously during the first week and intramuscularly thereafter. RESULTS: At a median follow-up of 14.1 months, the overall recurrence rate in the 51 patients was 3.9% (2/51). The overall patient survival was 88.3%, and 82.4% after 1 and 2 years, respectively. A daily oral dose of 100 mg lamivudine for 2 weeks before transplantation for 10 patients enabled 57.1% (4/7) and 62.5% (5/8) of HBV-DNA and HBeAg positive patients respectively to convert to be negative. Intramuscular HBIg was well tolerated in all patients. CONCLUSION: Lamivudine combined with intramuscular HBIg can effectively prevent allograft from the recurrence of HBV after liver transplantation.

关键词： lamivudine hepatitis B immune globulin hepatitis B virus recurrence liver transplantation

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饱和非线性介质中艾里-高斯光束的传输与交互作用

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物理学报 2016年第24期65卷 58-65页

作者：陈卫军卢克清惠娟利张宝菊天津工业大学光电检测技术与系统天津市重点实验室天津300387 天津工业大学电子与信息工程学院天津300387

研究了艾里-高斯光束在饱和非线性介质中的传输与交互作用.结果表明,入射光为单艾里-高斯光束时,在一定功率范围内,调节初始振幅与光场分布,可形成传输方向可控的呼吸孤子.当初始振幅增加时,孤子的呼吸周期先减小后增大.入射光为两艾里... 详细信息

研究了艾里-高斯光束在饱和非线性介质中的传输与交互作用.结果表明,入射光为单艾里-高斯光束时,在一定功率范围内,调节初始振幅与光场分布,可形成传输方向可控的呼吸孤子.当初始振幅增加时,孤子的呼吸周期先减小后增大.入射光为两艾里-高斯光束时,同相位光束相互吸引,两光束中心位置两侧附近产生呼吸孤子和对称孤子对.反相位光束相互排斥,中心位置两侧仅产生对称的孤子对.光场分布越趋于高斯分布,两艾里-高斯光束相互作用就越强,孤子对的数目越少.

关键词：非线性光学饱和介质艾里-高斯光束交互作用

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基于内容的图像分割方法综述

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软件学报 2017年第1期28卷 160-183页

作者：姜枫顾庆郝慧珍李娜郭延文陈道蓄计算机软件新技术国家重点实验室(南京大学) 江苏南京210023 南京大学计算机科学与技术系江苏南京210023 南京理工大学泰州科技学院移动互联网学院江苏泰州225300 南京工程学院通信工程系江苏南京211167

图像分割是指将图像分成若干具有相似性质的区域的过程,是许多图像处理任务的预处理步骤.近年来,国内外学者主要研究基于图像内容的分割算法.在广泛调研大量文献和最新成果的基础上,将图像分割算法分为基于图论的方法、基于像素聚类的... 详细信息

图像分割是指将图像分成若干具有相似性质的区域的过程,是许多图像处理任务的预处理步骤.近年来,国内外学者主要研究基于图像内容的分割算法.在广泛调研大量文献和最新成果的基础上,将图像分割算法分为基于图论的方法、基于像素聚类的方法和语义分割方法这3种类型并分别加以介绍.对每类方法所包含的典型算法,尤其是最近几年利用深度网络技术的语义图像分割方法的基本思想、优缺点进行了分析、对比和总结.介绍了图像分割常用的基准数据集和算法评价标准,并用实验对各种图像分割算法进行对比.最后进行总结,并对未来可能的发展趋势加以展望.

关键词：图像分割图论聚类语义分割深度神经网络

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