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Effects of curcumin nanoparticles on proliferation and VEGF expression of human retinal pigment epithelial cells

International Journal of Ophthalmology(English edition) 2022年第6期15卷 905-913页

作者： Hai-Sheng Zheng Yu-qing lan Xing-Wu Zhong Huai-Sheng Zhou Jia-Yao Xu Hainan Eye Hospital and Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityHaikou 570311Hainan ProvinceChina Sun Yat-sen Memorial Hospital Sun Yat-sen UniversityGuangzhou 510120Guangdong ProvinceChina Foshan Second People’s Hospital Foshan 528000Guangdong ProvinceChina

AIM:To investigate the effects of curcumin(Cur)nanoparticles loaded with chitosan derivatives grafted by deoxycholic acid(Chit-DC)on human retinal pigment epithelial(h RPE)cell proliferation and vascular endothelial growth factor(VEGF)m RNA ***:Cur nanoparticles were synthesized with Chit-DC as the carrier and Cur as the supported *** counting kit-8(CCK-8)method was used to detect the effects of different concentrations of Cur/Chit-DC,Chit-DC,and Cur on the proliferation of h RPE cells for different *** changes of Cur/Chit-DC and Cur on h RPE cell cycle were determined by flow ***-quantitative reverse transcription-polymerase chain reaction(RT-PCR)was used to detect the m RNA expression levels of VEGF in h RPE cells treated with Cur,Chit-DC and Cur/Chit-DC at 10μg/m L for 24 ***:Different concentrations of Chit-DC nanoparticle treated h RPE cells had no significant difference in terms of optical density(OD)values compared with the control group at 24 h and 48 ***,there was no change in the cell morphology under a light *** 24 h treatment with Cur/Chit-DC and Cur,the percentage of G0-G1 phase cells increased and the percentage of S phase cells decreased in all concentration ***/Chit-DC and Cur in all concentration groups inhibited the proliferation of h RPE cells in a time and dose dependent manner,and reduced the expression level of VEGF m ***:The Cur/Chit-DC nanoparticles can release Cur continuously and have sustained release *** Cur/Chit-DC nanoparticles and Cur could inhibit h RPE cells cultured in vitro,and could reduce the expression level of VEGF m RNA in h RPE cells.

关键词： curcumin retinal pigment epithelial cells vascular endothelial growth factor cell counting kit-8 polymerase chain reaction

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Quantification of the presigmoid transpetrosal keyhole approach to petroclival region

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Chinese Medical Journal 2008年第8期121卷 740-744页

作者： WU Chen-yi lan qing Department of Neurosurgery Second Affiliated Hospital Suzhou University Suzhou Jiangsu 215004 China

Background Despite the presigmoid transpetrosal approach has been used by different researchers in various ways, the surgical injury rate remains high. Applying a minimally invasive keyhole idea, we devised a presigmoid transpetrosal keyhole approach （PTKA）, classified and quantitatively assessed their approach to the petroclival area on a cadaver model by using a neuronavigation system. Methods The presigmoid transpetrosal keyhole approach was divided into four increasingly morbidity-producing steps： retrolabyrinthine, partial labyrinthectomy with petrous apicectomy, translabyrinthine and transcochlear keyhole approaches. Six latex-injected cadaveric heads （twelve sides） underwent dissection in which a neuronavigation system was used. An area of exposure 10 cm superficial to a central target （working area） was calculated. The area of clival exposure with each subsequent dissection was also calculated. Results The retrolabyrinthine keyhole approach （RLK） spares hearing and facial function in theory but provides for only a small window of upper clival exposure. The view afforded by partial labyrinthectomy with petrous apicectomy keyhole approach （PLPAK） provides for up to four times this exposure. The translabyrinthine keyhole approach （TLK） and transcochlear keyhole approach （TCK）, although producing more morbidity, add little in terms of a larger petroclival window. However, with each step, the surgical freedom for manipulation of instruments increases. Conclusions The presigmoid transpetrosal keyhole approach to the petroclival area is feasible and useful. The RLK has relatively limited utility. For lesions without bone invasion, the PLPAK provides a much more versatile exposure with an excellent chance of hearing and facial nerve preservation. The TLK provides for greater versatility in treating lesions but clival exposure is not greatly enhanced. The TCK adds little in terms of intradural exposure but should be reserved for cases in which access to the pet

关键词： microsurgery neuronavigation skull base keyhole approaches

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河南省某医疗机构儿童家长抗菌药物使用知识、态度和行为的横断面调查分析

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中国合理用药探索 2024年第3期21卷 101-111页

作者：马伟峰王彩莲刘青蓝黄春芝赵庆贺任艳丽郑州大学第三附属医院(河南省妇幼保健院) 郑州450052 郑州市第一人民医院郑州450004

目的:本研究旨在探讨河南省某医疗机构儿童家长对抗菌药物使用知识、态度和行为的现状,为医疗机构制定抗菌药物管理政策提供参考。方法:2020年10~12月在河南省某医疗机构对住院患儿家长进行抗菌药物合理使用方面的横断面调查并对数据进... 详细信息

目的:本研究旨在探讨河南省某医疗机构儿童家长对抗菌药物使用知识、态度和行为的现状,为医疗机构制定抗菌药物管理政策提供参考。方法:2020年10~12月在河南省某医疗机构对住院患儿家长进行抗菌药物合理使用方面的横断面调查并对数据进行统计分析。结果:在289名受访者中,家长对抗菌药物使用知识情况总体良好,性别、受教育程度、医疗保障状况、子女数量、月收入和居住地与家长抗菌药物知识得分的关系最为密切(P<0.05)。家长对抗菌药物使用态度总体合格,其中月收入、性别、受教育程度、医疗保障状况和年龄是影响家长抗菌药物态度得分的因素(P<0.05)。家长的抗菌药物使用行为总体表现良好,其中影响家长抗菌药物行为得分的因素包括受教育程度、性别、年龄、与儿童的关系和医疗保障状况(P<0.05)。结论:尽管该院儿童家长对抗菌药物的使用持积极的态度和行动,但仍有部分家长对抗菌药物缺乏足够的了解,甚至对抗菌药物的使用存在误解。建议该院进一步加强对儿童家长抗菌药物知识的宣教,促进儿童抗菌药物的合理使用。

关键词：河南省儿童家长抗菌药物知识态度行为

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Minimally invasive keyhole approaches for removal of tumors of the third ventricle

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Chinese Medical Journal 2006年第17期119卷 1444-1450页

作者： lan qing DONG Jun HUANG Qiang Department of Neurosurgery Second Affiliated Hospital SuzhouUniversity Suzhou 215004 China

Background In recent years, keyhole microsurgery has become an important subject of modern minimally invasive neurosurgery. In this study, minimally invasive techniques avoiding unnecessary tissue injuries were applied to refine traditional approaches for the removal of third ventricular tumors within a limited operative filed. Methods Individualized keyhole approaches were designed according to the characteristics of third ventricular tumors and their growth patterns. A series of keyhole approaches such as supraorbital subfrontal approach, infratentorial supracerebellar approach, interhemispheric transcallosal approach, pterional approach were taken to enter the third ventricle anteriorly, posteriorly, superiorly or laterally, respectively. A total of 34 removed tumors in or extended into the third ventricle included 11 craniopharyngiomas, 10 pituitary adenomas, 2 pinealomas, 1 cholesteatoma, 3 germinomas, and 7 gliomas. Results Total tumor resection was done in 27 （79.4%） of the patients, and subtotal resection in 7 patients （20.6%）. Residual tumor was due to tight adherence of germinoma to the vein of Galen （1 patient）, craniopharyngioma to the pituitary stalk （3）, supratentorial extension of pineal region gliomas （1）, suprasellar extension of gliomas （1） and giant pituitary adenoma （1）. Complications such as brain contusion, postoperative hemorrhage and infection were not associated with keyhole approaches. Extended incision or enlarged bone flap was not made because of episode during operation or inadequate exposure. Conclusions Keyhole approaches are safe, effective and minimally invasive in the surgical treatment of tumors deeply seated in the third ventricle. Individualized keyhole approach ensures a successful treatment. Tumors in the upper middle part of the third ventricle can be exposed by the interhemispheric transcallosal keyhole approach. Tumors of the posterior third ventricle may be well exposed by the infratentorial supracerebellar keyhole ap

关键词： tumors, third ventricle microsurgery keyhole approaches

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Two pairs of unusual scalemic enantiomers from Isatis indigotica leaves

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Chinese Chemical Letters 2016年第12期27卷 1745-1750页

作者： Da-Wei Li qing-lan Guo Xian-Hua Meng Cheng-Gen Zhu Cheng-Bo Xu Jian-Gong Shi State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College

Two pairs of unusual scalemic enantiomers with a dimethoxyphenyl）ethyl]-2-methoxyindolin-3-one, novel carbon skeleton of 2-[1′-（4＂-hydroxy-3＂,5＂- named isatidifoliumindolinones A-D （1-4）, were isolated from an aqueous extract of lsatis indigotica leaves （da qing ye）. Their structures including absolute configurations were determined by spectroscopic data analysis combined with comparison of their experimental CD and calculated ECD spectra. Validity of the ECD spectra calculation to assign the absolute configurations is discussed. Plausible biosynthetic pathways of 1-4 are proposed. Stereochemistry-dependent activity against LPS-induced NO production in BY2 cells was observed, and among the stereoisomers compound 4 is most active.

关键词： Isatis indigotica Cruciferae 2-[ 1′-( 4″-Hydroxy-3″, 5″-dimethoxyphenyl)ethyl]-2-methoxyindolin-3-one Scalemic enantiomerlsatidifoliumindolinones A-D Bioactivity

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Gastradefurphenol,a minor 9,9＇-neolignan with a new carbon skeleton substituted by two p-hydroxybenzyls from an aqueous extract of “tian ma”

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Chinese Chemical Letters 2017年第6期28卷 1185-1189页

作者： Xue Zhou qing-lan Guo Cheng-Gen Zhu Cheng-Bo Xu Ya-Nan Wang Jian-Gong Shi State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College

Gastradefurphenol（1）,a minor 9.9＇-neolignan with a novel carbon skeleton substituted by two phydroxybenzyls was isolated from an aqueous extract of the Gastrodia elata rhizomes（tian ma）.Its structure was determined by extensive spectroscopic data ***-dependent free rotational restriction of bonds and equilibrium of two molecular states are discussed by comparison of the NMR spectroscopic data of 1 in acetone-d6 with those in CD3OD.A biogenetic pathway of 1 is postulated to be associated with metabolic processes of L-tyrosine.

关键词： Gastrodia elata Orchidaceae 9 9'-Neolignan Gastradefurphenol Minor constituent

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Gastrolatathioneine,an unusual ergothioneine derivative from an aqueous extract of "tian ma":A natural product co-produced by plant and symbiotic fungus

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Chinese Chemical Letters 2016年第10期27卷 1577-1581页

作者： qing-lan Guo Sheng Lin Ya-Nan Wang Cheng-Gen Zhu Cheng-Bo Xu Jian-Gong Shi State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College

Gastrolatathioneine （1）, an unusual natural product derived from ergothioneine, a fungal amino acid containing an imidazole-2-thione moiety, was isolated from an aqueous extract of ＂tian ma＂（the Gastrodia elata rhizomes）. The structure of 1 including the absolute configuration was determined by extensive spectroscopic data analysis, combined with comparison of an experimental circular dichroism spectrum and calculated electronic circular dichroism spectra of stereoisomers, and confirmed by X-ray crystallography. The natural origin of 1 was proved by HPLC-ESIMS analysis of the crude extract. A biogenetic pathway of I is proposed on the basis of metabolic post-modification of ergothioneine that is biosynthesized by a symbiotic fungus. The plant and symbiotic fungus are co-uroducers of 1.

关键词： Gastrodia elataOrchidaceaeErgothioneine(+)-L-N N-bis(4-hydroxybenzyl)ergothioneineGastrolatathioneine

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青年原发性高血压病中医证型与相关因素分析

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中国中西医结合杂志 2024年第9期44卷 1063-1071页

作者：李晓雅张静怡胡岚清孙长鑫张晓囡陈纪烨刘龙涛中国中医科学院西苑医院心血管科北京100091 国家中医心血管病临床医学研究中心北京100091 北京中医药大学研究生院北京100029

目的探讨青年原发性高血压病中医证型与相关因素间的关系。方法采用横断面研究设计,收集2017年12月—2021年12月中国中医科学院西苑医院心血管科住院患者中符合纳排标准的病例资料,通过Logistic回归分析方法探讨相关关系。结果研究共纳... 详细信息

目的探讨青年原发性高血压病中医证型与相关因素间的关系。方法采用横断面研究设计,收集2017年12月—2021年12月中国中医科学院西苑医院心血管科住院患者中符合纳排标准的病例资料,通过Logistic回归分析方法探讨相关关系。结果研究共纳入202例青年高血压病住院病例,中医证型分布依次为痰瘀互结证(51.98%)、阴虚阳亢证(24.75%)、气血两虚证(15.84%)、肾阳亏虚证(7.43%)。Logistic回归分析显示,脑梗死病史、高血压病分期、低密度脂蛋白胆固醇(LDL-C)水平、24 h平均收缩压(24 h ASBP)、24 h平均脉搏(24 h AP)、日间平均脉搏(dAP)、夜间平均脉搏(n AP)对气血两虚证有影响(P<0.05);高血压病分期、脂蛋白B(Apo B)水平、最高收缩压(max-SBP)对肾阳亏虚证有影响(P<0.05);脂肪肝病史、高密度脂蛋白胆固醇(HDL-C)降低、LDL-C升高、糖化血红蛋白(HbA1c)升高、估算肾小球滤过率(eGFR)降低、24 h AP、dAP对痰瘀互结证有影响(P<0.05);年龄、动脉粥样硬化、LDL-C降低、Apo B降低、HbA1c升高、夜间平均舒张压(nADBP)、舒张压时间升高百分比(PTE%-DBP)对阴虚阳亢证有影响(P<0.05)。结论青年高血压病住院患者中痰瘀互结证最多,中医证型与年龄、既往史、高血压病分期、相关血清学指标及动态血压指标之间存在一定的相关关系。

关键词：青年高血压病中医证型相关因素 Logistic回归分析中西医结合

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Napelline-type C_(20)-diterpenoid alkaloid iminiums from an aqueous extract of ‘‘fu zi'': Solvent-/base-/acid-dependent transformation and equilibration between alcohol iminium and aza acetal forms

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Chinese Chemical Letters 2016年第7期27卷 993-1003页

作者： Xian-Hua Meng Zhi-Bo Jiang Cheng-Gen Zhu qing-lan Guo Cheng-Bo Xu Jian-Gong Shi State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College

Three new napelline-type C20-diterpenoid alkaloids,named aconicarmichinium A and B trifluoroacetates（1 and 2） and aconicarmichinium C chloride（3）,were isolated from an aqueous extract of ＂fu zi＂,the lateral roots of Aconitum *** structures were elucidated by extensive spectroscopic data *** 1-3 represent the first examples of napelline-type C20 diterpenoid alkaloid alcohol iminiums,of which the structures were fully *** addition,transformation and equilibration between the alcohol iminiums（1-3） and the aza acetals la-3a were investigated by measurements of the NMR spectra in protic and aprotic deuterium solvents including alkali pyridine-d5,along with evaporation under reduced pressure and gradual additions of TFA,AcOH,and *** results demonstrated that the transformation and equilibration were solvent-,base-,and ***,in aqueous biological fluid,these C20-diterpenoid alkaloids would more likely exist as the alcohol iminiums accompanied by anion counterparts in biosystems to increase their solubility, bioavailability, transportations, and *** absolute configurations of 1-3 were confirmed by X-ray crystallographic analysis of 2a.

关键词： Aconitum carmichaelii Ranunculaceae Napelline-type C20-diterpenoid alkaloid Aconicarmichiniums A–C Alcohol iminium and aza acetal forms of the diterpenoid alkaloid Transformation and equilibration

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Apolipoprotein E polymorphisms increase the risk of post-stroke depression

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Neural Regeneration Research 2016年第11期11卷 1790-1796页

作者： Xue-bin Li Jie Wang An-ding Xu Jian-min Huang lan-qing Meng Rui-ya Huang Jun-li Wang Stroke Center & Neurology Division the First Affiliated Hospital of Jinan University Department of Nephrology the Affiliated Hospital of Youjiang Medical University for Nationalities Department of Neurology the Affiliated Hospital of Youjiang Medical University for Nationalities Department of Laboratory Medicines the Affiliated Hospital of Youjiang Medical University for Nationalities

Recent reports have shown that apolipoprotein E （APOE） polymorphisms are involved in neurodegenerative disease. However, it is unclear whether APOE affects post-stroke depression. Accordingly, we hypothesized that APOE polymorphisms modify the risk of post-stroke depression. Here, we performed a hospital-based case-control study （including 76 cerebral infarction cases with post-stroke depression, 88 cerebral infarction cases without post-stroke depression, and 109 controls without any evidence of post-stroke depression or cerebral infarction） to determine possible association between APOE rs429358 and rs7412 polymorphisms and risk of post-stroke depression. Our findings show no difference among the groups with regards genotype distribution of the rs7412 polymorphism. In contrast, APOE genotypes with rs429358-C alleles increased the risk of post-stroke depression. Further, the rs429358 polymorphism was associated with significantly decreased regional cerebral blood flow values in the left temporal lobe of post-stroke depression cases. Additionally, the rs429358 polymorphism was not only associated with depression severity, but with increasing serum levels of total cholesterol. These resuits suggest that the APOE rs429358 polymorphism is associated with increased risk of developing post-stroke depression, and that APOE rs429358-C allele genotypes may be detrimental to recovery of nerve function after stoke. Indeed, these findings provide clinical data for future post-stroke depression gene interventions.

关键词： nerve regeneration apolipoprotein E genetic polymorphism post-stroke depression risk regional resting-state cerebral blood flow rs429358 rs7412 cerebral infarction neural regeneration

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