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Chemical Research in Chinese Universities 2007年第6期23卷 688-692页

作者： HOU Xia WU qing-tian ZHANG Yu-ping ZHU Na LIU Yan-li FENG Xue-chao MA Tong-hui Membrane Channel Research Laboratory Northeast Normal University Changchun 130024 P. R. China School of Basic Medicine Jiamusi University Jiamusi 154002 P. R. China

mCLCA3 is a member of calcium activated chloride channel（CACC） family that may play an important role in mucin packaging and secretion in asthmatic and cystic fibrosis lung. To study the protein structure and expression of mCLCA3 in asthmatic mouse lung, an N-terminal 269 amino acid peptide of mCLCA3 was expressed in E. coli, purified to homogeneity and rabbit polyclonal antibodies against this peptide were generated. Immunohistochemistry of asthmatic mouse lung using the antibody indicated exclusive mCLCA3 expression in mucin granules of goblet cells in airway surface and lumen, Immunoblot analysis of lavage fluid from asthmatic mouse lung revealed a single 90 kDa protein form of mClCA3. The results demonstrate that the 90 kDa N-terminal peptide, neither the flail-length protein nor the reported N-terminal 35 kDa cleaved form of mClCA3 is the major functional form involved in the packaging and exocytosis of mucin granules in asthmatic goblet cells.

关键词： mCLCA3 Prokaryotic expression Purification Antibody production Asthma

Tungsten and arsenic substance flow analysis of a hydrometallurgical process for tungsten extracting from wolframite

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Tungsten 2021年第3期3卷 348-360页

作者： Yuan-Lin Chen Xue-Yi Guo Qin-Meng Wang qing-Hua tian Shao-Bo Huang Jin-Xiang Zhang School of Metallurgy and Environment Central South UniversityChangsha 410083China Changsha Research Institute of Mining and Metallurgy Co.LTD Changsha 410012China Ganzhou Nonferrous Metallurgy Research Institute Ganzhou 341000China

In this study,the metabolism of a hydrometallurgical process for tungsten extracting from wolframite was studied through substance flow *** mass balance accounts,substance flow charts of tungsten and arsenic were established to evaluate the metabolism efficiency of the investigated *** results showed that,the total tungsten resource efficiency of the system was 97.56%,and the tungsten recovery of unit process autoclaved alkali leaching,ion exchange,Mo removing,concentration and crystallization was 98.16%,98.94%,99.71%,99.89%,***,for extracting 1 ton of tungsten into the qualified ammonium paratungstate,10.0414 kg of arsenic was carried into the system,with the generation of 7.2801 kg of arsenic in alkali leaching residue,1.5067 kg of tungsten in arsenic waste residue,and 1.2312 kg of tungsten in Mo ***,7.9 g of arsenic was discharged into nature environment with waste water,15.5 g of arsenic was entrained into the final *** distribution and transformation behaviors of arsenic during production were analyzed through phases change analysis,and some recommendations for improving the resource efficiency of tungsten and pollution control during production were also proposed based on the substance flow analysis in this study.

关键词： Tungsten Arsenic Substance flow analysis Resource efficiency Pollution control

Optimal spectral phase control of femtosecond laser-induced up-conversion luminescence in Sm^3+:NaYF4 glass

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Frontiers of physics 2020年第2期15卷 63-68页

作者： Jian-Ping Li Lian-Zhong Deng Ye Zheng Peng-Peng Ding tian-qing Jia Zhen-Rong Sun Jian-Rong Qiu Shi-An Zhang State Key Laboratory of Precisicm Spectroscopy School of Physics and Electronic ScienceEast China Normal UniversityShanghai 200062China State Key Laboratory of Modem Optical Instrumentation College of Optical Science and EngineeringZhejiang UniversityHangzhou 310027China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China

The spectral phase of the femtosecond laser field is an important parameter that affects the up-conversion(UC)luminescence efficiency of dopant lanthanide *** this work,we report an experi-mental study on controlling the UC lmiiinescence efficiency in Sm^3+:NaYF4 glass by 800-nm femtosec-ond laser pulse shaping using spectral phase *** optimal phase control strategy efficiently enhances or suppresses the UC luminescence *** on the laser-power dependence of the UC luminescence intensity and its comparison with the luminescence spectrum under direct 266-nm fem-tosecond lciser irradiation,we propose herein an excitation model combining non-resonant two-photon absorption with resonance-media ted three-photon absorption to explain the experimental observations.

关键词： up-conversion luminescence rare earth ions quantum control femtosecond laser spectral phase

VPS4B mutation impairs the osteogenic differentiation of dental follicle cells derived from a patient with dentin dysplasia type I

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International Journal of Oral Science 2020年第3期12卷 220-226页

作者： Qiang Li Fangli Lu tianxuan Chen Ke Zhang Yuping Lu Xiaocong Li Yingying Wang Ling Liu qing tian Fu Xiong Dong Chen Department of Stomatology the First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina The Academy of Medical Sciences Zhengzhou UniversityZhengzhouChina Department of Stomatology Henan Provincial HospitalZhengzhouChina Implant Dental Clinic ZhengzhouChina Department of Stomatology Puyang People’s HospitalPuyangChina Department of Medical Genetics School of Basic Medical SciencesSouthern Medical UniversityGuangzhouChina

A splicing mutation in VPS4B can cause dentin dysplasia type I(DD-I),a hereditary autosomal-dominant disorder characterized by rootless teeth,the etiology of which is genetically *** our study,dental follicle cells(DFCs)were isolated and cultured from a patient with DD-I and compared with those from an age-matched,healthy *** a previous study,this DD-I patient was confirmed to have a loss-of-function splicing mutation in VPS4B(IVS7+46C>G).The results from this study showed that the isolated DFCs were vimentin-positive and CK14-negative,indicating that the isolated cells were derived from the *** harboring the VPS4B mutation had a significantly higher proliferation rate from day 3 to day 8 than control DFCs,indicating that VPS4B is involved in cell *** cells were then replenished with osteogenic medium to investigate how the VPS4B mutation affected osteogenic *** of osteogenesis,detected by alizarin red and alkaline phosphatase staining in vitro,was decreased in the DFCs from the DD-I patient compared to the control ***,we also found that the VPS4B mutation in the DD-I patient downregulated the expression of osteoblast-related genes,such as ALP,BSP,OCN,RUNX2,and their encoded *** outcomes confirmed that the DD-I-associated VPS4B mutation could decrease the capacity of DFCs to differentiate during the mineralization process and may also impair physiological root formation and bone *** might provide valuable insights and implications for exploring the pathological mechanisms underlying DD-I root development.

关键词： patient dysplasia matched

Effect of melting rate on microsegregation and primary MC carbides in M2 high-speed steel during electroslag remelting

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China Foundry 2021年第3期18卷 163-169页

作者： Fu-xing Yin Ming Su Fa Ji qing-chao tian Ya-guan Bai Jian-hang Feng Zhi-xia Xiao School of Materials Science and Engineering Hebei University of TechnologyTianjin 300130China tianjin Key Laboratory of Layered Composite Materials Tianjin 300130China State Key Laboratory of Advanced Special Steel Shanghai Key Laboratory of Advanced FerrometallurgySchool of Materials Science and EngineeringShanghai UniversityShanghai 200444China tianjin Heavy Industries Research and Development Co. Ltd.Tianjin 300457China

Large-size primary MC carbides can significantly reduce the performance of M2 high-speed *** better control the morphology and size of primary MC carbides,the effect of melting rate on microsegregation and primary MC carbides of M2 steel during electroslag remelting was *** the melting rate is decreased from 2 kg·min^(-1) to 0.8 kg·min^(-1),the columnar dendrites are gradually coarsened,and the extent of segregation of Mo and V is alleviated,while the segregation of Cr becomes *** 2 kg·min^(-1),the number of primary MC carbides per unit area with the sizes in the range of 2 μm to 6 μm accounts for about 75% of all MC carbides,while the carbides are mainly concentrated on the size larger than 8 μm at 0.8 kg·min^(-1).Thermodynamic calculations based on the Clyne-Kurz (simplified to C-K) model shows that MC carbide can be precipitated in the final solidification stage and a smaller secondary dendrite arm spacing caused by higher melting rate (2 kg·min^(-1) in this experiment) facilitates the refinement of primary MC carbides.

关键词： high-speed steel MC carbide melting rate electroslag remelting microsegregation

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公路高大加筋土挡墙设计方法的研究

中国公路学报 2000年第2期13卷 13-15,19页

作者：凌天清重庆交通学院道路工程系重庆400074

在室内模型试验和野外 4 3.75m原型墙测试研究的基础上 ,根据理论分析提出了高大加筋土挡墙的合理破裂面形式、筋带拉力计算式与面板后侧压力、垂直土压力与筋带锚固长度等计算方法 ,据此编制了计算分析的

关键词：高填土加筋土挡墙土压力筋带长度公路

Reducing structural degradation of high-voltage single-crystal Ni-rich cathode through in situ doping strategy

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Rare Metals 2023年第9期42卷 2993-3003页

作者： Xin-Ming Fan Zhi Zhang Gao-Qiang Mao Ying-Jie Tong Ke-Bo Lin Hui Tong Wei-Feng Wei qing-Hua tian Xue-Yi Guo Powder Metallurgy Research Institute Central South UniversityChangsha410083China School of Metallurgy and Environment Central South UniversityChangsha410083China National&Regional Joint Engineering Research Center of Nonferrous Metal Resource Recycling Changsha410083China Engineering Research Center of the Ministry of Education for Advanced Battery Materials Central South UniversityChangsha410083China College of Chemistry and Materials Engineering Hunan University of Arts and ScienceChangde415000China

Polycrystalline Ni-rich layered oxide (Li Ni_(x)Co_(y)Mn_zO_(2)(NCM),x>0.8) cathode material with high specific capacity and low cost is considered as one of the most promising candidate materials for lithium-ion batteries (LIBs).However,it suffers from severe structural and capacity degradation during practical cycling,especially under harsh operation condition(ultrahigh cutoff voltage and elevated temperature,etc.).One promising approach to mitigate these issues is to develop a single-crystal Ni-rich NCM cathode,which could enhance structural integrity and improve capacity retention,due to its robust and stable micro-sized primary ***,the improved cyclic stability comes at the expense of reversible capacity and rate capability,owing to the relatively low Li^(+) diffusion efficiency for its micron-sized primary ***,the structural degradation and exacerbation of interfacial reactions for the Ni-rich NCM cathode under highvoltage (≥4.5 V) would quickly trigger the poor electrochemical performance,limiting its practical ***,Li Ni_(0.827)Co_(0.11)Zr_(0.003)Mn_(0.06)O_(2)(Zr@SC-N_(83)) cathode material was successfully synthesized via the in situ doping *** could not only effectively maintain the reversibility of phase transition between H2 and H3 after long-term cycling at high voltage (4.6 V),but also enhance lithium-ion diffusion,thus improving the cycling performance and good rate performance for the Zr@SC-N_(83)*** a result,0.3 wt%Zrdoping cathode delivers an initial discharging capacity of 200.1 m Ah·g^(-1)at 1.0C and at the high cutoff voltage of 4.6 V,exhibiting the satisfactory capacity retention of 85.5%after *** provides an effective route toward low-cost and higher energy density for lithium-ion batteries with Ni-rich cathode.

关键词： Single-crystal Ni-rich LiNi_(x)Co_(y)Mn_zO_(2)(NCM) In situ doping strategy High voltage Structural integrity Cycling stability

On Buddhism and Chinese Music

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The Voice of Dharma 2020年第1期1卷 89-105,182-183页

作者： tian qing Hu Mengdi(译) Music Research Institute of the Chinese National Academy of Arts Center for Religious Art Research of the Chinese National Academy of Arts China Kun Opera and Guqin Research Institute

Influence of Buddhism on Traditional Chinese Culture If we observe traditional Chinese music from the perspective of the sociology of music instead of the morphology of music,and if we view music as a way of thinking and a cultural phenomenon instead of merely as a skill or a form,it is an appropriate way to classify Chinese traditional music into four categories,namely,folk music,court music,religious music,and literati ***,without the investigation into and research of Chinese religious music,we will fail to grasp the whole picture of Chinese traditional music.

关键词： religious classify his

Regional differences in rainfall frequency and amount over southwestern China

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Atmospheric and Oceanic Science Letters 2019年第5期12卷 313-319页

作者： YUAN Weihua HU Xuelin tian qing State Key Laboratory of Numerical Modeling for Atmospheric Sciences and Geophysical Fluid Dynamics Institute of Atmospheric PhysicsChinese Academy of SciencesBeijingChina College of Earth and Planetary Science University of Chinese Academy of SciencesBeijingChina Beijing Municipal Climate Center BeijingChina

In this paper,the rainfall features in southwestern China are studied using daily rainfall station *** rainfall features are distinct along the eastern and western edges of the Hengduan Mountains and over the mountains,especially in terms of rainfall *** rainfall amounts and frequencies are much higher along the eastern and western edges than over the mountains,particularly during spring,which is partly contributed by the number and duration of rainfall *** differences are more obvious in the nocturnal rainfall than in the daytime *** rainfall differences over the three regions could be affected by the large-scale *** analyzing reanalysis data,the large-scale circulations linked to the different rainfall features over southwestern China,and the interactions of these circulations with the topography are also discussed.

关键词： Rainfall frequency rainfall amount southwestern China regional differences

Heme status affects human hepatic messenger RNA and microRNA expression

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World Journal of Gastroenterology 2013年第10期19卷 1593-1601页

作者： Herbert L Bonkovsky Weihong Hou Nury Steuerwald qing tian Ting Li Judy Parsons Alicia Hamilton Sunil Hwang Laura Schrum the Liver Digestive and Metabolic Disorders LaboratoryCannon Research CenterCarolinas Medical Center1542 Garden TerraceCharlotteNC 28203United States the Molecular Core Laboratory Cannon Research CenterCarolinas Medical CenterCharlotteNC 28203United States

AIM:To assess effects of heme on messenger RNA(mRNA) and microRNA(miRNA) profiles of liver cells derived from ***:We exposed human hepatoma cell line Huh-7 cells to excess iron protoporphyrin(heme)(10 μmol/L) or induced heme deficiency by addition of 4,6-dioxoheptanoic acid(500 μmol/L),a potent inhibitor of aminolevulinic acid dehydratase,for 6 h or 24 *** harvested total RNA from the cells and performed both mRNA and miRNA array analyses,with use of Affymetrix chips,reagents,and instruments(human genome U133 plus 2.0 and miRNA 2.0 arrays).We assessed changes and their significance and interrelationships with Target Scan,Pathway Studios,and Ingenuity ***:Changes in mRNA levels were most numerous and striking at 6 h after heme treatment but were similar and still numerous at 24 *** 6 h of heme exposure,the increase in heme oxygenase 1 gene expression was 60-fold by mRNA and 88-fold by quantitative reverse transcription-polymerase chain *** found striking changes,especially up-regulation by heme of nuclear erythroid-2 related factor-mediated oxidative stress responses,protein ubiquitination,glucocorticoid signaling,P53 signaling,and changes in RNAs that regulate intermediary *** mRNAs were down-regulated by heme,and the fold decreases were less exuberant than were the *** decreases after 24 h of heme exposure were patatin-like phospholipase domain-containing protein 3(-6.5-fold),neuronal PAS domain protein 2(-1.93-fold),and protoporphyrinogen oxidase(-1.7-fold).CONCLUSION:Heme excess exhibits several toxic effects on liver and kidney,which deserve study in humans and in animal models of the human porphyrias or other disorders.

关键词： Delta-aminolevulinic acid synthase Heme Heat shock proteins Hepatotoxicity Messenger RNA MicroRNA