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The different interactions of Colletotrichum gloeosporioides with two strawberry varieties and the involvement of salicylic acid

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Horticulture Research 2016年第1期3卷 277-286页

作者： qing-Yu Zhang Li-qing Zhang Li-Li Song Ke Duan Na Li Yan-Xiu Wang qing-Hua gao Shanghai Key Laboratory of Protected Horticultural Technology Forestry and Fruit Tree Research InstituteShanghai Academy of Agricultural Sciences(SAAS)Shanghai 201403China College of Landscape Architecture and Arts Northwest A&F UniversityYanglingShanxi 712100China College of Agricultural Sciences Gansu Agricultural UniversityLanzhou 730000China School of Life Science Taizhou UniversityTaizhou 318000China

The disease symptoms recognized as‘Anthracnose’are caused by Colletotrichum *** lead to large-scale strawberry(Fragaria×ananassa Duchesne)losses worldwide in terms of both quality and *** is known regarding the mechanisms underlying the genetic variations in the strawberry–Colletotrichum *** this work,Colletotrichum gloeosporioides(***)infection was characterized in two varieties exhibiting different susceptibilities,and the involvement of salicylic acid(SA)was *** microscopic observation showed that *** conidia germinated earlier and faster on the leaf surface of the susceptible cultivar compared with the less-susceptible *** PR genes were differentially expressed,with higher-amplitude changes observed in the less-susceptible *** less-susceptible cultivar contained a higher level of basal SA,and the SA levels increased rapidly upon infection,followed by a sharp decrease before the necrotrophic *** SA pretreatment reduced susceptibility and elevated the internal SA levels in both varieties,which were sharply reduced in the susceptible cultivar upon *** less-susceptible cultivar also displayed a more sensitive and marked increase in the transcripts of NB-LRR genes to ***,and SA pretreatment differentially induced transcript accumulation in the two varieties during ***,SA directly inhibited the germination of *** conidia;NB-LRR transcript accumulation in response to SA pretreatment was both dose-and *** results demonstrate that the less-susceptible cultivar showed reduced conidia *** contribution of SA might involve microbial isolate-specific sensitivity to SA,cultivar/tissue-specific SA homeostasis and signaling,and the sensitivity of R genes and the related defense network to SA and pathogens.

关键词： cultivar straw gloeosporioides

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Pressure Driven Magnetohydrodynamics Instabilities in Peaked Pressure Profile Reversed Magnetic Shear Plasmas

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Chinese Physics Letters 2001年第6期18卷 790-792页

作者： gao qing-Di ZHANG Jin-Hua QU Hong-Peng Southwestern Institute of Physics P.O.Box 432Chengdu 610041

For a reversed magnetic shear plasma formed by early neutral beam injection into the HL-2A tokamak,magnetohydrodynamics instability analysis against ideal low-n modes and resistive interchange modes is carried ***-n modes located in the low shear region around the shear reversal point are driven unstably by large pressure gradient,and they are of the characteristics of infernal *** pressure in the central negative shear region drives resistive interchange modes with the unstable window extending to r/a≈0.2,but not covering the low shear region around the shear reversal point.

关键词： reversed unstable Plasma

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Influence on Cellular Signal Transduction Pathway in Dairy Cow Mammary Gland Epithelial Cells by Galactopoietic Compound Isolated from Vaccariae segetalis

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Agricultural Sciences in China 2011年第4期10卷 619-630页

作者： WAN Zhong-ying TONG Hui-li LI qing-zhang gao Xue-jun Key Laboratory of Dairy Science Ministry of Education~Northeast Agricultural University Harbin 150030 P.R.China

The galactopoietic mechanism of Vaccaria segetalis is still unknown. Understanding dibutyl phthalate （DBP） separated from Vaccaria segetalis on the expression of lactation signal transduction genes of mammary gland epithelial cells, including prlr, erα, akt1, socs2, pparγ and elf5, will be helpful to reveal the molecular mechanism. Western blot and qRT- PCR were used to study the change of prlr, erα, akt, socs2, pparγ, and elf5 expression at mRNA and protein level. Co- localization expression of prolactin receptor （PRLR） and estrogen receptor α （ERα） was observed by immunofluorescence; the expression changes of miRNAs （21, 125b, 143, and 195） and the secretion of β-casein and lactose were detected by qRT-PCR and RP-HPLC. The results showed that Vaccaria segetalis active compound had similar fuctions as estrogen and/or prolactin （PRL） in dairy cow mammary gland epithelial cells （DCMECs）, increased the expressions of prlr, erα, akt1, and elf5 genes, while repressed pparγ expressions. DBP promoted socs2 mRNA expression, but its protein expressions were repressed. Furthermore, both DBP and PRL could repress the expressions of miRNA-125b, miRNA-143 and miRNA- 195 in DCMECs. DBP could repress the expression of miRNA-21, while the influence of PRL on miRNA-21 was not certain. DBP could promote the lactation ability of DCMECs by regulating the ER and PRLR cellular signal transduction pathway.

关键词： Vaccaria segetalis DBP dairy cow mammary gland epithelial cells signal transduction miRNAs

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Bounded Real Lemmas for Fractional Order Systems

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International Journal of Automation and computing 2015年第2期12卷 192-198页

作者： Shu Liang Yi-Heng Wei Jin-Wen Pan qing gao Yong Wang Department of Automation University of Science and Technology of China School of Engineering and Information Technology University of New South Wales at the Australian Defence Force Academy

This paper derives the bounded real lemmas corresponding to L∞norm and H∞norm(L-BR and H-BR) of fractional order systems. The lemmas reduce the original computations of norms into linear matrix inequality(LMI) problems, which can be performed in a computationally efficient fashion. This convex relaxation is enlightened from the generalized Kalman-YakubovichPopov(KYP) lemma and brings no conservatism to the L-BR. Meanwhile, an H-BR is developed similarly but with some ***, it can test the system stability automatically in addition to the norm computation, which is of fundamental importance for system analysis. From this advantage, we further address the synthesis problem of H∞control for fractional order systems in the form of LMI. Three illustrative examples are given to show the effectiveness of our methods.

关键词： Fractional order systems L∞norm H∞norm H∞control bounded real lemmas linear matrix inequality(LMI).

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Design of a compact athermalized infrared seeker

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Optoelectronics Letters 2017年第4期13卷 287-290页

作者： gao qing-jia WANG Jian SUN Qiang Changehun Institute Of Optics Fine Mechanics and Physics Chinese Academy of Science Changchun 130033 China University of Chinese Academy of Science Beijing 100049 China

In order to meet the application requirement of a certain long wavelength infrared （LWIR） seeker, a small volume, light weight and passively athermalized infrared OR） objective is designed in this paper. The two-lens telephoto struc- ture is adopted by merely using aluminum alloy as the housing material. By balancing the thermo-optical coefficient and thermal expansion coefficient of materials, an athermalized IR seeker with effective focal length of 90 mm and F number of 1.2 is achieved. The whole optical length is 75 ram, and the weight is only 234 g. The objective can remain fine imaging quality under temperature range from -40 ℃ to 60 ℃, which is beneficial to the lightweight design oflR seekers.

关键词： Aluminum alloys Thermal expansion

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Intra-seasonal Features of an Extreme High Temperature Event in 2011 in Eastern China and Its Atmospheric Circulation

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Journal of Tropical Meteorology 2021年第4期27卷 437-446页

作者： gao qing-jiu LI Yan HAN Tong-xin Key Laboratory of Meteorological Disaster Ministry of Education(KLME)Joint International Research Laboratory of Climate and Environment Change(ILCEC)and Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters(CIC-FEMD)Nanjing University of Information Science and TechnologyNanjing 210044 China

Based on the daily maximum air temperature(T_(max))data from the China Meteorological Data Network and the NCEP/DOE reanalysis data,the intra-seasonal circulation and evolution of an extreme high temperature event(EHTE)in the middle reaches of the Yangtze River(MYR)from August 9-21,2011 were explored,as well as the influence of diabatic heating on the position variation of the Western Pacific subtropical high(WPSH).Results show that the daily T_(max) in the MYR exhibits a vigorous intraseasonal oscillation(ISO)of 10-25 days in the extended summer of *** main factors affecting the EHTE in the summer of 2011 are the low-frequency wave train propagating southeastward in the mid-latitude of the upper troposphere and the low-frequency anticyclone moving northwestward in the lowlatitude of the mid-lower *** diagnosis of 925hPa thermodynamic equation indicates that the ISO features of the T_(max) in the core region is determined by the intra-seasonal variation of the adiabatic *** addition,the variations of the WPSH correspond well to the distribution of apparent heat *** the early stage of the high temperature process,the apparent heat source in the north of the Bay of Bengal is a certain indicator for the westward extension of the WPSH.

关键词： extreme high temperature event intra-seasonal oscillation diagnosis Western Pacific subtropical high complete form of vertical vorticity tendency equation

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Cracking Behaviour of Al_2O_3 Short Fibre/Al-5.5Zn Composite in TEM

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Journal of Materials Science & Technology 1997年第3期13卷 235-237页

作者： Xiyan ZHANG Tianxin TAN Wenyi PENG and qing gao (Southwest Jiaotong University, Chengdu 610031, China) Southwest Jiaotong University Chengdu 610031 China

The micromechanical behaviour of Al2O3 short fibre reinforced Al-5.5Zn matrix composite has been in-situ studied by using TEM. The results show that in the composite the nucleation and propagation of cracks take place mainly in the matrix, as well as in the region near the fibre/matrix interface. It has been also observed that the microcracks can close during *** the fracture property can be characterized by crack open displacement (COD). The cracking characteristic may result from the large strength difference between the matrix and fibre.

关键词： Cracking Behaviour of Al2O3 Short Fibre/Al-5.5Zn Composite in TEM Al Zn

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Quinoline-based aggregation-induced delayed fluorescence materials for highly efficient non-doped organic light-emitting diodes

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Chinese Chemical Letters 2021年第2期32卷 740-744页

作者： Liang Zhang Yin-Feng Wang Meng Li qing Yu gao Chuan-Feng Chen Beijing National Laboratory for Molecular Sciences CAS Key Laboratory of Molecular Recognition and FunctionInstitute of ChemistryChinese Academy of SciencesBeijing 100190China College of Chemical Engineering China University of Mining and TechnologyXuzhou 221116China University of Chinese Academy of Sciences Beijing W0049China

Three new emitters,namely 10,10’-(quinoline-2,8-diyl)bis(10 H-phenoxazine)(Fene),10,10’-(quinoline-2,8-diyl)bis(10 H-phenothiazine)(Fens) and 10,10’-(quinoline-2,8-diyl)bis(9,9-dimethyl-9,10-dihydroacridine)(Yad),featuring quinoline as a new electron acceptor have been designed and conveniently *** emitters possessed small singlet-triplet splitting energy(ΔEst) and twisted structures,which not only endowed them show thermally activated delayed fluorescence(TADF)properties but also afforded a remarkable aggregation-induced emission(AIE) ***,they also showed aggregation-induced delayed fluorescence(AIDF) property and good photoluminescence(PL) property,which are the ideal emitters for non-doped organic light-emitting diodes(OLEDs).Furthermore,high-performance non-doped OLEDs based on Fene,Fens and Yad were achieved,and excelle nt maximum external quantum efficiencies(EQEmax) of 14,9%,13.1% and 17,4%,respectively,were *** was also found that all devices exhibited relatively low turn-on voltages ranging from 3.0 V to3.2 V probably due to their twisted conformation and the AIDF *** results demonstrated the quinoline-based emitters could have a promising application in non-doped OLEDs.

关键词： Quinoline Aggregation-induced emission Thermally activated delayed fluorescence Non-doped organic light-emitting diode

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Sacrificial microgel‑laden bioink‑enabled 3D bioprinting of mesoscale pore networks

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Bio-Design and Manufacturing 2020年第1期3卷 30-39页

作者： Lei Shao qing gao Chaoqi Xie Jianzhong Fu Meixiang Xiang Zhenjie Liu Liulin Xiang Yong He State Key Laboratory of Fluid Power and Mechatronic Systems College of Mechanical EngineeringZhejiang UniversityHangzhou 310027China Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province College of Mechanical EngineeringZhejiang UniversityHangzhou 310027China Department of Cardiology The Second Affiliated Hospital of Zhejiang UniversitySchool of MedicineHangzhou 310009China Department of Vascular Surgery The Second Affiliated Hospital of Zhejiang UniversitySchool of MedicineHangzhou 310009China Zhejiang University Hospital Zhejiang UniversityHangzhou 310027ZhejiangChina Key Laboratory of Materials Processing and Mold Zhengzhou UniversityZhengzhou 450002China

Three-dimensional(3D)bioprinting is a powerful approach that enables the fabrication of 3D tissue constructs that retain complex biological ***,the dense hydrogel networks that form after the gelation of bioinks often restrict the migration and proliferation of encapsulated ***,a sacrificial microgel-laden bioink strategy was designed for directly bioprinting constructs with mesoscale pore networks(MPNs)for enhancing nutrient delivery and cell *** sacrificial microgel-laden bioink,which contains cell/gelatin methacryloyl(GelMA)mixture and gelled gelatin microgel,is first thermo-crosslinked to fabricate temporary predesigned cell-laden constructs by extrusion bioprinting onto a cold ***,the construct is permanently stabilized through photo-crosslinking of *** MPNs inside the printed constructs are formed after subsequent dissolution of the gelatin *** MPNs allowed for effective oxygen/nutrient diffusion,facilitating the generation of bioactive ***,osteoblast and human umbilical vein endothelial cells encapsulated in the bioprinted large-scale constructs(≥1 cm)with MPNs showed enhanced bioactivity during *** 3D bioprinting strategy based on the sacrificial microgel-laden bioink provided a facile method to facilitate formation of complex tissue constructs with MPNs and set a foundation for future optimization of MPN-based tissue constructs with applications in diverse areas of tissue engineering.

关键词： Sacrificial microgel Gelatin methacryloyl(GelMA) 3D bioprinting Mesoscale pore networks(MPNs) Tissue engineering

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Cryptic diversity and rampant hybridization in annual gentians on the qinghai-Tibet Plateau revealed by population genomic analysis

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Plant Diversity 2024年第2期46卷 194-205页

作者： Peng-Cheng Fu Qiao-Qiao Guo Di Chang qing-Bo gao Shan-Shan Sun School of Life Science Luoyang Normal UniversityLuoyang 471934PR China Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau BiologyChinese Academy of SciencesXining 810001PR China

Understanding the evolutionary and ecological processes involved in population differentiation and speciation provides critical insights into biodiversity formation. In this study, we employed 29,865 single nucleotide polymorphisms(SNPs) and complete plastomes to examine genomic divergence and hybridization in Gentiana aristata, which is endemic to the qinghai-Tibet Plateau(QTP) region. Genetic clustering revealed that G. aristata is characterized by geographic genetic structures with five clusters(West, East, Central, South and North). The West cluster has a specific morphological character(i.e., blue corolla) and higher values of FSTcompared to the remaining clusters, likely the result of the geological barrier formed by the Yangtze River. The West cluster diverged from the other clusters in the Early Pliocene;these remaining clusters diverged from one another in the Early Quaternary. Phylogenetic reconstructions based on SNPs and plastid data revealed substantial cyto-nuclear conflicts. Genetic clustering and D-statistics demonstrated rampant hybridization between the Central and North clusters,along the Bayankala Mountains, which form the geological barrier between the Central and North clusters. Species distribution modeling demonstrated the range of G. aristata expanded since the Last Interglacial period. Our findings provide genetic and morphological evidence of cryptic diversity in G. aristata, and identified rampant hybridization between genetic clusters along a geological *** findings suggest that geological barriers and climatic fluctuations have an important role in triggering diversification as well as hybridization, indicating that cryptic diversity and hybridization are essential factors in biodiversity formation within the QTP region.

关键词： Gentiana aristata Hybrid zone qinghai-Tibet Plateau Plastome Nuclear SNPs

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