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High-throughput genotyping of single-nucleotide polymorphisms in ace-1 gene of mosquitoes using MALDI-TOF mass spectrometry

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Insect Science 2013年第2期20卷 167-174页

作者： Yun Mao Feng Tan Shuai-Guo Yan Guo-Xing Wu Chuan-ling qiao Wen-Xue Zhang Feng Cui College of Life Sciences Henan Normal University Xinxiang Henan Province State Key Laboratory of Integrated Management of Pest Insects & Rodents Institute of Zoology Chinese Academy of Sciences Beijing College of Plant Protection Yunnan Agricultural University Kunming China

Acetylcholinesterase （ACHE） plays a vital role in the nervous system of insects and other animal species and serves as the target for many chemical agents such as organophosphate and carbamate insecticides. The mosquito, Culex pipiens complex, a vector of human disease, has evolved to be resistant to insecticides by a limited number of amino acid substitutions in ACHE1, which is encoded by the ace-1 gene. The aims of this study are to identify single nucleotide polymorphism （SNP） sites in the ace-1 gene of the C. pipiens complex and explore an economical high-throughput method to differentiate the genotypes of these sites in mosquitoes collected in the field. We identified 22 SNP sites in exon regions of the ace-1 gene. Four of them led to non-synonymous mutations, that is, Y163C, G247S, C677S and T682A. We used matrix-assisted laser desorption ionization - time-of-flight mass spectrometry for genotyping at these four sites and another site F416V, which was relevant to insecticide resistance, in 150 mosquitoes collected from 15 field populations. We were able to synchronize analysis of the five SNP sites in each well of a 384-well plate for each individual mosquito, thus decreasing the cost to one-fifth of the routine analysis. Heterozygous genotypes at Y163C and G247S sites were observed in one mosquito. The possible influence of the five SNP sites on the activity or function of the enzyme is discussed based on the predicted tertiary structure of the enzyme.

关键词： acetylcholinesterase genotype insecticide resistance MassArray SNP

Hepatitis B virus X protein and C/EBPs cooperate in the activation of SREBP-1a transcription

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Hepatitis B virus X protein and C/EBPs cooperate in the acti...

Amplicon-Based Illumina Sequencing and Quantitative PCR Reveals Nanoplankton Diversity and Biomass in Surface Water of Qinhuangdao Coastal Area, China

中华医学会第十二次全国内分泌学学术会议

作者： ling qiao 2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics Key Laboratory of Hormones and Development(Ministry of Health)Metabolic Diseases Hospital & Tianjin Institute of EndocrinologyTianjin Medical University

BACKGROUND & AIMS HBx is well-known to be a multifunctional protein which is an important regulator of hepatic steatosis observed in patients with hepatitis B *** regulatory element binding protein-1a(SREBP-1a) is a potent activator of genes involved in the synthesis of cholesterol,fatty acids and *** previously study showed that HBx

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Journal of Ocean University of China 2019年第4期18卷 962-976页

作者： qiao ling YU Jie LI Ying GUO Haobing ZHEN Yu ZHANG lingling MI Tiezhu BAO Zhenmin XU Xiaochun Key Laboratory of Marine Environment and Ecology Ministry of Education Qingdao 266100 China Laboratory for Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China Key Laboratory of Marine Genetics and Breeding (Ocean University of China) Ministry of Education Qingdao 266003 China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China School of Applied Technology Qingdao University Qingdao 266100 China

Aureococcus anophagefferens caused brown tides for three consecutive years from 2009 to 2011 in the coastal waters of Qinhuangdao, China, with numerous, widespread ecological and economic impact on ecosystems. To understand the population dy- namics of nanoplankton during the brown tides, sequences of the V9 region of the 18S rDNA gene, used as a marker, were analyzed by Illumina sequencing to assess nanoplankton biomass, and real-time fluorescence quantitative PCR was performed to analyze spa- tial variation in the 18S rDNA copy concentrations of nanoplankton off the Qinhuangdao coast in July, 2011. The results showed that A. anophagefferens and Minutocellus polymorphus were the dominant species in the local phytoplankton community during the brown tide in July 2011. The highest 18S rDNA copy concentrations of A. anophagefferens and M. polymorphus were detected at stations SHG and FN, respectively. The central area most strongly affected by the brown tide migrated southward from 2011 to 2013. Redundancy analysis (RDA) showed that the decreasing NOx concentration might provide suitable nutrient conditions for the A. anophagefferens outbreak. During the brown tide caused by A. anophagefferens, other phytoplankton, such as diatoms, cryptophytes, chlorophytes, dinoflagellates and other flagellates, could co-occur with it. For zooplankton, due to less selective feeding behavior, Amoebozoa was the most abundant zooplankton at station SHG, while Ciliophora was the most abundant zooplankton at other sta- tions for its more selective feeding.

关键词： Aureococcus anophagefferens Minutocellus polymorphus 18S rDNA gene Illumina sequencing brown tide

Morita Equivalences Induced by Two-Sided Group Relative Hopf-Module

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Journal of Mathematical Research and Exposition 2011年第5期31卷 819-828页

作者： qiao ling GUO Qi Hui LI Rui Fang HU College of Mathematics and Information Engineering Jiaxing University Zhejiang 314001 P. R. China

Let H be a Hopf π-coalgebra over a commutative ring k with bijective antipode S, and A and B right π-H-comodulelike algebras. We show that the pair of adjoint functors （F3 = A  Bop A□ HBop -,G3 = （-）coH） between the categories A□HBopM and AMπB-H is a pair of inverse equivalences, when A is a faithfully flat π-H-Galois extension. Furthermore, the categories Moritaπ-H（A,B） and Morita □π-H（AcoH,BcoH） are equivalent, if A and B are faithfully flat π-H-Galois extensions.

关键词： Hopf group Galois extension Morita equivalence group relative Hopf-module.

Rare imaging findings of hypersensitivity pneumonitis:A case report

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World Journal of Clinical Cases 2020年第20期8卷 5057-5061页

作者： Hong-Juan Wang Xiao-Jun Chen Lin-Xia Fan qiao-ling Qi Qi-Zhang Chen Department of Respiratory Medicine Gansu Provincial People's HospitalLanzhou 730000Gansu ProvinceChina Department of Respiratory Medicine The First People's Hospital of Zhenyuan CountyLanzhou 730000Gansu ProvinceChina

BACKGROUND Hypersensitivity pneumonitis(HP)is an immune-mediated syndrome caused by allergen ***-resolution computed tomography(HRCT)of HP may show diffuse ground-glass opacity,centrilobular ground-glass nodules,areas of air-trapping,thin-walled cysts,or fibrotic *** SUMMARY A 47-year-old male patient went to the hospital complaining of cough and gradual aggravation of shortness of *** of the lung showed that multiple nodules and ground-glass high-density shadows were present in both *** addition,circular high-density shadows of various sizes were widely distributed in both lungs with relatively normal lung markings inside *** other tests did not have a positive finding that can clarify the ***,the patient underwent a lung *** pathological results showed that the lesions tended to be *** 4 mo of follow-up,the lesions in the patient's lungs were absorbed spontaneously,and the symptoms of cough and shortness of breath have *** review results suggested that the patient's disease was self-healing,which was consistent with the characteristics of *** For some patients with HP,abnormal HRCT findings,such as the lesions in the lungs,can be absorbed on their own,which is an important clue in the diagnosis of the *** diagnosis by lung biopsy is necessary when antigen exposure is unknown.

关键词： Hypersensitivity pneumonitis High-resolution computed tomography Self-healing Circular high-density images Case report

Computer Simulation of the Electronic Structures and Absorption Spectra for a KMgF3 Crystal Containing a Potassium Vacancy

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Chinese Physics Letters 2011年第3期28卷 159-162页

作者： CHENG Fang LIU Ting-Yu ZHANG Qi-Ren qiao Hai-ling ZHOU Xiu-Wen College of Science University of Shanghai for Science and TechnologyShanghai 200093

CASTEP code,based on the density functional theory(DFT)Electronic structures and absorption spectra for a perfect KMgF_(3)crystal and a KMgF_(3)crystal containing a potassium vacancy V_(K)^(-)are optimized using the CASTEP density functional theory *** calculated results indicate that the perfect KMgF_(3)crystal has no absorption in the visible energy region,however,a KMgF_(3)crystal containing V_(K)^(-)has an additional absorption band peaking at 565 nm,fitting well with the experimental result that KMgF_(3)irradiated by an electron has an additional absorption peak at 565 *** is reasonably predicted that the 565 nm absorption band is related to the existence of V_(K)^(-)in the KMgF_(3)crystal created by the electron irradiation.

关键词： absorption crystal perfect

Growth and photosynthetic responses of Fraxinus mandshurica seedlings to various light environments

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Journal of Forestry Research 2011年第3期22卷 341-346页

作者： WANG Kai ZHU Jiao-jun YAN qiao-ling YU Li-zhong SUN Yi-rong Qingyuan Experimental Station of Forest Ecology Institute of Applied Ecology Chinese Academy of Sciences Shenyang 110016 P. R. China Graduate University of Chinese Academy of Sciences Beijing 100039 P. R. China Qingyuan Experimental Station of Forest Ecology Institute of AppliedEcology Chinese Academy of Sciences Shenyang 110016 P. R. China

To determine light requirement and adaptability of Fraxinus mandshurica seedlings, the seasonal variations of photosynthetic variables were measured in 3-year-old seedlings grown under four light levels （100%, 60%, 30%, and 15% of full sunlight） with a LI-6400 portable photosynthesis system. The leaf chlorophyll content, special leaf weight, annual height and basal diameter increment of seedlings were also observed. The maximum and minimum values of net photosynthetic rate, maximum rate of carboxylation, and maximum rate of electron transport of F. mandshurica seedlings were detected with 60% and 15% of full sunlight treatments, respectively. With the decrease of light level, both light saturation point and special leaf weight significantly declined （p 0.05）, but leaf chlorophyll content significantly increased （p 0.05）. Annual height and basal diameter increments of seedlings grown under 60% of full sunlight treatment were significantly greater than those of seedlings under other treatments （p 0.05）. It was concluded that F. mandshurica seedlings can adapt to a wide range of light environments from 15% to 100% of full sunlight by adjusting light saturation point, leaf chlorophyll content and special leaf weight. According to the maximum of relative growth, 60% of full sunlight treatment is the optimum light level for the growth of 3-year-old F. mandshurica seedlings.

关键词： Fraxinus mandshurica light adaptation plasticity seasonal dynamics secondary forest

Neutralizing antibodies against SARS-CoV-2 virus after vaccination in patients with neurofibromatosis type 1

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Signal Transduction and Targeted Therapy 2023年第7期8卷 3215-3217页

作者： qiao-ling Ruan Zhi-chao Wang Cheng-jiang Wei Wei Wang Qing-luan Yang Jing Wu Yan-min Wan ling-ling Ge Wen-hong Zhang Qing-feng Li Department of Infectious Diseases National Medical Center for Infectious DiseasesShanghai Key Laboratory of Infectious Diseases and Biosafety Emergency ResponseHuashan HospitalFudan UniversityShanghaiPeople’s Republic of China Department of Plastic and Reconstructive Surgery Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghaiPeople’s Republic of China Shanghai Huashen Institute of Microbes and Infections ShanghaiPeople’s Republic of China

Dear Editor,The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has triggered a COVID-19 pandemic that has caused high morbidity and mortality worldwide.1 COVID-19 vaccines are urgently needed to protect all susceptible populations from SARS-CoV-2 *** type 1(NF1)is a hereditary dominant disease that affects approximately one in every 3000 newborns.

关键词： mortality acute respiratory

TiO_(2)-Fe_(2)TiO_(5)heterostructure hollow spheres enable high-performance lithium-sulfur batteries via interfacial engineering

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Rare Metals 2024年第8期43卷 4006-4012页

作者： Kai-Tao Liu Hong-Qiang Wang Zhuo Chen Shi-Lin Zhang Ying-Ying Song Fang Liu qiao-ling Li Jiao Li School of Materials Science and Engineering Shandong University of TechnologyZibo 255049China College of Chemistry&Environmental Science Hebei UniversityBaoding 071002China School of Chemical Engineering Faculty of SciencesEngineering and TechnologyUniversity of AdelaideAdelaide 5005Australia School of Materials Science and Engineering Hebei University of TechnologyTianjin 300130China

The shuttle effect of lithium polysulfides between sulfur cathode and lithium anode is a notorious problem in the commercial application of lithium-sulfur ***,heterostructured TiO_(2)-Fe_(2)TiO_(5)hollow spheres were ... 详细信息

关键词： lithium sulfide (5)