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Phenotypic Characterization and QTL/Gene Identification for Internode Number and Length Related Traits in Maize

American Journal of Plant Sciences 2024年第7期15卷 467-485页

作者： Jing Li Fengjuan Gu Guoqiang Wang Yingyi Zhang Xiangling Gong Wei Wei Xianchuang Zhang Lin Liu Hameed Gul Hong Duan Chaoxian Liu qianlin xiao Zhizhai Liu College of Agronomy and Biotechnology/Maize Research Institute Southwest University Chongqing China Health Commission of Suixi County Huaibei China Agriculture and Rural Bureau of Qinglong County Qinglong County China Yunyang Committee of Agriculture and Rural Affairs Chongqing China

Internode number and length are the foundation to constitute plant height, ear height and the above-ground spatial structure of maize plant. In this study, segregating populations were constructed between EHel with extremely low ear height and B73. Through the SNP-based genotyping and phenotypic characterization, 13 QTL distributed on the chromosomes (Chrs) of Chr1, Chr2, Chr5-Chr8 were detected for four traits of internode no. above ear (INa), average internode length above ear (ILaa), internode no. below ear (INb), and average internode length below ear (ILab). Phenotypic variation explained (PVE) by a single QTL ranged from 6.82% (qILab2-2) to 12.99% (qILaa5). Zm00001d016823 within the physical region of qILaa5, the major QTL for ILaa with the largest PVE was determined as the candidate through the genomic annotation and sequence alignment between EHel and B73. Product of Zm00001d016823 was annotated as a WEB family protein homogenous to At1g75720. qRT-PCR assay showed that Zm00001d016823 highly expressed within the tissue of internode, exhibiting statistically higher expression levels among internodes of IN4 to IN7 in EHel than those in B73 (P Zm00001d016823 might provide novel insight into molecular mechanism beyond phytohormones controlling internode development in maize.

关键词： Maize (Zea mays L.) Internode No. Average Internode Length Phenotypic Characterization Candidate Gene Discovery

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Discovering Candidate Chromosomal Regions Linked to Kernel Size-Related Traits via QTL Mapping and Bulked Sample Analysis in Maize

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Phyton-International Journal of Experimental Botany 2022年第7期91卷 1429-1443页

作者： Hameed Gul Mengya Qian Mohammad GArabzai Tianhui Huang Qiannan Ma Fangyu Xing Wan Cao Tingting Liu Hong Duan qianlin xiao Zhizhai Liu College of Agronomy and Biotechnology Southwest UniversityChongqing400715China Department of Agronomy Faculty of AgriculturePaktia UniversityGardiz2201Afghanistan

Kernel size-related traits,including kernel length,kernel width,and kernel thickness,are critical components in determining yield and kernel quality in maize(Zea mays L.).Dissecting the phenotypic characteristics of these traits,and discovering the candidate chromosomal regions for these traits,are of potential importance for maize yield and quality *** this study,a total of 139 F2:3 family lines derived from EHel and B73,a distinct line with extremely low ear height(EHel),was used for phenotyping and QTL mapping of three kernel sizerelated traits,including 10-kernel length(KL),10-kernel width(KWid),and 10-kernel thickness(KT).The results showed that only one QTL for KWid,i.e.,qKWid9 on Chr9,with a phenotypic variation explained(PVE)of 13.4%was detected between SNPs of AX-86298371 and AX-86298372,while no QTLs were detected for KL and KT across all 10 *** bulked groups of family lines,i.e.,Groups I to IV,were constructed with F2:3 family lines according to the phenotypic comparisons of KWid between EHel and *** these four groups,Group I possessed a significantly lower KWid than EHel(P=0.0455),Group II was similar to EHel(P=0.34),while both Group III and Group IV were statistically higher than EHel(P<0.05).Besides,except Group IV exhibited a similar KWid to B73(P=0.11),KWid of Groups I to III were statistically lower than B73(P<0.00).By comparing the bulked genotypes of the four groups to EHel and B73,a stable chromosomal region on Chr9 between SNPs of AX-86298372 to AX-86263154,entirely covered by qKWid9,was identified to link KWid with the positive allele of increasing phenotypic effect to KWid from B73,similar to that of qKWid9.A large amount of enzyme activity and macromolecule binding-related genes were annotated within this chromosomal region,suggesting qKWid9 as a potential QTL for KWid in maize.

关键词： Maize(Zea mays L.) kernel size-related traits QTL mapping bulked sample analysis(BSA)

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Transcription factor ZmNAC126 plays an important role in transcriptional regulation of maize starch synthesis-related genes

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The Crop Journal 2021年第1期9卷 192-203页

作者： qianlin xiao Yayun Wang Hui Li Chunxia Zhang Bin Wei Yongbin Wang Huanhuan Huang Yangping Li Guowu Yu Hanmei Liu Junjie Zhang Yinghong Liu Yufeng Hu Yubi Huang State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130SichuanChina College of Agronomy and Biotechnology Southwest UniversityChongqing 400716China College of Agronomy Sichuan Agricultural UniversityChengdu 611130SichuanChina College of Life Science Sichuan Agricultural UniversityYa'an 625014SichuanChina Maize Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Sichuan Seed Station Chengdu 610041SichuanChina

Maize(Zea mays L.)is one of the most important food crops in the world,and starch is the main component of its *** regulation plays a vital role in starch ***,it is not well understood in *** report the identification of the transcription factor ZmNAC126 and its role in regulation of starch synthesis in *** expression of ZmNAC126 was higher in maize endosperm and kernels than in roots or ***126 shared a similar expression pattern with starch synthesis genes during seed development,and its expression pattern was also consistent with the accumulation of ***126 is a typical transcription factor with a transactivation domain between positions 201 and 227 of the amino acid sequence,is located in the nucleus,and binds to CACG repeats in *** one-hybrid assay revealed that ZmNAC126 bound the promoters of ZmGBSSI,ZmSSIIa,ZmSSIV,ZmISA1,and *** overexpression of ZmNAC126 in maize endosperm increased the activities of promoters pZmSh2,pZmBt2,pZmGBSSI,pZmSSIIIa,and pZmBT1 but inhibited the activities of pZmISA1 and ***126 thus acts in starch synthesis by transcriptionally regulating targeted starch synthesis-related genes in maize kernels.

关键词： Maize Starch synthesis ZmNAC126 Co-expression Transcription regulation

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Maize (Zea maysL.) Productivity in Response to Nitrogen Management in Pakistan

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American Journal of Plant Sciences 2021年第8期12卷 1173-1179页

作者： Hameed Gul Saeedur Rahman Ali Shahzad Shareef Gul Mengya Qian qianlin xiao Zhizhai Liu College of Agronomy and Biotechnology Southwest University Chongqing China Department of Agronomy The University of Agriculture Peshawar Pakistan Department of Agronomy University of Swabi Anbar (KPK) Pakistan

Nitrogen (N) plays a vital role in the productivity of maize (Zea mays L). To investigate the fertilizer effects of N on the yield and growth of maize hybrid variety (Gorilla), the experiment was carried out at the research farm of University of Swabi, Pakistan, during summer 2017-18. Four levels of N (Urea, Urea + Farm Yard Manure (FYM), Urea + Compost, Urea + Poultry Manure (PM)) were set in the present study. Randomized complete block design (RCBD) was used with split-plot arrangement with N administering to main plot. Results showed that yield and other traits, i.e. plant height, ear length, ear weight, kernel yield, kernels ear-1 and harvest index (HI) were significantly affected by Nitrogen. In current study, the maximum performances of plant height (231.46 cm), ear length (12.17 cm), kernels ear-1 (434.83), kernel yield (2095.7 kg·ha-1), total kernel weight (350.75 kg·ha-1), biomass yield (4015.3 kg·ha-1) and HI (37.31) were recorded under the treatment of UREA + PM, and followed by UREA + FYM. Besides, the applications of organic manure in combination with nitrogen significantly increased yield and its components. Application of 50% of N and 50% of poultry manure produced higher performance for the traits of plant height, ear length, kernels ear-1, total kernels weight ear-1, kernel yield, and biomass yield.

关键词： Maize (Zea mays L.) Nitrogen Yield Yield Components Pakistan

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原料组成对多微孔方镁石-镁铁铝复合尖晶石陶瓷显微结构与性能的影响

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硅酸盐通报 2024年第7期43卷 2672-2679页

作者：陈茜琳鄢文王晓李亚伟武汉科技大学省部共建耐火材料与冶金国家重点实验室武汉430081 武汉科技大学钢铁工业耐火材料新技术教育部国际合作联合实验室武汉430081 青海大学化工学院西宁810016

利用原位分解合成法,以轻烧MgO、Fe2O3和Al(OH)3为原料,成功制备了多微孔方镁石-镁铁铝复合尖晶石陶瓷,并研究原料组成对其显微结构与性能的影响。研究结果表明:当理论镁铁铝复合尖晶石含量为0%(质量分数,下同)时,试样中颈部连接形成较... 详细信息

利用原位分解合成法,以轻烧MgO、Fe2O3和Al(OH)3为原料,成功制备了多微孔方镁石-镁铁铝复合尖晶石陶瓷,并研究原料组成对其显微结构与性能的影响。研究结果表明:当理论镁铁铝复合尖晶石含量为0%(质量分数,下同)时,试样中颈部连接形成较少,耐压强度较低;当理论镁铁铝复合尖晶石含量为4%~16%时,试样中生成的液相量增多,物质运输速率加大,形成的颈部连接较多,耐压强度较高;当理论镁铁铝复合尖晶石含量为24%时,试样中镁铁铝复合尖晶石生成带来的体积膨胀增多,增大颗粒间距,颈部连接的形成减少,试样的耐压强度减小。同时,当理论镁铁铝复合尖晶石含量为12%~16%时,多微孔方镁石-镁铁铝复合尖晶石陶瓷具有较高的显气孔率(22.3%~24.6%)、较低的体积密度(2.75~2.80 g/cm^(3))和较高的耐压强度(100.6~123.1 MPa)。

关键词：多微孔陶瓷方镁石-镁铁铝复合尖晶石原料组成原位分解合成法显微结构耐压强度

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融合均值榜样的反向互学习水母搜索算法

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河南师范大学学报（自然科学版） 2024年第4期52卷 111-119,I0015,I0016页

作者：段艳明肖辉辉谭黔林河池学院大数据与计算机学院广西河池546300 河池学院广西蚕桑生态学与智能化技术应用重点实验室广西河池546300

为解决水母搜索算法(jellyfish search algorithm,JS)的洋流运动缺乏多样性、群内运动缺乏引导性、种群间信息无交流,造成搜索速度慢、稳定性差及易早熟的问题,构建了一种融合均值榜样的反向互学习水母搜索算法(oppositional-mutual lea... 详细信息

为解决水母搜索算法(jellyfish search algorithm,JS)的洋流运动缺乏多样性、群内运动缺乏引导性、种群间信息无交流,造成搜索速度慢、稳定性差及易早熟的问题,构建了一种融合均值榜样的反向互学习水母搜索算法(oppositional-mutual learning jellyfish search algorithm based on mean-value example,OMLJS).首先在水母跟随洋流运动(全局搜索)部分,利用前两代水母的平均位置代替只考虑上一代水母的平均位置来引导水母个体的位置更新,提高算法的全局搜索能力;其次在水母的群内主动运动(局部搜索)部分,利用最优个体代替随机个体来引导水母进行更有效的搜索,加快算法的收敛速度;然后在水母进入下一次迭代前增加对水母种群进行动态反向互学习步骤,增加种群多样性及增强种群间的信息交流,达到互补另外两个策略,提高算法的整体优化性能.选用12个经典的基准测试优化函数,将OMLJS与5个对比算法从解的平均值、最优值及方差进行对比分析,并用于求解最小生成树问题,OMLJS能够更快地找到最小生成树.实验结果表明,OMLJS的收敛速度、求解精度明显提高.

关键词：水母搜索算法均值榜样学习反向互学习时间控制机制最小生成树问题

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对教师“转会”制的理性诉求

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科技广场 2005年第11期 80-81页

作者：金绍荣肖前玲西南大学教育学院重庆400715

教师“转会”制是近几年来,一些地方教育主管部门在探索管理教师流动问题时,推出的一种教师管理新举措,它是以引入市场力量来管理教师队伍的一种尝试。它的产生以社会分层流动为动因;以弱势补偿机制为伦理支持;以教师聘任制为保障;以政... 详细信息

教师“转会”制是近几年来,一些地方教育主管部门在探索管理教师流动问题时,推出的一种教师管理新举措,它是以引入市场力量来管理教师队伍的一种尝试。它的产生以社会分层流动为动因;以弱势补偿机制为伦理支持;以教师聘任制为保障;以政府宏观调空为导向。在理论和实践中,我们还要更加对其理性诉求,使其能够健康发展。

关键词：教师 “转会”制理性诉求

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机器人辅助腹腔镜与传统腹腔镜关于全子宫切除术在不同体质指数患者中的对比研究

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重庆医科大学学报 2024年第1期49卷 70-74页

作者：朱梦秋易倩琳武加林陈万丽肖琳重庆医科大学附属第一医院妇科重庆400016

目的:比较机器人辅助腹腔镜与传统腹腔镜关于全子宫切除术在不同体质指数患者中的安全性及可行性。方法:回顾性收集2016年2月至2021年4月于重庆医科大学附属第一医院接受了机器人辅助腹腔镜全子宫切除术(n=416)和传统腹腔镜全子宫切除术... 详细信息

目的:比较机器人辅助腹腔镜与传统腹腔镜关于全子宫切除术在不同体质指数患者中的安全性及可行性。方法:回顾性收集2016年2月至2021年4月于重庆医科大学附属第一医院接受了机器人辅助腹腔镜全子宫切除术(n=416)和传统腹腔镜全子宫切除术(n=725)患者的病例资料。按照不同等级体质指数(body mass index,BMI)对患者进行分层(非超重、超重和肥胖),比较2组中各BMI亚组间患者的围术期情况、术中及术后并发症。结果:机器人组中超重亚组的手术时间短于传统腹腔镜组[(125.37±50.92) min vs.(135.68±44.55) min],机器人组中3个BMI亚组的术中出血量均少于传统腹腔镜组[(60.46±45.20) mL vs.(92.88±105.13) mL、(72.01±62.03) mL vs.(115.02±123.38) mL及(65.59±37.92) mL vs.(125.16±96.07) mL],机器人组中超重亚组和肥胖亚组的首次排气时间均短于传统腹腔镜组[(2.04±0.83) d vs.(2.19±0.63) d和(1.97±0.54) d vs.(2.19±0.54) d],差异均有统计学意义(P0.05)。结论:超重和肥胖患者的全子宫切除术,机器人辅助腹腔镜相较于传统腹腔镜在围术期降低并发症等方面有一定优势。

关键词：机器人辅助腹腔镜传统腹腔镜全子宫切除术体质指数围术期

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Preliminary Phenotypic and SNP-Based Molecular Characterization of Maize (Zea maysL.)-Mexicana (Zea maysSSP. Mexicana) Introgression Lines under Inbred Background of 48-2

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American Journal of Plant Sciences 2021年第7期12卷 1073-1089页

作者： Abdoul Raouf Sayadi Maazou Ju Qiu Ling Ai Mohammad G. Arabzai Mengya Qian Hameed Gul Xuemei Du Fangyu Xing Jiarui Yan Keyi Zhang Ziqi Zhang Qiannan Ma Tianhui Huang Hong Duan qianlin xiao Zhizhai Liu College of Agronomy and Biotechnology Southwest University Chongqing China Pan African University Life and Earth Sciences Institute (Including Health and Agriculture) University of Ibadan Ibadan Nigeria International Institute of Tropical Agriculture (IITA) Ibadan Nigeria Paktia University Gardiz Afghanistan

Wild relatives possess potential genetic diversity for maize (Zea mays L.) improvement. Characterization of maize-mexicana introgression lines (ILs) is of great value to diversify the genetic base and improve the maize germplasm. Four maize-mexicana IL generations, i.e. BC1, BC2, BC3, and RIL, were constructed under the elite inbred background of 48-2, elite inbred line that is widely used in maize breeding in Southwestern China, and were phenotyped in different years and genotyped with 56110 SNPs. The results indicated that 48-2 had higher phenotypic performances than all the characterized ILs on most of the agronomic traits. Compared with other ILs, BC2 individuals exhibited more similar performance to 48-2 on most traits and possessed the highest kernel ratio (66.5%). Population structure and principal component analysis indicated that BC3 individuals gathered closer to 48-2 and exhibited the lowest mexicana-introgression frequency (0.50%), while BC2 (29.06%) and RIL (18.52%) showed higher introgression frequency. The high level of genetic diversity observed in the maize-mexicana ILs demonstrated that Z. mays ssp. mexicana can serve as a potential source for the enri

关键词： Maize (Zea mays L.) Mexicana (Zea mays SSP. Mexicana) Introgression Lines Phenotypic Characterization Molecular Characterization

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高粱淀粉合成酶新基因SSV的鉴定与特征分析

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核农学报 2016年第1期30卷 1-10页

作者：于桂玲肖前林魏彬杨进王丹刘汉梅四川农业大学生命科学学院四川雅安625014 四川农业大学农学院四川成都611130 濮阳市农业科学院河南濮阳457000

淀粉合成酶(SS)是植物淀粉生物合成过程中的关键酶之一,目前已鉴定并揭示功能的淀粉合成酶有5个亚型。为利用丰富的基因组数据鉴定高粱基因组里的淀粉合成酶新亚型基因,本文利用生物信息学和分子生物学方法,首次鉴定并分离了编码高粱淀... 详细信息

淀粉合成酶(SS)是植物淀粉生物合成过程中的关键酶之一,目前已鉴定并揭示功能的淀粉合成酶有5个亚型。为利用丰富的基因组数据鉴定高粱基因组里的淀粉合成酶新亚型基因,本文利用生物信息学和分子生物学方法,首次鉴定并分离了编码高粱淀粉合成酶的新亚型基因Sb SSV,并对此基因的内含子-外显子结构、表达特性等进行了分析。结果显示,该基因的全长ORF为2 097 bp,其外显子数量、长度及内含子的位置分布等与玉米和水稻的直系同源基因基本一致,推导的蛋白具有细菌糖原合成酶和植物淀粉合成酶特有的糖基催化和糖基转移保守结构域。系统进化分析表明,Sb SSV与已知的SSIV亚型亲缘关系最近,推测本研究鉴定的Sb SSV基因编码高粱淀粉合成酶新亚型。定量PCR分析表达特性结果显示Sb SSV主要在高粱叶片中表达,其表达受光诱导,具有昼夜节律特性。研究结果可为深入揭示和完善植物淀粉合成代谢机制,以及改良植物产量和品质提供理论基础。

关键词：高粱淀粉淀粉合成酶V 新基因鉴定

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