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Advances and challenges towards the study of RNA-RNA interactions in a transcriptome-wide scale

中国生物化学与分子生物学会2019年全国学术会议暨学会成立四十周年

作者： Huili Wang Kequan Lin Lin Zhu Shaojun zhang Le Li Yilie Liao Baichao zhang Ming Yang Xinde Liu Lu Li Shasha Li Haitao Li Peng Jiang qiangfeng cliff zhang Dong Wang School of Medicine Tsinghua University School of Life Sciences Tsinghua University Center of Synthetic & Systems Biology Tsinghua University National Collaborative Innovation Center for Biotherapy Tsinghua University School of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine

Aerobic glycolysis, also known as the Warburg effect, is a hallmark of cancer and essential for malignancies, but its regulation and modulation in cancer cells remain poorly understood. Here, by large-scale functional screening, we identified a tumorassociated and broadly expressed oncogenic long noncoding RNA TURBOR, which is highly expressed in multiple types of human cancers. Notably, knocking down TURBOR significantly inhibits the proliferation of multiple types of cancer cells and reduces tumor growth in vivo. Mechanistically, TURBOR directly binds to lactate dehydrogenase A(LDHA), an essential glycolysis-associated enzyme, and enhances its enzymatic activity, thereby promoting glycolysis. Clinically, high expression of TURBOR is significantly associated with poor prognosis in many types of human cancers. Together, our results identified a new long noncoding RNA(lncRNA) that modulated a cancer-specific regulation mechanism for the Warburg effect and suggested a potential target against one of cancer’s vulnerabilities for developing broad-acting anti-cancer therapies.

关键词： Long noncoding RNA Warburg effect Tumor proliferation

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Frontiers of Electrical and Electronic Engineering in China 2018年第3期6卷 239-252页

作者： Jing Gongt Yanyan Jut Di Shao qiangfeng cliff zhang MOE Key Laboratory of Bioinformatics Beijing Advanced Innovation Center for Structural BiologyCenter for Synthetic and Systems BiologyTsinghua-Peking Joint Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China

RNA molecules play crucial roles in various biological processes. Their regulation and function are mediated by interacting with other molecules. Among them RNA-RNA interactions （RRIs） are important in many basic cellular activities including transcription, RNA processing, localization, and translation. However, we just start to unveil the complexity of the knowledge and underlying mechanisms of RRIs. Results： In this review, we will summarize approaches for RRI identifications, including both conventional, focused biophysical and biochemical methods and recently developed large scale sequencing-based techniques. We will also discuss discoveries per RRI type revealed by using these technologies, as well as challenges towards a systematic and functional understanding of RRIs. Conclusions： The development of sequencing-based techniques has revolutionized the study of RRIs. Applying these techniques in multiple organisms has identified thousands of RRls, many of which could potentially regulate multiple aspects of gene expression. However, despite the great breakthrough, the RNA-RNA interactome of any species remains far from complete due to intrinsic complex nature of RRI and limitations in current techniques. More efficient experimental methods and computational framework are needed to obtain the full image of RRI networks, and their possible regulatory roles in biology and medicine.

关键词： RNA-RNA interactions PARIS SPLASH LIGR-seq next generation sequencing

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人源Dicer蛋白及其结合pre-miRNA底物复合物的冷冻电镜结构研究

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科学新闻 2019年第2期 47-47页

作者：刘忠民王家 Hang Cheng Xin Ke Lei Sun qiangfeng cliff zhang 王宏伟清华大学生命科学学院

文章简介人源Dicer蛋白属于RNase III家族成员,由多个结构域组成。人源Dicer蛋白能够识别结合内源性和外源性的小RNA前体,并加工生成长度约为22nt的小RNA。小RNA通过其序列特异性引导沉默复合体作

关键词： Dicer 冷冻电镜结构 pre-miRNA 复合物

Efficient Synthesis of PbTe Nanoparticle Networks

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Nano Research 2010年第10期3卷 685-693页

作者： qiangfeng Xiao Ding Weng Zhenglong Yang Javier Garay Minjuan zhang Yunfeng Lu Chemical and Biomolecular Engineering Department University of CaliforniaLos AngelesCA 90095USA Mechanical Engineering Department University of CaliforniaRiversideCA 92521USA Materials Research Department Toyota Motor Engineering and Manufacturing North AmericaInc.1555 Woodridge Ave.Ann ArborMI 48105USA

The synthesis of semiconductor nanocrystalline networks using weak capping ligands in aqueous media has been ***,includingβ-cyclodextrin,D-(+)-glucose,D-glucosamine,lactobionic acid,sucrose,and starch were chosen as weak ligands to facilitate the formation of PbTe nanoparticle *** nanoparticle size,ranging from 5 nm to 30 nm,can be tuned by manipulating the temperature and *** a similar strategy,more complicated nanostructures including carbohydrate spheres@PbTe core-shell structures and Te@carbohydrate@PbTe multilayered submicron cables have been *** is a general approach which can be easily extended to the fabrication of other semiconductor networks,including PbSe and Bi2Te3 using carbohydrates and ethylenediaminetetraacetic acid(EDTA),respectively,as ligands.

关键词： Lead telluride nanoparticle network core shell structures thermoelectric carbohydrate

High performance octahedral PtNi/C catalysts investigated from rotating disk electrode to membrane electrode assembly

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Nano Research 2019年第2期12卷 281-287页

作者： Bing Li Jue Wang Xin Gao Congwei Qin Daijun Yang Hong Lv qiangfeng Xiao Cunman zhang School of Automotive Studies & Clean Energy Automotive Engineering Center Tongji University (Jiading Campus) 4800 Caoan Road Shanghai 201804 China Department of Energy Technology Aalborg University Pon toppidanstraede 101 Aalborg 9220 Denmark

Octahedral PtNi/C catalysts have demonstrated superior catalytic performance in oxyge n reduction reacti on (ORR) over commercial Pt/C with rotating disk electrode (RDE). However, it is not trivial to translate such promising results to real-world membrane-electrode assembly (MEA). In this work, we have synthesized octahedral PtNi/C catalysts using poly(diallyldimethylammonium chloride)(PDDA) as a capping age nt and in vestigated their performance from RDE to MEA. In RDE, mass activity and specific activity of the optimized octahedral PtNi/C catalyst for oxygen reduction reaction (ORR) are nearly 19 and 28 times high of the state-of-the-art commercial Pt/C, respectively. At MEA level, the octahedral PtNi/C catalyst exhibits excelle nt power generation performa nee and durability paired with commercial Pt/C ano de. Its cell voltage at 1,000mA·cm^-2 reaches 0.712 V, and maximum power density is 881.6 mW·cm^-2 and its performance attenuation is also less, around 11.8% and 7% under galvanostatic condition of 1,000 mA·cm^-2 for 100 h. Such results are investiaged by thermodynamic analysis and fundametal performance modeling, which indicate the single cell performance can be further improved by reducing the size of PtNi/C catalyst agglomerates. Such encouraging results have demonstrated the feasibility to convey the superior performance of octahedral PtNi/C from RDE to MEA.

关键词： proton exchange membrane fuel cell (PEMFC) octahedral PtNi/C oxygen reduction reaction (ORR) durability membrane electrode assembly (MEA)

Regulating composition and structure of coal-based graphene and its electrochemical characteristics

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Clean Energy 2024年第5期8卷 265-276页

作者： Wenfeng Liu Jun Li Yating zhang Keke Li Zhen Liu cliff Yi Guo Jingjing Wang Linlin Wei China Energy Investment Corporation No.22XiBinhe RoadAndingmenDongcheng DistrictBeijing100011China National Institute of Clean-and-Low-Carbon Energy China Energy Investment CorporationBinhe Road No.9Future Science&Technology CityChangping DistrictBeijing102211China of Chemistry and Chemical Engineering Xi’an University of Science and TechnologyNo.58Yanta RoadXi'anShaanxi Province710054China

Coal,a carbon-rich mineral with plentiful reserves,serves not only as a fuel but also as a raw material,presenting lower pollution emissions in the latter *** a materials chemistry standpoint,coal is a viable raw material for graphene *** study develops a promising and sustainable method to convert coal into graphene,leveraging its unique macromolecular aromatic struc-ture and high carbon *** investigation includes an analysis of the lateral size,morphology,and chemical composition of coal-derived graphene using techniques such as X-ray diffraction,Raman spectroscopy,X-ray photoelectron spectroscopy,and op-tical *** confirm that coal can effectively replace natural graphite flakes in graphene production,with the derived graphene featuring three to six exfoliated layers and an oxygen content below 5.5%.While the graphene from coal shares a similar morphology to that derived from graphite,it exhibits more structural ***,the macroscopic size of the coal does not influence the microscopic composition and structure of the ***,the thermal reduction method for oxidized graphene proves more effective at repairing structural defects than chemical *** coal-derived graphene as a supercapacitor electrode demonstrates excellent cycling stability and ultra-high capacitance storage *** H-CG-325 shows the highest dis-charge area-specific capacitance across various current *** an increased current density of 10 A/g,the H-CG-325 maintains 80.6%of its initial capacitance of 79 F/g observed at 1 A/*** tests reveal that coal-based graphene holds significant potential as a supercapacitor material,indicating promising applications in energy storage and conversion.

关键词： coal graphene graphene oxide chemical reduction high-temperature reduction

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加拿大身体素养测评演变的解析与启示

体育科学 2020年第8期40卷 41-48页

作者：张强峰湖南师范大学湖南长沙410012

如何测评身体素养是一个难题,但加拿大身体素养测评(CAPL)颇有建树。通过文献资料调研、逻辑推理、对比分析等方法,文章就CAPL出台的历史背景与演变沿革进行解析,探究CAPL演变的主要原因,剖析CAPL演变历程中身体素养测评的趋向与不足,... 详细信息

如何测评身体素养是一个难题,但加拿大身体素养测评(CAPL)颇有建树。通过文献资料调研、逻辑推理、对比分析等方法,文章就CAPL出台的历史背景与演变沿革进行解析,探究CAPL演变的主要原因,剖析CAPL演变历程中身体素养测评的趋向与不足,提出对我国身体素养测评的启示。CAPL演变的主要原因为样本来源有待扩充,维度赋分尚需更正,标准评价尚待细化。CAPL演变体现出四维度紧密衔接、多学科交叉融合、人性化实施趋向,但存在测量与身体素养内涵有出入、评价并未摆脱传统线性评价窠臼、与学校体育结合有待研究等问题。未来我国身体素养测评在数据收集上应长期纵向而不是瞬时横向,测评对象上应测评人和物而不是单纯测评人,评价功能上应注重整体评价与个体反馈而不是群体甄别。

关键词：加拿大身体素养测量评价

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Based on Fluent Numerical Calculation of Refractive Index on Rocket Engine Nozzle Plume

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Optics and Photonics Journal 2013年第2期3卷 90-93页

作者： qiangfeng Huang Xiong Wan Zhimin zhang Huaming zhang Hengyang Shen Tingting Du 1Key Laboratory of Nondestructive Testing (Ministry of Education) Nanchang Hangkong University Nanchang Jiangxi China 2Key Laboratory of Spatial Active Opto-electronic Techniques Shanghai Institute of Technical Physics Chinese Academy of Sciences Shanghai China Key Laboratory of Nondestructive Testing (Ministry of Education) Nanchang Hangkong University Nanchang Jiangxi China

Numerical 2D simulation and research on internal flow field and external flow field of rocket motor nozzle using FLUENT software. Analyze the flow condition of internal flow field and external flow field, and according to add in the amount of the different gas components, obtain the clear distribution of contour of density flow field, pressure flow field and various material components and so on. Simulation results agree with the results observed from the test on the ground, and provide reference for solid rocket motor development.

关键词： Rocket Engine Nozzle Nozzle Plume Fluent Numerical Simulation

Oxygen-doped carbon host with enhanced bonding and electron attraction abilities for efficient and stable SnO_2/carbon composite battery anode

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Science China Materials 2018年第8期61卷 1067-1077页

作者： Zhen Geng Bing Li Hezhi Liu Hong Lv qiangfeng Xiao Yongjun Ji Cunman zhang Clean Energy Automotive Engineering Center Tongji UniversityShanghai 201804China Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China State Key Laboratory of Multiphase Complex Systems Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China General Motors Research and Development Center WarrenMI 48090USA

The coupling between electrochemically active material and conductive matrix is vitally important for high efficiency lithium ion batteries （LIBs）. By introducing oxygen groups into the nanoporous carbon framework, we accom- plish sustainably enhanced electrochemical performance for a SnO2/carbon LIB. 2-5 nm SnO2 nanoparticles are hydro- thermally grown in different nanoporous carbon frameworks, which are pristine, nitrogen- or oxygen-doped carbons. Compared with pristine and nitrogen-doped carbon hosts, the SnO2/oxygen-doped activated carbon （OAC） composite ex- hibits a higher discharge capacity of 1,122mAhg^-1 at 500 mA g^-1 after 320 cycles operation and a larger lithium storage capacity up to 680 mAhg-I at a high rate of 2,000 mA g^-1. The exceptional electrochemical performance originated from the oxygen groups, which could act as Lewis acid sites to attract electrons effectively from Sn during the charge process, thus accelerating reversible conversion of Sn to SnO2. Meanwhile, SnO2 nanoparticles are effectively bonded with carbon through such oxygen groups, thus preventing the electrochemical sintering and maintaining the cycling stability of the SnO2/OAC composite anode. The high electrochemical performance, low biomass cost, and facile preparation renders the SnO2/OAC composites a promising candidate for anode materials.

关键词： tin oxide nanoporous carbon functional groups anode materials lithium-ion batteries

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