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2010中国青年遗传学家论坛

作者： qian liu and chi-kuang wen~* national key laboratory of plant molecular genetics,institute of plant physiology and ecology,shanghai institutes for biological sciences,chinese academy of sciences,300 fenglin rd,shanghai 2.0032,china

Ethylene responses are modulated by a family of five ethylene receptor members in *** five receptors are genetically redundant while not functionally exchangeable;how the five receptor members may act coordinately remains *** addressed this question by examining the signal output of transmitter-on ETR1 or ERS1 in the absence of other wild-type receptors or in the presence of a wild-type receptor *** to a singling active state by silver or 1-MCP, ETR1 alone was able to reverse ethylene growth inhibition to a great extent while ERS1 was ***-1, ersl-1,and erslC65Y are transmitter-on receptor ***-1 substantially repressed ethylene responses in quintuple receptor knockout mutants while ersl-1 did *** inhibition of ethylene responses by ersl-1 is mainly dependent on ETR1 or ***4 had a greater role than ETR1 in erslC65Y *** lack of both ETR1 and EIN4 abolished the ability of erslC65Y in the repression of ethylene,*** show that the wild-type receptors are not dispensable to the signal output of a transmitter-on ethylene receptor,implying collaborative receptor *** collaborations may facilitate a plant to make corresponding responses to a wide range of ethylene *** redundancy and functional divergence of the five receptor members are essential to the maintenance of collaborative receptor signaling.

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The differential reversion of ethylene response by ETRl and ERS1 implies functional significance of ethylene receptor collaboration

The differential reversion of ethylene response by ETRl and ...

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2011全国植物生物学研讨会

作者： qian liu and chi-kuang wen~* national key laboratory of plant molecular genetics,institute of plant physiology and ecology, shanghai institutes for biological sciences,chinese academy of sciences,300 fenglin rd,shanghai 2.0032,china

In Arabidopsis,the ethylene signal is mediated by a family of five receptor members while little is known about the nature of the receptor signal and how the five receptor members may act *** we show that ETR1,but not ERS 1,was able to reverse ethylene growth inhibitions to a great extent upon 1-MCP,an ethylene antagonist,treatment in the absence of other ***-1,ers 1-1,and ers 1 are ethylene-insensitive ***-1 substantially repressed various ethylene responses independent of other receptor *** inhibition of ethylene response

关键词： Ethylene etr1-1 ers1-1 ers1C65Y receptor collaborations

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A Study of Transmitter-on ETR1 and ERS1 Signaling Implies Differential Collaborations of Arabidopsis Ethylene Receptors

A Study of Transmitter-on ETR1 and ERS1 Signaling Implies Di...

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植物分子生物学与现代农业——2010全国植物生物学研讨...

作者： qian liu and chi-kuang wen~* national key laboratory of plant molecular genetics,institute of plant physiology and ecology, shanghai institutes for biological sciences,chinese academy of sciences,shanghai 2.0032,china

Ethylene responses are modulated by a family of five ethylene receptor members in *** the five receptor members may function individually or collaboratively remains *** addressed this question by examining if transmit...

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RTE1 Is a Golgi-Associated and ETR1-Dependent Negative Regulator of Ethylene Responses

RTE1 Is a Golgi-Associated and ETR1-Dependent Negative Regul...

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2007年全国植物生长物质研讨会

作者： Xin Zhou qian liu Fang Xie chi-kuang wen~* national key laboratory of plant molecular genetics institute of plant physiology and ecology shanghai institutes for biological sciences chinese academy of sciences shanghai 2.0032 china

＜正＞Arabidopsis（Arabidopsis thaliana）RTE1 encodes a membrane protein and negatively regulates ethylene *** and transformation studies suggest that the function of the wild-type RTE1 is primarily dependent on ETR1 ...

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Identification and Characterization of glnA Promoter and its Corresponding Trans-regulatory Protein GlnR in the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32

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Acta Biochimica et Biophysica Sinica 2006年第12期38卷 831-843页

作者： Hao YU~(1&) wen-Tao PENG~1 Yang liu~1 Ting WU~2 Yu-Feng YAO~1 Ming-Xue CUI~1 Wei-Hong JIANG~1 Guo-Ping ZHAO~(1,3,4*) 1 laboratory of molecular Microbiology,institute of plant physiology and ecology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 2.0032,china 2 Department of Medicinal Chemistry,School of Pharmacy,Fudan University,shanghai 2.0032,china 3 State key laboratory of Genetic Engineering & Department of Microbiology,School of Life Science,Fudan University,shanghai 200433,china 4 laboratory of Disease and Health Genomics,chinese national Human Genome Center at shanghai,shanghai 201203,china laboratory of molecular Microbiology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China Department of Medicinal Chemistry School of Pharmacy Fudan University Shanghai 200032 China State key laboratory of Genetic Engineering & Department of Microbiology School of Life Science Fudan University Shanghai 200433 China laboratory of Disease and Health Genomics Chinese National Human Genome Center at Shanghai Shanghai 201203 China

The genetic requirements for the transcription of glnA,encoding the major glutaminesynthetase in a rifamycin SV-producing Amycolatopsis mediterranei strain,U32,were *** experiments showed that the promoter of U32 glnA （pglnA） was likely to have two transcriptioninitiation sites:P1and P2,located 157 and 45 nucleotides （nt） upstream of the translational start codon,*** mobility shift and DNase I footprinting analyses revealed a 30 bp cis-element locatedat 45 to 75 nt downstream of P1,or 38 to 68 nt upstream of *** sequence of the cis-element displayedhigh similarity to the corresponding regions of pglnA from Streptomyces coelicolor and *** as a reporter gene,the expression levels of U32 pglnA and its deletion derivatives under differentnitrogen-source conditions were analyzed by detecting the catechol dioxygenase activities in ***54,*** J508 and *** FS10 （glnR mutant）.These in vivo studies showed that theactivation of U32 pglnA in *** required GlnR,and its binding to the U32 pglnA was furtherconfirmed by the gel mobility shift *** and heterologous expression of the U32 glnR allowed usto detect the in vitro interaction between the U32 GlnR and the corresponding pglnA *** shown by in vivo glnR inactivation and complementation indicated that GlnR is essential for theactive transcription of glnA in U32.

关键词： glnA promoter cis-element glnR Amycolatopsis mediterranei

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Negative Regulation of Receptor Signaling by ERS1

Negative Regulation of Receptor Signaling by ERS1

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上海市植物生理学会成立三十周年庆祝活动暨第三届上海市植物生理学青年学术研讨会

作者： qian liu, Chan Xu, and chikuang wen* national laboratory of plant molecular genetics, institute of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 2.0032

Previous studies revealed that the five Arabidopsis ethylene receptor genes have partially redundant functions and negatively regulate ethylene response. Genetic redundancy limits further

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Polar auxin transport in rice leaf development

Polar auxin transport in rice leaf development

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2006中国植物细胞发育与分子生物学学术研讨会

作者： qian Xu, Zhi-Hong Xu, Hong-Wei Xue national key laboratory of plant molecular genetics, institute of plant physiology & ecology, shanghai institutes for biological sciences, chinese academy of sciences, 300 fenglin Road, 2.0032 shanghai, P.R. china.

＜正＞The PAT （polar auxin transport） is required for the leaf pattern formation and vascular differentiation in dicots, however, fewer studies have been performed in monocots. To investigate the effects of PAT on leaf development in monocots, PAT inhibitors were used to treat the rice seedlings. Observations showed that leaves of treated seedlings were twisted irregularly, and in addition, the vascular continuity at the tip and base of leaf blade was defected. The xylogenesis of vascular elements were enhanced, and moter cells became larger, mesophyllous cells and the parenchyma cells around vascular bundle showed massive proliferation.

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OXP1,a Vulnerable Protein to Oxidative Modification,Plays a Role in Starch Accumulation and Development in Arabidopsis

OXP1,a Vulnerable Protein to Oxidative Modification,Plays a ...

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植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Xun-Liang liu~1,Hai-Dong Yu~1,Yuan Guan,Fang-Qing Guo~* The national key laboratory of plant molecular genetics,institute of plant physiology & ecology, shanghai institutes for biological sciences,chinese academy of sciences,300 fenglin Road, shanghai2.0032,china

Carbonylation,an oxidative modification of proteins,is most commonly occurring during abiotic *** is thought to be an irreversible protein damage process and can be used as an overall protein oxidation *** modification usually leads to the loss of function of target proteins,which undergo proteolytic degradation or form

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Photosynthetic Characteristics of Two New Chlorophyll B-less Rice Mutants

Photosynthetic Characteristics of Two New Chlorophyll B-less...

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中国植物生理学会全国学术年会暨成立40周年庆祝大会

作者： Zhi-Fang LIN, Chang-Lian PENG, Gui-Zhu LIN, Zhi-Ying OU, Cheng-Wei YANG and Jing-liu ZHANG ( South china institute of Botany, chinese academy of sciences, Guangzhou 510650, P. R. china) ( national key laboratory of plant molecular genetics, institute of plant physiology & ecology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 2.0032, P.R.china)

Two yellow rice mutants VG28-1 and VG30-5 were obtained during the tissue culture process ofposterity from a rice plant(cv. Zhonghua No.11,Japanica) with inserted maize Ds transposon element. Theabsorption spectra and pigment composition showed that two mutants had no chlorophyll b and lowerchlorophyll αcontent in comparison to the wild type. Net photosynthetic rate, J, q, φPSII, fraction of P andleaf area were lower but Fv/Fm and apparent quantum yield remained at similar levels as in the wild type *** cycle pool size(V+A+Z)on a chlorophyll basis and de-epoxidation state were enhanced in themutants. The mutants had greate protein/chlorophyll ratio and larger amounts of soluble protein and Rubisco,especially the small subunit of Rubisco. These results demonstrated that the characteristics of two rice mutantsdiffered somewhat from the other common chlorophyll b-less mutants originating from mutagenic agentstreatment.

关键词： Rubisco content chlorophyll absorption spectrum chlorophyll accumulation and degradation carotenoid PS n activity total electron transport rate fractions of photochemistry and thermal dissipation.

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Genetic Engineering of Cassava and Sweet Potato for Industrial Applications

Genetic Engineering of Cassava and Sweet Potato for Industri...

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植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Jun Yang,Shanshan Zhao,Dong An,Huiping Bi,Jia Xu,Qiuxiang Ma,Min Zhang, Jia liu,Peng Zhang~* institute of plant physiology & ecology,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 2.0032,china

Cassava(Manihot esculenta Crantz) and sweetpotato(Ipomoea batatas) accumulate a lot of starch in their storage roots which can be processed to food,feed and bio-ethanol. The high yield potential and robustness against unfavorable environmental conditions make them the suitable energy crops for margin land cultivation,which will not highly compete with other food crops on arable *** are several key biological

关键词： Cassava sweetpotato genetic improvement transgenesis industrialization

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