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QTL Analysis of Major Agronomic Traits in Soybean

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Agricultural Sciences in China 2007年第4期6卷 399-405页

作者： CHEN Qing-shan ZHANG Zhong-chen LIU Chun-yan XIN Da-wei qiu hong-mei SHAN Da-peng SHAN Cai-yun HU Guo-hua Soybean Research Institute Northeast Agricultural University Harbin 150030 P.R.China Land Reclamation Research ＆ Breeding Centre of Heilongjiang Harbin 150036 P.R.China

Soybean is a main crop, and most agronomic traits of soybean are quantitative; therefore, there is vely important studying and applying value to locating these traits. A F2：10 RIL population containing 154 lines, derived from the cross between Charleston as female and Dongnong 594 as male parent, were used in this experiment. A genetic linkage map was constructed with 164 SSR primers, which were screened with the two parents and amplified on the 154 lines. 12 agronomic traits different between the two parents were investigated, and QTLs of all the traits were analyzed using the software Windows QTL Cartographer V2.0. The agronomic traits included quality traits： protein content, oil content, and content of protein and oil; yield traits： pods per plant, seed weight per plant, and 100 seeds weight; and other agronomic traits： plant height, days to maturity, branches, nod number in main stem, average leaf length, and average leaf width. The results showed that 68 QTLs in total were found for the 12 agronomic traits. The number of QTLs per trait varied from 3 for the average leaf width to 11 for 100 seeds weight and plant height, and was 5.8 on average. Good accordance was seen in many QTLs between the results of this study and the results obtained by other similar studies; therefore, these QTLs may be valuable for molecular marker assistant selection in soybean. In this study, 68 major QTLs of 12 important traits of soybean were analyzed.

关键词： soybean agronomic traits QTL SSR

盎格鲁-撒克逊时期城镇的起源及其动力因素分析

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华中科技大学学报（社会科学版） 2017年第3期31卷 67-74页

作者：邱红梅湖北科技学院马克思主义学院

中世纪英国城市的起源、兴起经历了一个漫长的发展过程。英国历史学家詹姆斯·泰特(James Tait)根据考古资料指出,英国最初的城镇产生于盎格鲁-撒克逊时期。本文通过对翻译成现代英语的英国中古文献的研究发现,早在公元7世纪以后,就出... 详细信息

中世纪英国城市的起源、兴起经历了一个漫长的发展过程。英国历史学家詹姆斯·泰特(James Tait)根据考古资料指出,英国最初的城镇产生于盎格鲁-撒克逊时期。本文通过对翻译成现代英语的英国中古文献的研究发现,早在公元7世纪以后,就出现了诸如要塞(ceaster)、贸易小镇(wik、wic、wich)、堡(burh、borough)、市场(port)等多种形式的城镇,而这些城镇的起源是国王与贤人会议、教会、商人、入侵者之间博弈的结果,即国王造就了城镇。对英国中古文献中有关盎格鲁-撒克逊城镇起源的研究结果,印证了英国考古学及泰特的观点,从而为英国城市兴起的研究提供新的视角。

关键词：盎格鲁-撒克逊城镇

自旋梯状化合物Sr_(14)(Cu_(1-x)Zn_x)_(24)O_(41)的电输运及磁学性质

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工程科学学报 2015年第11期37卷 1477-1484页

作者：陈辰邱红梅潘礼庆北京科技大学数理学院北京100083 三峡大学理学院宜昌443002

采用标准固相反应法制备了Sr14（Cu1-xZnx）24O41（x=0,0.01,0.02,0.03）系列多晶样品.X射线衍射谱表明所有样品均呈单相,且样品晶格常数大小随Zn掺杂量x的变化存在微弱波动.X射线光电子能谱表明Sr14Cu24O41中Cu离子以＋2价形式存在,Zn... 详细信息

采用标准固相反应法制备了Sr14（Cu1-xZnx）24O41（x=0,0.01,0.02,0.03）系列多晶样品.X射线衍射谱表明所有样品均呈单相,且样品晶格常数大小随Zn掺杂量x的变化存在微弱波动.X射线光电子能谱表明Sr14Cu24O41中Cu离子以＋2价形式存在,Zn掺杂对体系中Cu离子化合价不造成影响.磁化率测量结果表明在10-300 K温度范围内Zn掺杂使体系磁化率降低,拟合结果表明随着Zn掺杂量x的增大,居里-外斯项对体系磁化率贡献逐渐减弱,二聚体耦合能JD逐渐降低,每个分子中Cu O2自旋链内二聚体个数ND与自由Cu2＋离子自旋数NF均逐渐减少,进一步分析显示替换二聚体内Cu2＋离子的Zn2＋离子数少于替换自由Cu2＋离子的Zn2＋离子数.电阻率测量结果表明Sr14Cu24O41体系具有半导体特性,并且Zn掺杂会使体系电阻率降低,降低程度随掺杂量x增大而增大,但并未使体系发生金属-绝缘体转变.认为电阻率降低可能是由于Zn2＋离子掺杂使体系内Cu O2自旋链中二聚体发生退耦,破坏了电荷有序超结构,从而使更多的空穴释放出来并转移到导电性好的Cu2O3自旋梯子中所致.

关键词：自旋梯状化合物固相反应电输运性质磁学性质磁化率电阻率

Induction of CRMP-4 in striatum of adult rat after transient brain ischemia

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Acta Pharmacologica Sinica 2003年第12期24卷 31-37页

作者： LIU Peng-Chong YANG Zeng-Jin qiu mei-hong, ZHANG Ling-mei, SUN Feng-Yan National Laboratory of Medical Neurobiology, Fudan University Shanghai Medical College, Shanghai 200032, China National Laboratory of Medical Neurobiology Fudan University Shanghai Medical College Shanghai 200032 Chin

AIM: To study the expression of collapsing response mediated protein-4 (CRMP-4) and nestin in the ischemic adult rat brain following transient brain ischemia. METHODS: Brain ischemia was induced by transient left middle cerebral artery occlusion (MCAO) for 60 min in adult rats. The expression of CRMP-4, nestin and bromodeoxyuridine (BrdU) was analyzed by immunohistochemical method. The co-localization of CRMP-4 and nestin or BrdU was analyzed by double staining combined with confocal laser scanning microscopy. RESULTS: CRMP-4, a marker of immature neuron, could be expressed in the ipsilateral striatum and cerebral cortex at 1st and 2nd week after the ischemia-reperfusion; nestin, a marker of neural stem cell, occurred in above regions from several hours to 2 weeks. CRMP-4 costained with nestin and with BrdU incorporation. CONCLUSION: Neural stem cells may present in the striatum and cerebral cortex of adult rat and can be triggered to differentiate into newborn neuron there by ischemic brain trauma.

关键词： brain ischemia corpus striatum CRMP-4 protein intermediate filament proteins bromodeoxyuridine neurons regeneration

“一带一路”背景下农产品贸易实证研究——以中国与中亚五国为例

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技术经济与管理研究 2019年第7期 108-113页

作者：洪秋妹常州工学院

文章首先从贸易规模及结构两方面分析了中国与中亚五国的农产品贸易现状,其次在引力模型的基础上,通过引入“一带一路”政策变量,运用面板数据模型分析了中国与中亚五国农产品贸易的影响因素。研究发现,中国与中亚五国的农产品贸易增幅... 详细信息

文章首先从贸易规模及结构两方面分析了中国与中亚五国的农产品贸易现状,其次在引力模型的基础上,通过引入“一带一路”政策变量,运用面板数据模型分析了中国与中亚五国农产品贸易的影响因素。研究发现,中国与中亚五国的农产品贸易增幅较大,但总量仍偏低,且进出口结构和市场结构有待进一步完善;“一带一路”倡议有利于促进中国与中亚五国的农产品贸易;国内生产总值、人口数量、人均GDP差额、物理距离、农业增加值占比以及两国是否接壤会对农产品贸易产生影响。最后,为更好地促进中国与中亚五国的农产品贸易,提出了相关政策建议。

关键词：一带一路中亚五国农产品贸易

S-亚硝基谷胱甘肽对PGF2α诱导心肌肥大的抑制作用

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重庆医学 2009年第18期38卷 2304-2305,2307,F0002页

作者：邱红梅重庆医科大学药理教研室 400016

目的从细胞水平研究一氧化氮(Nitric oxide,NO)供体S-亚硝基谷胱甘肽(S-nitrosoglutathione,GSNO)对前列腺素F2α(PGF2α)所致心肌肥大的影响,并初步探讨其作用原理。方法利用培养的新生大鼠心肌细胞,以细胞直径大小、蛋白质含量为心肌... 详细信息

目的从细胞水平研究一氧化氮(Nitric oxide,NO)供体S-亚硝基谷胱甘肽(S-nitrosoglutathione,GSNO)对前列腺素F2α(PGF2α)所致心肌肥大的影响,并初步探讨其作用原理。方法利用培养的新生大鼠心肌细胞,以细胞直径大小、蛋白质含量为心肌肥大反应指标,观察药物的抗心肌肥大效应;分别用比色法和荧光法检测细胞NO浓度和游离钙浓度(〔Ca2+〕i),分析其可能的作用原理。结果PGF2α10-7mol/L使心肌细胞明显增大,蛋白质含量明显增加,并使〔Ca2+〕i显著升高,但对NO含量无明显影响。与PGF2α组比较,GSNO10-4mol/L明显使心肌细胞直径、蛋白质含量和〔Ca2+〕i减少;同时使NO浓度明显增加,差异有统计学意义(P<0.05)。结论GSNO可抑制PGF2α诱导的心肌细胞肥大,该作用可能与其释放NO,使心肌细胞局部NO含量增加,从而降低细胞内钙有关。

关键词： S-亚硝基谷胱甘肽前列腺素F2α 心肌肥大一氧化氮钙

GmDRR1,a dirigent protein resistant to Phytophthora sojae in Glycine max (L.) Merr.

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Journal of Integrative Agriculture 2018年第6期17卷 1289-1298页

作者： CHEN Qing-shan YU Guo-long ZOU Jia-nan WANG Jing qiu hong-mei ZHU Rong-sheng CHANG Hui-lin JIANG hong-wei HU Zhen-bang LI Chang-yu ZHANG Yan-jiao WANG Jin-hui WANG Xue-ding GAO Shan LIU Chun-yan QI Zhao-ming FU Yong-fu XIN Da-wei Key Laboratory of Soybean Biology Ministry of Education/College of AgricultureNortheast Agricultural University Key Laboratory of Soybean Biology (Beijing) Ministry of Agriculture/National Key Facility of Crop Gene Resource and Genetic Improvement/Institute of Crop SciencesChinese Academy of Agricultural Sciences

Soil-borne pathogen Phytophthora sojae is an oomycete that causes devastating damage to soybean yield. To mine original resistant genes in soybean is an effective and environmentally-friend approach controlling the disease. In this study, soybean proteins were extracted from the first trifoliolates infected by predominant P. sojae race 1 and analyzed by twodimensional gel electrophoresis. Nineteen differently-expressed protein spots were detected, and 10 of them were further applied for Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry Assay. One protein containing a dirigent （DIR） domain was identified and belonged to the DIR-b/d family. Therefore, it was named as GmDRR1 （Glycine max Disease Resistance Response 1）. Then, GmDRR1 gene was pathologically confirmed to be involved in the resistant to P. sojae in soybean. GmDRR1-GFP （green fluorescent protein） fusion proteins localized in the cell membrane. qRTPCR results showed GmDRR1 gene expressed differently in P. sojae resistant- and susceptible-soybean cultivars. By the promoter analysis, we found a haplotype H8 was existing in most resistant soybean varieties, while a haplotype H77 was existing in most susceptible soybean varieties. The H77 haplotype had seven SNPs （C to A, G to C, C to A, T to A, T to C, T to C, and T to A） and two single nucleotide insertions. The results supported that the expression difference of GmDRR1 genes between P. sojae resistant- and susceptible-soybean cultivars might depend on the GmDRR1 promoter SNPs. The results suggested that GmDRR1 was a dirigent protein involved in soybean resistant to P. sojae and paved a novel way for investigation of the molecular regulatory mechanism of the defense response to P. sojae in soybean.

关键词： dirigent domain GmDRR1 soybean Phytophthora sojae oomycete proteomics

类风湿关节炎病人血清中催乳素水平及与IL-6的关系

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大连医科大学学报 2007年第3期29卷 239-240页

作者：裘红梅李霞大连大学医学院辽宁大连116622

[目的]探讨催乳素在类风湿关节炎(rheum atoid arthritis,RA)发病中的作用。[方法]放射免疫分析法检测RA病人血清中催乳素及白细胞介素6(IL-6)的水平。[结果]RA组与对照组血清中催乳素水平分别为(9.9±3.0)g/L与(8.5±2.4)g/L(P<0.05);I... 详细信息

[目的]探讨催乳素在类风湿关节炎(rheum atoid arthritis,RA)发病中的作用。[方法]放射免疫分析法检测RA病人血清中催乳素及白细胞介素6(IL-6)的水平。[结果]RA组与对照组血清中催乳素水平分别为(9.9±3.0)g/L与(8.5±2.4)g/L(P<0.05);IL-6的水平分别为(27.8±7.5)pg/mL与(15.6±3.2)pg/mL(P<0.01)。催乳素水平与IL-6呈正相关关系(r=0.52;P<0.01)。[结论]催乳素可能是导致RA发病的一个因素,且与IL-6可能存在着相互调节的作用。

关键词：类风湿关节炎催乳素白细胞介素6

胶原性关节炎大鼠血清中TNF-α,IL-17及IL-10水平分析

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大连医科大学学报 2012年第5期34卷 492-494页

作者：宋光裘红梅大连大学医学院检验系辽宁大连116622

[目的]检测血清TNF-α,IL-17及IL-10在Ⅱ型胶原诱导性关节炎(CIA)大鼠血清中的表达。[方法]建立CIA动物模型,用酶联免疫吸附实验(ELISA)法检测大鼠模型血清中炎性细胞因子TNF-α、IL-10及IL-17的水平,同时观察大鼠炎症指数。[结果]60只... 详细信息

[目的]检测血清TNF-α,IL-17及IL-10在Ⅱ型胶原诱导性关节炎(CIA)大鼠血清中的表达。[方法]建立CIA动物模型,用酶联免疫吸附实验(ELISA)法检测大鼠模型血清中炎性细胞因子TNF-α、IL-10及IL-17的水平,同时观察大鼠炎症指数。[结果]60只大鼠随机被分为造模组(40只)和对照组(20只),免疫4周后,造模组40只大鼠有32只发病。造模成功组大鼠血清中TNF-α、IL-17水平分别为(39.65±11.23)pg/mL和(46.1±10.9)pg/mL,明显高于造模失败组和正常对照组(P均<0.05),而IL-10水平(29.11±27.59)pg/mL明显低于造模失败组和正常对照组(P<0.05)。另外,造模组大鼠血清TNF-α和IL-17水平与CIA大鼠炎症指数呈正相关关系(r=0.573,r=0.492,P<0.05),与之相反,CIA大鼠血清IL-10水平与炎症指数呈负相关关系(r=-0.481,P<0.05)。[结论]致炎因子(TNF-α,IL-17)和抑炎因子(IL-10)表达失衡可能在CIA发病中具有重要作用。

关键词：胶原性关节炎 TNF-α IL-17 IL-10 关节炎症指数