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Effects of methamidophos and deltamethrin on in vitro protein phosphorylation in Monochamus alternatus

Insect Science 2008年第6期15卷 529-535页

作者： Jie Liu Xi-Wu Gao Yi-Jun Wu Wei Li qi-lian qin Jiang-Hua Sun State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences Beijing College of Agronomy and Biotechnology China Agricultural University Beijing China

Monochamus alternatus Hope （Coleoptera： Cerambycidae） is not only a serious pest insect to pine trees but also the main vector of pine wood nemadote Bursaphelenchus xylophilus, which causes pine wilt disease. To explore the insecticidal mechanism of insecticides to M. alternatus, we chose methamidophos and deltamethrin as the representatives of two groups of insecticides （organophosphates and pyrethroids）, which are widely used for pest control in China and investigated their effects on phosphorylation of proteins from the insect. Phosphorylation of proteins from the insect fat body and head was determined by in vitro 32P-labelling. In the fat body, deltamethrin obviously reduced basal phosphorylation levels of proteins at 111, 95, 77, and 44 kDa, but enhanced the basal phosphorylation level of a protein at 138 kDa. However, in the presence of calmodulin but not cyclic adenosine monophosphate （cAMP）, deltamethrin increased phosphorylation of the protein at 111 kDa. In the head, deltamethrin inhibited basal phosphorylation levels of proteins at 113, 98, and 51 kDa, but potentiated phosphorylation of a protein at 167 kDa activated by cAMP. Methamidophos inhibited phosphorylation of a protein at 44 kDa in the fat body. Although methamidophos did not impact basal phosphorylation levels of any proteins in the head, it inhibited calcium/calmodulin （Ca^2＋CaM）-stimulated phosphorylation of a protein at 51 kDa. Together, our data indicate that methamidophos and deltamethrin altered phosphorylation levels of various proteins in the head and fat body of the pine insect and these two kinds of insecticides acted on the proteins that can be phosphorylated in the tissues respectively, which is possibly related to their toxicity.

关键词： methamidophos deltamethrin Monochamus alternatus protein phosphorylation

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Comparative analysis of C-type lectin domain proteins in the ghost moth, Thitarodes xiaojinensis (Lepidoptera: Hepialidae)

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Insect Science 2019年第3期26卷 453-465页

作者： qian Meng Ji-Hong Zhang Huan Zhang Gui-Ling Zhou Ruo-Yao Ni Yan-Ni Zhao qi-lian qin Zhen Zou State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences Beijing China College of Life Sciences Hebei University Baoding Hebei China University of Chinese Academy of Sciences Beijing China

Insects have a large family of C-type lectins involved in cell adhesion, pathogen recognition and activation of immune responses. In this study, 32 transcripts encoding C-type lectin domain proteins (CTLDPs) were identified from the Thitarodes xiaojinensis transcriptome. According to their domain structures, six CTLDPs with one carbohydraterecognition domain (CRD) were classified into the CTL-S subfamily. The other 23 CTLDPs with two CRDs were grouped into the immulectin (IML) subfamily. The remaining three with extra regulatory domains were sorted into the CTL-X subfamily. Phylogenetic analysis showed that CTL-S and CTL-X members from different insects could form orthologous groups. In contrast, no T. xiaojinensis IML orthologues were found in other insects. Remarkable lineage-specific expansion in this subfamily was observed reflecting that these CTLDPs, as important receptors, have evolved diversified members in response to a variety of microbes. Prediction of binding ligands revealed that T. xiaojinensis, a coldadapted species, conserved the ability of CRDs to combine with Ca^2+ to keep its receptors from freezing. Comparative analysis of induction of CTLDP genes after different immune challenges indicated that IMLs might play critical roles in immune defenses. This study examined T. xiaojinensis CTLDPs and provides a basis for further studies of their characteristics.

关键词： binding ligand carbohydrate-recognition domain C-type lectin domain protein expression profiling phylogenetic feature Thitarodes xiaojinensis

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Metabolomics reveals the key role of oxygen metabolism in heat susceptibility of an alpine-dwelling ghost moth, Thitarodes xiaojinensis (Lepidoptera: Hepialidae)

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Insect Science 2019年第4期26卷 695-710页

作者： Wei Zhu qian Meng Huan Zhang Meng-Long Wang Xumn Li Hong-Tuo Wang Gui-Ling Zhou Lin Miao qi-lian qin Ji-Hong Zhang State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences Beijing China Department of Herpetology Chengdu Institute of Biology Chinese Academy of Sciences Chengdu China

Ghost moths inhabiting the alpine meadows of the Tibetan Plateau are cold? adapted stenothermal organisms that are susceptible to heat (dead within 7 days at 27 °C exposure). Exploring the metabolic basis of their heat susceptibility would extend our understanding of the thermal biology of alpine-dwelling invertebrates. Here, gas chromatography-mass spectrometry-based metabolomics was combined with physiological and transcriptional approaches to determine the metabolic mechanisms of heat susceptibility in Thitarodes xiaojinensis larvae. The metabolomics results showed that 27 °C heat stress impaired the Krebs cycle and lipolysis in T. xiaojinensis larvae, as demonstrated by the accumulation of intermediary metabolites. In addition, carbohydrate reserves were highly and exclusively consumed, and an anaerobic product, lactate, accumulated. This evidence suggested a strong reliance on glycolysis to anaerobically generate energy. The respiration rate and enzymatic activity test results indicated a deficiency in O2 metabolism;in addition, the Krebs cycle capacity was not decreased, and the metabolic flux through aerobic pathways was limited. These findings were further supported by the occurrence of hypoxia symptoms in midgut mitochondria (vacuolation and swelling) and increased transcription of hypoxia-induced factor 1-α.Overall, heat stress caused O2 limitation and depressed the overall intensity of aerobic metabolism in ghost moths, and less efficient anaerobic glycolysis was activated to sustain their energy supply. As carbohydrates were depleted, the energy supply became deficient. Our study presents a comprehensive metabolic explanation for the heat susceptibility of ghost moths and reveals the relationship between O2 metabolism and heat susceptibility in these larvae.

关键词： energy metabolism ghost moths metabolomics thermal intolerance oxygen limitation respiration rate

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Succinylation modification:a potential therapeutic target in stroke

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Neural Regeneration Research 2024年第4期19卷 781-787页

作者： Jie lian Wenwu Liu qin Hu Xiaohua Zhang Department of Neurosurgery Ren Ji HospitalShanghai Jiao Tong University School of Medicine Department of Diving and Hyperbaric Medicine Naval Medical CenterNaval Medical University

Stroke is a leading cause of mortality and disability *** cell death triggered by the compromised supply of blood oxygen and glucose is one of the major pathophysiology of strokeinduced brain *** mitochondrial energy metabolism is observed minutes after stroke and is closely associated with the progression of ***,a new type of posttranslational modification,known as lysine succinylation,has been recognized to play a significant role in mitochondrial energy metabolism after ***,the role of succinylation modification in cell metabolism after stroke and its regulation are not well *** aimed to review the effects of succinylation on energy metabolism,reactive oxygen species generation,and neuroinflammation,as well as Sirtuin 5 mediated desuccinylation after *** also highlight the potential of targeting succinylation/desuccinylation as a promising strategy for the treatment of *** succinylation level is dynamically regulated by the nonenzymatic or enzymatic transfer of a succinyl group to a protein on lysine residues and the removal of succinyl catalyzed by *** evidence has suggested that succinylation can regulate the metabolic pathway through modulating the activity or stability of metabolic ***,especially Sirtuin 5,are characterized for their desuccinylation activity and have been recognized as a critical regulator of metabolism through desuccinylating numerous metabolic *** between succinylation and desuccinylation has been implicated in the pathophysiology of *** agents that enhance the activity of Sirtuin 5 have been employed to promote desuccinylation and improve mitochondrial metabolism,and neuroprotective effects of these agents have been observed in experimental stroke ***,their therapeutic efficacy in stroke patients should be validated.

关键词： mitochondria metabolism neuroprotection sirtuin 5 stroke succinylation modification

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CoFe_(2)O_(4) decorated graphene/C_(18)-functionalized mesoporous silica nanocomposites prepared for magnetic enrichment and electrochemical detection of promethazine in beef

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Chinese Chemical Letters 2024年第3期35卷 208-213页

作者： Xingdong Yang Shi-Ming Ying Sen Zhang Jinxia Dai Wei Gao Tian-qi Wang Jun-qin qiao Hong-Zhen lian Li Mao State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry&Chemical Engineering and Centre of Materials AnalysisNanjing UniversityNanjing 210023China Ministry of Education(MOE)Key Laboratory of Modern Toxicology School of Public HealthNanjing Medical UniversityNanjing 211166China

Promethazine(PHZ)is used as a sedative in veterinary medicine,and its residue can threaten the health of *** electrochemical detection of PHZ is suitable method for application in the ***,the traditional electroanalysis is difficult to perform directly in meat samples due to matrix *** work integrates magnetic solid-phase extraction and differential pulse voltammetry for highly sensitive and selective determination of PHZ in beef and beef liver for the first ***_(2)O_(4)/graphene coated with C_(18)-functionalized mesoporous silica(MG@mSiO_(2)-C_(18))is synthesized as dispersed magnetic adsorbent to extract *** glassy carbon electrode modified with nitrogen-doped hollow carbon microspheres(HCM)attracts the MG@mSiO_(2)-C_(18)with PHZ,and directly detects the PHZ without elution ***@mSiO_(2)-C_(18)can separate PHZ to avoid the interference of impurities on following detection,and also concentrate PHZ on magnetic ***,the electrode modification with HCM can amplify the electrochemical signal of ***,the integrated PHZ determination method exhibits a wide linear range from 0.08μmol/L to 300μmol/L with a low limit of detection of 9.8 nmol/*** beef sample analysis presents excellent recovery,demonstrating that this protocol is promising for the rapid and onsite detection of PHZ in real meat samples。

关键词： Promethazine(PHZ) Drug residue Magnetic solid phase extraction Electrochemical detection Beef

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Cobalt(Ⅲ)hydride HAT mediated enantioselective intramolecular hydroamination access to chiral pyrrolidines

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Science China Chemistry 2024年第6期67卷 2002-2008页

作者： qi Meng Tao qin Huanran Miao Ge Zhang qian Zhang Jilin Province Key Laboratory of Organic Functional Molecular Design&Synthesis Department of ChemistryNortheast Normal UniversityChangchun130024China State Key Laboratory of Organometallic Chemistry Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai200032China

Enantioenriched pyrrolidines and derivatives are ubiquitous substructures in compounds of importance to medicinal and biological ***,we report an efficient cobalt-catalyzed intramolecular asymmetric hydroamination reaction that produces chiral pyrrolidines with good to excellent yield and *** with previously reported radicalinvolved methodologies for enantioenriched pyrrolidines,these conditions feature two elegant versatilities,enabling(1)the use of cobalt-catalyzed hydrogen atom transfer(HAT)to generate organocobalt intermediates that bring radical reaction to organometallic chemistry,and(2)enantioselective intramolecular C-N bond forging through an S_(N)2-like displacement involving dynamic kinetic resolution(DKR).This approach provides a new alternative and efficient methodology for enantioselective radical-involved C-N bond construction that can be used in the synthesis of both chiral pyrrolidines and homologous nitrogen heterocycles.

关键词： asymmetric catalysis radical hydroamination cobalt catalysis hydrogen atom transfer

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Performance analysis of the tight combination of GPS, BDS, GALILEO, and QzsS mixed frequencies signals

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Geodesy and Geodynamics 2024年第4期15卷 366-378页

作者： Yu Han Fangjun qin Xugang lian Shengliang Wang Leiyuan qian Wenhua Huang College of Electrical Engineering Naval University of EngineeringWuhan 430033China College of Mining Engineering Taiyuan University of TechnologyTaiyuan 030024China State Key Laboratory of Satellite Navigation System and Equipment Technology Shijiazhuang 050081China State Key Laboratoryof Geodesy and Earth'sDynamics Innovation AcademyforPrecision Measurement Scienceand Technology Chinese Academy of SciencesWuhan 430077China China Energy Engineering Group Shanxi Electric Power Engineering CO.LTD. Taiyuan 030001China

With the gradual development and modernization of satellite navigation systems,using observation information from multi-GNss has become one of the hot-spot issues in recent ***-system loose combinations form double-difference observation equations within their respective systems,and the positioning effect is ***,the interchangeability and compatible interoperability between global navigation satellite systems(GNSS)cannot be truly *** the same time,when the number of visible satellites decreases abruptly,the positioning performance deteriorates *** paper focuses on the GNsS multi-system tight combination relative positioning technique,gives a mathe-matical model of multi-system tight combination relative positioning considering differential inter-system bias(DISB),and analyzes the time-varying characteristics of DISB at overlapping and non-overlapping frequencies among GPS/Galileo,GPS/BDS,and GPS/QZSS in terms of receiver brand,tem-perature,and receiver *** GNsS tight combination relative positioning performance is verified by static data from Curtin University and dynamic data measured at Taiyuan University of *** results show that compared with loose combination,the ambiguity-fixed rate increases from 62.18%to 97.60%for static data and from 74.97%to 99.53%for dynamic data when the elevation mask angle is 50°,resulting in a significant improvement in positioning performance.

关键词： GNSS Mix frequencies Redundancy Tightcombination Differential inter-system bias

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Phase-field lattice-Boltzmann study on fully coupled thermal-solute-convection dendrite growth of Al-Cu alloy

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China Foundry 2024年第2期21卷 125-136页

作者： Yin-qi qiu Meng-wu Wu Xun-peng qin Shou-mei Xiong Hubei Key Laboratory of Advanced Technology for Automotive Components Wuhan University of TechnologyWuhan 430070China Hubei Longzhong Laboratory Xiangyang 441000HubeiChina School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

Dendrite growth is a complex liquid-solid phase transition process involving multiple physical factors.A phase-field lattice-Boltzmann method was developed to simulate the two-and three-dimension dendrite growth of Al-Cu *** effect of fully coupled thermal-solute-convection interaction on the dendrite growth was investigated by incorporating a parallel-adaptive mesh refinement algorithm into the numerical *** accurately reproducing the latent heat release,solute diffusion and convective transport behaviors at the liquidsolid interface,the interaction mechanism among thermal-solute-convection transport as well as their coupling effects on the dendrite growth dynamics were *** simulation results show that the release of latent heat slows down the dendrite growth rate,and both natural and forced convection disrupt the symmetrical growth of *** combination makes the growth of dendrites more complex,capturing important physical aspects such as recalescence,dendrite tip splitting,dendrite tilting,dendrite remelting,and solute plume in the simulation *** on the robustness and powerful ability of the numerical model,the formation mechanisms of these physical aspects were revealed.

关键词： simulation phase field dendrite growth thermal-solute-convection interaction

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Risk Assessment and Defense Resource Allocation of Cyber-physical Distribution Systems Under Denial-of-service Attacks

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CSEE Journal of Power and Energy Systems 2024年第5期10卷 2197-2207页

作者： Han qin Jiaming Weng Dong Liu Donglian qi Yufei Wang School of Electronic Information and Electrical Engineering Shanghai Jiao Tong UniversityShanghai 200240China School of Electrical Engineering Zhejiang UniversityHangzhou 310027China China Electric Power Research Institute Beijing 102209China

With the help of advanced information technology,real-time monitoring and control levels of cyber-physical distribution systems(CPDS)have been significantly *** due to the deep integration of cyber and physical systems,attackers could still threaten the stable operation of CPDS by launching cyber-attacks,such as denial-of-service(DoS)***,it is necessary to study the CPDS risk assessment and defense resource allocation methods under DoS *** paper analyzes the impact of DoS attacks on the physical system based on the CPDS fault self-healing ***,considering attacker and defender strategies and attack damage,a CPDS risk assessment framework is ***,risk assessment and defense resource allocation methods,based on the Stackelberg dynamic game model,are proposed under conditions in which the cyber and physical systems are launched ***,a simulation based on an actual CPDS is performed,and the calculation results verify the effectiveness of the algorithm.

关键词： Cyber physical distribution system defense resource allocation denial-of-service attack risk assessment Stackelberg dynamic game model

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Study of three-dimensional spatial diffuse discharge in contact electrode structure applied to air purification

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Plasma Science and Technology 2024年第10期26卷 73-81页

作者： Shuai XU Wenzheng LIU Jiaying qin Yiwei SUN Xitao JIANG qi qi School of Electrical Engineering Beijing Jiaotong UniversityBeijing 100044People’s Republic of China

In this work,based on the role of pre-ionization of the non-uniform electric field and its effect of reducing the collisional ionization coefficient,a diffuse dielectric barrier discharge plasma is formed in the open space outside the electrode structure at a lower voltage by constructing a three-dimensional non-uniform spatial electric field using a contact electrode *** air purification study is also carried ***,a contact electrode structure is constructed using a three-dimensional wire *** distribution characteristics of the spatial electric field formed by this electrode structure are analyzed,and the effects of the non-uniform electric field and the different angles of the vertical wire on the generation of three-dimensional spatial diffuse discharge are ***,the copper foam contact electrode structure is constructed using copper foam material,and the effects of different mesh sizes on the electric field distribution are *** results show that as the mesh size of the copper foam becomes larger,a strong electric field region exists not only on the surface of the insulating layer,but also on the surface of the vertical wires inside the copper foam,i.e.,the strong electric field region shows a three-dimensional ***,as the mesh size increases,the area of the vertical strong electric field also ***,the electric field strength on the surface of the insulating layer gradually ***,the appropriate mesh size can effectively increase the discharge area,which is conducive to improving the air purification ***,a highly permeable stacked electrode structure of multilayer wire-copper foam is *** combination with an ozone treatment catalyst,an air purification device is fabricated,and the air purification experiment is carried out.

关键词： dielectric barrier discharge three-dimensional spatial discharge atmospheric pressure air diffusion discharge air purification

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