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Establishing Modular Cell-Free Expression System for the Biosynthesis of Bicyclomycin from a Chemically Synthesized Cyclodipeptide

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Chinese Journal of Chemistry 2024年第4期42卷 384-390页

作者： Yi-Pei Liu Yu-Heng Zhao wen-qi Zhang Lian Wu Linjuan Huang Gong-Li tang Jun-Bin He Shaanxi Natural Carbohydrate Resource Engineering Research Center College of Food Science and TechnologyNorthwest UniversityXi'anShaanxi710069 China State Key Laboratory of Chemical Biology Shanghai Institute of Organic ChemistryUniversity of Chinese Academy of Sciences(CAS)CASShanghai200032 China School of Chemistry and Materials Science Hangzhou Institute for Advanced StudyUniversity of CAS1 Sub-lane XiangshanHangzhouZhejiang310024 China

Cell-free expression systems have emerged as a versatile and powerful platform for metabolic engineering,biosynthesis and synthetic biology ***,successful examples of the synthesis of complex natural products using this system are still ***,a structurally unique and complex diketopiperazine alkaloid,is a clinically promising antibiotic that selectively inhibits the transcription termination factor ***,we established a modular cell-free expression system with cascade catalysis for the biosynthesis of bicyclomycin from a chemically synthesized *** six cell-free expressed biosynthetic enzymes,including five iron-andα-ketoglutarate-dependent dioxygenases and one cytochrome P450 monooxygenase,were active in converting their substrates to the corresponding *** co-expressed enzymes in the cell-free module were able to complete the related partial *** vitro biosynthesis of bicyclomycin was also achieved by reconstituting the entire biosynthetic pathways(i.e.,six enzymes)using the modular cell-free expression *** study demonstrates that the modular cell-free expression system can be used as a robust and promising platformforthe biosynthesis of complex antibiotics.

关键词： Natural products Bicyclomycin Biosynthesis Iron-andα-ketoglutarate-dependent dioxygenases Cytochrome P450 monooxygenase Cell-freeprotein synthesis Enzyme catalysis

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AGK2 pre-treatment protects against thioacetamide-induced acute liver failure via regulating the MFN2-PERK axis and ferroptosis signaling pathway

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Hepatobiliary & Pancreatic Diseases International 2024年第1期23卷 43-51页

作者： qing-qi Zhang qian Chen Pan Cao Chun-Xia Shi Lu-Yi Zhang Lu-wen Wang Zuo-Jiong Gong Department of Infectious Diseases Renmin Hospital of Wuhan UniversityWuhan 430060China

Background:Acute liver failure(ALF)is an unpredictable and life-threatening critical *** pathological characteristic of ALF is massive necrosis of hepatocytes and lots of inflammatory cells infiltration which may lead to multiple organ ***:Animals were divided into 3 groups,normal,thioacetamide(TAA,ALF model)and TAA+*** L02 cells were divided into 5 groups,normal,TAA,TAA+mitofusin 2(MFN2)-siRNA,TAA+AGK2,and TAA+AGK2+MFN2-siRNA *** liver histology was evaluated with hematoxylin and eosin staining,inositol-requiring enzyme 1(IRE1),activating transcription factor 6β(ATF6β),protein kinase R(PKR)-like endoplasmic reticulum kinase(PERK)and phosphorylated-PERK(p-PERK).C/EBP homologous protein(CHOP),reactive oxygen species(ROS),MFN2 and glutathione peroxidase 4(GPX4)were measured with Western blotting,and cell viability and liver chemistry were also *** endoplasmic reticulum membranes(MAMs)were measured by ***:The liver tissue in the ALF group had massive inflammatory cell infiltration and hepatocytes necrosis,which were reduced by AGK2 *** comparison to the normal group,apoptosis rate and levels of IRE1,ATF6β,p-PERK,CHOP,ROS and Fe2+in the TAA-induced ALF model group were significantly increased,which were decreased by AGK2 *** levels of MFN2 and GPX4 were decreased in TAA-induced mice compared with the normal group,which were enhanced by AGK2 *** with the TAA-induced L02 cell,apoptosis rate and levels of IRE1,ATF6β,p-PERK,CHOP,ROS and Fe2+were further increased and levels of MFN2 and GPX4 were decreased in the MFN2-siRNA ***2 pre-treatment decreased the apoptosis rate and levels of IRE1,ATF6β,p-PERK,CHOP,ROS and Fe2+and enhanced the protein expression of MFN2 and GPX4 in MFN2-siRNA treated L02 *** observation showed that level of MAMs was promoted in the AGK2 pre-treatment group when compared with the TAA-induced gr

关键词： SIRT2 inhibitor AGK2 Acute liver failure MFN2 Ferroptosis

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Damping capacity of Fe_(83)Ga_(17) magnetostrictive alloy under magnetic field

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Rare Metals 2024年第1期43卷 402-409页

作者： Shao-wen Yan Xing Mu Bing-Jie Liu Li-Hong Xu Shi-Hai Guo Yan qi Hong-Ping Zhang Department of Functional Material Research Central Iron and Steel Research InstituteBeijing 100081China

The damping performance of Fe_(83)Ga_(17) alloys has been investigated in the static magnetic field (H). The results demonstrate that the damping peak value can be adjusted between 0.020 and 0.085 under the magnetic f... 详细信息

关键词： alloy magnetic alloys

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Insight study of rare earth elements in PM_(2.5)during five years in a Chinese inland city:Composition

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Journal of Environmental Sciences 2024年第4期138卷 439-449页

作者： Yi-wen Shen Chang-Xian Zhao Hao Zhao Shuo-Fei Dong qi Guo Jiao-Jiao Xie Mei-Ling Lv Chun-Gang Yuan Hebei Key Lab of Power Plant Flue Gas Multi-Pollutants Control Department ofEnvironmental Science&EngineeringNorth China Electric Power UniversityBaoding 071000China MOE Key Laboratory of Resources and Environmental Systems Optimization College of Environmental Science and EngineeringNorth China Electric Power UniversityBeijing 102206China Agilent Technologies Co.Ltd.(China) Beijing 100102China

The booming development of rare earth industry and the extensive utilization of its products accompanied by urban development have led to the accelerated accumulation of rare earth elements(REEs)as emerging pollutants in atmospheric *** this study,the variation of REEs in PM_(2.5)with urban(a non-mining city)transformation was investigated through five consecutive years of sample *** compositional variability and provenance contribution of REEs in PM_(2.5)were characterized,and the REEs exposure risks of children and adults via inhalation,ingestion and dermal absorption were also *** results showed an increase in the total REEs concentration from 46.46±35.16 mg/kg(2017)to 81.22±38.98 mg/kg(2021)over the five-year period,with Ce and La making the largest *** actual increment of industrial and traffic emission source among the three pollution sources was 1.34 ng/m^(3).Coal combustion source displayed a downward *** was the main exposure pathway for REEs in PM_(2.5)for both children and *** contributed the most to the total intake of REEs in PM_(2.5)among the population,followed by La and *** exposure risks of REEs in PM_(2.5)in the region were relatively low,but the trend of change was of great *** was strongly recommended to strengthen the concern about traffic-related non-exhaust emissions of particulate matter.

关键词： Rare earth elements Exposure risks Pollution sources Traffic-related non-exhaust emissions

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Surface engineering on MnO_(2)nanorods by La single atoms to accelerate oxygen reduction kinetics

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Rare Metals 2024年第9期43卷 4302-4311页

作者： Zhang-Long He Liu-qi Wang Min Jiang Jia-Nan Xie Shan Liu Jin-Can Ren Rui Sun wen-Bin Lv Wei-Bin Guo Yu-Ling Liu Bing Li qi Liu Hao He School of Materials Science and Engineering Changsha University of Science and TechnologyChangsha 410000China School of Materials Science and Engineering Nanjing University of Science and TechnologyNanjing 210094China School of Materials Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China Department of Physics City University of Hong KongHong Kong 999077China

Surface engineering,which modulates the electronic structure and adsorption/desorption properties of electrocatalysts,is one of the key strategies for improving the catalytic ***,we demonstrate a facile solid-phase reaction for surface engineering of MnO_(2)to boost the oxygen reduction *** reaction with surface hydroxy groups,La single atoms with loading amount up to 2.7 wt%are anchored onto a-MnO_(2)*** surface engineering,the oxygen reduction reaction(ORR)kinetics is significantly improved with the half-wave potential from 0.70 to 0.84 V,the number of transferred electrons from 2.5 to 3.9 and the limiting current density from 4.8 to 6.0 mA·cm^(-2).In addition,the catalyst delivers superior discharge performance in both alkaline and neutral metal–air *** functional theory(DFT)calculations reveal that atomic La modulates the surface electronic configuration of MnO_(2),reduces its d-band center and thus lowers the OOH*and O*reaction energy *** work provides a new route for rational design of highly active electrocatalyst and holds great potential for application in various catalytic reactions.

关键词： Surface engineering Single atoms Electron injection Oxygen reduction reaction Metal-air batteries

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Effect of SARS-CoV-2 infection on semen parameters in sperm bank volunteers with normal sperm concentration

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Asian Journal of Andrology 2024年第3期26卷 328-332页

作者： Li Wang wen-Kai Han Xiao-Hui Song qi-Lin Zhang An-Quan Guan Yuan Gao Jin-Long Ma Center for Reproductive Medicine Shandong UniversityJinan 250001China State Key Laboratory of Reproductive Medicine and Offspring Health ShandongUniversityJinan 250001China Key Laboratory of Reproductive Endocrinology of Ministry of Education Shandong UniversityJinan 250001China Shandong KeyLaboratory of Reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250001China Shandong Provincial Clinical Research Center for Reproductive Health Jinan 250001China Shandong Technology Innovation Center for Reproductive Health Jinan 250001China NationalResearch Center for Assisted Reproductive Technology and Reproductive Genetics Shandong UniversityJinan 250001China Research Unit of Gametogenesis andHealth of ART-Offspring Chinese Academy of Medical Sciences(No.2021RU001)Jinan 250001China

In this study,we aimed to assess the effect of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection on *** study comprised 110 sperm volunteers who self-reported SARS-CoV-2 infection from the Human Sperm Bank ofthe Center for Reproductive Medicine,Shandong University(Jinan,China).The volunteers had normal sperm concentration *** volunteer provided semen samples before and after *** selected 90 days after infection as the cutoff *** parameters within 90 days after infection of 109 volunteers(group A)were compared with semen parameters before ***,semen parameters on or after 90 days after infection of 36 volunteers(group B)were compared with semen parameters ***,based on whether the volunteers had completed the three-dose SARS-CoV-2 vaccination booster,volunteersin group A and B were further divided into two subgroups *** parameters were compared before and after infection ineach *** results showed that in this cohort population,the semen quality in volunteers with normal sperm concentrationsbefore infection decreased after SARS-CoV-2 infection within 90 days,while the semen quality returned to preinfection levels after *** completion of a three-dose SARS-CoV-2 vaccination booster may exert a protective effect on semen quality after infection.

关键词： SARS-CoV-2 SARS-CoV-2 vaccination semen quality sperm donation sperm parameters

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Photoexcitation of Dihydroquinazolinone Anionic Compounds:Difluoroalkylarylation and Difluoroalkylamination of Alkenes

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CCS Chemistry 2024年第10期6卷 2549-2559页

作者： Jian-Xiong Yang Jia-qing Hu qi wen Jie Xu Yan Zhang Heng Jiang School of Chemistry and Chemical Engineering Chemistry and Biomedicine Innovation Center(Chem BIC)Nanjing UniversityNanjing 210023 Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs Shanghai Frontiers Science Center of Drug Target Identification and DeliverySchool of Pharmaceutical SciencesShanghai Jiao Tong UniversityShanghai 200240

The direct excitation of dihydroquinazolinone(DHQ)anions by visible light has been demonstrated by photophysical *** excited-state DHQ anion serves as both a potent reductant and a radical precursor in a redox neutral radical cascade,circumventing the use of an external photoredox *** gem-difluoroalkyl radicals(CF_(2)H and CF_(2)R)are produced from the excited-state DHQ anions,leading to the first example of intermolecular 1,2-difluoroalkylarylation(CF_(2)H-and CF_(2)R-arylation)of alkenes in overall redox-neutral ***,1,2-difluoroalkylamination of styrenes has also been realized,offering a practical pathway for the preparation of diverseβ-CF_(2)H *** general and efficient strategy for the generation of CF_(2)H and CF_(2)R radicals exhibits great potential for applications in drug discovery.

关键词： excited state anion difluoromethyl radical difluoromethylation photochemistry radical chemistry

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Effect of Guanxin Danshen Dripping Pills on Coronary Heart Disease Comorbid with Depression or Anxiety: The ADECODE-Real World Study

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Chinese Journal of Integrative Medicine 2024年第5期30卷 443-448页

作者： WU Shi-hao SHI Wei-qi LI Yu-hang LIU Ru-hui HU Da-yi ZHENG Li-qiang MA wen-lin Department of Geriatric Medicine Tongji HospitalSchool of MedicineTongji UniversityShanghai 200065China Department of Cardiology Tongji HospitalSchool of MedicineTongji UniversityShanghai 200065China Department of Cardiology Peking University People's HospitalBeijing 100044China School of Public Health Shanghai Jiao Tong UniversityShanghai 200030China

Objective: To evaluate the efficacy of Guanxin Danshen Dripping Pill(GXDSDP) in treating anxiety and depression in patients with coronary heart disease(CHD). Methods: A total of 1,428 patients diagnosed with CHD screened for anxiety, depression, and quality of life(QOL) at baseline received 0.4 g of GXDSDP treatment 3 times per day and returned for monthly reassessment. Patients were recruited after stable treatment for CHD and received assessment of General Anxiety Disorder-7(GAD-7), Patient Health Questionnaire-9(PHQ-9), and Seattle Angina Questionnaire(SAQ) for evaluating anxiety, depression, and *** were followed up 3 times, once every 4 weeks, during outpatient visits for 12 weeks. Results: At the third follow-up(F3), the anxiety symptom of 63.79%(673/1,055) of the patients improved to sub-clinical level, and the GAD-7 score improved significantly(8.11 vs. 3.87, P<0.01);57.52%(585/1,017) patients' depressive symptoms improved to sub-clinical level, with a significant improvement in PHQ-9 score(8.69 vs. 4.41, P<0.01) at F3. All aspects of QOL significantly improved at the end of treatment compared to those at baseline(all P<0.01) as assessed by SAQ: physical limitation(31.17 vs. 34.14), anginal stability(2.74 vs. 4.14), anginal frequency(8.16vs. 9.10), treatment satisfaction(13.43 vs. 16.29), and disease perception(8.69 vs. 11.02). Conclusions: A fixed dosage of GXDSDP may be a potential treatment option for CHD patients comorbid with anxiety or depression.(Registration No. ChiCTR2100051523)

关键词： Guanxin Danshen Dripping Pill coronary heart disease depression anxiety quality of life Chinese medicine

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Association between the cumulative triglyceride-glucose index and the recurrence of atrial fibrillation after radiofrequency catheter ablation

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Journal of Geriatric Cardiology 2024年第2期21卷 200-210页

作者： qing YAN Jia-qi LIANG Yi-De YUAN Yuan LI Jia-Li FAN wen-Huan WU Pan XU Jia-Hong XUE Department of Cardiovascular Medicine the Second Affiliated Hospital of Xi’an Jiaotong UniversityShaanxiChina Department of Cardiovascular Medicine Zichang People’s HospitalShaanxiChina Department of Ultrasound Xijing HospitalAir Force Military Medical UniversityShaanxiChina

BACKGROUND Triglyceride-glucose(TyG)index values are a new surrogate marker for insulin *** study aimed to explore the relationship between cumulative TyG index values and atrial fibrillation(AF)recurrence after radiofrequency catheter ablation(RFCA).METHODS A total of 576 patients with AF who underwent RFCA at the Second Affiliated Hospital of Xi'an Jiaotong University were included in this *** participants were grouped based on cumulative TyG index values tertiles within 3 months after *** regression and restricted cubic spline analyses were used to determine the relationship between cumulative TyG index values and AF *** predictive value of all risk factors was assessed by receiver operating curve *** There were 375 patients completed the study(age:63.23±10.73 years,64.27%male).The risk of AF recurrence increased with increasing cumulative TyG index values *** adjusting for potential confounders,patients in the medium cumulative TyG index group[hazard ratio(HR)=4.949,95%CI:1.778–13.778,P=0.002]and the high cumulative TyG index group(HR=8.716,95%CI:3.371–22.536,P<0.001)had a higher risk of AF recurrence than those in the low cumulative TyG index *** restricted cubic spline regression model also showed an increased risk of AF recurrence with increasing cumulative TyG index *** considering cumulative TyG index values,left atrial diameter,and lactate dehydrogenase levels as a comprehensive factor,the model could effectively predict AF recurrence after RFCA[area under the curve(AUC)=0.847,95%CI:0.797–0.897,P<0.001].CONCLUSIONS Cumulative TyG index values were a risk factor for AF recurrence after *** longitudinal TyG index values may assist with optimized for risk stratification and outcome prediction for AF recurrence.

关键词： atrial fibrillation index

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Clinical efficacy and pathological outcomes of transanal endoscopic intersphincteric resection for low rectal cancer

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World Journal of Gastrointestinal Oncology 2024年第3期16卷 933-944页

作者： Zhi-wen Xu Jing-Tao Zhu Hao-Yu Bai Xue-Jun Yu qing-qi Hong Jun You Department of Gastrointestinal Oncology Surgery The First Affiliated Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityXiamen 361000Fujian ProvinceChina

BACKGROUND Transanal endoscopic intersphincteric resection(ISR)surgery currently lacks sufficient clinical research and *** To investigate the clinical effectiveness of transanal endoscopic ISR,in order to promote the clinical application and development of this *** This study utilized a retrospective case series *** and pathological data of patients with lower rectal cancer who underwent transanal endoscopic ISR at the First Affiliated Hospital of Xiamen University between May 2018 and May 2023 were *** patients underwent transanal endoscopic ISR as the surgical *** conducted this study to determine the perioperative recovery status,postoperative complications,and pathological specimen charac-teristics of this group of *** This study included 45 eligible patients,with no perioperative *** overall incidence of early complications was 22.22%,with a rate of 4.44%for Clavien-Dindo grade≥III *** patients(4.4%)developed anastomotic leakage after surgery,including one case of grade A and one case of grade *** pathological examination confirmed negative circumferential resection margins and distal resection margins in all *** mean distance between the tumor lower margin and distal resection margin was found to be 2.30±0.62 *** transanal endoscopic ISR procedure consistently yielded high quality pathological *** Transanal endoscopic ISR is safe,feasible,and provides a clear anatomical *** is associated with a low incidence of postoperative complications and favorable pathological outcomes,making it worth further research and application.

关键词： Intersphincteric resection Transanal Rectal cancer Complications Endoscopic

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