检索结果-南通市图书馆

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

基于传统品质评价的淡豆豉质量分析

中国实验方剂学杂志 2024年第1期30卷 31-42页

作者：王艺涵林王敏张水利俞冰南铁贵康利平李国凤何锡远詹志来黄璐琦中国中医科学院中药资源中心道地药材国家重点实验室北京100700 浙江中医药大学药学院杭州310053 成都宝芎中药材有限公司四川彭州611930

目的:基于历代本草总结的淡豆豉“香美而浓者佳”的品质评价经验,对黑豆与淡豆豉所含化学成分进行系统对比分析,并对淡豆豉不同发酵时间的主要发酵产物进行定量分析,以明确炮制过程内在成分的转化规律,为淡豆豉现代质量控制提供科学依... 详细信息

目的:基于历代本草总结的淡豆豉“香美而浓者佳”的品质评价经验,对黑豆与淡豆豉所含化学成分进行系统对比分析,并对淡豆豉不同发酵时间的主要发酵产物进行定量分析,以明确炮制过程内在成分的转化规律,为淡豆豉现代质量控制提供科学依据。方法:采用超高效液相色谱-四极杆-飞行时间质谱法(UPLC-q-TOF-MS)对黑豆及淡豆豉的化学成分进行结构鉴定,借助Progenesis qI v2.3软件,采用负离子模式进行主成分分析(PCA)及正交偏最小二乘法-判别分析(OPLS-DA),绘制S-plot图,选取|P|>0.1的化合物作为备选差异性成分。采用UPLC测定淡豆豉发酵期间异黄酮类化合物含量,前酵期间每8 h取样,后酵期间每2 d取样,并分析不同发酵阶段异黄酮含量动态变化。采取异硫氰酸苯酯(PITC)柱前衍生,利用高效液相色谱法(HPLC)检测淡豆豉不同发酵阶段样品及黑豆中氨基酸和核苷酸的含量,通过味道强度值(TAV值)探讨各呈味物质对淡豆豉“美”味的贡献度。结果:从黑豆和淡豆豉中共筛选出19种差异成分,以大豆皂苷类和异黄酮类成分为主,发酵后含量均显著减少甚至消失。淡豆豉前酵过程主要发生苷键水解反应,黑豆中所含异黄酮苷类物质大量降解转化为对应的苷元类物质,氨基酸等风味物质含量增势较为平缓;后酵过程则主要发生蛋白质降解反应,后酵8 d后异黄酮类化合物含量基本稳定,而各氨基酸总含量平均上升8~40倍,不同的氨基酸形成了淡豆豉特殊的风味,如鲜味物质谷氨酸TAV值始终领先于其他呈味物质,甜味物质如丙氨酸、缬氨酸对淡豆豉的贡献同样较大。结论:基于成分转化规律,结合传统“香美而浓”的评价指标,淡豆豉现有标准较难控制其发酵程度,建议细化淡豆豉性状描述,改为“气香,味鲜,微甘”,同时增加谷氨酸、丙氨酸和缬氨酸等后酵程度指标性化合物为淡豆豉质控指标,以规范淡豆豉制作工艺,提高淡豆豉质量。

关键词：淡豆豉黑豆品质评价超高效液相色谱-四极杆飞行时间串联质谱法(UPLC-q-TOF-MS) 异黄酮氨基酸呈味物质

Soil Cellulase Activity and Fungal Community Responses to Wetland Degradation in the Zoige Plateau, China

在线全文

学校读者我要写书评

暂无评论

Journal of Mountain Science 2015年第2期12卷 471-482页

作者： WU li-sha FENG Su NIE Yuan-yang ZHOU Jian-hong YANG Zhi-rong ZHANG Jie Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education College of Life SciencesSichuan University State Key Laboratory of Hydraulics and Mountain River Engineering Sichuan University

Four soil types(peat, marsh, meadow, and sandy) in the Zoige Plateau of China are associated with the severity of wetland degradation. The effects of wetland degradation on the structure and abundance of fungal communities and cellulase activity were assessed in these 4 soil types at 3 depths using DGGE(Denatured Gradient Gel Electrophoresis), q PCR(quantitative Real-time PCR),and 3,5-dinitrosalicylic acid assays. Cellulase activity and abundance of the fungal community declined in parallel to the level of wetland degradation(from least to most disturbed). DGGE analysis indicated a major shift in composition of fungal communities among the4 soil types consistent with the level of *** content(WC), organic carbon(OC), total nitrogen(TN), total phosphorus(TP), available nitrogen(AN), and available phosphorus(AP) were strongly correlated with cellulase activity and the structure and abundance of the fungal *** results indicate that soil physicochemical properties(WC, OC, TN, TP, AN, and AP), cellulase activity, and diversity and abundance of fungal communities are sensitive indicators of the relative level of wetland degradation. WC was the major factorinvolved in Zoige wetland degradation and lower WC levels contributed to declines in the abundance and diversity of the fungal community and reduction in cellulase activity.

关键词： Wetland degradation Soil cellulase Fungal community DGGE(Denatured Gradient Gel Electrophoresis) qPCR(quantitative Real-time PCR)

Protective Effect of Holothurian Intestine Against Indomethacin Induced Gastric Mucosal Damage in Rats

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2017年第3期16卷 547-554页

作者： li xiaoyu qIAO xuejing ZHANG Cuiping GAO Hua NIU qinghui WU Tong ZHANG qi TIAN Zibin Department of Gastroenterology the Affiliated Hospital of Qingdao University Department of Pharmacy College of Medicine of Qingdao University

Our study aimed to investigate the protective effects of Holothurian intestines(HI) on NSAIDs-induced gastric mucosal damage and the possible mechanism. At first, 60 male Wistar rats were induced of gastric lesions with indomethacin(IDM, 30 mg kg^(-1)). The rats were pretreated for 15 consecutive days with saline, sucralfate, or HI(0.4g kg^(-1) d-1, 0.8 g kg^(-1) d^(-1) and 1.6 g kg^(-1) d^(-1)) prior to IDM treatment, followed by evaluations of macroscopic damage and microscopic features; and investigation of the levels of inflammatory cytokines, oxidative stress parameters, gastric mucosal prostaglandin E2(PGE2) and total hexosamine in tissues. The expression of COx-1 and COx-2 m RNA in the gastric tissue were determined by quantitative polymerase chain reaction(q PCR). Pathological gastric ulcer indexes, levels of pro-inflammatory cytokines(IL-1β, IL-17, TNF-α) and lipid peroxidation were significantly decreased in HI-treated groups, whereas the levels of protective factors(TGF-β, GSH, SOD activity and PGE2) were significantly elevated especially in the group with HI 1.61 g kg^(-1) d^(-1)(P xpression of COx-2 mRNA decreased significantly in HI groups(P < 0.05). The study investigates that holothurian intestines may act as a kind of marine medicine which have protective effect on IDM-induced gastric ulcer, which could be a dietary preventive agent for the prevention of gastric damage.

关键词： holothurian intestine NSAID indomethacin gastric ulcer sucralfate

Soil Nematode Response to Biochar Addition in a Chinese Wheat Field

在线全文

学校读者我要写书评

暂无评论

Pedosphere 2013年第1期23卷 98-103页

作者： ZHANG xiao-Ke li qi liANG Wen-Ju ZHANG Min BAO xue-lian xIE Zu-Bin State Key Laboratory of Forest and Soil Ecology Institute of Applied Ecology Chinese Academy of Sciences University of Chinese Academy of Sciences Jiangsu Biochar Engineering Center State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences

While studies have focused on the use of biochar as soil amendment, little attention has been paid to its effect on soil fauna. The biochar was produced from slow pyrolysis of wheat straw in the present study. Four treatments, no addition (CK) and three rates of biochar addition at 2 400 (B1), 12 000 (B5) and 48 000 kg ha-1 (B20), were investigated to assess the effect of biochar addition to soil on nematode abundance and diversity in a microcosm trial in China. The B5 and B20 application significantly increased the total organic carbon and the C/N ratio. No significant difference in total nematode abundance was found among the treatments. The biochar addition to the soil significantly increased the abundance of fungivores, and decreased that of plant parasites. The diversity of soil nematodes was significantly increased by B1 compared to CK. Nematode trophic groups were more effectively indicative to biochar addition than total abundance.

关键词： diversity ecological indices microcosm trial nematode community trophic groups

Sustained release of Semaphorin 3A from α-tricalcium phosphate based cement composite contributes to osteoblastic differentiation of MC3T3-E1 cells

在线全文

学校读者我要写书评

暂无评论

Frontiers of Materials Science 2015年第3期9卷 282-292+208页

作者： Jin-Ning WANG Bin PI Peng WANG xue-Feng li Hui-lin YANG xue-Song ZHU Department of Orthopaedic Surgery The first Affiliated Hospital of Soochow University

The reinforcement of calcium phosphate materials with silk fibroin(SF)has been one of the strategies to overcome the brittleness. However, the lack of osteoinductivity may still restrict their further use. This study aimed to investigate the biocompatibility and osteogenesis capacity of a novel Semaphorin 3A-loaded chitosan microspheres/SF/α-tricalcium phosphate composite(Sema3A CMs/SF/α-TCP) in ***3 A was first incorporated into CMs, and the Sema3 A CMs/SF/α-TCP composite was then prepared. The morphology of the CMs was observed using SEM. The in vitro release kinetics, cytotoxicity, and cell compatibility were evaluated, and the real-time quantitative polymerase chain reaction(RT-q PCR) and activity of alkaline phosphatase(ALP) were used to evaluate the osteogenesis capacity of the composite. The in vitro release of Sema3 A from the Sema3 A CMs/SF/α-TCP composite showed a temporally controlled manner. The extract of the Sema3 A CMs/SF/α-TCP composite presented no obvious side effect on the MC3T3-E1 cell proliferation, nor promote cell proliferation. The MC3T3-E1 cells were well-spread and presented an elongated shape on the Sema3 A CMs/SF/α-TCP composite surface; the ALP activity and the osteogenic-related gene expression were higher than those seeded on the surface of the CMs/SF/α-TCP and SF/α-TCP *** conclusion, Sema3 A CMs/SF/α-TCP has excellent biocompatibility and contributes to the osteoblastic differentiation of MC3T3-E1 cells.

关键词： α-tricalcium phosphate (α-TCP) silk fibroin (SF) Semaphorin 3A osteoblastic differentiation MC3T3-E1 cell

Effects of ex situ conservation on diversity and function of the gut microbiota of the Tibetan wild ass(Equus kiang)

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Integrative Zoology 2023年第6期18卷 1089-1104页

作者： Hongmei GAO Feng JIANG Jingjie ZHANG xiangwen CHI Pengfei SONG Bin li Zhenyuan CAI Tongzuo ZHANG Key Laboratory of Adaptation and Evolution of Plateau Biota Northwest Institute of Plateau BiologyChinese Academy of SciencesXiningChina qinghai Provincial Key Laboratory of Animal Ecological Genomics XiningChina College of life Sciences University of Chinese Academy of SciencesBeijingChina Department of Student Affairs Qinghai UniversityXiningChina

Ex situ conservation is the main method for the protection of endangered *** explore the effect of ex situ conservation on the gut microbiota of the kiang(Equus kiang),metagenomic sequencing combined with bioinformatics analysis was used to investigate the composition and function of the gut microbiota of the *** results showed that ex situ conservation not only protected wildlife,but also affected the composition and function of gut microbiota,as well as the health of *** the zoo,the ratio of the relative abundance of Firmicutes to that of Bacteroidetes(F/B)is higher,clusters of potentially pathogenic bacteria(such as Catonella,Catonella,and Mycoplasma)are more numerous,the abundance of resistance genes is higher,and the abundance of metabolic functions is *** dynamic changes of the gut microbiota also played an important role in the nutritional absorption,energy metabolism,and environmental adaptation of the *** the rearing environment and increasing food diversity play important roles for increasing the diversity of gut microbiota,reducing the spread of potentially pathogenic bacteria,and reducing *** the wild,especially in winter and in food-deficient areas,food supplementation can enhance the gut microbial homeostasis of wild animals and reduce the impact of *** depth studies of the gut microbial function of wildlife have important implications for improving ex situ conservation.

关键词： ex situ conservation metagenome potential pathogenic bacteria resistance genes Tibetan wild ass

Discovery of a deep, low mass ratio overcontact binary GSC 03517–00663

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research in Astronomy and Astrophysics 2015年第6期15卷 889-895页

作者： Di-Fu Guo Kai li Shao-Ming Hu Yun-Guo Jiang Dong-Yang Gao xu Chen Shandong Provincial Key Laboratory of Optical Astronomy and Solar-Terrestrial Environment Institute of Space Sciences Shandong University Key Laboratory for the Structure and Evolution of Celestial Objects Chinese Academy of Sciences

When observing blazars, we identified a new eclipsing binary named GSC 03517-00663. The light curves of GSC 03517-00663 are typical of EW-type light curves. Based on the observation using the 1 m telescope at the Weihai Observatory of Shandong University, complete VRI light curves were determined. Then, we analyzed the multiple light curves using the Wilson-Devinney program. It was found that GSC 03517-00663 has a mass ratio of q = 0.164 and a degree of contact of f = 69.2%. GSC 03517-00663 is a deep, low mass ratio overcontact binary. The light curves of GSC 03517-00663 show a strong O＇Connell effect, which was explained by employing a dark spot on the secondary component.

关键词： stars binaries close -- stars binaries eclipsing -- stars individual (GSC 03517-00663)

Hetero-nanostructure of silver nanoparticles on MO_x(M=Mo,Ti and Si) and their applications

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 2011年第6期54卷 865-875页

作者： DONG WenJun ZHAO Haixin li ChaoRong MEI Jie CHEN BeYong TANG WeiHua SHI Zhan FENG ShouHua Center for Optoelectronics Materials and Devices Center for Nanoscience and Nanotechnology ' Key Laboratory of Advanced TextileMaterials and Manufacturing Technology Ministry' of Education' Engineering Research Center for Eco-Dying & Finishing q[' Textiles' Zhejian g Sci- Tech UniversiO' Han gzhou 310018 China State Key Laboratory of Inorganic Synthesis and Preparative Chemisto' College of Chemistry Jilin University Changchun 130012 China

This paper reviews the recent research progress in the hetero-nanostructure of silver nanoparticles on MOx (M=Mo, Ti and Si). It describes the general synthesis of hetero-nanostructure of silver nanoparticles on MOx, and then outlines the interesting catalytic and sensing properties found in these hetero-nanostructures of silver nanoparticles on MOx.

关键词： hetero-nanostructure silver nanoparticles MoO3 TiO2 SiO2 catalyst and sensor

应用3.0T MRI评价缺血性脑血管病与鼻窦炎性病变的关系

在线全文

学校读者我要写书评

暂无评论

中国组织工程研究与临床康复 2007年第48期11卷 9722-9725页

作者：李晖刘怀军朱清风杨冀萍张强河北医科大学第二医院医学影像科河北省石家庄市050000

目的:应用3.0TMRI从影像学角度探讨缺血性脑血管病与鼻旁窦炎性病变的关系及鼻旁窦炎性病变的MRI表现。方法:①分组及检测:2006-10/2007-08河北医科大学第二医院收治经临床及影像学证实为缺血性脑血管病患者218例为病例组,健康体检者21... 详细信息

目的:应用3.0TMRI从影像学角度探讨缺血性脑血管病与鼻旁窦炎性病变的关系及鼻旁窦炎性病变的MRI表现。方法:①分组及检测:2006-10/2007-08河北医科大学第二医院收治经临床及影像学证实为缺血性脑血管病患者218例为病例组,健康体检者218例为对照组,应用美国GE公司Signa Excite HD 3.0T高场强MR扫描仪常规行MRI及MRA检查。②评估指标:MRI图像观察以轴位T2-FSE、轴位T1-FLAIR序列为主,辅以轴位T2-FLAIR、矢状位T1-FLAIR序列,观察并记录脑实质MRI表现、鼻旁窦有无炎性病变。MRA图像观察以MIP重组后图像为主,辅以MRA源图像,观察并记录颅内血管异常情况。主要观察指标包括额窦、筛窦、蝶窦、上颌窦受累情况,各窦病变类型(黏膜增厚、炎性积液、黏膜下囊肿)及相应的MRI表现。结果:①病例组所有患者前循环及后循环系统血管均存在不同程度的动脉粥样硬化改变,143例患者脑实质出现缺血性病变;对照组脑实质及脑血管均未见异常改变。②病例组鼻旁窦有病变者123人次(56.4%),上颌窦病变96例,筛窦病变58例,蝶窦病变30例,额窦病变15例;对照组鼻旁窦有病变者54人次(24.8%),上颌窦病变8例,筛窦病变34例,蝶窦病变2例,额窦病变4例。病例组鼻旁窦炎性病变明显较对照组多(χ2=45.281,P=0.000)。相关系数C=0.91。结论:缺血性脑血管病患者鼻旁窦炎性病变发病率高,缺血性脑血管病与鼻旁窦炎性病变间有一定的相关性。

关键词：缺血性脑血管病鼻旁窦炎症磁共振成像