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Expression of structural genes related to anthocyanin biosynthesis of Vitis amurensis

Journal of Forestry Research 2016年第3期27卷 647-657页

作者： quan Zhao Fei He Malcolm J.Reeves qiu-Hong Pan Chang-qing duan Jun Wang Traditional Chinese Medicine Department Jilin Agricultural Science and Technology College Center for Viticulture and Enology College of Food Science & Nutritional Engineering China Agricultural University Faculty of Applied Science Business and ComputingEastern Institute of Technology

This research was designed to assess the changes in anthocyanin content in grape skins of Vitis amurensis and to explore m RNA transcriptions of 11 structural genes（PAL,CHS3, CHI1, F3H2, F30 H, F3050 H, DFR, LDOX, UFGT,OMT and GST） related to anthocyanin biosynthesis during grape berry development, by the use of HPLC-MS/MS and real-time q-PCR analysis. Accumulation of anthocyanins began at veraison, continued throughout the later berry development and reached a peak at maturity. Veraison is the time when the berries turn from green to purple. Expression of PAL, CHI1, and LDOX were up-regulated from 2 to4 weeks after flowering（WAF）, down-regulated from6 WAF to veraison, whereas DFR was up-regulated at8 WAF, and then up-regulated from veraison to ***3, F3050 H, UFGT, GST, and OMT were down-regulated from 2 WAF to veraison, and then up-regulated from veraison to maturity. The transcriptional expressions of the11 structural genes also showed positive correlations with the anthocyanin content from veraison to maturity. Positive correlations were also observed between OMT transcriptional level and the content of methoxyl-anthocyanins, and between F3050 H transcriptional level and the content of delphinidin anthocyanins. F3H2 and F30 H expression was up-regulated at 2 WAF. F3H2 expression was down-regulated from 4 WAF to veraison and then up-regulated again from veraison to maturity. F30 H expression was down-regulated at 4 WAF and then up-regulated again from 6 WAF to maturity. F30 H transcriptional level was correlated positively with the cyanidin anthocyanin concentration from veraison to maturity. These results indicate that the onset of anthocyanin synthesis during berry development coincides with a coordinated increase in the expression of a number of genes in the anthocyanin biosynthetic pathway.

关键词： Anthocyanins Gene expression HPLC-MS/MS Real-time q-PCR Vitis amurensis

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Austenite Grain Growth Behavior of X80 Pipeline Steel in Heating Process

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Journal of Iron and Steel Research International 2010年第3期17卷 62-66页

作者： duan Lin-na WANG Ji-man LIU qing-you SUN Xin-jun CAO Jian-chun Faculty of Materials and Metallurgy Engineering Kunming University of Science and Technology Kunming 650093 Yunnan China Institute for Structural Materials Central Iron and Steel Research Institute Beijing 100081 China

在Through changing soaking temperature and soaking time, austenite grain growth behavior of X80 pipeline steel under different heating conditions was studied. Relationships of average grain size to soaking temperature and time were obtained respectively. The results show that the prior austenite grains grow with the increase of soaking temperature and time. When soaking temperature is lower than 1 180 ℃, austenite grain size and growth rate are small; when it higher than 1 200 ℃, austenite grains grow rapidly and abnormal grain growth appears. For soaking at 1 180 ℃, austenite grain growth rate is initially high and then decreases when soaking time exceeds 1 h.

关键词： soaking temperature soaking time grain growth grain size

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Interface Modulations on The Nanostructured Chains

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电子显微学报 1997年第4期 49-50页

作者： X.H.Yan 1,2 \ L.D.Zhang 1\ Z.P.duan 1,3 \ q.B.Yang 2 ( 1Institute of Solid State Physics, Chinese Academy of Sciences, Hefei, Anhui 230031 2Department of Physics, Xiangtan University, Xiangtan, Hunan 4111054 3Institute of Me

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ＩｎｔｅｒｆａｃｅＭｏｄｕｌａｔｉｏｎｓｏｎＴｈｅＮａｎｏｓｔｒｕｃｔｕｒｅｄＣｈａｉｎｓＸ．Ｈ．Ｙａｎ１，２Ｌ．Ｄ．Ｚｈａｎｇ１Ｚ．Ｐ．Ｄｕａｎ１，３Ｑ．Ｂ．Ｙａｎｇ２（１ＩｎｓｔｉｔｕｔｅｏｆＳｏｌｉｄＳｔａｔｅＰｈｙｓｉｃｓ，ＣｈｉｎｅｓｅＡｃ...

关键词： The Nanostructured Modulations Chains Interface

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Probe Knots and Hopf Insulators with Ultracold Atoms

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Chinese Physics Letters 2018年第1期35卷 36-40页

作者： Dong-Ling Deng Sheng-Tao Wang Kai Sun L.-M.duan Department of Physics University of MichiganAnn ArborMichigan 48109USA Condensed Matter Theory Center and Joint quantum Institute Department of PhysicsUniversity of MarylandCollege ParkMD 20742-4111USA Center for quantum Information IIISTsinghua UniversityBeijing 100084 Department of Physics Harvard UniversityCambridgeMassachusetts 02138USA

Knots and links are fascinating and intricate topological *** influence spans from DNA and molecular chemistry to vortices in superfluid helium,defects in liquid crystals and cosmic strings in the early *** we find that knotted structures also exist in a peculiar class of three-dimensional topological insulators—the Hopf *** particular,we demonstrate that the momentum-space spin textures of Hopf insulators are twisted in a nontrivial way,which implies the presence of various knot and link *** further illustrate that the knots and nontrivial spin textures can be probed via standard time-of-flight images in cold atoms as preimage contours of spin orientations in stereographic *** extracted Hopf invariants,knots,and links are validated to be robust to typical experimental *** work establishes the existence of knotted structures in Hopf insulators,which may have potential applications in spintronics and quantum information processing.

关键词： Superfluid helium

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Auxin biosynthesis by the YUCCA6 flavin monooxygenase gene in woodland strawberry

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Journal of Integrative Plant Biology 2014年第4期56卷 350-363页

作者： Hong Liu Wei-Fa Xie Ling Zhang Victoriano Valpuesta Zheng-Wen Ye qing-Hua Gao Ke duan Forestry and Fruit Tree Research Institute Shanghai Key Laboratory of Protected Horticultural Technology Shanghai Academy of Agricultural Sciences (SAAS) Shanghai Key Laboratory of Agricultural Genetics and Breeding Institute of Biotechnology Department of Molecular Biology and Biochemistry Universidad de Málaga

Auxin has been regarded as the main signal molecule coordinating the growth and ripening of fruits in strawberry, the reference genomic system for Rosaceae. The mechanisms regulating auxin biosynthesis in strawberry are largely elusive. Recently, we demonstrated that two YUCCA genes are involved in flower and fruit development in cultivated strawberry. Here, we show that the woodland strawberry （Fragaria vesca L.） genome harbors nine loci for YUCCA genes and eight of them encode functional proteins. Transcription pattern in different plant organs was different for all eight FvYUCs. Functionality of the FvYUC6 gene was studied in transgenic strawberry over- expressing FvYUC6, which showed typical high-auxin pheno- types. Overexpression of FvYUC6 also delayed flowering and led to complete male sterility in F. vesta. Additionally, specific repression of FvYUC6 expression by RNA interference signifi- cantly inhibited vegetative growth and reduced plant fertility. The development of leaves, roots, flowers, and fruits was greatly affected in FvYUC6-repressed plants. Expression of a subset of auxin-responsive genes was well correlated with the changes of FvYUC6 transcript levels and free indole-3-acetic acid levels in transgenic strawberry. These observations are consistent with an important role of FvYUC6 in auxin synthesis, and support a main role of the gene product in vegetative and reproductive development in woodland strawberry.

关键词： Auxin biosynthesis Fragaria vesta plant development RNAinterference YUCCA genes

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Interfacial forces between silica surfaces measured by atomic force microscopy

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Journal of Environmental Sciences 2009年第1期21卷 30-34页

作者： duan JinmingKey Laboratory of Northwest Water Resource,Environment and Ecology,MOE,Xi’an University of Architecture and Technology,Xi’an 710055,China Key Laboratory of Northwest Water Resource Environment and Ecology MOE Xi'an University of Architecture and Technology Xi'an 710055 China

Colloidal particle stability and some other interfacial phenomena are governed by interfacial force interactions. The two well known forces are van der Waals force and electrostatic force, as documented by the classical Derjaguin, Landau, Verwey, and Overbeek （DLVO） theory. Moreover, advances in modern instrumentation and colloid science suggested that some short-ranged forces or structure forces are important for relevant colloidal systems. The interfacial and/or molecular forces can be measured as a resultant force as function of separation distance by atomic force microscopy （AFM） colloid probe. This article presents a discussion on AFM colloid probe measurement of silica particle and silica wafer surfaces in solutions with some technical notifications in measurement and data convolution mechanisms. The measured forces are then analyzed and discussed based on the ＇constant charge＇ and ＇constant potential＇ models of DLVO theory. The difference between the prediction of DLVO theory and the measured results indicates that there is a strong short-range structure force between the two hydrophilic surfaces, even at extremely low ionic concentration, such as Milli-q water purity solution.

关键词： colloid probe DLVO theory solvation forces interfacial interactions

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高能量饮食雌性运动大鼠脂肪组织瘦素mRNA的表达

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中国组织工程研究与临床康复 2007年第17期11卷 3376-3378页

作者：柯小剑王人卫李擎上海体育学院运动科学系

目的:瘦素作为调节机体能量平衡的因子在生殖方面亦具有重要的调节作用,观察高能量饮食对雌性运动大鼠脂肪组织瘦素分泌的影响。方法:实验于2005-07/12在上海体育学院运动科学实验中心完成。选用32只SD雌性大鼠,随机(抽签法)分为4组,即... 详细信息

目的:瘦素作为调节机体能量平衡的因子在生殖方面亦具有重要的调节作用,观察高能量饮食对雌性运动大鼠脂肪组织瘦素分泌的影响。方法:实验于2005-07/12在上海体育学院运动科学实验中心完成。选用32只SD雌性大鼠,随机(抽签法)分为4组,即正常组、高能量饮食组、运动组、运动+高能量饮食组,每组8只。正常组:常规基础饲料膳食。高能量饮食组:高能量饲料膳食(每200g基础饲料中加入鸡蛋15g,食糖40g,猪油20g,浓鱼肝油10滴)。运动组:采用递增负荷运动。运动+高能量饮食组:采用递增负荷运动,同时膳食采用高能量饲料。各组大鼠膳食平均40g/(d·只)。除对照组外运动组均采用段氏PT-98型进行运动训练,先适应性进行训练5d,以后每周运动6d,休息1d,训练2次/d,上下午各1次,最后1d的晚上加训1次。负荷设定参考Bedford的动物运动负荷标准,1～6周跑台速度分别为15,20,27,32,35,35m/min,40min/次,坡度为0°。所有组均于4周后隔天行阴道脱落细胞学检查,保证大鼠于动情间期处死,各组交叉进行。观察高能量饮食对雌性运动大鼠的体质量、体内脂肪含量及脂肪组织的瘦素mRNA表达的影响。结果:32只大鼠均进入结果分析。①各组大鼠体质量、腹内脂肪质量的比较:经过高能量饲料喂养后,高能量饮食组的体质量、腹腔内脂肪湿质量及单位体质量脂肪明显高于正常组[(323.58±23.88),(281.01±12.67)g;(11.53±2.99),(6.01±2.10)g;(35.35±7.24)×103,(18.62±2.89)×103;P0.05)。②各组大鼠脂肪组织瘦素mRNA表达的比较:运动组与正常组相比脂肪组织中的瘦素mRNA表达水平明显下降(P0.05)。结论:高能量饮食能够改善运动所导致的雌性大鼠瘦素水平的下降,在一定程度上改善其能量平衡状况。

关键词：运动(Exercise) 瘦素体重食品配方

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Role of estrogen receptor β selective agonist in ameliorating portal hypertension in rats with CCl4-induced liver cirrhosis

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World Journal of Gastroenterology 2016年第18期22卷 4484-4500页

作者： Cheng-Gang Zhang Bin Zhang Wen-Sheng Deng Ming duan Wei Chen Zhi-Yong Wu Department of Gastrointestinal Surgery Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Department of General Surgery Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese Medicine

AIM: To investigate the role of diarylpropionitrile(DPN), a selective agonist of estrogen receptor β(ERβ), in liver cirrhosis with portal hypertension(PHT) and isolated hepatic stellate cells(HSCs).METHODS: Female Sprague-Dawley rats were ovariectomized(OVX), and liver cirrhosis with PHT was induced by CCl4 injection. DPN and PHTPP, the selective ERβ agonist and antagonist, were used as drug interventions. Liver fibrosis was assessed by hematoxylin and eosin(HE) and Masson's trichrome staining and by analyzing smooth muscle actin expression. Hemodynamic parameters were determined in vivo using colored microspheres technique. Protein expression and phosphorylation were determined by immunohistochemical staining and Western blot analysis. Messenger RNA levels were analyzed by quantitative real-time polymerase chain reaction(q RT-PCR). Collagengel contraction assay was performed using gel lattices containing HSCs treated with DPN, PHTPP, or Y-27632 prior to ET-1 addition. RESULTS: Treatment with DPN in vivo greatly lowered portal pressure and improved hemodynamic parameters without affecting mean arterial pressure, which was associated with the attenuation of liver fibrosis and intrahepatic vascular resistance(IHVR). In CCl4-treated rat livers, DPN significantly decreased the expression of Rho A and ROCK Ⅱ, and even suppressed ROCK Ⅱ activity. Moreover, DPN remarkedly increased the levels of endothelial nitric oxide synthase(e NOS) and phosphorylated e NOS, and promoted the activities of protein kinase G(PKG), which is an NO effector in the liver. Furthermore, DPN reduced the contractility of activated HSCs in the 3-dimensional stress-relaxed collagen lattices, and decreased the ROCK Ⅱ activity in activated HSCs. Finally, in vivo /in vitro experiments demonstrated that MLC activity was inhibited by ***: For OVX rats with liver cirrhosis, DPN suppressed liver Rho A/ROCK signal, facilitated NO/PKG pathways, and decreased IHVR, giving rise to reduced portal pre

关键词： Portal hypertension Estrogen receptor Rho-kinase signaling Nitric oxide Hepatic stellate cells

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犬尿氨酸通路代谢产物与产后抑郁关联研究

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中南大学学报（医学版） 2018年第7期43卷 725-731页

作者：汪赛赢全承炫谭颖洁闻少华张吉涛段开明中南大学湘雅三医院麻醉科中南大学湘雅医学院麻醉专业2014级长沙410013

目的:研究产后抑郁患者血浆犬尿氨酸(kynurenine,KYN)通路代谢产物变化情况。方法:收集2014年2月1日至2017年12月31日在中南大学湘雅三医院择期行剖宫产手术的产妇726例,采用爱丁堡产后抑郁量表(Edinburgh Postnatal Depression Scale,E... 详细信息

目的:研究产后抑郁患者血浆犬尿氨酸(kynurenine,KYN)通路代谢产物变化情况。方法:收集2014年2月1日至2017年12月31日在中南大学湘雅三医院择期行剖宫产手术的产妇726例,采用爱丁堡产后抑郁量表(Edinburgh Postnatal Depression Scale,EPDS)在产后第42天对产后抑郁(postpartum depression,PPD)的发生情况进行评估,≥13评定为PPD。测定并比较PPD组24例和对照组48例产妇血浆KYN,喹啉酸(quinolinic acid,qUIN)和犬尿喹啉酸(kynurenic acid,KYNA)的血浆浓度。结果:PPD发生率7.99%,PPD产妇相比非PPD产妇产前1 d血浆KYN浓度明显升高(PqUIN浓度明显升高(PqUIN比值明显降低(PqUIN和KYNA浓度变化与PPD的发病密切相关,纠正血浆KYNA/qUIN比例失衡可能有利于防治PPD。

关键词：犬尿氨酸喹啉酸犬尿喹啉酸产后抑郁症

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Sky Blue and Yellow Cluster Light-Emitting Diodes Based on Asymmetric Cu_(4)l_(4) Nanocubes

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Research 2023年第1期2022卷 517-524页

作者： Nan Zhang Lei qu Huan Hu Ran Huo Yushan Meng Chunbo duan Jing Zhang Chunmiao Han Guohua Xie Hui Xu Key Laboratory of Functional Inorganic Material Chemistry(Ministry of Education)and School of Chemistry and Material Science Heilongjiang University74 Xuefu RoadHarbin 150080P.R.China Hubei Key Lab on Organic and Polymeric Optoelectronic Materials Department of ChemistryWuhan UniversityWuhan 430072P.R.China

Controllably optimizing excited-state characteristics is crucial for luminescent nanoclusters but remains a formidable ***,we report an effective"ligand-induced asymmetrization"strategy for constructing thermally activated delayed fluorescence-featured cubic Cu4l4 nanoclusters with asymmetric configurations,named[tBCzDBFDP]_(2)Cu_(4)l_(4) and[PTZDBFDP]_(2)Cu_(4)l_(4).Through changing 3,6-di-tert-butyl-carbazole(tBCz)to phenothiazine(PTZ)with a stronger electron-donating effect,emission color is tuned from greenish blue of[tBCzDBFDP]_(2)Cu_(4)l_(4) to yellow of[PTZDBFDP]_(2)Cu_(4)l_(4),as well as the triplet locally excited state of the former to the triplet charge transfer state of the ***-correlated spectroscopic investigation indicates that in terms of triplet quenching suppression,[tBCzDBFDP]_(2)Cu_(4)l_(4) is superior to[PTZDBFDP]_(2)Cu_(4)l_(4),in accord with the stabilities of their triplet locally excited state and triplet charge transfer *** a consequence,these asymmetric Cu4l4 nanocubes endowed their cluster light-emitting diodes with the external quantum efficiencies beyond 12%for sky blue and 8%for *** results suggest the significance and effectiveness of ligand engineering for optoelectronic nanoclusters.

关键词： excited asymmetric latter

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