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Upconversion nanoscopy revealing surface heterogeneity of tumor-secreted extracellular vesicles

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Light(Science & Applications) 2023年第4期12卷 502-504页

作者： Rui pu Qiuqiang Zhan Centre for Optical and Electromagnetic Research South China Academy of Advanced OptoelectronicsSouth China Normal UniversityGuangzhou 510006China

Super-resolution microscopic imaging employing upconversion nanoparticles is applied to reveal the surface heterogeneity of tumor cell-derived small extracellular vesicles,i.e.,*** number of surface antigens of every extracellular vesicles can be quantified by both the high imaging resolution and stable brightness of upconversion *** method proves its great potential in nanoscale biological studies.

关键词： surface resolution revealing

LOCAL SCOUR AROUND A LARGE-SCALE VERTICAL CIRCULAR CYLINDER DUE TO COMBINED WAVE-CURRENT ACTION

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Journal of Hydrodynamics 2004年第1期16卷 7-16页

作者： ZHAOMing TENGBin LILin-pu StateKeyLaboratoryofCoastalandOffshoreEngineering DalianUniversityofTechnologyDalian116024China

The local scour around a large -scale vertical circular cylinder due tocombined wave-current action was experimentally and numerically studied in the present *** were carried out to research the affect of the wave height, current velocity and thediameter of the cylinder on the scour process. The topography near the cylinder in various casesduring scour was measured. The cylinder diameter affects the distribution of scour and depositionarea. The maximum scour depth increases evidently when the current velocity increases. The maximumscour depth does not increase after the wave height retains a certain value. A numerical model usinga finite element method for simulating the scour process was established. And the numerical resultsagree well with the measured, data. The numerical model is more applicable in the case oflarge-scale cylinders.

关键词： local scour wave current sediment transport circular cylinder

A smart supramolecular phototherapeutics for specific cancer therapy

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Science China Chemistry 2018年第11期61卷 1353-1354页

作者： Kanyi pu School of Chemical and Biomedical Engineering Nanyang Technological University

Phototherapeutics provides opportunities for cancer therapy because of its high spatiotemporal controllability and minimal invasiveness. For example, photodynamic therapeutics,often termed as photosensitizer (PS), can be administered to the patients with subsequent exposure to a specific light that leads to the death of the disease cells via oxidative ***, the efficacy of traditional PS has been limited by several issues including poor tumor specificity, fast renal clearance, and phototoxic side effect on normal tissues [1].

关键词：癌症治疗超分子可控制性治疗学光力学副作用时间

Optimized porous clay heterostructure for removal of acetaldehyde and toluene from indoor air

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Frontiers of Environmental Science & Engineering 2016年第2期10卷 219-228页

作者： pu ZHAO Lizhong ZHU Department of Environmental Science Zhejiang University Hangzhou 310058 China Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control Hangzhou 310058 China

Adsorption is the most widely used technology for the removal of indoor volatile organic compounds （VOCs）. However, existing adsorbent-based technologies are inadequate to meet the regulatory requirement, due to their limited adsorption capacity and efficiency, especially under high relative humidity （RH） conditions. In this study, a series of new porous clay heterostructure （PCH） adsorbents with various ratios ofmicropores to mesopores were synthesized, characterized and tested for the adsorp- tion of acetaldehyde and toluene. Two of them, PCH25 and PCH50, exhibited markedly improved adsorption capabil- ity, especially for hydrophilic acetaldehyde. The improved adsorption was attributed to their large micropore areas and high micropore-to-mesopore volume ratios. The amount of acetaldehyde adsorbed onto PCH25 at equilibrium reached 62.7 mg. g-l, eight times as much as the amount adsorbed onto conventional activated carbon （AC）. Even at a high RH of 80%, PCH25 removed seven and four times more of the acetaldehyde than AC and the unmodified raw PCHs did, respectively. This new PCH optimized for their high adsorption and resistance to humidity has promising applications as a cost-effective adsorbent for indoor air purification.

关键词： porous clay heterostmcture volatile organiccompounds adsorption adsorbent indoor air

Development of optical nanoprobes for molecular imaging of reactive oxygen and nitrogen species

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Nano Research 2018年第10期11卷 5258-5280页

作者： Xu Zhen Kanyi pu School of Chemical and Biomedical Engineering Nanyang Technological University Singapore 637457 Singapore

Reactive oxygen and nitrogen species （RONS） play important roles in cell signal transduction. However, overproduction of RONS is associated with a series of pathological processes and may disrupt cellular homeostasis, causing oxidative and nitrosative stress. Accurate methods to selectively and specifically monitor RONS in living systems are required to further elucidate the biological functions of these species. Optical imaging possesses high sensitivity, high spatiotemporal resolution, and real-time imaging capability. These qualities are advantageous for the detection of RONS in living systems. This review summarizes the development of optical nanoprobes with near-infrared （NIR） fluorescent, upconversion luminescent, chemiluminescent, or photoacoustic signals for molecular imaging of RONS in living systems. In this review, we discuss the design principles and advantages of RONS-responsive activatable nanoprobes, as well as applications of these optical imaging modalities in different disease models.

关键词： optical imaging reactive oxygen andnitrogen species fluorescent upconversion luminescence chemiluminescence photoacoustic imaging

Mineral materials as feasible amendments to stabilize heavy metals in polluted urban soils

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Journal of Environmental Sciences 2011年第4期23卷 607-615页

作者： Mingkui Zhang Jincheng pu Zhejiang Provincial Key Laboratory of Subtropical Soil and Plant Nutrition College of Environmental and Resource Sciences Zhejiang University Hangzhou 310058 China.

Four minerals, agricultural limestone （AL）, rock phosphate （RP）, palygorskite （PG）, and calcium magnesium phosphate （CMP）, were evaluated by means of chemical fractions of heavy metals in soils and concentrations of heavy metals in leachates from columns to determine their ability to stabilize heavy metals in polluted urban soils. Two urban soils （calcareous soil and acidic soil） polluted with cadmium, copper, zinc and lead were selected and amended in the laboratory with the mineral materials） for 12 months. Results indicated that application of the mineral materials reduced exchangeable metals in the sequence of Pb, Cd Cu Zn. The reduction of exchangeable fraction of heavy metals in the soils amended with different mineral materials followed the sequence of CMP, PG AL RP. Reductions of heavy metals leached were based on comparison with cumulative totals of heavy metals eluted through 12 pore volumes from an untreated soil. The reductions of the metals eluted from the calcareous soil amended with the RP, AL, PG and CMP were 1.98%, 38.89%, 64.81% and 75.93% for Cd, 8.51%, 40.42%, 60.64% and 55.32% for Cu, 1.76%, 52.94%, 70.00% and 74.12% for Pb, and 28.42%, 52.74%, 64.38% and 49.66% for Zn. Those from the acidic soil amended with the CMP, PG, AL, and RP were 25.65%, 68.06%, 78.01% and 79.06% for Cd, 26.56%, 49.64%, 43.40% and 34.68% for Cu, 44.44%, 33.32%, 61.11% and 69.44% for Pb, and 18.46%, 43.77%, 41.98% and 40.68% for Zn. The CMP and PG treatments were superior to the AL and RP for stabilizing heavy metals in the polluted urban soils.

关键词： heavy metals polluted soil chemical fraction chemical immobilization mineral materials

A Remark on Partly Dissipative Reaction Diffusion Systems on IR^n

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Acta Mathematicae Applicatae Sinica 2008年第4期24卷 583-588页

作者： Xue-ke pu College of Maths and Physics Chongqing University Chongqing 400044 China The Graduate School of China Academy of Engineering Physics P.O. Box 2101 Beijing 100088 China Institute of Fundamental Sciences Massey University New Zealand

The long time behavior of the solutions of some partly dissipative reaction diffusion systems is studied. We prove the existence of a compact （L^2 × L^2 - H^1 × L^2） attractor for a partly dissipative reaction diffusion system in Rn. This improves a previous result obtained by A. Rodrigues-Bernal and B. Wang concerning the existence of a compact （L^2 × L^2 - L^2 × L^2） attractor for the same system.

关键词： Reaction diffusion equation attractor asymptotic compactness

Predicting protein subchloroplast locations:the 10th anniversary

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Frontiers of Computer Science 2021年第2期15卷 1-11页

作者： Jian SUN pu-Feng DU College of Intelligence and Computing Tianjin UniversityTianjin300350China

Chloroplast is a type of subcellular organelle in green plants and *** is the main subcellular organelle for conducting photosynthetic *** proteins,which localize within the chloroplast,are responsible for the photosynthetic process at molecular *** chloroplast can be further divided into several *** in different compartments are related to different steps in the photosynthetic *** the molecular function of a protein is highly correlated to the exact cellular localization,pinpointing the subchloroplast location of a chloroplast protein is an important step towards the understanding of its role in the photosynthetic *** process for determining protein subchloroplast location is always costly and time ***,computational approaches were developed to predict the protein subchloroplast locations from the primary *** the last decades,more than a dozen studies have tried to predict protein subchloroplast locations with machine learning *** sequence features and various machine learning algorithms have been introduced in this research *** this review,we collected the comprehensive information of all existing studies regarding the prediction of protein subchloroplast *** compare these studies in the aspects of benchmarking datasets,sequence features,machine learning algorithms,predictive performances,and the implementation *** summarized the progress and current status in this special research *** also try to figure out the most possible future works in predicting protein subchloroplast *** hope this review not only list all existing works,but also serve the readers as a useful resource for quickly grasping the big picture of this research *** also hope this review work can be a starting point of future methodology studies regarding the prediction of protein subchloroplast locations.

关键词： subchloroplast locations sequence features performance measures online services machine learning

Nonlinear Schrödinger Approximation for the Electron Euler-Poisson Equation

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Chinese Annals of Mathematics,Series B 2023年第3期44卷 361-378页

作者： Huimin LIU Xueke pu Faculty of Applied Mathematics Shanxi University of Finance and EconomicsTaiyuan030006China School of Mathematics and Information Sciences Guangzhou UniversityGuangzhou510006China

The nonlinear Schr?dinger(NLS for short)equation plays an important role in describing slow modulations in time and space of an underlying spatially and temporarily oscillating wave *** this paper,the authors study the NLS approximation by providing rigorous error estimates in Sobolev spaces for the electron Euler-Poisson equation,an important model to describe Langmuir waves in a *** derive an approximate wave packet-like solution to the evolution equations by the multiscale analysis,then they construct the modified energy functional based on the quadratic terms and use the rotating coordinate transform to obtain uniform estimates of the error between the true and approximate solutions.

关键词： Modulation approximation Nonlinear Schrödinger equation Electron Euler-Poisson equation