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Withania somnifera (Ashwagandha):a Novel Source of L-asparaginase

Journal of Integrative Plant Biology 2009年第2期51卷 201-206页

作者： Vishal P.Oza Shraddha D.Trivedi pritesh P.parmar R.B.Subramanian B.R.D.School of Biosciences Sardar Patel UniversityV.V.Nagar 388 120 (Gujarat)India B.R.D.School of Biosciences Sardar Patel UniversityV.V.Nagar 388120 (Gujarat)India

Different parts of plant species belonging to Solanaceae and Fabaceae families were screened for L-asparaginase enzyme （E.C.3.***.1.1.）. Among 34 plant species screened for L-asparaginase enzyme. Withania somnifera L. was identified as a potential source of the enzyme on the basis of high specific activity of the enzyme. The enzyme was purified and characterized from W. somnifera, a popular medicinal plant in South East Asia and Southern Europe. Purification was carried out by a combination of protein precipitation with ammonium sulfate as well as Sephadex-gel filtration. The purified enzyme is a homodimer, with a molecular mass of 72 ± 0.5 kDa as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresisand size exclusion chromatography. The enzyme has a pH optimum of 8.5 and an optimum temperature of 37 ℃. The Km value for the enzyme is 6.1 × 10^-2 mmol/L. This is the first report for L-asparaginase from W. somnifera, a traditionally used Indian medicinal plant.

关键词： Fabaceae L-asparaginase purification Sotanaceae Withania somnifera.

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Identification of Potential Species Croton bonplandianum, Sedges and Balanitis aegyptiacafor the Application of Phytoremediation

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American Journal of Plant Sciences 2013年第6期4卷 1246-1251页

作者： pritesh parmar Bhaumik Dave Ketankumar Panchal R. B. Subramanian B R D School of Biosciences Sardar Patel University Vallabh Vidya India.

Heavy metal contamination to the environment is a serious problem in the developing countries due to anthropogenic activities, a threat needs to remediate to sustain the life on earth, technology includes use of microorganisms and different plant species. In consideration of biomass, phytoremediation is a very useful techniques above all, can be exploit by identification of hyperaccumulator, which accumulates the heavy metal under metal stress condition. In view of constraints of efficient plant species in present study thirty seven different plant species were screened for the identification of heavy metal accumulators. Croton bonplandianum, sedges and Balanites aegyptiaca amongst the all exhibit superior potential of heavy metal accumulation. This is the first report to unravel the heavy metal accumulation property of three different plant species which can be exploited for the bioremediation of heavy metals.

关键词： Balanitis aegyptiaca Croton bonplandianum Hyperaccumulator Phytoremediation Screening Sedges

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Identification of a SSR marker (TOM-144) linked to Fusarium wilt resistance in Solanum lycopersicum

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American Journal of Molecular Biology 2013年第4期3卷 241-247页

作者： pritesh parmar Ankit Sudhir R. Preethi Bhaumik Dave Ketankumar Panchal Ramalingam B. Subramanian Arvind Patel K. B. Kathiria B. R. D. School of Biosciences Sardar Patel University Vallabh Vidyanagar India Vegetable research station Anand Agricultural University Anand India

With the discovery of molecular markers and marker assisted selection technology, the research has entered into a new era and has made it possible to develop new and more informative PCR-based markers, including SSR, and to further facilitate the use of markers in tomato breeding. The present study is a step to introduce a new SSR marker (TOM-144) which was deduced after evaluation of eight microsatellite loci amongst the twenty-one different tomato cultivars. The marker selected was inherited and segregated in mendelian fashion as demonstrated in successive generation of a cross between parent cvs. H-24 x GT-2.

关键词： Fusarium Wilt Molecular Marker Tomato

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