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Rat model of anal sphincter injury and two approaches for stem cell administration

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World Journal of Stem Cells 2018年第1期10卷 1-14页

作者： Jacobo Trébol Tihomir Georgiev-Hristov luz Vega-Clemente Ignacio García-Gómez Ana Carabias-Orgaz Mariano García-Arranz Damián García-Olmo Department of General and Digestive Tract Surgery University Hospital“La Paz”Madrid 28046Spain Department of General and Digestive Tract Surgery Villalba General HospitalMadrid 28400Spain New Therapies Laboratory Instituto de Investigación Sanitaria-Fundación Jiménez DíazMadrid 28040Spain Hektoen Institute of Medicine ChicagoIL 60612United States Department of Anaesthesiology Complejo Asistencial deávilaávila 05004Spain New Therapies Laboratory Instituto de Investigación Sanitaria-Fundación Jiménez DíazMadrid 28040Spain Department of General and Digestive Tract Surgery Quiron-Salud HospitalsMadrid 28040Spain Department of Surgery Madrid Autonomous UniversityMadrid 28029Spain

AIM To establish a rat model of anal sphincter injury and test different systems to provide stem cells to injured *** Adipose-derived stem cells(ASCs) were isolated from BDIX rats and were transfected with green fluorescent protein(GFp) for cell tracking. Biosutures(sutures covered with ASCs) were prepared with 1.5 × 10~6 GFpASCs, and solutions of 10~6 GFp-ASCs in normal saline were prepared for injection. Anorectal normal anatomy was studied on Wistar and BDIX female rats. Then, we designed an anal sphincter injury model consisting of a 1-cm extra-mucosal miotomy beginning at the anal verge in the anterior middle line. The sphincter lesion was confirmed with conventional histology(hematoxylin and eosin) and immunofluorescence with 4', 6-diamidino-2-phenylindole(commonly known as DApI), GFp and α-actin. Functional effect was assessed with basal anal manometry, prior to and after injury. After sphincter damage, 36 BDIX rats were randomized to three groups for:(1) Cell injection without repair;(2) biosuture repair; and(3) conventional suture repair and cell injection. Functional and safety studies were conducted on all the animals. Rats were sacrificed after 1, 4 or 7 d. Then, histological and immunofluorescence studies were performed on the surgical *** With the described protocol, biosutures had been covered with at least 820000-860000 ASCs, with 100% viability. Our studies demonstrated that some ASCs remained adhered after suture passage through the muscle. Morphological assessment showed that the rat anal anatomy is comparable with human anatomy; two sphincters are present, but the external sphincter is poorly developed. Anal sphincter pressure data showed spontaneous, consistent, rhythmic anal contractions, taking the form of "plateaus" with multiple twitches(peaks) in each pressure wave. These basal contractions were very heterogeneous; their frequency was 0.91-4.17 per min(mean 1.6980, SD 0.57698), their mean duration was 26.67 s and mean numb

关键词： Fecal incontinence Experimental rat model Anal sphincter Cell implantation Cell therapy Stem cells Mesenchymal stem cell

Exploring the adjustment to parasite pressure hypothesis: differences in uropygial gland volume and haemosporidian infection in palearctic and neotropical birds

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Current Zoology 2021年第2期67卷 147-156页

作者： Sergio MAGALLANES Anders pape MøLLER Charlene LUJÁN-VEGA Esteban FONG Daniel VECCO Wendy FLORES-SAAVEDRA luz GARCÍA-LONGORIAA Florentino DE LOpE JoséA.IANNACONE Alfonso MARZAL Department of Anatomy Cellular Biology and ZoologyUniversity of ExtremaduraAvda.de Elvas S/NBadajoz ES-06006Spain Ecologie Systématique Evolution UniversitéParis-SudOrsay 91400France Key Laboratory for Biodiversity Science and Ecological Engineering of Ministry of Education College of Life SciencesBeiing Normal UniversityBeiing 100875China pharmacology and Toxicology Graduate Group University of CaliforniaDavisDavisCA 95616USA EverGreen Institute-San Rafael Distrito de IndianaLoreto 16200Perú Centro Urku de Estudios Amazónicos Tarapoto 22202Perú Universidad Nacional Agraria La Molina Lima 15024Perú Molecular Ecology and Evolution Lab Department of BiologyLund UniversityLund 22100Sweden Laboratorio de Ingenieria Ambiental Universidad Cientifica del Sur-Villa el SalvadorLima 15067Perú Laboratorio de Invertebrados Universidad Ricardo PalmaSantiago de Surco 15039Perú

parasites are globally widespread pathogenic organisms,which impose im portant selective forces upon their ***,in accordance with the A djustm ent to parasite pressure hypothesis,it is expected that defenses among hosts vary relative to the selective pressure imposed by *** to the latitudinal gradient in diversity,species richness and abundance of parasites peak near the *** uropygial gland is an im portant defensive exocrine gland against pathogens in *** of the uropygial gland has been proposed to vary among species of birds because of divergent selection by pathogens on their ***,we should expect that bird species from the tropics should have relatively larger uropygial glands for their body size than species from higher ***,this hypothesis has not yet been ***,we analyze the size of the uropygial gland of 1719 individual birds belonging to 36 bird species from 3 Neotropical(peru)and 3 temperate areas(Spain).Relative uropygial gland volum e was 12.52%larger in bird species from the tropics than from temperate *** finding is consistent w ith the relative size of this defensive organ being driven by selective pressures imposed by *** also explored the potential role of this gland as a means of avoiding haemosporidian infection,showing that species with large uropygial glands for their body size tend to have lower mean prevalence of haemosporidian infection,regardless o f their geographical *** result provides additional support for the assum ption that secretions from the uropygial gland reduce the likelihood of becoming infected with haemosporidians.

关键词： adjustment to parasite pressure hypothesis malaria Neotropical region preen oil temperate region

关于熵数、非紧性的测量和插值的一些注记(英文)

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数学进展 2012年第2期41卷 129-138页

作者： Fernando Cobos luz M.Fernandez-Cabrera 马德里康普顿斯大学数学科学系数学分析教研室马德里康普顿斯大学统计学院应用数学分部

本文综述了关于熵数和非紧性的测量的插值性质,本文也描述了经典结果及几个非常新的结果。

关键词：熵数非紧性的测量实(复)插值方法极限插值方法紧算子

The Role of Mutual Support Groups for the Control of Diabetes in a Mexican City: Achievements and Limitations from the patients’ perspective

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Health 2014年第15期6卷 1984-1993页

作者： luz María Tejada-Tayabas María Judith Rios Lugo Faculty of Nursing Autonomus University of San Luis Potosi San Luis Potosí México

Background: Mutual Support Groups (MSG) provides to the patient, the ability to effective self-management behaviors, such as taking prescribed medications, following diet and exercise regimens, self-monitoring, and coping emotionally with the rigors of living with diabetes. physicians, nurses and health promoters from public primary Health Care Centers (HCC) are responsible for providing follow-up care through MSGs. However, although the MSG program has been carried out, in the last decade Mexico presents the most alarming statistics in the prevalence and complications of Diabetes Mellitus type II (DM-II), suggesting a low impact of MSG in the strategy to support the effective control of the disease. Objetive: The aim of this work was to assess whether knowledge or benefits of effective management to control of DM-II, also to identify strengths and limitations of MSGs, in six different Health Care Centers (HCC), in San Luis potosi, Mexico. This research provides an overview of patients’ perception, and significant issues that demand to adjust MGSs strategies, with the goal of exceed the expectations of current health statistics. Methods: A qualitative evaluation was carried out, with an ethnographic approach design. The study included 28 diabetic persons, (21 women and 7 men from ruraland urban carecenters). Semi-structured interviews, non-participant observation, and structured content analysis were used. Results: Support groups give patients a way to address emotional issues, and learn about their disease and self-care, although some patients consider participation an inconvenient obligation. Support group users mention barriers such as lack of continuity in support group activities, inconvenient meeting times, and the difficulty of commuting to attend group sessions. They also mention that overworked health care workers find it difficult to provide leadership to keep the group going. These issues have multiple implications for the success of strategies to control

关键词： Mutual Support Group Diabetes Mellitus Qualitative Research Health Care Services Mexico

Ant Species Richness Variation Mediated by Seasonality in Two Ant-Acacias from Oaxaca, Mexico

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Open Journal of Ecology 2021年第1期11卷 24-31页

作者： Sandra luz Gómez-Acevedo Javier Martínez-Toledo Unidad de Morfología y Función Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de México Mexico City México

Climatic conditions affect the richness of ants resulting in number variation throughout the year. The ants present in the Neotropical system Acacia-pseudomyrmex have been previously studied considering only one sampling season. In contrast, in this study we analyze the richness in two ant-acacia systems during the rainy and dry seasons. The study was carried out on a plot located in the state of Oaxaca, Mexico where 14 ant species, 3 mutualistic and 11 non-mutualistic were found, of which 2 represent new records for the state. Ant species number differed between ant-acacia species and between seasons. The highest richness was recorded in Acacia hindsii in both rainy and dry seasons, corresponding with a low-reward host. During the dry season, A. hindsii represents a highly valuable resource. This study shows that the neotropical system is much more dynamic than it has been previously considered and offers a valuable opportunity for subsequent ecological studies for a better understanding of this complex system.

关键词： Dolichoderinae Formicinae Myrmicinae pseudomyrmex

三毛作品的英西译本对比研究 ——以《撒哈拉的故事》为例

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三毛作品的英西译本对比研究 ——以《撒哈拉的故事》为例

作者： luz Marina Sanchis Saez 厦门大学

学位级别：硕士

三毛的《撒哈拉的故事》写于20世纪70年代中期,该书在中国非常畅销。本文对该书的英文和西文译本进行了对比分析。原文文本的写作语言为中文,这将丰富双语译本的对比分析。本文首先对翻译理论的历史进行了综述,并对皮姆(pym)和韦努蒂(Ve... 详细信息

三毛的《撒哈拉的故事》写于20世纪70年代中期,该书在中国非常畅销。本文对该书的英文和西文译本进行了对比分析。原文文本的写作语言为中文,这将丰富双语译本的对比分析。本文首先对翻译理论的历史进行了综述,并对皮姆(pym)和韦努蒂(Venuti)的翻译理论进行了重点分析。之后,本文对汉英、汉西文学翻译理论的历史和发展趋势进行了探讨。本文的主体部分为三毛《撒哈拉的故事》的英文和西文译本的对比分析。西文译本标题为Diarios del Sahara,由西班牙译者Irene Tor-Carroggio执笔;英文译本标题为Storoes of the Sahara,由美国译者Canaan Morse执笔。西文译本已于2016年出版,英文译本预计将于2019年出版。尽管如此,纸托邦(paper Republic)在网站发布了该书“哑奴”这一章的的翻译,本研究选取该部分的译文作为分析对象。本对比分析的主要目标有两个:首先是厘清两个译者所选择的翻译策略;其次是比较两个译本翻译策略的异同。三语的对比分析旨在研究两个译者对“异化”、“归化”翻译策略的取舍。对是否有脚注形式的增译信息的研究是笔者选取的一种研究方法。在本研究进行的过程中,西文译员接受了笔者的采访,向笔者介绍了其翻译三毛著作的历程。在语言层面,笔者从并列句和从句,以及词汇范围和修辞语言等层面对两个译本进行分析。在语言层面,笔者对不同的翻译策略进行了对比分析,这对理解中文、英文以及西文之间的语际差异非常重要。笔者在对比分析的其他部分也探讨了译者的写作风格。总而言之,本文对中国文学作品的英译和西译所面临的难点进行了分析,对两位译者解决这些难点所采取的翻译策略进行了探讨。

关键词：三语比较研究异化归化三毛隐喻的翻译

如何国际化可以提高竞争优势？智利公司在中国开展业务的情况

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如何国际化可以提高竞争优势？智利公司在中国开展业务的情况

作者： luz Mariela Galdames Silva 宁波大学

学位级别：硕士

本研究旨在展现国际化的主要方面，试图获得可持续的竞争优势时，需要考虑。像分析波特五力模型，佩泰尔，SWOT必须做定期评估，以调整业务或公司策略，公司与任何中等规模的公司，如何在竞争对手的移动的现实，以及如何在价值链的活动... 详细信息

本研究旨在展现国际化的主要方面，试图获得可持续的竞争优势时，需要考虑。像分析波特五力模型，佩泰尔，SWOT必须做定期评估，以调整业务或公司策略，公司与任何中等规模的公司，如何在竞争对手的移动的现实，以及如何在价值链的活动（关键在国外工作时方面）已经完成。对于这一点，适当的指标发展是根本

关键词：国际化竞争优势基冈模型

Fraying around the edges:negative effects of the invasive Tradescantia zebrina *** Bosse(Commelinaceae)on tree regeneration in the Atlantic Forest under different competitive and environmental conditions

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Journal of plant Ecology 2019年第4期12卷 713-721页

作者： Wagner A.Chiba de Castro Rafael O.Xavier Federico H.L.Garrido Jair H.C.Romero Cleto K.peres Ruberval Cda luz Instituto Latino-Americano de Ciências da Vida e da Natureza Universidade Federal da Integração Latino-Americana(UNILA)Foz do IguaçuPR85867-970Brazil Departmento de Ecologia Universidade de São PauloSão PauloSP05508-090Brazil Departamento de Hidrobiologia Universidade Federal de São CarlosSão CarlosSP13565-905Brazil

Aims Invasive plants modify the structure and functioning of natural en-vironments and threat native plant *** species are often favored by human interference such as the creation of arti-ficial forest *** removal experiments may clarify if invasive plants are detrimental to native plant regeneration and how this is related to other local *** assessed the joint effect of envir-onment and competition with the invasive Tradescantia zebrina on tree species recruitment in an Atlantic Forest *** We carried out the experimental study in the Iguaçu National park,located in southern Brazil,using 30 plots distributed across five invaded sites during 6 *** counted *** leaves and recorded the abundance and height of tree recruits over time under contrasting environmental(forest edge *** interior)and removal(all aboveground biomass,only *** removal,and control)*** analyzed the effects of environment and re-moval treatment using generalized linear mixed *** Findings The invasive species performed better at the forest edge than in the *** higher competitive pressure of *** led to higher mortality and lower height of tree *** removal favored tree recruitment,especially in the forest *** study shows that *** hampers woody species regeneration in tropical Atlantic Forests,especially at the forest edge.

关键词： plant invasion competition forest edge plant recruitment Tradescantia zebrina inch plant removal treatment

Developing Genetic Variability of Quinoa (<i>Chenopodium quinoa</i>Willd.) with Gamma Radiation for Use in Breeding programs

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American Journal of plant Sciences 2013年第2期4卷 349-355页

作者： luz Rayda Gomez-pando Ana Eguiluz-de la Barra Cereal and Native Grain Research program National Agricultural University La Molina Lima Peru

Quinoa (Chenopodium quinoa Willd.) is a staple food produced mainly by small-scale subsistence farmers in peru’s highland. Dry seeds (cv. pasankalla) were irradiated with doses of 150 Gy, 250 Gy and 350 Gy. In the M1 generation, the germination process was delayed with increasing radiation dose;seedling height, root length and leaf development were most reduced at 250 Gy and at 350 Gy, no plants survived. In M2, the maximum spectrum of chlorophyll mutations corresponded to 150 Gy and the maximum frequency to 250 Gy. The chlorine mutation was predominant, followed by xantha. Changes were registered for branch number, pedicel length, plant height, life-cycle duration, stem and foliage colour, and leaf morphology at the two doses, with improvements in plant type. More than one mutation per plant was found, especially at 250 Gy. In M3, the same spectrum of mutations was observed, along with a valuable change in grain colour.

关键词： Chenopodium quinoa Gamma Rays Mutant