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Journal of Integrative Plant Biology 2014年第8期56卷 714-728页

作者： lim Chee liew Mohan B. Singh prem l. bhalla Plant Molecular Biology and Biotechnology laboratory Melbourne School of Land and Environment University of Melbourne

legumes, with their unique ability to fix atmo- spheric nitrogen, play a vital role in ensuring future food security and mitigating the effects of climate change because they use less fossil energy and produce less greenhouse gases compared with N-fertilized systems. Grain legumes are second only to cereal crops as a source of human and animal food, and they contribute approximately one third of the protein consumed by the human population. The productivityof seed crops, such as grain legumes, is dependent on flowering. Despite the genetic variation and importance of flowering in legume production, studies of the molecular pathways that control flowering in legumes are limited. Recent advances in genomics have revealed that legume flowering pathways are divergent from those of such model species as Arabidopsis thaliana. Here, we discuss the current understanding of flowering time regulation in legumes and highlight the unique and conserved features of floral evocation in legumes.

关键词： Flowering legume soybean

Biological Parts for Engineering Abiotic Stress Tolerance in Plants

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BioDesign Research 2022年第1期2022卷 395-435页

作者： Neeta lohani Mohan B.Singh prem.l.bhalla Plant Molecular Biology and Biotechnology laboratory Faculty of Veterinary and Agricultural SciencesThe University of MelbourneParkvilleVIC 3010Australia

It is vital to ramp up crop production dramatically by 2050 due to the increasing global population and demand for ***, with the climate change projections showing that droughts and heatwaves becoming common in much of the globe,there is a severe threat of a sharp decline in crop yields. Thus, developing crop varieties with inbuilt genetic tolerance toenvironmental stresses is urgently needed. Selective breeding based on genetic diversity is not keeping up with the growingdemand for food and feed. However, the emergence of contemporary plant genetic engineering, genome-editing, and syntheticbiology offer precise tools for developing crops that can sustain productivity under stress conditions. Here, we summarize thesystems biology-level understanding of regulatory pathways involved in perception, signalling, and protective processesactivated in response to unfavourable environmental conditions. The potential role of noncoding RNAs in the regulation ofabiotic stress responses has also been highlighted. Further, examples of imparting abiotic stress tolerance by geneticengineering are discussed. Additionally, we provide perspectives on the rational design of abiotic stress tolerance throughsynthetic biology and list various bioparts that can be used to design synthetic gene circuits whose stress-protective functionscan be switched on/off in response to environmental cues.

关键词： protective crops precise

Transcriptome-Based Examination of Putative Pollen Allergens of Rice （Oryza sativa ssp. japonica）

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Molecular Plant 2008年第5期1卷 751-759页

作者： Scott D. Russell prem l. bhalla Mohan B. Singh Department of Botany and Microbiology University of Oklahoma Norman OK 73019 USA Plant Molecular Biology and Biotechnology laboratory Australian Research Council Centre of Excellence for Integrative Legume Research Faculty of Land and Food Resources The University of Melbourne Parkville Vic. 3010 Australia

Pollen allergens are among the most abundantly transcribed and translated products in the life history of plants, and particularly grasses. To identify different pollen allergens in rice, putative allergens were identified in the rice genome and their expression characterized using the Affymetrix 57K rice GeneChip microarray. Among the most abundant pollen-specific candidate transcripts were Ory s 1 beta-expansin, Ory s 2, Ory s 7 EF hand, Q ry s 11, Ory s 12 profilin A, Ory s 23, glycosyl hydrolase family 28 （polygalacturonase）, and FAD binding proteins. Highly expressed pollen proteins are frequently present in multiple copy numbers, sometimes with mirror images located on nearby regions of the opposite DNA strand. Many of these are intronless and inserted as copies that retain nearly exact copies of their regulatory elements. Ory s 23 reflects low variability and high copy number, suggesting recent gene amplification. Some copies contain pseudogenes, which may reflect their origin through activity of retrotransposition; some putative allergenic sequences bear fusion products with repeat sequences of transposable elements （lTRs）. The abundance of nearby repetitive sequences, activation of transposable elements, and high production of mRNA transcripts appear to coincide in pollen and may contribute to a syndrome in which highly transcribed proteins may be copied and inserted with streamlined features for translation, including grouping and removal of introns.

关键词： life history Pollen Introns pollen antigen Transposons Allergens Gene Amplification

A Systems Approach for Analyzing Vegetative and Soil Degradation in Arnigad Micro-watershed of Indian Himalayan Region

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Journal of Mountain Science 2010年第4期7卷 315-326页

作者： Mohit Gera prem l.Sankhayan Ole Hofstad Govt. of J&K VanbhavanBelow GumatJammu-180001(J&K) India Department of Ecology and Natural Resource Management Norwegian University of Life Sciences(UMB)P.O.Box 5003N-1432 sNorway

This study analyzes the vegetative and soil degradation,measured as biomass and soil loss,for Arnigad micro-watershed located in Indian Himalayan state of Uttarakhand,in systems framework by using dynamic linear programming bio-economic *** focus is at investigating the effects of alternate policy regimes,i.e.,introduction of improved energy sources for cooking along with substitution of existing local livestock breeds with improved breed,reduction in human population growth and introduction of high yielding varieties of main crops including paddy,maize and *** model horizon extended over a period of 25 years,i.e.,from 2006 to *** was found that the model scenario incorporating increased use of improved energy sources along with substitution of local cows by improved cows could be the most effective policy option in reducing vegetative and soil *** vegetative biomass density declined to 19.76% compared to 35.24% in the BASE scenario and soil erosion loss was also lowered by 29.13%.Also,the reduction of population growth rate to half of the BASE scenario led to minor improvements in *** of high yielding varieties of main crops slightly increased vegetative degradation but reduced soil loss(8.35%) with respect to the BASE *** a phenomenon could be explained in terms of changed crop mix resulting in reduced amount of crop by-products requiring increased lopping of tree branches for animal *** policy option of the increased use of improved energy sources along with substitution of improved breed of cows resulted in 9.58% higher *** of high yielding varieties of crops led to 1.92% increase in income,but the income decreased by 1.25 % when population growth was reduced to *** usefulness of the model lies in analyzing the systems behavior in its entirety where the results can predict the possible direction of change as a result of manipulation in alternate economic regimes.

关键词： Bio-economic model Dynamic linearprogramming Improved energy sources Vegetativeand soil degradation Soil erosion Indian Himalayanregion

Heavy metal induced DNA changes in aquatic macrophytes: Random amplified polymorphic DNA analysis and identification of sequence characterized amplified region marker

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Journal of Environmental Sciences 2009年第5期21卷 686-690页

作者： Meetu Gupta Neera bhalla Sarin Department of Biosciences Jamia Millia Islamia New Delhi-110025 India School of life Sciences Jawaharlal Nehru University

Plants have been used as good bio-indicators and genetic toxicity of environmental pollution in recent years. In this study, aquatic plants Hydrilla verticillata and Ceratophyllum demersum treated with 10umol/l Cd, 5 umol/l Hg, and 20 umol/l Cu for 96 h, showed changes in chlorophyll, protein content, and in DNA profiles. The changes in DNA profiles included variation in band intensity, presence or absence of certain bands and even appearance of new bands. Genomic template stability test performed for the qualitative measurement of changes in randomly amplified polymorphic DNA （RAPD） profiles, showed significant effect at the given concentration of metals. Cloning and sequencing of bands suggested that these markers although may not be homologous to any known gene but its conversion as a sequence characterized amplified region （SCAR） marker is useful in detecting the effects of genotoxin agents.

关键词： RAPD Hydrilla verticillata Ceratophyllum demersum DNA polymorphism, SCAR marker

Modeling Spatial–Temporal Dynamics of Urban Residential Fire Risk Using a Markov Chain Technique

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International Journal of Disaster Risk Science 2019年第1期10卷 57-73页

作者： Rifan Ardianto prem Chhetri School of Business IT and logistics RMIT (Royal Melbourne Institute of Technology) University

This article applies a Markov chain method to compute the probability of residential fire occurrence based on past fire history. Fitted with the fire incidence data gathered over a period of 10 years in Melbourne, Australia,the spatially-integrated fire risk model predicts the likely occurrence of fire incidents using space and time as key model parameters. The mapped probabilities of fire occurrence across Melbourne show a city-centric spatial pattern where inner-city areas are relatively more vulnerable to a fire than outer suburbia. Fire risk reduces in a neighborhood when there is at least one fire in the last1 month. The results show that the time threshold of reduced fire risk after the fire occurrence is about 2 *** risk increases when there is no fire in the last 1 month within the third-order neighborhood(within 5 km). A fire that occurs within this distance range, however, has no significant effect on reducing fire risk level within the neighborhood. The spatial–temporal dependencies of fire risk provide new empirical evidence useful for fire agencies to effectively plan and implement geo-targeted fire risk interventions and education programs to mitigate potential fire risk in areas where and when they are most needed.

关键词： Australia Markov chain Melbourne Residential fire risk Spatial– temporal analysis

Influence of A-site cation disorder on structural and magnetocaloric properties of Nd_(0.7–x)la_xSr_(0.3)MnO_3(x=0.0, 0.1, 0.2 & 0.3)

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Journal of Rare Earths 2015年第10期33卷 1072-1080页

作者： Sudharshan Vadnala prem Pal Saket Asthana Department of Physics Indian Institute of Technology Hyderabad

In the present research, the influence of A-site ionic size disorder on structural, magnetic and magnetocaloric properties of Ndo.7_xtaxSr0.3MnO3 （for x=0.0, 0.1, 0.2 ＆ 0.3） compounds were investigated. These compounds were synthesized by solid state reaction route and crystallized in orthorhombic structure with Pnma space group. An average ionic radius 〈rA〉 increased with la-substitution which influenced the physical properties such as the magnetic entropy varying from 4.78 J/kg.K （for x-=0.0） to 3.55 J/kg.K （for x=0.3） and the maximum magnetization at 5 K changing from 3.92 μB （for x=0.0） to 3.59 μB （for x=0.3）. The change in maximum magnetic entropy decreased with increase of A-site average ionic radius. The decrease in magnetic entropy indicated the suppression of lattice disorder that influenced the spin-lattice coupling. The ferromagnetic transition temperature shifted toward room temperature with la substitution. As 〈rA〉 increased, the phase transition changed from first order （for x-=0.0） to second order （for x≥ 0.1） and the refrigeration capacity increased to a significant level. The modest change in magnetic entropy over a wide range of transition temperature might be useful for future active magnetic refrigeration technology.

关键词： manganites magnetocaloric effect relative cooling Curie temperature rare earths

EFFECTS OF UNDERSCOUR DEPTH AND HORIZONTAl SPACING BETWEEN TWO BED PROTECTION BlOCKS ON STABIlITY OF FRONTAl BlOCK

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Journal of Hydrodynamics 2012年第4期24卷 579-588页

作者： SHAH prem TANAKA Norio YAGISAWA Junji Graduate School of Science and Engineering Saitama University Sakura-ku Saitama-shi Saitama Japan

The stability of a river-bed protection block in a flood event is affected by underscouring of the block. The effects of block spacing on block stability were investigated in different underscour conditions. Three blocks were placed at the bottom in a flume, and the underscour depth of the frontal block and distance between the first and second blocks were changed. Drag and lift forces were measured directly in each experimental condition. Particle Image Velocimetry （PIV） was used to visualize the flow and eva- luate the streamline pattern. Pressure measurements around the block to understand the pressure distribution show that the pressure distribution on the rear and bottom surfaces of the block is significantly affected by changes in block spacing and underscour depth. The experimental results show that for the same underscour gap, lift, and drag coefficients decreased with increasing space between the two blocks. In addition, if the underscour gap is increased with the same horizontal spacing, the lift coefficient increases and drag coefficient decreases. These results demonstrate that wider spacing enhance stability of the frontal block in the underscour condition. Moreover, analysis of block stability with changing underscouring and horizontal spacing shows that bed protection blocks seem to have more stability when the gap between two bed protection blocks was equal to height of the blocks.

关键词： bed protection block stability underscouring Particle Image Velocimetry (PIV)

Recent advances in catalytic combustion of AP-based composite solid propellants

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Defence Technology（防务技术） 2021年第3期17卷 1013-1031页

作者： Narendra Yadav prem Kumar Srivastava Mohan Varma Department of Space Engineering&Rocketry India Department of Chemistry Birla Institute of TechnologyMesraRanchiIndia

Composite solid propellants(CSPs) have widely been used as main energy source for propelling the rockets in both space and military applications. Internal ballistic parameters of rockets like characteristic exhaust velocity, specific impulse, thrust, burning rate etc., are measured to assess and control the performance of rocket motors. The burn rate of solid propellants has been considered as most vital parameter for design of solid rocket motors to meet specific mission requirements. The burning rate of solid propellants can be tailored by using different constituents, extent of oxidizer loading and its particle size and more commonly by incorporating suitable combustion catalysts. Various metal oxides(MOs),complexes, metal powders and metal alloys have shown positive catalytic behaviour during the combustion of CSPs. These are usually solid-state catalysts that play multiple roles in combustion of CSPs such as reduction in activation energy, enhancement of rate of reaction, modification of sequences in reaction-phase, influence on condensed-phase combustion and participation in combustion process in gas-phase reactions. The application of nanoscale catalysts in CSPs has increased considerably in recent past due to their superior catalytic properties as compared to their bulk-sized counterparts. A large surface-to-volume ratio and quantum size effect of nanocatalysts are considered to be plausible reasons for improving the combustion characteristics of propellants. Several efforts have been made to produce nanoscale combustion catalysts for advanced propellant formulations to improve their energetics. The work done so far is largely scattered. In this review, an effort has been made to introduce various combustion catalysts having at least a metallic entity. Recent developments of nanoscale combustion catalysts with their specific merits are discussed. The combustion chemistry of a typical CSP is briefly discussed for providing a better understanding on role of co

关键词： Composite solid propellants Burn rate modifier Metallic nano-catalysts Catalytic combustion Thermal decomposition