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Effect of Biosynthesized Silver Nanoparticles on Staphylococcus aureus Biofilm Quenching and Prevention of Biofilm Formation

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Nano-Micro Letters 2012年第1期4卷 34-39页

作者： pratik r.chaudhari Shalaka A.Masurkar Vrishali B.Shidore Suresh P.Kamble Center for Biotechnology Pravara Institute of Medical Sciences

The development of green experimental processes for the synthesis of nanoparticles is a need in the field of nanotechnology. The synthesis of silver nanoparticles was achieved using Bacillus cereus supernatant and1 m M silver nitrate. 100 m M glucose was found to quicken the rate of reaction of silver nanoparticles ***-visible spectrophotometric analysis was carried out to assess the synthesis of silver nanoparticles. The synthesized silver nanoparticles were further characterized by using Nanoparticle Tracking Analyzer(NTA),Transmission Electron Microscope and Energy Dispersive X-ray spectra. These silver nanoparticles showed enhanced quorum quenching activity against Staphylococcus aureus biofilm and prevention of biofilm formation which can be seen under inverted microscope(40 X). The synergistic effect of silver nanoparticles along with antibiotics in biofilm quenching was found to be effective. In the near future, silver nanoparticles could be used in the treatment of infections caused by highly antibiotic resistant biofilm.

关键词： Silver Nanoparticles Green Synthesis Bacillus cereus Biofilm Quorum Quenching

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rapid Biosynthesis of Silver Nanoparticles Using Cymbopogan Citratus (Lemongrass) and its Antimicrobial Activity

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Nano-Micro Letters 2011年第3期3卷 189-194页

作者： Shalaka A.Masurkar pratik r.chaudhari Vrishali B.Shidore Suresh P.Kamble Pravara Institute of Medical Sciences

The present study deals with the rapid green synthesis of silver nanoparticles using fresh leaves of Cymbopogan Citratus(Lemongrass). Silver nanoparticles were formed within 8～10 minutes by microwave irradiation using aqueous solution of Ag NO3(1 m M) with fresh leaves extract of Cymbopogan Citratus. The synthesized silver nanoparticles were characterized by using UV-visible spectrophotometer analysis, nanoparticle tracking analyzer, transmission electron microscope and energy dispersive X-ray spectra. The antibacterial activity of these nanoparticles was studied against multiple drug resistant hospital isolates of ***, ***,*** and hospital isolates of S. typhi, ***. Also, the antifungal activity of these nanoparticles was studied against ***(hospital isolate) and ***(NCIM 616). The synergistic effect of silver nanoparticles along with antibiotics was also studied against multiple drug resistant hospital isolates and found to be effective. The extracellular synthesis of Silver nanoparticles using leaves of Cymbopogan Citratus appears to be rapid and eco-friendly.

关键词： Green synthesis Cymbopogan Citratus Nanoparticle tracking analyzer Transmission electron microscope Energy dispersive X-ray spectra

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