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Expression Pattern of Sucrose Transporters in Arabidopsis thalianaDuring Aphid (Myzus persicae) Infestation

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American Journal of Plant Sciences 2013年第12期4卷 47-51页

作者： Neeraj kumar Dubey Asif Idris Ashok kumar Verma Krishnappa Chandrashekar Kapil Deo Pandey CSIR—National Botanical Research Institute Rana Pratap Marg Lucknow India Department of Botany Banaras Hindu University Varanasi India

Herbivorous insects change the metabolism of the plant during their attack. Our study reports the changes in the expression pattern of sucrose transporters in response to the infestation of aphids at different time intervals. Results showed a significant enhancement in the expression pattern for six out of nine sucrose transporters in response to aphid infestation, followed by suppression after some point. During an earlier time point of infestation, the expressions of sucrose transporters were enhanced probably to compensate for the energy requirements of the damaged cell. However, suppression of sucrose transporters at a later stage may be a defense strategy of the plant to repel the aphids because at a later stage of infestation, aphids become a secondary sink. To complement our assumption, we performed aphid infestation choice and reproductive performance tests in the null mutant of one of the transporters, SUC2, which was compromised in phloem loading of sucrose. Results showed that the mutant was less preferable to aphid for choice as well as reproduction performance.

关键词： Sap Sucking Insects Aphid Sucrose Transporters (SUCs)

Variations in Solar Cycles 22, 23 &24 and Their Effect on Earth’s Climate

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International Journal of Astronomy and Astrophysics 2016年第1期6卷 8-13页

作者： Prithvi Raj Singh Chandra Mani Tiwari Ajay kumar Saxena Department of Physics Awadhesh Pratap Singh University Rewa India

In the present work, the study of influence of solar activity, cosmic ray intensity and geomagnetic activity on Earth’s climate during solar cycles 22, 23 and 24 has been done. The change in Earth’s climate, specifically the change in the global mean temperature has been associated with the variation of some solar activity indices, cosmic ray intensity and geomagnetic activity indices in the period of 1986-2014 (Till Dec.). The important solar indices that are total solar irradiance (TSI), Sunspot Number (SSN), F10.7 index, Cosmic Ray Intensity (CRI) Kiel (NM), geomagnetic activity indices Auroral Electrojet Index (AE) and aa index, have been presented. The study of the Earth’s climate in relationship with solar activity, cosmic ray intensity and geomagnetic activity has been analysed with variations and correlations. The variations of SSN with CRI are in anti-phase;SSN with F10.7, SSN with TSI, SSN with AE, SSN with aa are in same phase. The correlation of SSN with CRI is strongly negatively correlated;SSN with F10.7, SSN with TSI is strongly positively and SSN with AE, SSN with aa positively correlated to averaging solar cycles 22, 23 and 24. The Earth’s climate will be affected by the solar activity, cosmic ray intensity and geomagnetic activity.

关键词： Solar Activity Geomagnetic Activity Cosmic Ray Intensity

Distinct Role of Core Promoter Architecture in Regulation of Light-Mediated Responses in Plant Genes

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Molecular Plant 2014年第4期7卷 626-641页

作者： Rakesh Srivastava Krishan Mohan Rai Meenal Srivastava Verandra kumar Bindu Pandey Sudhir P. Singh Sumit K. Bag Brahma Deo Singh Rakesh Tuli Samir V. Sawant Plant Molecular Biology and Genetic Engineering Division CSIR-National Botanical Research Institute Rana Pratap Marg Lucknow-226001 (U.R) India School of Biotechnology Faculty of Science Banaras Hindu University Varanasi India National Agri-Food Biotechnology Institute (DBT) C-127 Industrial Area Phase VIII Mohali India Academy of Scientific & Innovative Research New Delhi India

In the present study, we selected four distinct classes of light-regulated promoters. The light-regulated promoters can be distinctly grouped into either TATA-box-containing or TATA-less （initiator-containing） promoters. Further, using either native promoters or their swapped versions of core promoter elements, we established that TATA-box and Inr （Initiator） elements have distinct mechanisms which are involved in light-mediated regulation, and these elements are not swappable. We identified that mutations in either functional TATA-box or Inr elements lead to the formation of nucleosomal structure. The nucleotide diversity in either the TATA-box or Inr element in Arabidopsis ecotypes proposes that the nucleotide variation in core promoters can alter the gene expression. We show that motif overrepresentation in light-activated promoters encompasses different specific regulatory motifs present downstream of TSS （transcription start site）, and this might serve as a key factor in regulating light promoters which are parallel with these elements. Finally, we conclude that the TATA-box or Inr element does not act in isolation, but our results clearly suggests the probable involvement of other distinct core promoter elements in concurrence with the TATA-box or Inr element to impart selectivity to light-mediated transcription.

关键词： TATA-box initiator element nucleosome cis-regulatory elements nucleotide polymorphism light transcription.

Spray Prediction Model for Aonla Rust Disease Using Machine Learning

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Journal of Agricultural Science and Technology(B) 2023年第1期13卷 1-12页

作者： Hemant kumar Singh Bhanu pratap S.K.Maheshwari Ayushi Gupta Anuradha Chug Amit Prakash Singh Dinesh Singh Acharya Narendra Deva University of Agriculture and Technology Ayodhya 224229U.P.India ICAR-Central Institute for Arid Horticulture Bikaner 334006RajasthanIndia University School of Information Communication&TechnologyGGSIPUNew Delhi 110012India ICAR-Indian Agricultural Research Institute New Delhi 110012India

Disease prediction in plants has acquired much attention in recent *** factors such as:temperature,relative humidity,rainfall,sunshine play an important role in a plan’s growth only if they are present in adequate amounts as required by the *** the other hand,if the factors are inadequate,they may also support the growth of a disease in the *** current study focuses on the Rust disease in Aonla fruits and leaves by utilizing a real time dataset of weather *** different models are tested for spray prediction on conducive *** resampling techniques,random over sampling(ROS)and synthetic minority oversampling technique(SMOTE)have been used to balance the dataset and five different classifiers:support vector machine(SVM),logistic regression(LR),k-nearest neighbor(kNN),decision tree(DT)and random forest(RF)have been used to classify a particular day based on weather conditions as conducive or *** classifiers are then evaluated based on four performance metrics:accuracy,precision,recall and *** results indicate that for imbalanced dataset,kNN is appropriate with high precision and recall *** both balanced and imbalanced dataset models,the proposed model SMOTE-RF performs best among all models with 94.6%accuracy and can be used in a real time application for spray ***,timely fungicide spray prediction without over spraying will help in better productivity and will prevent the yield loss due to rust disease in Aonla crop.

关键词： Aonla Internet of Things machine learning plant disease rust spray prediction.

Comparative Study of Different Materials with Al-Sic for Engine Valve Guide by Using FEM

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World Journal of Engineering and Technology 2016年第2期4卷 238-251页

作者： Hemendra kumar Srivastva Arurendra Singh Chauhan Manmohan Kushwaha Amaan Raza Prashantkr Bhardwaj Vaibhav Raj Department of Mechanical Engineering Naraina Group of Institutions Kanpur India Department of Mechanical Engineering Maharana Pratap Engineering College Kanpur India

In this work, an effort has been designed to raise the reliability of engine using Al-Sic composites with other alternatively materials for the engine valve guides. Aluminum matrix composites have found the most suitable inside automotive, aerospace and aircraft industries and contain the greatest promise for future year’s growth. The finite element analysis of the Al-Sic composite with Titanium alloy (Ti-834), Copper Nickel Silicon alloys (CuNi3Si), and aluminum bronze alloy as an alternative material for engine valve guide was done using Ansys 13.0 software. The stress analysis of engine valve guide under the different pressure and temperature is considered, the pressure is taken as from 10 MPa to 100 MPa with different temperatures varying from 600℃ to 650℃. The temperature, principal stress and principal strain distribution on the entire surface area of the engine valve guide were obtained. The stresses were observed to be well below the permitted stress for all the materials but the Al-Sic composites found the most suitable one. Valve guide is modeled in pro-engineer software and analysis is carried out in Ansys 13.0. The deformations and stresses induced due to structural and thermal loading is illustrated and discussed.

关键词： Valve Guide Composite Materials FEM Deformation Von Mises Stress

Impact of obesity and diabetes on arthritis: An update

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Health 2013年第1期5卷 143-156页

作者： Rajesh Pandey Narendra kumar Seema Paroha Ram Prasad Mukesh Yadav Shalini Jain Hariom Yadav Amity Institute of Microbial Technology Amity University Noida India College of Advance Studies Datia India Department of Biochemistry Awadhesh Pratap Singh University Rewa India Department of Biochemistry Jawaharlal Nehru Agriculture University Jabalpur India Department of Biotechnology IMS Engineering College Ghaziabad India NIDDK National Institutes of Health Bethesda USA

The incidence of obesity and diabetes has been increased with alarming rate in recent years and became a common problem around the globe including developing as well as in developed countries with incalculable social costs. Obesity and type 2 diabetes are two common co-morbidities occur together. Obesity and diabetes is closely associated with many diseases, osteoarthritis, hypertension, certain form of cancer, sleep-breathing disorders and coronary heart disease. Impacts of obesity and diabetes (insulin resistance) on arthritis have been seen in patients that we associated with combination of various factors like increased availability of high-energy foods, genetic susceptibility and decreased physical activity in modern society. Arthritis is becoming pandemic around the globe and its occurrence with obesity and diabetes has been observed more common than ever. Combination of these two chronic conditions makes these diseases more vulnerable for human health. Till now very limited information is established about the pathological and mechanistic correlation among these health ailments. In this review article we aimed to survey the literature covering the influence of obesity and diabetes on arthritis pathology and tried to establish correlation with these diseases.

关键词： Obesity Diabetes Arthritis Complications

Characterization of winged bean (Psophocarpus tetragonolobus(L.) DC.) based on molecular, chemical and physiological parameters

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American Journal of Molecular Biology 2013年第4期3卷 187-197页

作者： Chandra Sekhar Mohanty Sushma Verma Vinayak Singh Shahina Khan Priyanka Gaur Priya Gupta M. Abdul Nizar Nilamani Dikshit Rojalin Pattanayak Alpika Shukla Abhishekh Niranjan Nayan Sahu Soumit kumar Behera Tikam Singh Rana CSIR-National Botanical Research Institute Rana Pratap Marg Lucknow Uttar Pradesh India National Bureau of Plant Genetic Resources Regional Station Akola Maharashtra India Sanjay Gandhi Institute of Post-Graduate Medical Sciences Lucknow Uttar Pradesh India University of Lucknow Lucknow Uttar Pradesh India

Winged bean (Psophocarpus tetragonolobus (L.) DC.) is a potential legume crop of the tropics with high protein and oil content in the seeds. Analysis of the mutual genotypic relationships among twenty four genotypes of P. tetragonolobus through Mantel test found a significant correlation (r = 0.839) between similarity matrices of the results obtained from the use of the RAPD and ISSR molecular markers. The UPGMA tree based on Jaccard’s similarity coefficient generated from their cumulative data showed two distinct clusters and seven sub-clusters among these accessions. Quantification of total polyphenols, flavonoids and tannin revealed the highest percentage of occurrence of kaempferol (1.07-790.5 μg/g) and the lowest percentage of gallic acid (0.09-3.49 μg/g) in the seeds. Phytochemical analysis of the winged bean genotypes revealed that, some of the exotic lines are distinct. Analysis of photosynthesis rate, photosynthetic yield and stomatal conductance data also showed two clusters and was in congruence with the phytochemical affinities of the genotypes. The overall high level of polymorphism and varied range of genetic distances across the genotypes revealed a wide range of genetic base of P. tetragonolobus. The present investigation therefore, has provided significant insights for further improvement of winged bean germplasm for its qualitative and quantitative traits.

关键词： Psophocarpus tetragonolobus Polyphenols Flavonoid RAPD ISSR Physiological Parameter