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Optimized protoplast isolation and transfection with a breakpoint:accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot

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aBIOTECH 2024年第2期5卷 151-168页

作者： Debasmita Panda Subhasis Karmakar Manaswini Dash Swagat kumar Tripathy Priya Das Sagar Banerjee Yiping Qi Sanghamitra Samantaray pradipta kumar Mohapatra Mirza J.Baig Kutubuddin A.Molla ICAR National Rice Research Institute CuttackOdisha 753006India Department of Botany Ravenshaw UniversityCuttackOdisha 753003India Department of Plant Science and Landscape Architecture University of MarylandCollege ParkMD 20742USA

The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target genomic ***-predicted guide RNAs(gRNAs)often fail to induce efficient cleavage at target *** target loci are inaccessible due to complex chromatin ***,there is no suitable tool available to predict the architecture of genomic target sites and their ***,significant time and resources are spent on performing editing experiments with inefficient *** in vitro-cleavage assay could provide a rough assessment of gRNA efficiency,it largely excludes the interference of native genomic *** in-vivo testing gives a proper assessment of the cleavage ability of editing reagents in a native genomic ***,we developed a modified protocol that offers highly efficient protoplast isolation from rice,Arabidopsis,and chickpea,using a sucrose gradient,transfection using PEG(polyethylene glycol),and validation of single guide RNAs(sgRNAs)cleavage efficiency of *** have optimized various parameters for PEG-mediated protoplast transfection and achieved high transfection efficiency using our protocol in both monocots and *** introduced plasmid vectors containing Cas9 and sgRNAs targeting genes in rice,Arabidopsis,and chickpea *** dual sgRNAs,our CRISPRdeletion strategy offers straightforward detection of genome editing success by simple agarose gel *** sequencing of PCR products confirmed the editing efficiency of specific ***,we demonstrated that isolated protoplasts can be stored for up to 24/48 h with little loss of viability,allowing a pause between isolation and *** high-efficiency protocol for protoplast isolation and transfection enables rapid(less than 7 days)validation of sgRNA cleavage efficiency before proceeding with stable *** isolation and transfection method can als

关键词： Protoplast Genome editing Transgene-free edited plants Rice Chickpea

Determination of the time of refractive stability after uneventful phacoemulsification in Indian eyes

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World Journal of Experimental Medicine 2024年第2期14卷 89-96页

作者： Ashok kumar nanda Bijnya Birajita Panda Asish Swain Logesh Balakrishnan Department of Ophthalmology Kar Vision Eye HospitalBhubaneswar 751013OdishaIndia Department of Ophthalmology All India Institute of Medical SciencesBhubaneswar 751019OdishaIndia Department of Optometry Kar Vision Eye HospitalBhubaneswar 751013OdishaIndia Department of Statistics Apollo HospitalsChennai 823104India

BACKGROUND Knowledge about refractive stabilization and the accuracy of postoperative refractive error measurements are crucial for improved patient outcomes after *** guidelines typically recommend waiting 4-6 wk before prescribing corrective *** research focused on identifying factors that influence refractive errors in the early stages of post-cataract surgery,thus contributing to the existing literature on this *** To investigate the time required for refraction stability after uneventful phacoemulsification *** We compared the variation and statistical significance of the difference in spherical,cylindrical components,and the spherical equivalent between the 1-and 6-wk follow-up period in a group of 257 eyes that underwent uneventful phacoemulsification with foldable intraocular lens implantation,all performed by a single experienced *** Wilcoxon-Signed Rank Test was utilized to assess the magnitude of the change and determine its statistical *** refractive stability was defined as the point at which the change in spherical equivalent was within±0.5 dioptres for two consecutive *** The average age of the patients was 64.9±8.9 *** differences observed in both the visits in spherical power(0.1±0.2),cylinder power(0.3±0.4),and spherical equivalent(0.2±0.2)were minimal and not statistically *** majority of eyes(93.4%)achieved refractive stability within 6 wk after the *** cylindrical power differed between age groups at the 6th wk post-operative and the difference was statistically significant(P value 0.013).There were no significant differences in refractive stability when considering sex and axial *** Phacoemulsification with foldable intraocular lens implantation results in no significant changes in refraction for the majority of cases during the 6-wk follow-up ***,a spectacle prescription can be given at the completion of 1 wk.

关键词： Cataract surgery Phacoemulsification Refraction stability Visual acuity Spherical equivalent

Enhanced Energy Efficient with a Trust Aware in MANET for Real-Time Applications

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Computers, Materials & Continua 2023年第4期75卷 587-607页

作者： M.V.Narayana Vadla Pradeep kumar Ashok kumar nanda Hanumantha Rao Jalla Subba Reddy Chavva Department of CSE Guru Nanak Institutions Technical CampusHyderabadRanga Reddy DistrictTelangana501506India Department of CSE B V Raju Institute of TechnologyNarsapurMedakTelangana502313India Department of CSE T.R.R.Government Degree College KandukurKandukurA.P.523105India Department of IT VFST&Research(Deemed to be University)VadlamudiA.P.522213India

Mobile ad hoc networks (MANETs) are subjected to attack detectionfor transmitting and creating new messages or existing message *** attacker on another node evaluates the forging activity in themessage directly or indirectly. Every node sends short packets in a MANETenvironment with its identifier, location on the map, and time through *** attackers on the network broadcast the warning message usingfaked coordinates, providing the appearance of a network collision. Similarly,MANET degrades the channel utilization performance. Performancehighly affects network performance through security algorithms. This paperdeveloped a trust management technique called Enhanced Beacon TrustManagement with Hybrid Optimization (EBTM-Hyopt) for efficient clusterhead selection and malicious node detection. It tries to build trust amongconnected nodes and may improve security by requiring every participatingnode to develop and distribute genuine, accurate, and trustworthy materialacross the network. Specifically, optimized cluster head election is done periodicallyto reduce and balance the energy consumption to improve the lifetimenetwork. The cluster head election optimization is based on hybridizingParticle Swarm Optimization (PSO) and Gravitational Search OptimizationAlgorithm (GSOA) concepts to enable and ensure reliable routing. Simulationresults show that the proposed EBTM-HYOPT outperforms the state-of-thearttrust model in terms of 297.99 kbps of throughput, 46.34% of PDR, 13%of energy consumption, 165.6 kbps of packet loss, 67.49% of end-to-end delay,and 16.34% of packet length.

关键词： MANET malicious nodes clustering trust management beacon message

Multi-scale analysis for transport of fine settling particles through an ice-covered channel in a laminar flow condition

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International Journal of Sediment Research 2022年第6期37卷 794-808页

作者： Subham Dhar nanda Poddar B.S.Mazumder Kajal kumar Mondal Department of Mathematics Cooch Behar Panchanan Barma University Physics and Applied Mathematics Unit Indian Statistical Institute Department of Civil Engineering Indian Institute of Technology Bombay

The current research deals with the dispersion of fine settling particles in a fluid flowing through an icecovered channel under the laminar flow condition. An analytical solution of the two-dimensional convection-diffusion equation, based on the multi-scale homogenization technique, is obtained. To validate the current study, analytical results for the dispersion coefficient are compared with the available earlier research work. Moreover, the proposed analytical solutions for mean concentration distributions of the tracers are compared with the numerical results obtained from the finite difference technique. From the industrial and environmental points of view, the vertical concentration distribution provides a very significant information. The downstream evolution of the concentration distribution also is shown for typical time periods at different values of the settling velocity. The approach to the vertical uniformity shows that it is too slow a process in comparison to that of longitudinal normality. It was found that settling velocities of particles disturb the vertical uniformity and the centroid of the solute cloud rises due to the increase in settling velocity. Results illustrate that in the downstream direction, the vertical concentration distribution increases near the bed surface and it decreases in the proximity of the ice-covered surface of the channel with the increase of settling velocity, but the mean concentration of the solute increases. The current study may play an important role to understand the mechanism of the sedimentation process in a closed channel system.

关键词： Sediment transport Dispersion Ice-covered channel Vertical uniformity Multi-scale analysis

Short-term mixed electricity demand and price forecasting using adaptive autoregressive moving average and functional link neural network

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Journal of Modern Power Systems and Clean Energy 2019年第5期7卷 1241-1255页

作者： Sujit kumar DASH pradipta Kishore DASH Siksha O Anusandhan Deemed to be University Bhubaneswar 751030India

A new hybrid adaptive autoregressive moving average(ARMA)and functional link neural network(FLNN)trained by adaptive cubature Kalman filter(ACKF)is presented in this paper for forecasting day-ahead mixed short-term demand and electricity prices in smart *** hybrid forecasting framework is intended to capture the dynamic interaction between the electricity consumers and the forecasted prices resulting in the shift of demand curve in electricity *** proposed model comprises a linear ARMA-FLNN obtained by using a nonlinear expansion of the weighted *** nonlinear functional block helps introduce nonlinearity by expanding the input space to higher dimensional space through basis *** train the ARMA-FLNN,an ACKF is used to obtain faster convergence and higher forecasting *** proposed method is tested on several electricity markets,and the performance metrics such as the mean average percentage error(MAPE)and error variance are compared with other forecasting methods,indicating the improved accuracy of the approach and its suitability for a real-time forecasting.

关键词： Mixed demand and price forecasting Cubature Kalman filter Price spikes Smart grids

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Hybrid IWD-DE:A Novel Approach to Model Cooperative Navigation Planning for Multi-robot in Unknown Dynamic Environment

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Journal of Bionic Engineering 2019年第2期16卷 235-252页

作者： Degal Chandrasekhar Rao Manas Ranjan Kabat pradipta kumar Das Prabir kumar Jena Department of Information Technology VSSUTBurlaOdisha 768018India Department of Computer Science&Engineerings VSSUT、Burla Odisha 76801&India Department of Mechanical Engineering VSSUTBurlaOdisha 76801&India

The current study emphasizes inadequacies of earlier research findings on multi-robot navigation and offers a robust approach to guide the robots in an unknown dynamic *** attain the objective,the Intelligent Water Drop(IWD)algorithm is combined with Differential Evolution(DE)in a new conceptual fashion to optimize the navigation path of multiple mobile *** hybrid IWD-DE,basic IWD and DE algorithms are then explored for multi-robot navigation in a dynamic workspace through *** simulation outcomes demonstrate the competency of the proposed method to guide the robot in predicting the nature of the obstacle and to generate an optimal and safe trajectory in a dynamic *** potency of the proposed algorithm is further validated through experimentation with fire bird robot in a real *** outcomes of the simulation and experimentation,by employing IWD-DE algorithm are compared with those of basic IWD and DE *** error estimated for Average Navigation Path Travelled(ANPT),Aggregate Navigation Path Deviation(ANPD)and number of turns between simulation and experimental results by employing IWD-DE is 8.96,9.09,and 11.13,respectively while reaching 16.43,28.61,29.15 for IWD,and 6.66,10.20,14.28 for *** efficiency of the proposed approach is further verified with the state-of-the-art in a dynamic *** outcomes reveal the efficiency and flexibility of the proposed approach in resolving the multi-robot navigation problem as compared to the state-of-the-art in a dynamic workspace.

关键词： multi-robot dynamic environment intelligent water drop differential evolution navigation planning waypoints fire bird robot

A state-of-the-art review of solar passive building system for heating or cooling purpose

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Frontiers in Energy 2016年第3期10卷 347-354页

作者： Arun kumar nanda C K PANIGRAHI Birla Institute of Technology Mesra Ranchiranchi 835215 India School of Electrical Engineering Kalinga Institute of Industrial Technology Bhubaneswar 751024 India

The major portion of energy in a building is consumed by heating, ventilating, and air-conditioning （HVAC）. The traditional heating and cooling systems contribute greatly to the emission of greenhouse gases, especially carbon dioxide. Four different ways, i.e., Trombe wall, solar chimney, unglazed transpired solar facade, and solar roof, are adopted for solar heating. Similarly, two major ways, i.e., evaporative cooling and building integrated evaporative cooling are adopted for cooling of the building. Therefore, an attempt has been made in this paper to compile the developments of solar heating and cooling technologies in a building.

关键词： HVAC heating cooling solar heating carbondioxide (CO2) emissions

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DLMNN Based Heart Disease Prediction with PD-SS Optimization Algorithm

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Intelligent Automation & Soft Computing 2023年第2期35卷 1353-1368页

作者： S.Raghavendra Vasudev Parvati R.Manjula Ashok kumar nanda Ruby Singh D.Lakshmi S.Velmurugan Department of Information and Communication Technology Manipal Institute of TechnologyManipal Academy of Higher EducationManipal576104India Department of Information Science and Engineering SDM College of Engineering and TechnologyDharwad580002India Department of Computer Science and Engineering School of ComputingSRM Institute of Science and TechnologyKattankulathurChennai603203India Department of Computer Science and Engineering B.V Raju Institute of TechnologyTelangana502313India Faculty of Engineering and Technology SRM Institute of Science and TechnologyNCR CampusUttar Prasesh201204India School of Computing Science and Engineering VIT Bhopal UniversityMadhya Pradesh466114India Department of Computer Science and Engineering Vel Tech Multi Tech Dr.Rangarajan Dr.Sakunthala Engineering CollegeChennai600062India

In contemporary medicine,cardiovascular disease is a major public health *** diseases are one of the leading causes of death *** are classiﬁed as vascular,ischemic,or *** information contained in patients’Electronic Health Records(EHR)enables clin-icians to identify and monitor heart *** failure rates have risen drama-tically in recent years as a result of changes in modern *** diseases are becoming more prevalent in today’s medical *** year,a substantial number of people die as a result of cardiac *** primary cause of these deaths is the improper use of pharmaceuticals without the supervision of a physician and the late detection of *** improve the efﬁciency of the classiﬁcation algo-rithms,we construct a data pre-processing stage using feature ***-ments using unidirectional and bidirectional neural network models found that a Deep Learning Modiﬁed Neural Network(DLMNN)model combined with the Pet Dog-Smell Sensing(PD-SS)algorithm predicted the highest classiﬁcation performance on the UCI Machine Learning Heart Disease *** DLMNN-based PDSS achieved an accuracy of 94.21%,an F-score of 92.38%,a recall of 94.62%,and a precision of 93.86%.These results are competitive and promising for a heart disease *** demonstrated that a DLMNN framework based on deep models may be used to solve the categorization problem for an unbalanced heart disease *** proposed approach can result in exceptionally accurate models that can be utilized to analyze and diagnose clinical real-world data.

关键词： Machine learning random forest coronary heart disease cardiovascular optimization algorithm

Comparing accuracy of cochlear measurements on magnetic resonance imaging and computed tomography:A step towards radiation-free cochlear implantation

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中华耳科学杂志:英文版 2023年第4期18卷 208-213页

作者： Anurita Swarup Vinusree Karakkandy Preetam Chappity Suprava Naik Sanjay kumar Behera pradipta kumar Parida Mohnish Grover Gaurav Gupta Prajna Paramita Giri Saurav Sarkar Pradeep Pradhan Dillip kumar Samal Aswathi Kallyadan Veetil Asutosh Adhikari Saurav Nayak Department of ENT and HNS All India Institute of Medical Sciences(AIIMS)BhubaneswarOdishaIndia Department of Radiodiagnosis All India Institute of Medical Sciences(AIIMS)BhubaneswarOdishaIndia Department of Community and Family Medicine All India Institute of Medical Sciences(AIIMS)BhubaneswarOdishaIndia Department of Biochemistry All India Institute of Medical Sciences(AIIMS)BhubaneswarOdishaIndia Department of ENT Sawai Man Singh Medical CollegeJaipurRajasthanIndia Department of ENT Sardar Patel Medical CollegeBikanerRajasthanIndia

Objective:Computed Tomography(CT)and Magnetic Resonance Imaging(MRI)are commonly employed in pre-operative evaluation for cochlear implant ***,with a decrease in the age of implantation,even minor radiation exposure can cause detrimental effects on children over their *** current study compares different cochlear measurements from CT and MRI scans and evaluates the feasibility of using only an MRI scan for radiological evaluation before cochlear ***:A longitudinal observational study was conducted on 94 ears/47 children,employing CT and MRI *** CT and MRI scan measurements include,A value,B value,Cochlear duct length(CDL),twoturn cochlear length,alpha and beta angles to look for cochlear *** nerve diameter was measured using *** values were ***:The mean difference between measurements from CT and MRI scans for A value,B value,CDL,and two-turn cochlear length values was 0.567±0.413 mm,0.406±0.368 mm,2.365±1.675 mm,and 2.063±1.477 mm respectively without any significant *** alpha and beta angle measures were comparable,with no statistically significant ***:The study suggests that MRI scans can be the only radiological investigation needed with no radiation risk and reduces the cost of cochlear implant program in the paediatric *** is no significant difference between the measurements obtained from CT and MRI ***,observed discrepancies in cochlear measurements across different populations require regionally or race-specific standardized values to ensure accurate diagnosis and precision in cochlear implant *** aspect must be addressed to ensure positive outcomes for patients.

关键词： Cochlear implant Hearing loss Cochlea anatomy Radiology

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