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Insights on the crosstalk between dendritic cells and helper T cells in novel genetic etiology for mendelian susceptible mycobacterial disease

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Cellular & Molecular Immunology 2018年第12期15卷 1091-1094页

作者： Emma Rey-jurado Magdalena S.pizarro-Ortega Alexis M.Kalergis Millennium Institute on Immunology and Immunotherapy Departamento de Genética Molecular y MicrobiologíaFacultad de Ciencias BiológicasPontificia Universidad Católica de ChileSantiago 8330644Chile Departamento de Endocrinología Facultad de MedicinaPontificia Universidad Católica de ChileSantiago 8330644Chile

Mendelian susceptibility to mycobacterial disease(MSMD)is an inherited predisposition to infections by Bacille-Calmette Guérin(BCG)vaccine or by environmental *** etiology of MSMD has been associated with up to nineteen different genetic mutations in interferon(IFN)-γ-related genes.1 Although mycobacteria susceptibility-associated genetic mutations are rare in the population,their diagnosis is crucial for an efficient and timely *** et al.2 have recently described an autosomal recessive deficiency in the signal peptidase-like 2 A(SPPL2-a)as a new genetic etiology for MSMD in three patients that had suffered BCG dissemination disease.

关键词： mendelian susceptibility to mycobacterial disease SPPL2a deficiency cDC2 mycobacterial-specific priming IFN-γ

Experimental bio-artificial liver:Importance of the architectural design on ammonia detoxification performance

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World journal of Hepatology 2018年第10期10卷 719-730页

作者： María Dolores pizarro María Eugenia Mamprin Lucas Damián Daurelio joaquín Valentín Rodriguez María Gabriela Mediavilla Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET) Rosario S2002 LRKArgentina Laboratorio de Investigaciones en Fisiología y Biología Molecular Vegetal(LIFiBVe) Facultad de Ciencias AgrariasUniversidad Nacional del LitoralEsperanza 3080Argentina Facultad de Ciencias Bioquímicas y Farmacéuticas Universidad Nacional de RosarioRosario S2002 LRKArgentina Centro Binacional de Criobiología Clínica y Aplicada(CAIC) Universidad Nacional de RosarioRosario S2011 BXNArgentina Instituto de Biología Molecular y Celular de Rosario(IBR CONICET-UNR)Consejo Nacional de Investigaciones Científicas y Tecnológicasy Universidad Nacional de RosarioRosario S2002 LRKArgentina

AIM To determine the influence of the construction design over the biological component's performance in an experimental bio-artificial liver(BAL) *** Two BAL models for liver microorgans(LMOs) were constructed. First, we constructed a cylindrical BAL and tested it without the biological component to establish its correct functioning. Samples of blood and biological compartment(BC) fluid were taken after 0, 60, and 120 min of perfusion. Osmolality, hematocrit, ammonia and glucose concentrations, lactate dehydrogenase(LDH) release(as a LMO viability parameter), and oxygen consumption and ammonia metabolizing capacity(as LMO functionality parameters) were determined. CPSI and OTC gene expression and function were measured. The second BAL, a "flat bottom" model, was constructed using a 25 cm2 culture flask while maintaining all other components between the models. The BC of both BALs had the same capacity(approximately 50 cm3) and both were manipulated with the same perfusion system. The performances of the two BALs were compared to show the influence of *** The cylindrical BAL showed a good exchange of fluids and metabolites between blood and the BC, reflected by the matching of osmolalities, and glucose and ammonia concentration ratios after 120 min of perfusion. No hemoconcentration was detected, the hematocrit levels remained stable during the whole study, and the minimal percentage of hemolysis(0.65% ± 0.10%) observed was due to the action of the peristaltic pump. When LMOs were used as biological component of this BAL they showed similar values to the ones obtained in a Normothermic Reoxygenation System(NRS) for almost all the parameters assayed. After 120 min, the results obtained were: LDH release(%): 14.7 ± 3.1 in the BAL and 15.5 ± 3.2 in the NRS(n = 6); oxygen consumption(μmol/min?g wet tissue): 1.16 ± 0.21 in the BAL and 0.84 ± 0.15 in the NRS(n = 6); relative expression of Cps1 and Otc: 0.63 ± 0.12 and 0.67 ± 0.20, respectively,

关键词： Bio-artificial liver Ammonia detoxification Device design Ornithine Transcarbamylase Rat liver microorgans Carbamyl Phosphate SynthetaseⅠ

Chemical Profile of the Sulphated Saponins from the Starfish Luidia senegalensis Collected as by-Catch Fauna in Brazilian Coast

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Natural Products and Bioprospecting 2018年第2期8卷 83-89页

作者： Marcelo Marucci Pereira Tangerina julia pizarro Cesario Gerson Rodrigues Raggi Pereira Tania Marcia Costa Wagner Cotroni Valenti Wagner Vilegas UNESP-Univ Estadual Paulista Coastal campus of Sao VicentePraca Infante Dom Henrique s/nSao VicenteSP 11330-900Brazil Chemistry Institute Sao Paulo State University-UNESPAraraquara CampusProf.Francisco Degni55SPAraraquara 14800-060Brazil

The by-catch fauna of the shrimp fishery includes a number of marine invertebrates that are discarded because they do not have commercial *** order to try to add some value to these materials,we analyzed the chemical composition of the starfish Luidia senegalensis collected in the Brazilian coast as a consequence of the trawling fishery *** order to access their chemical composition,we used a combination of solid phase extraction(SPE)followed by ultra-high performance liquid chromatography coupled to electrospray ionization ion trap tandem mass spectrometry(UPLC-ESI-IT-MSn).Luidia sene-galensis contains asterosaponins,which are sulphated glycosilated steroids,containing five and six sugar moieties,in addition to *** study helped us to support the presence of important and potentially bioactive compounds in invertebrates associated to the by-catch fauna of the shrimp fishery,using a fast and efficient method.

关键词： Luidia senegalensis By-catch fauna Asterosaponins UPLC-MS

Performance of cold-preserved rat liver Microorgans as the biological component of a simplified prototype model of bioartificial liver

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World journal of Hepatology 2016年第33期8卷 1442-1451页

作者： María Dolores pizarro María Gabriela Mediavilla Alejandra Beatriz Quintana ángel Luis Scandizzi joaquín Valentín Rodriguez María Eugenia Mamprin Centro Binacional de Criobiología Clínica y Aplicada Instituto de Biología Molecular y Celular de Rosario Consejo Nacional de Investigaciones Científicas y Técnicas Morfología Facultad de Ciencias Bioquímicas y Farmacéuticas área Farma-cología Facultad de Ciencias Bioquímicas y FarmacéuticasUniversidad Nacional de Rosario

AIMTo develop a simplified bioartificial liver (BAL) device prototype, suitable to use freshly and preserved liver Microorgans (LMOs) as biological component. METHODSThe system consists of 140 capillary fibers through which goat blood is pumped. The evolution of hematocrit, plasma and extra-fiber fluid osmolality was evaluated without any biological component, to characterize the prototype. LMOs were cut and cold stored 48 h in BG35 and ViaSpan® solutions. Fresh LMOs were used as controls. After preservation, LMOs were loaded into the BAL and an ammonia overload was added. To assess LMOs viability and functionality, samples were taken to determine lactate dehydrogenase (LDH) release and ammonia detoxification capacity. RESULTSThe concentrations of ammonia and glucose, and the fluids osmolalities were matched after the first hour of perfusion, showing a proper exchange between blood and the biological compartment in the minibioreactor. After 120 min of perfusion, LMOs cold preserved in BG35 and ViaSpan® were able to detoxify 52.9% ± 6.5% and 53.6% ± 6.0%, respectively, of the initial ammonia overload. No significant differences were found with Controls (49.3% ± 8.8%, P ® cold preserved LMOs, respectively (n = 6, P CONCLUSIONThis prototype relied on a simple design and excellent performance. It’s a practical tool to evaluate the detoxification ability of LMOs subjected to different preservation protocols.

关键词： Rat liver Microorgans Cold preservation BG35 preservation solution Bioartificial liver device Acute liver failure

Phylogenomic analysis of 2556 single-copy protein-coding genes resolves most evolutionary relationships for the major clades in the most diverse group of lichen-forming fungi

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Fungal Diversity 2018年第5期92卷 31-41页

作者： David pizarro Pradeep K.Divakar Felix Grewe Steven D.Leavitt jen-Pan Huang Francesco Dal Grande Imke Schmitt Mats Wedin Ana Crespo H.Thorsten Lumbsch Departamento de Farmacologı́a Farmacognosia y BotánicaFacultad de FarmaciaUniversidad Complutense de Madrid28040 MadridSpain Science&Education The Field Museum1400 S.Lake Shore DriveChicagoILUSA Department of Biology and M.L.Bean Life Science Museum Brigham Young UniversityLife Science Building 4102ProvoUT 84602USA Senckenberg Biodiversity and Climate Research Centre(SBiK-F) Senckenberganlage 2560325 Frankfurt am MainGermany Institute of Ecology Evolution and DiversityGoethe UniversityFrankfurt am MainMax-von-Laue-Str.960438 Frankfurt am MainGermany Department of Botany Swedish Museum of Natural HistoryPO Box 5000710405 StockholmSweden

Phylogenomic datasets continue to enhance our understanding of evolutionary relationships in many lineages of ***,genome-scale data have not been widely implemented in reconstructing relationships in lichenized *** we generate a data set comprised of 2556 single-copy protein-coding genes to reconstruct previously unresolved rela-tionships in the most diverse family of lichen-forming fungi,*** sampling included 51 taxa,mainly from the subfamily Parmelioideae,and represented six of the seven previously identified major clades within the *** results provided strong support for the monophyly of each of these major clades and most backbone relationships in the topology were recovered with high nodal support based on concatenated dataset and species tree *** alectorioid clade was strongly supported as sister-group to all remaining clades,which were divided into two major *** the first major clade the anzioid and usneoid clades formed a strongly supported sister-group relationship with the cetrarioid?hypogymnioid *** sister-group relationship of Evernia with the cetrarioid clade was also strongly supported,whereas that between the anzioid and usneoid clades needs further *** the second major clade Oropogon and Platismatia were sister to the parmelioid group,while the position of Omphalora was not fully *** study demonstrates the power of genome-scale data sets to resolve long-standing,ambiguous phylogenetic rela-tionships of lichen-forming ***,the topology inferred in this study will provide a valuable framework for better understanding diversification in the most diverse lineage of lichen-forming fungi,Parmeliaceae.

关键词： Fungi Lecanorales Lichenized fungi Parmeliaceae Parmelioideae Phylogeny Systematics

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菲律宾马斯巴特第一个工业堆浸场

黄金 1989年第3期10卷 56-58页

作者： R.S.pizarro 候健

简介安特拉斯联合矿业公司(ACMDC)早在1980年就在马斯巴特金矿进行黄金生产,这个金矿有一个露天矿和一个生产3500吨/日的炭浆厂,矿石储量总计2400万吨,平均金品位1.1克/吨。为了进一步确定大型堆浸场的操作参数,建设了一座400吨的半... 详细信息

简介安特拉斯联合矿业公司(ACMDC)早在1980年就在马斯巴特金矿进行黄金生产,这个金矿有一个露天矿和一个生产3500吨/日的炭浆厂,矿石储量总计2400万吨,平均金品位1.1克/吨。为了进一步确定大型堆浸场的操作参数,建设了一座400吨的半工业试验场并投产。根据这些试验数据,菲律宾设计了一个大型堆浸场。由于矿石的品位低,已作了各种努力来降低成本和操作费用,利用本公司生产铜中闲置的矿山设备来破碎矿石。

关键词：工业堆浸场黄金

Development of Upstream Data-Input Models to Estimate Downstream Peak Flow in Two Mediterranean River Basins of Chile

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Open journal of Modern Hydrology 2014年第4期4卷 132-143页

作者： Roberto pizarro-Tapia Rodrigo Valdés-Pineda Claudio Olivares Patricio A. González University of Talca Technological Center of Environmental Hydrology (CTHA) Talca Chile University of Arizona Department of Hydrology and Water Resources Tucson USA Ministry of Public Works Water Resources Directorate (DGA) Coyhaique Chile

Accurate flood prediction is an important tool for risk management and hydraulic works design on a watershed scale. The objective of this study was to calibrate and validate 24 linear and non-linear regression models, using only upstream data to estimate real-time downstream flooding. Four critical downstream estimation points in the Mataquito and Maule river basins located in central Chile were selected to estimate peak flows using data from one, two, or three upstream stations. More than one thousand paper-based storm hydrographs were manually analyzed for rainfall events that occurred between 1999 and 2006, in order to determine the best models for predicting downstream peak flow. The Peak Flow Index (IQP) (defined as the quotient between upstream and downstream data) and the Transit Times (TT) between upstream and downstream points were also obtained and analyzed for each river basin. The Coefficients of Determination (R2), the Standard Error of the Estimate (SEE), and the Bland-Altman test (ACBA) were used to calibrate and validate the best selected model at each basin. Despite the high variability observed in peak flow data, the developed models were able to accurately estimate downstream peak flows using only upstream flow data.

关键词： Peak Flows Storm Events Flood Forecasting Peak Flow Index Peak Flow Transit Time Linear and No-Linear Models

SIRT3-mediated inhibition of FOS through histone H3 deacetylation prevents cardiac fibrosis and inflammation

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Signal Transduction and Targeted Therapy 2020年第1期5卷 2322-2331页

作者： Xavier Palomer MSilvia Román-Azcona javier pizarro-Delgado Ana Planavila Francesc Villarroya Brenda Valenzuela-Alcaraz Fátima Crispi Álvaro Sepúlveda-Martínez Irene Miguel-Escalada jorge Ferrer jFrancisco Nistal Raquel García Mercy MDavidson Emma Barroso Manuel Vázquez-Carrera Department of Pharmacology Toxicology and Therapeutic ChemistryIBUB(Institut de Biomedicina de la Universitat de Barcelona)Research Institute-Pediatric Research InstituteHospital Sant Joan de Déuand CIBER de Diabetes y Enfermedades Metabólicas Asociadas(CIBERDEM)Faculty of Pharmacy and Food SciencesUniversity of BarcelonaBarcelonaSpain Department of Biochemistry and Molecular Biomedicine IBUB and CIBER Fisiopatología de la Obesidad y Nutrición(CIBEROBN)Faculty of BiologyUniversity of BarcelonaBarcelonaSpain aBCNatal-Barcelona Center for Maternal–Fetal and Neonatal Medicine(Hospital Clínic and Hospital Sant joan de Deu) Institut d’Investigacions Biomèdiques August Pi i SunyerUniversitat de Barcelonaand Center for Biomedical Research on Rare Diseases(CIBER-ER)BarcelonaSpain Genomic Programming of Betacells Laboratory Institut d’Investigacions August Pi i Sunyer(IDIBAPS)and CIBERDEMBarcelonaSpain Section of Epigenomics and Disease Department of Medicineand National Institute for Health Research(NIHR)Imperial Biomedical Research CentreImperial College LondonLondonUK Servicio de Cirugía Cardiovascular Hospital Universitario Marqués de ValdecillaDepartamento de Ciencias Médicas y QuirúrgicasFacultad de MedicinaUniversidad de CantabriaInstituto de Investigación Marqués de Valdecilla(IDIVAL)Centro de Investigación Biomédica en Red Cardiovascular(CIBERCV)Instituto de Salud Carlos IIISantanderSpain Departamento de Fisiología y Farmacología Facultad de MedicinaUniversidad de CantabriaInstituto de Investigación Marqués de Valdecilla(IDIVAL)SantanderSpain Department of Radiation Oncology Columbia UniversityNew YorkNYUSA

Sirtuin 3(SIRT3)is a deacetylase that modulates proteins that control metabolism and protects against oxidative *** of SIRT3 activity has been proposed as a promising therapeutic target for ameliorating metabolic diseases and associated cardiac *** this study,we investigated the role of SIRT3 in inflammation and fibrosis in the heart using male mice with constitutive and systemic deletion of SIRT3 and human cardiac AC16 ***3 knockout mice showed cardiac fibrosis and inflammation that was characterized by augmented transcriptional activity of *** with this,SIRT3 overexpression in human and neonatal rat cardiomyocytes partially prevented the inflammatory and profibrotic response induced by TNF-α.Notably,these effects were associated with a decrease in the mRNA and protein levels of FOS and the DNA-binding activity of ***,we demonstrated that SIRT3 inhibits FOS transcription through specific histone H3 lysine K27 deacetylation at its *** findings highlight an important function of SIRT3 in mediating the often intricate profibrotic and proinflammatory responses of cardiac cells through the modulation of the FOS/AP-1 *** fibrosis and inflammation are crucial in the progression of cardiac hypertrophy,heart failure,and diabetic cardiomyopathy,our results point to SIRT3 as a potential target for treating these diseases.

关键词： SIRT3 inflammation cardiac

口服葡萄糖——电解质溶液治疗242例新生儿腹泻脱水

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新乡医学院学报 1985年第S1期 65-67页

作者： pizarro D 贾汝贤

242例新生儿平均脱水占体重的5.1%,其中234例(96.7%)用葡萄糖——电解质溶液,在平均时间7.44小时内进行口服补液,有8例新生儿(3.3%)需要静脉补液。呕吐不是完成口服补液的障碍,住院时高钠血症、低钠血症、酸中毒,在几小时内被纠正。口... 详细信息

242例新生儿平均脱水占体重的5.1%,其中234例(96.7%)用葡萄糖——电解质溶液,在平均时间7.44小时内进行口服补液,有8例新生儿(3.3%)需要静脉补液。呕吐不是完成口服补液的障碍,住院时高钠血症、低钠血症、酸中毒,在几小时内被纠正。口服补液对没有并发症的新生儿脱水是一种完全满意的首选的治疗方法。

关键词：口服补液并发症低钠血症治疗方法并发病疾病新生儿腹泻电解质溶液水-电解质代谢紊乱