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The Seed Water Sorption Isotherm and Antioxidant-Defensive Mechanisms of <i>Hordeum vulgare</i>L. Primed Seeds

American Journal of Plant Sciences 2018年第12期9卷 2385-2407页

作者： Pinpinatt Junhaeng pitipong thobunluepop Wanchai Chanprasert Damrongvudhi Onwimol Sutkhet Nakasathien Elke Pawelzik Department of Agronomy Faculty of Agriculture Kasetsart University Bangkok Thailand Division of Quality of Plant Products Department of Crop Sciences Faculty of Agricultural Sciences Georg-August-Universit&auml t Gö ttingen G&ouml ttingen Germany

The key successful for seed priming technique was the management of seed imbibition process. This study was aimed to indicate the seed moisture sorption characteristics of Hordeum vulgare L. cv. Samerng 2 in relation to the seed priming technique and its effect on the seed qualities. The experiment was designed by Completely Randomized Design (CRD) with four replications. Seed hydro-priming technique was done under 25 degree Celsius, and 40% - 60% RH. Barley seed moisture content was determined every hour after imbibition for 24 hours. Then, primed barley seed qualities were evaluated at 6, 8, 10, 12, 14, and 16 hours of hydrotime. The results revealed that barley seeds were imbibed rapidly from 10.55% to 37.69% seed moisture content during 0 - 9 hours of imbibition. The constantly seed moisture content was continued until 17 hours of imbibition. Radicle was protruded with 42.74% seed moisture content. Seed moisture sorption characteristic equation was Y = 0.0055x3- 0.267x2 + 4.5931x + 13.348. Coefficient of determination (R2) was 0.916. Primed seed germination was not affected by hydrotime. Primed seeds were improved in speed of germination and seedling growth rate. The best primed barley seed vigorous was noted at 14 - 16 hours of hydrotime. In addition, catalase activity (CAT) and superoxide dismutase activity (SOD) were not significantly different for non-primed and 14 - 16 hours primed seeds. However, lipoxygenase activity (LOX) was increased and malondialdehyde (MDA) content was decreased for 14 - 16 hours primed seeds. Subsequently, 14 - 16 hours primed barley seeds can maintain high seed germination and vigor for 8 - 12 months under 5 degree Celsius. Thus, it could be concluded that hydro-priming for 14 - 16 hours enhances barley seed quality by reducing germination time, induced defenses mechanism, and could be stored for 8 - 12 months.

关键词： Seed Moisture Sorption Characteristic Barley Seed Hydro-Priming Seed Germination Speed of Germination Storability Defenses Mechanism

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Effect of seed priming with different concentrations of potassium nitrate on the pattern of seed imbibition and germination of rice(Oryza sativa L.)

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Journal of Integrative Agriculture 2017年第3期16卷 605-613页

作者： Anisa Ruttanaruangboworn Wanchai Chanprasert pitipong Tobunluepop Damrongvudhi Onwimol Department of Agronomy Faculty of Agriculture Kasetsart University Bangkok 10900 Thailand

Low germination and vigor of rice seed associated with dry-seed broadcasting are common problems encountered by rice *** objectives of this study were to evaluate the role of potassium nitrate（KNO3）on the pattern of seed imbibition and to determine the effect of seed priming with KNO3 on the germination percentage,speed and uniformity of germination in rice *** 1 compared the patterns of seed imbibition of six concentrations of KNO3（0,0.25,0.50,1.00,1.50,and 2.00%）in two rice cultivars-KDML105 and *** results showed that soaking rice seed in KNO3 at higher concentrations could delay the imbibition *** higher concentrations of KNO3 delayed the imbibition time of rice seed and took a longer time to reach the end of phases 1 and 2 compared to the lower *** patterns of seed imbibition using distilled water of both rice cultivars（KDML105 and RD15）were quite similar,but with different concentrations of KNO3,the imbibition time taken to reach the end of phases 1 and 2 was slightly postponed in KDML105 suggesting that different rice cultivars may need different imbibition times for soaking seed in the priming *** 2 evaluated the effects of seed priming with 1.0 and 2.0%KNO3 at different imbibition *** was found that priming with 1.0%KNO3 showed better seed germination than priming with 2.0%KNO3 and seed priming with 1.0%KNO3 at the imbibition time of early phase 2（or 28 h for KDML105）improved seed germination and increased both the speed and uniformity of seed *** results of this study show promise for the use of priming with 1.0%KNO3 soaked until early phase 2 of seed imbibition for improving the seed germination and vigor of rice in dry seed broadcasting.

关键词： rice water uptake seed priming KNO3 solution germination percentage speed of germination uniformity of germination

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