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The occ.r.ence,inheritance,and segregation of complex genomic structural variation in synthetic Brassica napus

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The crop Journal 2024年第2期12卷 515-528页

作者： Dandan Hu Jin Lu Wenwen Li Yinghui Yang Junxiong Xu Han Qin Hao Wang Yan Niu Huaiqi Zhang Qingqing Liu Xiangxiang He Annaliese S.Mason Jc.r.s pires Zhiyong Xiong Jun Zou National Key Laboratory of crop Genetic Improvement College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070HubeiChina college of Agronomy Henan Agricultural UniversityZhengzhou 450002HenanChina Key Laboratory of Herbage and Endemic crop Biotechnology Inner Mongolia UniversityHohhot 010070Inner MongoliaChina Plant Breeding Department University of BonnBonn 53115Germany Department of Soil and crop Sciences Colorado State UniversityFort CollinsCO 80523USA

"Synthetic"allopolyploids recreated by interspecific hybridization play an important role in providing novel genomic variation for crop *** synthetic allopolyploids often undergo rapid genomic structural variation(SV).However,how such SV arises,is inherited and fixed,and how it affects important traits,has rarely been comprehensively and quantitively studied in advanced generation synthetic lines.A better understanding of these processes will aid breeders in knowing how to best utilize synthetic allopolyploids in breeding ***,we analyzed three genetic mapping populations(735 DH lines)derived from crosses between advanced synthetic and conventional Brassica napus(rapeseed)lines,using whole-genome sequencing to determine genome *** observed high tolerance of large structural variants,particularly toward the telomeres,and preferential selection for balanced homoeologous exchanges(duplication/deletion events between the A and c genomes resulting in retention of gene/c.r.mosome dosage between homoeologous c.r.mosome pairs),including stable events involving whole c.r.mosomes("pseudoeuploidy").Given the experimental design(all three populations shared a common parent),we were able to observe that parental SV was regularly inherited,showed genetic hitchhiking effects on segregation,and was one of the major factors inducing adjacent novel and larger ***,novel SV occ.r.ed at low frequencies with no significant impacts on observed fertility and yield-related traits in the advanced generation synthetic ***,inc.r.orating genome-wide SV in linkage mapping explained significantly more genetic variance for *** results provide a framework for detecting and understanding the occ.r.ence and inheritance of genomic SV in breeding programs,and support the use of synthetic parents as an important source of novel trait variation.

关键词： Allopolyploid Large Genome structural variation Linkage mapping Synthetic polyploids Inheritance and segregation

山药多糖对地塞米松诱导Ir-3T3-L1脂肪细胞的降血糖作用及机制研究

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中国比较医学杂志 2024年第4期34卷 73-83页

作者：刘昕玥王凤忠郑涵文 Alberto c.r.os pires Dias 范蓓王琼中国农业科学院农产品加工研究所/农业农村部农产品加工质量安全风险评估实验室/农业农村部农产品质量安全收贮运管控重点实验室北京100193 西南医科大学附属中医医院中葡中医药国际合作中心四川泸州646000 葡萄牙米尼奥大学生物系中葡药食植物资源研究中心葡萄牙布拉加4710-057

目的探究山药多糖(chinese yam polysaccharide,cYPS)在地塞米松(dexamethasone,DEX)诱导的3T3-L1胰岛素抵抗(Ir-3T3-L1)细胞模型中的降血糖作用。方法将Ir-3T3-L1脂肪细胞随机分为对照组(con)、DEX组(DEX)、Met组(Met,阳性药组)、cYPS组(0.05、0.15、0.45 mg/mL),通过DEX(1μmol/L)诱导建立Ir-3T3-L1细胞模型,进行给药后细胞对葡萄糖的消耗量和细胞中血脂(总胆固醇(total cholesterol,Tc)、甘油三酯(triglyc.r.de,TG)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-c)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-c))与丙二醛(malondialdehyde,MDA)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、己糖激酶(hexokinase,HK)、丙酮酸激酶(pyruvate kinase,PK)含量的检测,并采用qrT-Pcr对AMPK-PI3K/Akt信号通路中相关基因表达进行检测。结果DEX组与con组相比,1μmol/L DEX处理48 h显著降低了Ir-3T3-L1细胞的葡萄糖消耗量(PcYPS处理Ir-3T3-L1细胞显著增加了细胞中葡萄糖的消耗(Pc含量(PcHO、TG、LDL-c含量(PcYPS可以显著回调PI3K、Akt、GLUT-4、AMPK、IrS-2、PPAra、Adiponectin的mrNA水平(PrS-1、GSK-3β、Acc、FAS的mrNA表达水平(PcYPS能够改善DEX诱导的Ir-3T3-L1细胞对葡萄糖的消耗,调节脂代谢紊乱,缓解氧化应激,其作用机制可能通过调控Ir-3T3-L1脂肪细胞的AMPK-PI3K/Akt信号通路来实现糖脂代谢紊乱和胰岛素抵抗调节来实现。

关键词：山药多糖胰岛素抵抗地塞米松 3T3-L1细胞二甲双胍 AMPK-PI3K/Akt信号通路

黄花菜醇提物对斑马鱼抑郁样行为的改善作用研究

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中国比较医学杂志 2024年第4期34卷 20-27页

作者：郑涵文王梓航刘昕玥于佳玮 Alberto c.r.os pires Dias 王凤忠王琼西南医科大学附属中医医院中葡中医药国际合作中心四川泸州646000 中国农业科学院农产品加工研究所北京100193 中国农业大学食品科学与营养工程学院北京100083 葡萄牙米尼奥大学生物系中葡药食植物资源研究中心葡萄牙布拉加4710-057

目的探究黄花菜醇提物(Hemerocallis citrina Baroni,HcE)在利血平(reserpine)诱导的斑马鱼幼鱼(Danio rerio)模型中对抑郁行为的改善作用。方法将斑马鱼幼鱼分为对照(con)组、利血平组、氟西汀组、黄花菜低剂量组(1.5 mg/L)、黄花菜... 详细信息

目的探究黄花菜醇提物(Hemerocallis citrina Baroni,HcE)在利血平(reserpine)诱导的斑马鱼幼鱼(Danio rerio)模型中对抑郁行为的改善作用。方法将斑马鱼幼鱼分为对照(con)组、利血平组、氟西汀组、黄花菜低剂量组(1.5 mg/L)、黄花菜中剂量组(3 mg/L)、黄花菜高剂量组(4.5 mg/L)。采用斑马鱼声音刺激和光刺激进行抑郁样行为学分析。利用rT-qPcr研究黄花菜醇提物对抑郁症有关的星状胶质细胞标志物及其衍生物基因(GFAP、c3、c4B、EMP-1和S100α-10)以及神经营养因子(BDNF)及其自身受体转录基因(P75、TrkB)的调节功能。结果与对照组相比,模型组在440 Hz声音刺激与8000 Lux光照刺激下运动距离缩短、运动时间减少(PrkB下调(PrkB表达显著上调(P<0.01,P<0.0001)。表明黄花菜醇提物抑制了星形胶质细胞激活诱发的炎症表现,同时促进了神经营养因子及其受体基因的生成,对抑郁起到了改善作用。结论黄花菜醇提物可以改善利血平诱导的斑马鱼幼鱼的抑郁样行为变化,斑马鱼脑中星形胶质细胞标志物的表达量减少,同时促进神经营养因子及其受体基因的生成,起到抗抑郁作用。

关键词：黄花菜抑郁症利血平斑马鱼

The ULT1 and ULT2 trxG Genes Play Overlapping roles in Arabidopsis Development and Gene regulation

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Molecular Plant 2013年第5期6卷 1564-1579页

作者： Mona M. Monfared cristel c. c.r.es Pascale rossignol Helena r. pires Jennifer c. Fletcher Plant Gene Expression center USDA-ARS/UC Berkeley 800 Buchanan Street. Albany CA 94710 USA Department of Plant and Microbial Biology University of California Berkeley 111 Koshland Hall Berkeley CA 94720 USA c.r. UMR5168 F-38054 Grenoble France Universite Grenoble Alpes UMR5168 F-38041 Grenoble France cEA iRTSV Laboratoire Physiologie Cellulaire et Vegetale F-38054 Grenoble France INrA F-38054 Grenoble France Department of Molecular Cellular and Developmental Biology Yale University New Haven CT 06520 USA Faculdade de ciencias da Universidade do Porto Porto Portugal

The epigenetic.r.gulation of gene expression is critical for ensuring the proper deployment and stability of defined genome transcription programs at specific developmental stages. The cellular memory of stable gene expression states during animal and plant development is mediated by the opposing activities of Polycomb group （PcG） factors and trithorax group （trxG） factors. Yet, despite their importance, only a few trxG factors have been characterized in plants and their rotes in regulating plant development are poorly defined. In this work, we report that the closely related Arabidopsis trxG genes ULTrAPETALA1 （ULT1） and ULT2 have overlapping functions in regulating shoot and floral stem cell accumula- tion, with ULT1 playing a major role but ULT2 also making a minor contribution. The two genes also have a novel, redun- dant activity in establishing the apical-basal polarity axis of the gynoecium, indicating that they function in differentiating tissues. Like ULT1 proteins, ULT2 proteins have a dual nuclear and cytoplasmic localization, and the two proteins physically associate in planta. Finally, we demonstrate that ULT1 and ULT2 have very similar overexpression phenotypes and regulate a common set of key development target genes, including floral MADS-box genes and class I KNOX genes. Our results reveal that c.r.matin remodeling mediated by the ULT1 and ULT2 proteins is necessary to control the development of mer- istems and reproductive organs. They also suggest that, like their animal counterparts, plant trxG proteins may function in multi-protein complexes to up-regulate the expression of key stage- and tissue-specific developmental regulatory genes.

关键词： trxG factor epigenetics stem cells Arabidopsis polarity ULTrAPETALA.

Neutrino masses,cosmological inflation and dark matter in a variant U(1)B-L model with type II seesaw mechanism

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chinese Physics c 2021年第2期45卷 621-630页

作者： J.G.rodrigues A.c.O.Santos J.G.Ferreira Jr c.A.de S.pires Departamento de Física Universidade Federal da ParaíbaCaixa Postal 500858051-970João PessoaPBBrasil Departamento de Física Universidade Federal do Rio Grande do Norte59078-970NatalRNBrasil

In this study,we implemented the type Ⅱ seesaw mechanism into the framework of the U(l)B-L gauge *** achieve this,we added a scalar triplet,A,to the canonical particle content of the U(l)B-Lgauge *** imposing that the U(l)B-L gauge symmetry be spontaneously broken at TeV scale,we show that the type Ⅱ seesaw mechanism is realized at an intermediate energy scale,more precisely,at approximately 109 *** prevent heavy right-handed neutrinos from disturbing the mechanism,we evoke a Z2 discrete ***,as a result,we have standard neutrinos with mass around eV scale and right-handed neutrinos with mass in TeV scale,with the lightest one fulfilling the condition of dark *** developed all of these in this *** addition,we show that the neutral component of Δ may perform unproblematic non-minimal inflation with loss of unitarity.

关键词： models beyond the standard model particle-theory and field-theory models of the early universe neutrino mass and mixing

The effect of fatigue and duration knowledge of exercise on kicking performance in socc.r.players

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Journal of Sport and Health Science 2019年第6期8卷 567-573页

作者： ric.r.o Manuel pires Ferraz roland van den Tillaar Ana Pereira Mario c.Marques Department of Sports Sciences University of Beira InteriorCovilha 6201-001Portugal research center in Sports Sciences Health and Human DevelopmentCIDESDVila Real 5000-801Portugal Department of Teacher Education of Nord University Levanger 7600Norway Department of Technology and Science School of Education of Polytechnic Institute of SetubalSetubal 2910-761Portugal

Purpose:The purpose of this study was to investigate the influence of fatigue upon kicking maximal ball velocity and the target-hitting acc.r.cy of socc.r.players;and also to examine the effect of the knowledge of the exercise duration upon these 2 ***:Twenty-four semi-professional socc.r.players participated in this study and performed maximal instep kicks before and after the implementation of an exercise protocol,either with or without knowledge of the duration of this ***:A mixed model of analysis of variance showed that kicking maximal ball velocity was significantly affected(F(5,85)=11.6,pr just 1 c.r.uit of the fatigue protocol and then remained *** did not change during the protocol(F(5,75)=0.23,p=0.76,η^2=0.03) and knowing the duration of exercitation did not affect acc.r.cy and velocity development(F(1,23) ≤1.04,p≥0.32,η~2≤0.06).conclusion:These findings demonstrated the potential negative effects of fatigue on kicking ball velocity in socc.r.but not in the kicking acc.r.cy and that the effect of fatigue may not be progressive over *** or not knowing the duration of exercitation did not affect the results.

关键词： Fatigue Kicking ball velocity Pacing Perception of effort Self-regulation of effort

新型分层ZSM-5沸石的合成及其在去除三氯乙烯中的应用（英文）

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催化学报 2014年第9期35卷 1492-1496页

作者： Joo pires Ana c. Fernandes Divakar Duraiswami centro de Química e Bioquímica Faculdade de CiênciasUniversidade de Lisboa1749-016 LisboaPortugal Department of chemistry and Applied chemistry PSG College of TechnologyPeelameduCoimbatore 641004India

A self-supporting ZSM-5 monolith with a hierarchical porosity was prepared using polyurethane foam (PUF) as a structural template and a hydrothermal synthesis procedure. The synthesized monolith was characterized and investigated towards the adsorption and catalytic oxidation of trichloroethylene (TcE). Adsorption of TcE was studied gravimetrically and oxidation of TcE was studied using a vapor-phase down-flow reactor. Monolithic ZSM-5 displayed good sorption properties and completely oxidized TcE. conversion levels of 50% and 90% were achieved at reduced temperatures (by ∼50 °c) when compared with the conversion temperatures obtained from the powder counterparts. Besides the activity of the monolith towards TcE adsorption and oxidation, it was stable and enhanced diffusion, thereby reducing pressure drops to a great extent owing to its hierarchical porous nature.

关键词： ZSM-5 三氯乙烯合成方法整料应用催化氧化聚氨酯泡沫重量分析法

Species richness and density evaluation for plants with aggregated distributions:fixed *** area methods

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Journal of Plant Ecology 2017年第5期10卷 765-770页

作者： rita de cássia Quitete Portela Alexandra dos Santos pires Maria Isabel Guedes Braz Eduardo Arcoverde de Mattos Departamento de Ecologia Instituto de BiologiaUniversidade Federal do Rio de JaneiroCaixa Postal 68020Ilha do FundãoRio de JaneiroRJ 21941-590Brasil Departamento de ciências Ambientais Instituto de FlorestasUniversidade Federal Rural do Rio de JaneiroRod.BR 465Km 07Cidade UniversitáriaSeropédicaRio de JaneiroRJ 23890-000Brasil

Aims Knowledge of species richness is of prime importance to both basic and applied aspects of ecological ***,quantifying plant species richness in the tropics is potentially time-consuming because of high species *** species richness estimates are also frequently biased,because many rare species are not *** address these problems,the use of a variable area method has been proposed as an alternative to fixed area methods,but its appli-cability to plants with aggregated distributions has been questioned based on simulation *** use empirical data to compare the efficiency and acc.r.cy of a variable area method and a fixed area method for estimating species richness,density and basal area for plants with aggregated distributions,using palms as a model *** Adult palms were sampled in twenty 10×30 m transects in an Atlantic Forest in rio de Janeiro state,*** individuals were considered in the fixed area method,while in the variable area method only the first six adults in each transect were sampled;in this case,transect length was defined acc.r.ing to the distance of the sixth adult from the beginning of the *** fewer than six individuals were observed in a given transect,transect length was extended up to 50 m to search for additional *** efficiency of both methods was compared based on species rarefaction c.r.es,using the chao 1 statistic(for abundance data).For each species we calculated mean density per transect and basal area,acc.r.ing to each sampling *** effort in terms of the number of individual plants and the area necessary to characterize maximum species richness in each sampling method,as well as mean time taken to sample a single transect,were compared as measures of *** findings An acc.r.te estimate of species richness was achieved using both methods,but in the variable area method,a quarter of the number of individuals and half the area was sufficient

关键词： basal area biodiversity inventory palms taxon sampling

Effect of combined Use of Brackish Water and Nitrogen Fertilizer on Biomass and Sugar Yield of Sweet Sorghum

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Pedosphere 2012年第6期22卷 785-794页

作者： T.B.rAMOS N.L.cASTANHEIrA M.c.GONALVES M.L.FErNANDES M.I.JANUrIO M.E.LOUrENO F.P.pires J.c.MArTINS cEEr-Biosystems Engineering Institute of Agronomy Technical University of Lisbon Tapada da Ajuda 1349-017 Lisbon (Portugal) Estao Agronómica Nacional L-INIA Instituto Nacional de Recursos Biológicos Quinta do Marquês Av. República 2784-505 Oeiras (Portugal) Departamento de Fitotecnia Instituto de Ci encias Agrrias e Ambientais Mediterrnicas Universidade de vora Apartado 94 7002-554 vora (Portugal)

Soil salinization and non-point source pollution are among the most important and widespread environmental problems in European Mediterranean regions. Sweet sorghum （Sorghum bicolor （L.） Moench var. saccharatum） is a moderate to high salinity tolerant crop with low water and nutrient needs, seen as an alternative to grow in the water sc.r.e regions. A three-year multifactorial study was conducted in southern Portugal to evaluate the combined effects of saline water and nitrogen application on the dry biomass （total, stems, and leaves）, sugar content （total reducing sugars and sucrose eontents） and sugar yield （here defined as the product of total reducing sugars and stems dry biomass） functions of sweet sorghum. Sorghum dry biomass and sugar yield showed diminishing returns for each incremental change of nitrogen. The use of saline irrigation waters also led to yield reduction. Exception was sucrose content which increased with increasing levels of sodium in the soil. Nitrogen need decreased as the amount of sodium applied increased. Stem dry biomass, sucrose content, and sugar yield progressively increased with progress in the experiment. The effect could be attributed to the increase of the amount of irrigation applied throughout the years, thus increasing the leaching fraction which promoted salt leaching from the root zone, reduced the salinity stress, increased plant transpiration, nitrogen uptake and biomass yield.

关键词： Mediterranean conditions non-point source pollution salinity sweet sorghum yield functions