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The SWi2/SNF2 Chromatin-Remodeling ATPase BRAHMA Regulates Chlorophyll Biosynthesis in Arabidopsis

Molecular Plant 2017年第1期10卷 155-167页

作者： Dong zhang Yuhong Li Xinyu zhang ping zha Rongcheng Lin Key Laboratory of Photobiology CAS Center for Excellence in Molecular Plant Sciences Institute of Botany The Chinese Academy of Sciences Beijing 100093 China College of Life Sciences University of the Chinese Academy of Sciences Beijing 100049 China These authors contributed equally to this article

Chlorophyll biosynthesis is critical for chloroplast development and photosynthesis in plants. Although reactions in the chlorophyll biosynthetic pathway have been largely known, little is known about the regu-latory mechanisms of this pathway. In this study, we found that the dark-grown knockout and knockdown mutants as well as RNA-interference transgenic seedlings of BRAHMA （BRM）, which encodes an SWI2/ SNF2 chromatin-remodeling ATPase, had iligher greening rates, accumulated less protochlorophyllide, and produced less reactive oxygen species than Arabidopsis wild-type plants did upon light exposure. The expression of NADPH：protochlorophyilide oxidoreductase A （PORA）, PORB, and PORC, which catalyze a key step in chlorophyll biosynthesis, was increased in the brm mutants. We found that BRM physically interacted with the bHLH transcription factor PHYTOCHRONIE-iNTERACTING FACTOR 1 （PIF1） through its N-terminal domains. Furthermore, we demonstrated that BRM was directly recruited to the cis-regula-tory regions of PORC, but not of PORA and PORB, at least partially in a PIF1-dependent manner and the level of histone H3 lysine 4 tri-methylation （H3K4me3） at PORC loci was increased in the brm mutant. Taken together, our data indicate that the chromatino-remodeling enzyme BRM modulates PORC expression through interacting with PIF1, providing a novel regulatory mechanism by which plants fine-tune chloro-phyll biosynthesis during the transition from heterotrophic to autotrophic growth.

关键词： chromatin remodeling chlorophyll biosynthesis light signaling PIF1 PORC

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Effect of different ventilation methods combined with pulmonary surfactant on neonatal acute respiratory distress syndrome

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World Journal of Clinical Cases 2023年第25期11卷 5878-5886页

作者： Qing Qing ping zha Li-Ying Dai Yang Wang Department of Pediatrics The First Affiliated Hospital of Anhui Medical UniversityHefei 230001Anhui ProvinceChina Department of Neonatology Anhui Provincial Children's HospitalHefei 230001Anhui ProvinceChina

BACKGROUND Acute respiratory distress syndrome precipitates is widespread pulmonary injury in impacted individuals,the neonatal respiratory distress syndrome(NRDS),primarily observed in preterm infants,represents a prevalent critical condition in neonatal clinical *** To investigate the clinical efficacy of various ventilation strategies combined with pulmonary surfactant(PS)therapy in the treatment of *** A total of 20 neonates diagnosed with respiratory distress syndrome,admitted between May 2021 and June 2022,were randomly assigned to either a research group or a control *** in the research group received treatment involving high-frequency oscillatory ventilation(HFOV)in conjunction with *** contrast,neonates in the control group were administered either controlled mechanical ventilation or synchronous intermittent mandatory ventilation,combined with *** blood samples from the neonates in both groups were collected before treatment,as well as 6 h,12 h,24 h,and 48 h *** samples underwent blood gas analysis,with measurements taken for pH value,partial pressures of oxygen(O_(2))and carbon ***,data was collected on the duration of ventilator use,length of hospitalization time,O_(2) treatment time,treatment outcomes,and complications of the *** From 6-48 h post-treatment,both groups demonstrated significant improvements in arterial blood pH and oxygen partial pressure,along with a significant decrease in carbon dioxide partial pressure compared to pre-treatment values(P0.05).However,the research group had significantly lower X-ray scores,shorter hospitalization time,and less time on O_(2) therapy compared to the control group(P0.05),but the research group had a significantly lower incidence of complications(P<

关键词： Neonatal respiratory distress syndrome Pulmonary surfactant Mechanical ventilation Respiratory distress syndrome Acute respiratory distress syndrome

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SEUSS and PIF4 Coordinately Regulate Light and Temperature Signaling Pathways to Control Plant Growth

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Molecular Plant 2018年第7期11卷 928-942页

作者： Junling Huai Xinyu zhang dialong Li Tingting Ma ping zha Yanjun Jing Rongcheng Lin Key Laboratory of Photobiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China University of Chinese Academy of Sciences Beijing 100049 China GAS Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences Beijing 100093 China These authors contribute equally to this article.

Plants continuously monitor environmental conditions （such as light and temperature） and adjust their growth and development accordingly. The transcription factor PHYTOCHROME-INTERACTING FACTOR4 （PIF4） regulates both light and temperature signaling pathways. Here, we identified ENHANCED PHOTOMORPHOGENIC2 （EPP2） as a new repressor of photomorphogenesis in red, far-red, and blue light. Map-based cloning revealed that EPP2 encodes the SEUSS （SEU） transcription regulator. The C terminus of SEU has transcriptional activation activity, and SEU physically interacts with PIF4. Moreover, SEU promotes the expression of many genes, including auxin biosynthetic and responsive genes, and regulates IAA levels in plants. SEU associates with the regulatory regions of INDOLE-3-ACETIC ACID INDUCIBLE6 （IAA6） and IAA 19 in a PIF4-independent manner, whereas the binding of PIF4to these genes requires SEU. Furthermore, muta- tions in SEU affect H3K4me3 methylation at IAA6 and IAA 19, and SEU positively regulates warm temperature- mediated hypocotyl growth together with PIF4. Collectively, our results reveal that SEU acts as a central regulator integrating light and temperature signals to control plant growth by coordinating with PIF4.

关键词： light temperature SEU PIF4 transcription regulation

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The Evening Complex and the Chromatin-Remodeling Factor PICKLE Coordinately Control Seed Dormancy by Directly Repressing DOG1 in Arabidopsis

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Plant Communications 2020年第2期1卷 54-65页

作者： ping zha Shuangrong Liu Yang Li Tingting Ma Liwen Yang Yanjun Jing Rongcheng Lin Key Laboratory of Photobiology Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China CAS Center for Excellence in Molecular Plant Sciences Institute of BotanyChinese Academy of SciencesBeijing 100093China

Primary seed dormancy is acquired during seed development and maturation,which is important for plant fitness and *** OF GERMINATION1(DOG1)plays a critical role in inducing seed ***1 expression increases rapidly during seed development,but the precise mechanism underlying this process remains *** this study,we showed that mutants with a loss or reduced function of the chromatin-remodeling factor PICKLE(PKL)exhibit increased seed *** associates with DOG1 chromatin and inhibits its *** found that PKL physically interacts with LUX ARRHYTHMO(LUX),a member of the evening complex(EC)of the circadian ***,LUX directly binds to a specific coding sequence of DOG1,and DOG1 acts genetically downstream of PKL and *** in either LUX or EARLY FLOWERING3(ELF3)encoding another member of the EC led to increased DOG1 expression and enhanced seed ***,these phenotypes were abolished when the parent plants were grown under continuous *** addition,we observed that loss of function of either PKL or LUX decreased H3K27me3 levels at the DOG1 *** together,our study reveals a regulatory mechanism in which EC proteins coordinate with PKL to transmit circadian signals for directly regulating DOG1 expression and seed dormancy during seed development.

关键词： seed dormancy chromatin remodeling clock DOG1

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Acute appendicitis in the short term following radical total gastrectomy misdiagnosed as duodenal stump leakage:A case report

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World Journal of Gastrointestinal Surgery 2022年第12期14卷 1432-1437页

作者： Jun Ma Zhen-ping zha Chao-ping Zhou Xiang Miao Shu-Qiang Duan Ya-Ming zhang Department of General Surgery Anqing Municipal HospitalAnqing 246000Anhui ProvinceChina Department of Vascular Surgery Anqing Municipal HospitalAnqing 246000Anhui ProvinceChina Department of Pathology Anqing Municipal HospitalAnqing 246000Anhui ProvinceChina

BACKGROUND Common diseases after radical gastrectomy include cholecystitis and pancreatitis,but the sudden onset of acute appendicitis in a short period following radical gastrectomy is very rare,and its clinical symptoms are easily misdiagnosed as duodenal stump *** SUMMARY This is a case report of a 77-year-old woman with lower right abdominal pain 14 d after radical resection of gastric *** pain was not relieved by conservative treatment,and her inflammatory markers were *** tomography showed effusion in the perihepatic and hepatorenal spaces,right paracolic sulcus and pelvis,as well as exudative changes in the right iliac *** puncture revealed a slightly turbid yellow-green *** exploration showed a swollen appendix with surrounding pus moss and no abnormalities of the digestive anastomosis or stump;thus,laparoscopic appendectomy was *** patient recovered well after the *** pathology showed acute purulent *** patient continued adjuvant chemotherapy after surgery,completing three cycles of oxaliplatin plus S-1(SOX regimen).CONCLUSION Acute appendicitis in the short term after radical gastrectomy needs to be differentiated from duodenal stump leakage,and early diagnosis and surgery are the most important means of treatment.

关键词： Gastric cancer Acute appendicitis Surgery Complications Case report

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Multiple Episodes of Zircon Growth during Anatectic Metamorphism of Metasedimentary Rocks in Collisional Orogens:Constraints from Felsic Granulites in the Bohemian Massif

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Journal of Earth Science 2023年第3期34卷 609-639页

作者： Guang Yang Ren-Xu Chen Yong-Fei Zheng Qiong-Xia Xia Yong-Jie Yu Kun Li zhaochu Hu Bing Gong Xiang-ping zha CAS Key Laboratory of Crust-Mantle Materials and Environments School of Earth and Space SciencesUniversity of Science and Technology of ChinaHefei 230026China Center of Excellence for Comparative Planetology Chinese Academy of Sciences(CAS)Hefei 230026China Frontiers Science Center for Planetary Exploration and Emerging Technologies University of Science and Technology of ChinaHefei 230026China State Key Laboratory of Geological Processes and Mineral Resources Faculty of Materials Science and ChemistryChina University of GeosciencesWuhan 430078China

Zircon is a key accessary mineral for metamorphic geochronology and geochemical tracing,but it has been a challenge to interpret its complex chemical zoning and age record acquired during multiple episodes of anatectic metamorphism in collisional *** is illustrated by a combined study of petrography,phase equilibrium modeling and metamorphic P-T-t determination for granulites from the Bohemian Massif in the Variscan *** rocks record multiple episodes of zircon growth during anatectic *** started from the compressional heating for prograde metamorphism to high-pressure(HP)to ultrahigh-pressure(UHP)eclogite facies with low degrees of partial ***,they underwent a decompressional stage from UHP eclogite facies to HP granulite facies for dehydration *** were followed by a further decompressional stage either to kyanite granulite facies or to sillimanite granulite facies at ultrahigh-temperature(UHT)*** of zircon growth are linked to specific metamorphic conditions for peritectic reactions on the basis of zoning patterns,trace element signatures,index mineral inclusions in dated domains and textural relationships to coexisting *** results indicate that relict zircon domains are preserved even at UHT granulite facies conditions.A few zircon domains in the kyanite granulite grew during the prograde to peak UHP metamorphism,possibly corresponding to consumption of biotite and plagioclase but growth of *** the decompressional exhumation to the HP granulite-facies,relict or prograde zircon domains were mostly dissolved into anatectic melts produced by muscovite *** zircon grains grew during this transition to the HP granulite-facies in the kyanite granulite and are chemically related to continuous growth of garnet,whereas abundant zircon grains grew subsequently at the UHT granulite facies in the sillimanite granulite and are chemically related to garnet breakdown reactions.A

关键词： ultrahigh-pressure metamorphism ultrahigh-temperature metamorphism felsic granulite zircon continental collision crustal anataxis

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唐人婚姻习俗与李白成名前的家庭生活——李白《寄远十二首》考释

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复旦学报（社会科学版） 2010年第5期52卷 41-52页

作者：查屏球复旦大学中文系上海200433

李白以布衣客籍之士的身份与已故宰相许圉师孙女成婚,并在婚后长期居于安陆妻子家中。李白与许府的结缘在于许家中落与唐时相对宽松的士籍管理制度。初盛唐时贵族高门有招才俊之士为婿的风尚。"夫随妻居",或者"夫居妻家"、"寄室妻家",... 详细信息

李白以布衣客籍之士的身份与已故宰相许圉师孙女成婚,并在婚后长期居于安陆妻子家中。李白与许府的结缘在于许家中落与唐时相对宽松的士籍管理制度。初盛唐时贵族高门有招才俊之士为婿的风尚。"夫随妻居",或者"夫居妻家"、"寄室妻家",是已婚士人在出名前的一种生活方式。他们同时承担了为妻家争得荣誉、提升妻家的社会地位和门户声望的责任。这种因婚姻生活而带来的功名压力,也反映在李白的早期作品中。从这一角度,可判定其组诗《寄远十二首》应作于早年,反映了他在外游历时对妻子的思念,以及妻子去世后的悼念之意。部分"闺情"诗,则可能是其早年与许氏的游戏之作。这一生活方式使之摆脱了家庭之累,诗人浪漫的才情也得以尽情发挥。

关键词：李白婚姻夫随妻居悼亡诗

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西周金文与“修辞立其诚”的原始意义

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学术探索 2010年第3期 125-133页

作者：查屏球复旦大学中国古代文学研究中心上海200433

西周铭文是现存较早的单独成篇的文本,《易传.文言》中"修辞立其诚"之观念虽是后起之说,但其所强调的"诚"应与早期铭文之类写作活动相关,可能就是对这类文本写作经验的总结。西周铭文是祭祀者与神对话的记录,以诚心获取神之佑助是铭文... 详细信息

西周铭文是现存较早的单独成篇的文本,《易传.文言》中"修辞立其诚"之观念虽是后起之说,但其所强调的"诚"应与早期铭文之类写作活动相关,可能就是对这类文本写作经验的总结。西周铭文是祭祀者与神对话的记录,以诚心获取神之佑助是铭文写作的动机,向神展示诚心是其核心的内容,诚也是铭文行文的基本要求与行文风格,故借助西周铭文,可以为这一文论观念作更具体的文化溯源。

关键词：祭祀文化铭文特色修辞立诚

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航速优化下小型船舶调度目标选取优化方法分析

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舰船科学技术 2021年第14期43卷 34-36页

作者：查安平广东交通职业技术学院海事学院

航运公司在进行货物运输时,为了提高航运经济性,降低营运成本,会对海域内的船舶进行灵活调度,对每艘船的航线、航行速度、途径码头、港口、货物运载量等内容进行详细的控制,船舶调度控制也是一项航运领域的研究热点。本文提出一种基于... 详细信息

航运公司在进行货物运输时,为了提高航运经济性,降低营运成本,会对海域内的船舶进行灵活调度,对每艘船的航线、航行速度、途径码头、港口、货物运载量等内容进行详细的控制,船舶调度控制也是一项航运领域的研究热点。本文提出一种基于遗传算法的小型船舶调度优化技术,通过建立船舶的调度数学模型和目标函数,实现了航速优化条件下的船舶调度目标快速化选择。

关键词：航速优化船舶调度遗传算法目标选择

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中国式学校体育现代化中的“五育并举”

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武汉体育学院学报 2024年第3期58卷 20-25页

作者：查萍梁凤波北京师范大学体育与运动学院北京100875 北京交通大学体育教研部北京100044

历经四个发展阶段,党的教育方针从德、智、体“三育全面发展”拓展成德、智、体、美、劳“五育并举”,这是中国教育在新时代面对人才培养的现实主题,是应对新征程中国教育的发展要求而进行的目标拓展和改革举措。研究发现,从中国式学校... 详细信息

历经四个发展阶段,党的教育方针从德、智、体“三育全面发展”拓展成德、智、体、美、劳“五育并举”,这是中国教育在新时代面对人才培养的现实主题,是应对新征程中国教育的发展要求而进行的目标拓展和改革举措。研究发现,从中国式学校体育现代化的视角来看,“五育”中的体育是一个极具特殊性的教育和学科,它在功能上与德育、智育、美育及劳动教育有着深刻内在的结构性联系,因此它具有与其他“四育”可以深入融合共育的广泛可能性。鉴于推进“五育并举”过程中会遇到的结构性障碍与困难,分别提出了体育与其他“四育”并举并进的途径与方法,推进体育成为“五育并举”的主渠道、主战场和主力军。

关键词：五育并举发展阶段全面发展教育方针德智体美劳中国式学校体育现代化

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