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The protective effect of ginsenoside Rb1 pre-treatment on myocardial ischemia/reperfusion injury in Sprague-Dawley rats

中国神经科学学会第十届全国学术会议

作者： Xiao-Lan WANG Zhi-Hua YANG Guang-pin CHU Xian-fang MENG Department of Neurobiology School of Basic Medical SciencesTongji Medical CollegeHuazhong University of Science and Technology

Objective Diabetic encephalopathy may lead to cognitive deficits in diabetic patients and diminish quahty of *** has been shown that protracted hyperglycemia is directly associated with neuronal apoptosis,which is involved in diabetic ***1/APC is essential for the survival of postmitotic *** our previously study,we found that MAD2B,one of APC inhibitors,was expressed in neurons in central nervous ***,whether MAD2B is involved in hyperglycemia-induced apoptosis and thus takes part in diabetic encephalopathy is still *** To address this issue,we first explored the expression of MAD2B and cyclin Bl detected by immunofluorescence and Western blot in vivo and in vitro under *** further explore the role of MAD2B in hyperglycemia-induced neuronal injury,we depleted MAD2B expression by a specifically targeted shRNA against MAD2B in cultured *** we detected neuron apoptosis by TUNEL analysis and S phase with EdU *** It was found that hyperglycemia remarkably increased the expression of MAD2B and accumulation of cyclin Bl in cortices of diabetes mellitus rat model and in cultured primary *** results demonstrate that MAD2B expression is the main culprit for accumulation of cyclin B1 and apoptosis in neurons under high *** observed that MAD2B deficiency alleviated cyclin Bl expression and apoptotic neuronal ***,the increased expression of MAD2B promoted neurons to enter an aberrant S phase that led differentiated neurons into apoptotic cell *** These results suggest that hyperglycemia induced neuronal apoptosis through inducing expression of MAD2B,which represents a novel mechanism of diabetic encephalopathy.

关键词： Key ords:MAD2B hyperglycaemia cyclin B1 neuronal injury

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South China Journal of Cardiology 2020年第1期21卷 53-59,66页

作者： fang pin-hao LI Chang-yan YANG Jia-ni MENG Wen-ting OU Ding-qin Departments of Bioengineering Kunming Medical University Graduate school of Kunming Medical University Kunming 650500YunnanChina

Background Myocardial ischemia/reperfusion injury(MI/RI)during myocardial infarction worsens outcomes. It has been proved that ginsenoside Rb1 has a great impact on ischemia/reperfusion(I/R)injury. The aim of this study is to explore the protective effect of the pretreatment with ginsenoside Rb1 on MI/RI and investigate the underlying mechanisms about the preventive action. Methods A total of 27 healthy adult Sprague-Dawley(SD)rats were randomly divided into 3 groups(n=9 per group):A sham-operated group(Sham n=9);an ischemia 40 min/reperfusion 2 h(I/R n=9)of the cardiac muscle group;Rb1-treated group was divided into 3 subgroups(Rb1 10 mg/kg,20 mg/kg,40 mg/kg,n=3). Ginsenosides Rb1 at different concentrations were injected intraperitoneally for 3 days continuously before ligation. A model of MI/RI was constructed by ligation of the left coronary artery anterior descending branch in rats. Myocardial infarction area after I/R was measured bytriphenyltetrazolium chloride(TTC)staining of myocardial tissue. Hematoxylin and eosin(HE)staining and electrocardiogram indication were used to observe myocardial cell injury and ischemia,respectively. The expression of caspase-8 protein was observed by immunohistochemistry staining. Results The pretreatment of 40 mg/kg dose of ginsenoside Rb1 could decrease the expression of caspase-8 protein caused by I/R and the apoptosis of myocardial cells,improve myocardial ischemia,reduce the area of myocardial infarction and ameliorate MI/*** These results demonstrate that ginsenoside Rb1 has a significant protective effect on MI/RI through attenuating the apoptosis of myocardial cells and improving myocardial ischemia at appropriate dose,which provides new insights into the potential therapy of MI/RI.

关键词： myocardial ischemia/reperfusion injury ginsenoside Rb1 caspase-8 protein TTC staining HE staining

现代化农业背景下都市型农业高质量发展标准体系建设与应用——以北京市为例

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中国标准化 2024年第19期 201-206页

作者：于寒冰沙品洁郝建强肖帅李小凤王芳于晓雷张国光张金轶北京市农产品质量安全中心

北京市以满足都市型现代农业发展为目标,以新质生产力夯实农业基础,以农业生产全要素生产率大幅提升为核心,建成突出质量安全和绿色发展的现代农业标准体系。在作物种子、畜禽种源、农业现代化设施设备、数字农业等驱动乡村振兴的关键... 详细信息

北京市以满足都市型现代农业发展为目标,以新质生产力夯实农业基础,以农业生产全要素生产率大幅提升为核心,建成突出质量安全和绿色发展的现代农业标准体系。在作物种子、畜禽种源、农业现代化设施设备、数字农业等驱动乡村振兴的关键着力点均建立高质量标准体系,充分发挥新质生产力在促进农业产业融合中的作用。本文在现代农业高质量发展背景下,对北京市高质量标准体系建设进行研究应用,总结了北京市农业标准化的实践进展,分析了存在的问题,并对推进北京市都市型现代农业的标准化模式进行了思考。

关键词：新质生产力农业高质量发展农业标准化都市农业标准体系

OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models

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World Journal of Gastroenterology 2008年第30期14卷 4800-4804页

作者： Jun Shi pin-Kang Wei Shen Zhang Zhi-Feng Qin Jun Li Da-Zhi Sun Yan Xiao Zhi-Hong Yu Hui-Ming Lin Guo-Jing Zheng Xiao-Mei Su Ya-Lin Chen Yan-fang Liu Ling Xu Department of Traditional Chinese Medicine Shanghai Changzheng Hospital Shanghai 200003 China

AIM： To establish nude mouse human gastric cancer orthotopic transplantation models using OB glue paste technique. METHODS： Using OB glue paste technique, orthtopic transplantation models were established by implanting SGC-7901 and NKN-45 human gastric cancer cell strains into the gastric wall of nude mice. Biological features, growth of the implanted tumors, the success rate of transplantation and the rate of auto-metastasis of the two models were observed. RESULTS： The success rates of orthotopic transplanration of the two models were 94.20% and 96%. The rates of hepatic metastasis, pulmonary metastasis, peritoneal metastasis, lymphocytic metastasis and splenic metastasis were 42.13% and 94.20%, 48.43% and 57.97%, 30.83% and 36.96%, 67.30% and 84.06%, and 59.75% and 10.53%, respectively. The occurrence of ascites was 47.80% and 36.96%. CONCLUSION： OB glue paste technique is easy to follow. The biological behaviors of the nude mouse human gastric cancer orthotopic transplantation models established with this technique are similar to the natural processes of growth and metastasis of human gastric cancer, and, therefore, can be used as an ideal model for experimental research of proliferative metastasis of tumors.

关键词： Gastric tumor Tumor transplantation Disease models Animal Nude mice

聚氨酯/LiCF_3SO_3电解质体系的导电机理

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上海交通大学学报 2001年第4期35卷 570-573页

作者：方滨王新灵唐小真竺品芳上海交通大学化学化工学院上海200240 上海交通大学分析测试中心上海200030

利用 FT- Raman、FT- IR和复数阻抗谱 ,对聚氧化乙烯聚氨酯 /Li CF3SO3固体电解质样品中离子在聚合物电解质中的导电机理进行了研究 .发现导电离子以自由离子、离子对和聚集体的形式存在于体系中 ,离子状态随盐浓度不同发生变化 ,自由... 详细信息

利用 FT- Raman、FT- IR和复数阻抗谱 ,对聚氧化乙烯聚氨酯 /Li CF3SO3固体电解质样品中离子在聚合物电解质中的导电机理进行了研究 .发现导电离子以自由离子、离子对和聚集体的形式存在于体系中 ,离子状态随盐浓度不同发生变化 ,自由离子比率随温度的上升而下降 ;对离子的存在状态与电导率之间的关系进行了研究 ,发现体系的电导率随自由离子的比率上升而变大 ,初步探讨了离子在体系中的导电机理 ;对 Nernst- Einstein公式在聚合物电解质体系的应用做了校正 ,建立了合理的自由离子浓度和电导率的关系 .

关键词：聚氨酯离子电导率聚合物固体电解质导电机理锂电池自由离子离子对

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几何联合分段亮度的线阵图像配准

中国图象图形学报 2024年第1期29卷 80-94页

作者：房磊史泽林刘云鹏李晨曦赵恩波张英迪东北大学机器人科学与工程学院沈阳110169 中国科学院光电信息处理重点研究室沈阳110016 中国科学院沈阳自动化研究所沈阳110016 中国科学院机器人与智能制造创新研究院沈阳110169

目的以非平行于目标的姿态成像时,线阵相机采集的图像的几何变换规律与面阵相机不同,这导致面阵图像的几何变换模型及其直接配准方法无法实现线阵图像的配准;同时,亮度恒常假设无法解决大视场镜头引起的图像亮度衰减问题。因此,提出了... 详细信息

目的以非平行于目标的姿态成像时,线阵相机采集的图像的几何变换规律与面阵相机不同,这导致面阵图像的几何变换模型及其直接配准方法无法实现线阵图像的配准;同时,亮度恒常假设无法解决大视场镜头引起的图像亮度衰减问题。因此,提出了一种几何联合分段亮度的线阵图像直接配准方法。方法根据线阵图像的几何变换模型和分段增益—偏置亮度模型,将线阵图像的配准问题表示为一个非线性最小二乘问题。采用高斯—牛顿法对配准问题中的几何变换参数和亮度变换参数联合进行优化;此外,针对以单位变换为初始值时配准图像存在较大几何误差致使优化不收敛,设计了一种初始值快速搜索策略。结果实验数据包含本文采集的线阵图像数据集和真实列车线阵图像。配准结果表明,采用本文方法配准后的标注点坐标均方根误差均小于1个像素,优于采用面阵图像几何变换模型的直接配准方法。算法对亮度变化具有更强的鲁棒性,提高了线阵图像配准的成功率。结论本文提出的几何联合分段亮度线阵图像配准方法可以精确、鲁棒地对齐非平行姿态线阵相机所采集的图像。

关键词：线阵相机线阵图像直接配准方法几何变换亮度变换

In vitro evaluation of artificial joints: a comprehensive review

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Advances in Manufacturing 2019年第1期7卷 1-14页

作者： Gang Shen Ju-Fan Zhang Feng-Zhou fang Centre of Micro/Nano Manufacturing Technology (MNMTDublin) University College Dublin Dublin Ireland State Key Laboratory of Precision Measuring Technology and Instruments Centre of Micro/Nano Manufacturing Technology (MNMT) Tianjin University Tianjin 300072 People's Republic of China

Development of artificial joints is a great progress for joint replacement operations of human being, but the short longevity of prostheses has concerned both industries and researchers since the advent of modem implants. Thus, continuous improvements have been made alongside the clinical applications. The methodologies to inspect the potential properties of new designs have also seen many advances. The test machines have evolved from easy pin-on-disk configuration to the modem joint simulator. Besides, various wear traces provided by testing machines are investigated greatly, and multi-directional files are recommended as motion profiles for in vitro testing. The typical testing parameters, like sliding speed or loading profile, are discussed in this article, and their working mechanisms are described in detail. Meanwhile, the calf or bovine serum has been regarded as the gold standard for testing lubricant, and the future trend of fluid will be focused on investigating the effects of lubricant composites on the tribological properties. Hard particles and proteins are discussed as well, and their effects on the wear mechanisms are the focus. Finally, various approaches to measure the wear rate, wear factor and the thickness of lubricant film are presented, and suggestions are given for the development in future.

关键词： Artificial joint Methodology Testing parameter Wear rate

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基于多分类支持向量机的电压暂降源识别

电力系统保护与控制 2010年第22期38卷 151-155页

作者：吕干云方奇品蔡秀珊浙江师范大学数理与信息工程学院浙江金华321004

S变换具有很好的时频分析能力,能精确提取突变信号的关键特征信息。在分析S变换原理基础上,提出一种基于多分类支持向量机的电压暂降源识别方法。用S变换对电压暂降信号进行时频分析,提取各类暂降特征;用多分类支持向量机对特征进行训... 详细信息

S变换具有很好的时频分析能力,能精确提取突变信号的关键特征信息。在分析S变换原理基础上,提出一种基于多分类支持向量机的电压暂降源识别方法。用S变换对电压暂降信号进行时频分析,提取各类暂降特征;用多分类支持向量机对特征进行训练与识别。通过仿真算例验证,该方法能有效识别电压暂降源,可应用于电能质量监测系统。

关键词：电能质量 S变换多分类支持向量机电压暂降源识别

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高功率微波频率对pin限幅器的影响分析

电子科技大学学报 2024年第3期53卷 321-326页

作者：张兵方子璇彭帅陆凤波高文泽赵银峰王树龙中国船舶集团有限公司系统工程研究院北京100094 海军研究院北京100161 西安电子科技大学微电子学院西安710071

通过TCAD器件建模,建立了pin二极管的电热耦合模型,搭建pin限幅器HPM耦合仿真电路以进行高功率微波效应仿真。仿真得到了不同频率下pin限幅器瞬态电压响应以及pin二极管内部电场、载流子、结温分布。仿真结果表明,在HPM耦合电压作用下,... 详细信息

通过TCAD器件建模,建立了pin二极管的电热耦合模型,搭建pin限幅器HPM耦合仿真电路以进行高功率微波效应仿真。仿真得到了不同频率下pin限幅器瞬态电压响应以及pin二极管内部电场、载流子、结温分布。仿真结果表明,在HPM耦合电压作用下,pin限幅器平顶泄漏负向电压随频率增大而降低,pin限幅器平顶泄漏正向电压随频率增大而降低。pin二极管内部pin限幅器的限幅能力随微波频率增加先增强后减弱。HPM频率较低时pin二极管内部晶格温度积累集中于I层界面处,随着频率升高,晶格温度积累集中于I层中间位置,因此频率变化会造成pin限幅器的HPM毁伤敏感位置变化。

关键词：高功率微波 pin限幅器电热耦合模型平顶泄漏电压