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Molecular cloning and characterization of the putative Halloween gene Phantom from the small brown planthopper Laodelphax striatellus

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Insect Science 2015年第6期22卷 707-718页

作者： Shuang Jia pin-Jun wan Guo-Qing Li Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests College of Plant Protection Nanjing AgriculturalUniversity Nanjing 210095 China

Ecdysteroid hormone 20-hydroxyecdysone plays fundamental roles in in- sect postembryonic development and reproduction. Several cytochrome P450 mono- oxygenases （CYPs）, encoded by the Halloween genes, have been documented to be involved in ecdysteroidogenesis in representative insects in Diptera, Lepidoptera and Orthoptera. Here the putative Halloween gene Phantom （Phm, cyp306al） from a hemipteran insect species, the small brown planthopper Laodelphax striatellus, was cloned. LsPHM shows five insect conserved P450 motifs, that is, Helix-C, Helix-I, Helix-K, PERF and heme- binding motifs. Temporal and spatial expression patterns of LsPhm were evaluated by quantitative polymerase chain reaction. Through the fourth-instar and the early fifth-instar stages, LsPhm showed two expression peaks in day 2 and days 4-5 fourth-instar nymphs, and three troughs in day 1 and 3 fourth instars and day 1 fifth instars. On day 5 of the fourth-instar nymphs, LsPhm clearly had a high transcript level in the thorax where the prothoracic glands were located. Dietary introduction of double-stranded RNA （dsRNA） of LsPhm at the nymph stage successfully knocked down the target gene, decreased expres- sion level ofecdysone receptor （LsEcR） gene and caused a higher nymphal mortality rate and delayed development. Ingestion of 20-hydroxyecdysone on LsPhm-dsRNA-exposed nymphs did not increase LsPhm expression level, but almost completely rescued the LsEcR mRNA level, and relieved the negative effects on survival and development. Thus, our data suggest that the putative LsPhm encodes a ftmctional 25-hydroxylase that catalyzes the biosynthesis of ecdysteroids in L. striatellus.

关键词： double-stranded RNA ecdysteroidogenesis Laodelphax striatellus perfor-mance Phantom

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陕西省柞水县北川沟铜矿成矿地质特征及控矿因素与找矿标志

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世界有色金属 2024年第3期 44-46页

作者：黎伟峰王建平刘婉嫔商洛西北有色七一三总队有限公司陕西商洛726000

北川沟铜矿区位于柞(水)-山(阳)泥盆纪热水沉积盆地中部,区域性凤镇-山阳深大断裂北侧,处于柞水-山阳菱铁多金属成矿带中段。此成矿带产于中泥盆统大西沟组地层中、上段中,印支-燕山期强烈的造山运动,形成了一系列近东西向断裂构造,并... 详细信息

北川沟铜矿区位于柞(水)-山(阳)泥盆纪热水沉积盆地中部,区域性凤镇-山阳深大断裂北侧,处于柞水-山阳菱铁多金属成矿带中段。此成矿带产于中泥盆统大西沟组地层中、上段中,印支-燕山期强烈的造山运动,形成了一系列近东西向断裂构造,并使矿源层中成矿物质活化,迁移于有利的构造部位富集形成热水沉积-改造型矿床。本文对北川沟铜矿地质特征、物化探资料进行分析,总结其控矿因素与找矿标志,希望能为今后在本区找矿工作提供借鉴。

关键词：铜矿成矿地质特征控矿因素找矿标志北川沟柞水县陕西省

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单侧与双侧经皮椎体成形术对老年骨质疏松性胸腰椎压缩性骨折患者腰椎功能及疼痛程度的影响对比分析

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四川生理科学杂志 2024年第5期46卷 1112-1114页

作者：卢志斌徐晓辉万平余干县人民医院骨科江西上饶335100

目的:对比单侧与双侧经皮椎体成形术(PVP)治疗老年骨质疏松性胸腰椎压缩性骨折(OVCF)的临床疗效。方法:2020年6月至2023年6月本院行PVP治疗的70例老年OVCF患者,根据穿刺方法分为单侧组(n=36,单侧穿刺行PVP手术)和双侧组(n=34,双侧穿刺行... 详细信息

目的:对比单侧与双侧经皮椎体成形术(PVP)治疗老年骨质疏松性胸腰椎压缩性骨折(OVCF)的临床疗效。方法:2020年6月至2023年6月本院行PVP治疗的70例老年OVCF患者,根据穿刺方法分为单侧组(n=36,单侧穿刺行PVP手术)和双侧组(n=34,双侧穿刺行PVP手术)。比较两组手术相关情况,在术前、术后3m、6m采用ODI和VAS量表评估两组患者腰椎功能及疼痛程度,并统计并发症情况。结果:单侧组手术时间更短、透视次数和骨水泥用量更少(P<0.05)。双侧组术后6mODI、VAS评分低于单侧组(P<0.05)。结论:双侧穿刺行PVP治疗OVCF在缓解疼痛及改善腰椎功能方面更优,但单侧穿刺可缩短手术时间,减少透视次数和骨水泥用量。

关键词：单侧经皮椎体成形术双侧经皮椎体成形术骨质疏松性胸腰椎压缩性骨折疼痛程度

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RNA interference-mediated silencing of a Halloween gene spookier affects nymph performance in the small brown planthopper Laodelphax striatellus

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Insect Science 2015年第2期22卷 191-202页

作者： Shuang Jia pin-Jun wan Li-Tao Zhou Li-Li Mu Guo-Qing Li Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests College of Plant Protection Nanjing AgriculturalUniversity Nanjing China

Post-embryonic development of insects is highly dependent on ecdysteroid hormone 20-hydroxyecdysone. Halloween gene spookier （spok, cyp307a2） has been documented to be involved in ecdysteroidogenesis in Drosophila melanogaster and Bombyx mori. We describe here the cloning and characterization of Halloween gene spookier （Lsspok, Lscyp307a2） in the small brown planthopper Laodelphax striatellus, a hemipteran insect species. LsSPOK has three insect-conserved P450 motifs, that is, Helix-K, PERF motif and heme-binding domain. Temporal and spatial expression patterns of Lsspok were evaluated by quantitative polymerase chain reaction. Through the fouth- instar and the early fifth-instar stages, Lsspok showed two expression peaks in the second- and fifth-day fourth-instar nymphs, and two troughs in the first-day fourth and fifth in- stars. On day 5 of the fourth-instar nymphs, Lsspok clearly had a high transcript level in the thorax where prothoracic glands were located. Dietary introduction of double-stranded RNA of Lsspok in the nymph stage successfully knocked down the target gene, decreased expression level ofecdysone receptor （LsEcR） gene, caused nymphal lethality and delayed development. Ingestion of 20-hydroxyecdysone in Lsspok-dsRNA-exposed nymphs did not increase Lsspok expression level, but almost completely rescued the LsEcR mRNA level and relieved the negative effects on survival and development. Thus, our data suggest that the ecdysteroidogenic pathway is conserved in insects and LsSPOK is responsible for specific steps in ecdysteroidogenesis in L. striatellus.

关键词： development double-stranded RNA ecdysteroidogenesis Laodelphaxstriatellus lethality spookier

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甲维盐与阿维菌素对褐飞虱繁殖力及天敌稻虱缨小蜂控害能力的影响

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昆虫学报 2024年第4期67卷 538-548页

作者：何佳春王康逸 Mubashar HUSSAIN 魏琪万品俊赖凤香王渭霞傅强中国水稻研究所水稻生物育种全国重点实验室杭州311401 重庆市涪陵区经济作物发展中心重庆408000

【目的】评估甲维盐和阿维菌素两种药剂对褐飞虱Nilaparvata lugens繁殖力及其寄生性天敌稻虱缨小蜂Anagrus nilaparvatae的影响,探明两种药剂引起褐飞虱田间再猖獗的原因。【方法】田间调查了使用5%甲氨基阿维菌素苯甲酸盐(甲维盐)微乳... 详细信息

【目的】评估甲维盐和阿维菌素两种药剂对褐飞虱Nilaparvata lugens繁殖力及其寄生性天敌稻虱缨小蜂Anagrus nilaparvatae的影响,探明两种药剂引起褐飞虱田间再猖獗的原因。【方法】田间调查了使用5%甲氨基阿维菌素苯甲酸盐(甲维盐)微乳剂(microemulsion,ME)(用量225 mL/hm^(2))和5%阿维菌素悬浮剂(suspension concentrate,SC)(用量240 mL/hm^(2))后对田间褐飞虱虫口密度和稻虱缨小蜂成蜂寄生率的影响;室内试验采用稻茎浸渍法测定甲维盐和阿维菌素对褐飞虱4龄若虫的毒力以及不同浓度(LC_(15),LC_(30),LC_(50)和LC_(75))甲维盐和阿维菌素对褐飞虱繁殖力及稻虱缨小蜂成蜂存活和寄生能力的影响,同时采用玻璃管药膜法测定了这两种药剂对稻虱缨小蜂成蜂的毒力,评价了其风险级别。【结果】田间调查结果显示,施用5%甲维盐ME或5%阿维菌素SC显著增加了田间褐飞虱虫口密度,显著降低了稻虱缨小蜂成蜂田间寄生率。室内生物测定结果显示甲维盐和阿维菌素对褐飞虱4龄若虫的LC 50值分别为1.20和2.03 mg/L,各浓度(LC_(15)~LC_(75))甲维盐和中低致死浓度(LC_(15)~LC_(50))阿维菌素处理后的褐飞虱成虫单雌产卵量均显著高于对照。甲维盐和阿维菌素对稻虱缨小蜂成蜂的LC_(50)值分别为0.0145和0.0205 mg/L,田间用量的安全系数分别为4.52×10^(-4)~9.05×10^(-4)和8.52×10^(-4)~14.22×10^(-4),均为极高风险性药剂。【结论】甲维盐和阿维菌素会引起田间褐飞虱的再猖獗,主要有两方面的原因,一是刺激褐飞虱雌成虫产卵,二是作为稻虱缨小蜂的高风险药剂,极大地削弱了该蜂对褐飞虱的控害作用。因此,应减少两种药剂在稻田的使用,避免导致褐飞虱的再猖獗。

关键词：褐飞虱稻虱缨小蜂甲维盐阿维菌素再猖獗繁殖力寄生率

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Molecular cloning and characterization of a putative proline dehydrogenase gene in the Colorado potato beetle, Leptinotarsa decemlineata

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Insect Science 2014年第2期21卷 147-158页

作者： pin-Jun wan Dong Lu Wen-Chao Guo Tursun Ahmat Lu Yang Li-Li Mu Guo-Qing Li Education Ministry Key Laboratory of Integrated Management of Crop Diseases and Pests College of Plant Protection Nanjing Agricultural University Nanjing Department of Plant Protection Xinjiang Academy of Agricultural Sciences Urumqi China

Leptinotarsa decemlineata adults exhibit a season-dependent activity. In spring, post-diapause beetles often fly a long distance from overwintering sites to potato fields. In summer and autumn, the flight ability is sharply reduced. Proline is the main energy substrate ofL. decemlineata during flight and proline dehydrogenase （ProDH） catalyzes the first step in proline catabolism. Here we identified a putative LdProDHgene; it had three cDNA isoforms which shared the same 5＇UTR and coding region, but differed in the lengths of 3＇UTRs （515, 1 092 and 1 242 bp for isoforms-1, -2 and -3, respectively）. LdProDH encoded a 616 amino acid protein that showed high sequence similarity to ProDH-like proteins from other insect species. LdProDHwas expressed in the third and fourth instars larvae and adults, but not in pupae. Dietary ingestion of bacterially expressed LdProDH- dsRNA by adults significantly decreased its messenger RNA （mRNA） level, and caused an elevation of free proline content in the hemolymph. Further observation revealed that three canonical polyadenylation signals （AATAAA） were tandemly located in the 3＇UTR of isoform-3. The first, second and third polyadenylation sites gave rise to isoforms-1, -2 and -3, respectively. Analysis of the genomic DNA uncovered that the three isoforms resulted from alternative polyadenylation. The mRNA level of isoform-1, which expressed at low levels in pre-diapause adults, became abundant in post-diapause beetles. It is indicated that the LdProDH expression is fine-tuned through 3＇UTR to control proline catabolism for the season-dependent activity ofL. decemlineata adults.

关键词： alternative polyadenylation isoform RNA interference temporal expres-sion 3~UTR

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Effect of"Natural Polypill",Xuezhikang on Serum Cholesterol Metabolism Markers in Early Menopausal Women with Hypercholesterolemia

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Chinese Journal of Integrative Medicine 2022年第3期28卷 202-207页

作者： FENG Yan LU Shu-li JIN Xiang-gong GAO Jie CHEN wan-pin wanG Yi-xin XU Hao wanG Lu-ya Department of General Medicine Beijing Anzhen HospitalCapital Medical UniversityBeijing(100029)China Department of Cardiology Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing(100091)China

Objective:To analyze the effect of Xuezhikang on the markers of the serum lipid levels of cholesterol synthesis and absorption in early menopausal women with hypercholesterolemia,and preliminarily explore its lipid-lowering ***:A total of 90 early menopausal women with hypercholesterolemia were enrolled from December,2014 to May,2016 from Beijing Anzhen Hospital,Capital Medical University,who were randomly allocated to receive Xuezhikang(1200 mg/d,orally)or atorvastatin(10 mg/d,orally)according to a random number *** levels of some related biomarkers,including cholesterol synthesis markers(squalene,dihydrocholesterol,dehydrocholesterol,and lathosterol),and absorption markers(campesterol,stigmasterol,and sitosterol)as well as safety indices were obtained at baseline and after 8 weeks of the ***:Eight weeks after treatment,both Xuezhikang and atorvastatin significantly reduced the levels of total cholesterol,triglycerides,low density cholesterol compared to baseline(all P0.05),however,a more obvious downward trend was shown in the Xuezhikang *** addition,all the safety indices showed no significant differences between the two *** the creatinekinase level in the Xuezhikang group was significantly higher,it remained within the safe ***:Xuezhikang may have more comprehensive effects on the markers of cholesterol synthesis and metabolism in early menopausal women with hypercholesterolemia through ergosterol and flavonoids in its"natural polypill."

关键词： early menopausal women hypercholesterolemia Xuezhikang cholesterol metabolism markers atorvastatin

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Hypoxia signaling in human health and diseases:implications and prospects for therapeutics

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Signal Transduction and Targeted Therapy 2022年第8期7卷 2787-2816页

作者： Zhen Luo Mingfu Tian Ge Yang Qiaoru Tan Yubing Chen Geng Li Qiwei Zhang Yongkui Li pin wan Jianguo Wu Guangdong Provincial Key Laboratory of Virology Institute of Medical MicrobiologyJinan UniversityGuangzhou 510632China Foshan Institute of Medical Microbiology Foshan 528315China The First Affiliated Hospital of Jinan University Guangzhou 510632China The Affiliated Shunde Hospital of Jinan University Foshan 528303China

Molecular oxygen(O2)is essential for most biological reactions in mammalian *** the intracellular oxygen content decreases,it is called *** process of hypoxia is linked to several biological processes,including pathogenic microbe infection,metabolic adaptation,cancer,acute and chronic diseases,and other stress *** mechanism underlying cells respond to oxygen changes to mediate subsequent signal response is the central question during ***-inducible factors(HIFs)sense hypoxia to regulate the expressions of a series of downstream genes expression,which participate in multiple processes including cell metabolism,cell growth/death,cell proliferation,glycolysis,immune response,microbe infection,tumorigenesis,and ***,hypoxia signaling also interacts with other cellular pathways,such as phosphoinositide 3-kinase(PI3K)-mammalian target of rapamycin(mTOR)signaling,nuclear factor kappa-B(NF-κB)pathway,extracellular signal-regulated kinases(ERK)signaling,and endoplasmic reticulum(ER)*** paper systematically reviews the mechanisms of hypoxia signaling activation,the control of HIF signaling,and the function of HIF signaling in human health and *** addition,the therapeutic targets involved in HIF signaling to balance health and diseases are summarized and highlighted,which would provide novel strategies for the design and development of therapeutic drugs.

关键词： diseases metabolism drugs

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SARS-CoV-2 Nsp8 induces mitophagy by damaging mitochondria

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Virologica Sinica 2023年第4期38卷 520-530页

作者： Shan Zong Yan Wu Weiling Li Qiang You Qian Peng Chenghai wang pin wan Tao Bai Yanling Ma Binlian Sun Jialu Qiao Wuhan Institute of Biomedical Sciences School of MedicineJianghan UniversityWuhan430056China State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan430071China Hubei Key Laboratory of Wudang Local Chinese Medicine Research Hubei University of MedicineShiyan442000China Division of Gastroenterology Tongji Medical CollegeHua Zhong University of Science and TechnologyWuhan430030China Department of Respiratory and Critical Care Medicine Hubei Province Clinical Research Center for Major Respiratory DiseasesNHC Key Laboratory of Pulmonary DiseasesUnion HospitalTongji Medical CollegeHua Zhong University of Science and TechnologyWuhan430030China

Autophagy plays an important role in the interaction between viruses and host ***-CoV-2 infection can disrupt the autophagy process in target ***,the precise molecular mechanism is still *** this study,we discovered that the Nsp8 of SARS-CoV-2 could cause an increasing accumulation of autophagosomes by preventing the fusion of autophagosomes and *** further investigation,we found that Nsp8 was present on mitochondria and can damage mitochondria to initiate *** results of experiments with immunofluorescence revealed that Nsp8 induced incomplete ***,both domains of Nsp8 orchestrated their function during Nsp8-induced mitophagy,in which the N-terminal domain colocalized with mitochondria and the C-terminal domain induced auto/*** novel finding expands our understanding of the function of Nsp8 in promoting mitochondrial damage and inducing incomplete mitophagy,which helps us to understand the etiology of COVID-19 as well as open up new pathways for creating SARS-CoV-2 treatment methods.

关键词： SARS-CoV-2 Nsp8 Autophagy Mitophagy Mitochondrial damage

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带药移栽对水稻害虫防控的减药增效作用分析

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昆虫学报 2024年第4期67卷 477-489页

作者：魏琪张明朱旭晖何佳春刘连盟赖凤香王渭霞万品俊刘龙生傅强中国水稻研究所水稻生物育种全国重点实验室杭州311401 衡阳市农业科学院衡阳421101

【目的】以新型、高效、持效期长的杀虫剂三氟苯嘧啶和四唑虫酰胺为例,探究带药移栽对水稻害虫的防控效果及农药减量增效的实践意义。【方法】通过室内模拟秧苗带药移栽分别测定了1.95,5.85和9.75 mg a.i./m^(2)三氟苯嘧啶处理对褐飞虱N... 详细信息

【目的】以新型、高效、持效期长的杀虫剂三氟苯嘧啶和四唑虫酰胺为例,探究带药移栽对水稻害虫的防控效果及农药减量增效的实践意义。【方法】通过室内模拟秧苗带药移栽分别测定了1.95,5.85和9.75 mg a.i./m^(2)三氟苯嘧啶处理对褐飞虱Nilaparvata lugens和白背飞虱Sogatella furcifera成虫或若虫的毒力和持效期,并在浙江杭州和湖南衡阳两地评估了早、中、晚稻秧苗高剂量带药移栽处理对二化螟Chilo suppressalis和稻飞虱的田间防效以及不同施药模式对稻田寄生蜂种群动态的影响。【结果】室内经5.85和9.75 mg a.i./m^(2)三氟苯嘧啶处理的秧苗在移栽后35 d时使褐飞虱雌成虫和3龄若虫均能保持75%以上的校正死亡率,且5.85 mg a.i./m^(2)剂量处理在移栽后28 d时使白背飞虱雌成虫的校正死亡率大于80%;使用12.75和25.50 mg a.i./m^(2)四唑虫酰胺处理秧苗在移栽后35 d时对早稻二化螟为害的校正防效均在85%以上。综合浙江杭州和湖南衡阳两地中、晚稻田间试验结果表明,应用5.85 mg a.i./m^(2)三氟苯嘧啶和25.50 mg a.i./m^(2)四唑虫酰胺进行秧苗“送嫁药”处理可减少1~2次大田用药和98%以上的农药有效成分用量,同时还保持着与常规喷施相当的防效,并使寄生蜂总种类和总数量分别提高了29.0%~49.7%和61.2%~69.3%。【结论】秧苗移栽前施用防效优良且持效期长的杀虫剂能减少水稻前期的施药次数和用量,产生较好的经济和生态效益。本研究为利用带药移栽技术推行水稻病虫害的绿色防控提供了重要参考。

关键词：带药移栽水稻害虫三氟苯嘧啶四唑虫酰胺农药减量天敌保护

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