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A modular microfluidic system based on a multilayered configuration to generate large-scale perfusable microvascular networks

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Microsystems & nanoengineering 2021年第1期7卷 63-75页

作者： Tao Yue Da Zhao Duc T.T.phan Xiaolin Wang Joshua Jonghyun Park Zayn Biviji Christopher C.W.Hughes Abraham P.Lee Department of Biomedical Engineering University of CaliforniaIrvineCAUSA School of Mechatronic Engineering and Automation Shanghai UniversityShanghaiChina Department of Molecular Biology and Biochemistry University of CaliforniaIrvineCAUSA Department of Micro/nano Electronics Shanghai Jiao Tong UniversityShanghaiChina national key Laboratory of Science and Technology for Micro/nano Fabrication Shanghai Jiao Tong UniversityShanghaiChina key Laboratory for Thin Film and Micro Fabrication of the Ministry of Education Shanghai Jiao Tong UniversityShanghaiChina Department of Electrical Engineering and Computer Science University of CaliforniaIrvineCAUSA. Department of Applied Mathematics-Biology Brown UniversityProvidenceRIUSA. Department of Mechanical and Aerospace Engineering University of CaliforniaIrvineCAUSA

The vascular network of the circulatory system plays a vital role in maintaining homeostasis in the human *** this paper,a novel modular microfluidic system with a vertical two-layered configuration is developed to generate largescale perfused microvascular networks in *** two-layer polydimethylsiloxane(PDMS)configuration allows the tissue chambers and medium channels not only to be designed and fabricated independently but also to be aligned and bonded *** method can produce a modular microfluidic system that has high flexibility and scalability to design an integrated platform with multiple perfused vascularized tissues with high *** medium channel was designed with a rhombic shape and fabricated to be semiclosed to form a capillary burst valve in the vertical direction,serving as the interface between the medium channels and tissue *** and anastomosis at the vertical interface were successfully achieved by using different combinations of tissue chambers and medium *** large-scale microvascular networks were generated and quantified in terms of vessel length and *** leakage of the perfused 70-kDa FITC-dextran confirmed the lumenization of the microvascular networks and the formation of tight vertical interconnections between the microvascular networks and medium channels in different structural *** platform enables the culturing of interconnected,large-scale perfused vascularized tissue networks with high density and scalability for a wide range of multiorgan-on-a-chip applications,including basic biological studies and drug screening.

关键词： interconnection networks configuration

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Early Detection of QoE Deterioration in HTTP Adaptive Streaming

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Journal of Computer and Communications 2017年第14期5卷 14-27页

作者： phan Xuan-Tan Eiji kamioka Graduate School of Engineering and Science Shibaura Institute of Technology Tokyo Japan

Apart from video rate (or requested bitrate), Mean Opinion Score (MOS) has increasingly become a primary term representing Quality of Experience (QoE) in HTTP adaptive streaming (HAS). By monitoring this metric, QoE management can effectively maximize QoE for the users. However, due to various behaviors of particular commercial HAS players, deciding an appropriate monitoring interval has not been fully investigated yet. In this paper, an optimal interval is proposed to be equal to duration of a video chunk in order to aid service managers in early detecting QoE deterioration and limiting the probability of video rate deterioration. The optimal monitoring interval is evaluated by comparing with other values of interval in terms of ratio of video rate deterioration. Furthermore, MOS-based QoE monitoring method which takes into account the proposed interval is thus compared with video rate based monitoring method. The results show that with optimal interval, MOS monitoring guarantees a low ratio of video rate deterioration (around 10% for buffering state and 40% for steady state) and small average CPU Load (about 11.45%).

关键词： QoE MOS QoS Video Rate Detection Time Optimal Monitoring Interval

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PGRL1 and LHCSR3 Compensate for Each Other in Controlling Photosynthesis and Avoiding Photosystem I Photoinhibition during High Light Acclimation of Chlamydomonas Cells

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Molecular Plant 2017年第1期10卷 216-218页

作者： Frédéric Chaux Xenie Johnson Pascaline Auroy Audrey Beyly-Adriano Isabelle Te Stéphan Cuiné Gilles Peltier CEA CNRS Aix-Marseille Université Institut de Biosciences et Biotechnologies Aix-Marseille UMR 7265 Laboratoire de Bioénergétique et Biotechnologie des Bactéries et Microalgues CEA Cadarache Saint-Paul-lez-Durance 13108 France

Dear Editor,In natural environments, photosynthetic organisms experience frequent changes in the light supply, requiring modulation of light harvesting and electron transfer reactions to avoid mismatch between light conversion and metabolic reactions that may result in the production of harmful reactive oxygen species. They have thus evolved photoprotective regulatory mechanisms including the dissipation of excess energy （or non-photochemical quench-ing, nPQ）,

关键词： Controlling Photoinhibition Chlamydomonas

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Balanced nutrition Practiced with Limitations during the COVID-19 Pandemic: The Experience of Teachers in Subang District, West Java, Indonesia

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Open Journal of Preventive Medicine 2022年第1期12卷 10-29页

作者： Avita Aliza Usfar Ju Lan phan Dakra Consultant Jakarta Indonesia

The purpose of the study is to assess the knowledge on Balanced nutrition and its practices among pre-school teachers in Subang district, West Java, Indonesia before and during the COVID-19 pandemic (before March 2020 vs. October 2021) and to seek differences in the knowledge and practices of teachers who attended nutrition training and those who did not attend any nutrition training. We evaluated the knowledge and practice of 142 teachers based on 10 messages of the 2014 Indonesian Balanced nutrition Guidelines, using google online survey platform. The top three messages mostly selected by the teachers were consuming a variety of staple foods (87%), drinking adequate and safe water (87%), and eating plenty of vegetables and fruits (86%), while the least selected were enjoying a variety of foods (41%) and reading food labels (28%). A slight increase was found in teachers who consumed fruits daily, while the practice of washing hands before and during the pandemic was statistically and significantly increased (58% to 72%;p nd in teachers who consumed vegetables and animal protein, as well as performed physical exercise daily. Majority of teachers (80%) participated in some kinds of nutrition training in the last four years. The top three gaps of knowledge between trained and non-trained teachers were on physical activity and maintaining ideal body weight (64% vs. 33%;statistically significant at p ntake of sweet, salty, and fatty food;and consuming high protein food, with the range between 20 to 35 percentage points. We concluded that teachers acquired knowledge on nutrition to some degree, but its application faced limitations during the pandemic. Dissemination of Balanced nutrition should continue.

关键词： Balanced nutrition knowledge Practice Training Indonesia

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Optimization of High-Performance Liquid Chromatography Parameters for Purification of Oligonucleotide-A

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American Journal of Analytical Chemistry 2022年第2期13卷 39-50页

作者： Christina Perez Mubeen Rani Tuan phan Department of Chemistry Texas Southern University Houston USA

The modification of high-performance liquid chromatography parameters leads to a more effective oligonucleotide-A purification process. Using various experimental parameters such as buffer, concentration, and pH, a method for optimizing the purification of an oligonucleotide-A on a reverse-phase C18 column was created. To purify oligonucleotide-A, High-Performance Liquid Chromatography (HPLC), Ultraviolet-Visible Spectrophotometry (UV-Vis), Liquid Chromatography-Mass Spectrometry (LC-MS), and lyophilization were used. Chromatographic data were collected with a semi-prep HPLC system, quantified with the UV-Vis technique, and validated with the LC-MS method. The most optimized parameters found to obtain the purity of 93.0% are 40 mM triethylammonium bicarbonate (TEAB) buffer with pH 7, which is approximately 6.0% higher than the reported method of which the purity is 87.0%. However, the yield under these conditions was reduced by about 5%. The worst possible optimized settings that resulted in the lowest purity (84.0%) and yield (69.0%) are 10 mM ammonium acetate (nH4CH3CO2) with pH 7.

关键词： Purification High-Performance Liquid Chromatography Oligonucleotide-A Dipotassium Phosphate Triethylammonium Acetate Triethylammonium Bicarbonate Ammonium Acetate

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Rapid and efficient characterization of cervical collagen orientation using linearly polarized colposcopic images

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Journal of Innovative Optical Health Sciences 2023年第5期16卷 21-31页

作者： Cat phan ngoc khuong Dung nguyen Huu Quang Hai Pham Thanh Long nguyen Quynh nguyen ngoc Duc Le Huynh Tien Tran Van Tu Ly Anh Department of Applied Physics Ho Chi Minh City University of Technology 268 Ly Thuong kiet Street District 10 Ho Chi Minh City 700000Vietnam Quality Management Department Tu Du Hospital 284 Cong QuynhDistrict 1 Ho Chi Minh City 700000Vietnam Department of Biomedical Engineering nguyen Tat Thanh University 300A Nguyen Tat Thanh District 4Ho Chi Minh City 700000Vietnam Faculty of Science and Technology Jean Monnet University 11 Rue du Docteur Annino Saint Etienne 42000France

Collagen provides tissue strength and structural *** fication of the orientated dispersion of collagen fibers is an important factor when studying the mechanical properties of the *** this study,for the first time,a new method for rapid characterization of the collagen fiber orientations of the cervix using linearly polarized light colposcopy is presented.A total of 24 colposcopic images were captured using a cross-polarized imaging system with white LED light *** the preprocessing stage,the Red channel of the RGB image was chosen,which contains no information of the blood vessels because of the low-absorption of blood cells in the red ***,which is an ImageJ plug-in,was used to estimate the local orientation of the collagen *** result shows that in the nonpregnant cervix,the middle zone(Zone 2)has circumferentially aligned collagen fibers while the inner zone(Zone 1)has randomly *** collagen fiber dispersion in Zone 2 is much smaller than that in Zone 1 at all four quadrants region(anterior,posterior,left,and right quadrant).This new analysis technique could potentially combine with diagnostic tools to provide a quantitative platform of collagen fibers in the clinic.

关键词： Cervix collagen fibers collagen orientation coherency cross-polarized imaging colposcopy

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Strigolactones interact with other phytohormones to modulate plant root growth and development

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The Crop Journal 2022年第6期10卷 1517-1527页

作者： Huwei Sun Weiqiang Li David J.Burritt Hongtao Tian Heng Zhang Xiaohan Liang Yuchen Miao Mohammad Golam Mostofa Lam-Son phan Tran Laboratory of Rice Biology in Henan Province Collaborative Innovation Center of Henan Grain CropsCollege of AgronomyHenan Agricultural UniversityZhengzhou 450046HenanChina Jilin Da’an Agro-ecosystem national Observation Research Station Changchun Jingyuetan Remote Sensing Experiment StationKey Laboratory of Mollisols AgroecologyNortheast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130102JilinChina State key Laboratory of Cotton Biology Henan Joint International Laboratory for Crop Multi-Omics ResearchSchool of Life SciencesHenan UniversityKaifeng 475001HenanChina d Department of Botany University of OtagoDunedin 9054New Zealand Institute of Genomics for Crop Abiotic Stress Tolerance Department of Plant and Soil ScienceTexas Tech UniversityLubbockTX 79409USA Institute of Research and Development Duy Tan University03 Quang TrungDa Nang 550000Vietnam

Strigolactones(SLs),which are biosynthesized mainly in roots,modulate various aspects of plant growth and ***,we review recent research on the role of SLs and their cross-regulation with auxin,cytokinin,and ethylene in the modulation of root growth and *** nutrientsufficient conditions,SLs regulate the elongation of primary roots and inhibit adventitious root formation in eudicot *** promote the elongation of seminal roots and increase the number of adventitious roots in grass plants in the short term,while inhibiting lateral root development in both grass and eudicot *** effects of SLs on the elongation of root hairs are variable and depend on plant species,growth conditions,and SL *** or phosphate deficiency induces the accumulation of endogenous SLs,modulates root growth and *** analyses indicate cross-regulation of SLs with auxin,cytokinin,and ethylene in regulation of root growth and *** discuss the implications of these studies and consider their potential for exploiting the components of SL signaling for the design of crop plants with more efficient soil-resource utilization.

关键词： Cross-regulation Development Phytohormones Roots Signaling Strigolactones

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Collaborative Approach Using Psychophysiology and Psychophysics for Optimal Threshold Determination in HAS Service QoE Management

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Journal of Computer and Communications 2018年第8期6卷 57-81页

作者： phan Xuan Tan Eiji kamioka Graduate School of Engineering and Science Shibaura Institute of Technology Tokyo Japan

In HTTP adaptive streaming (HAS) services, Quality of Experience (QoE) management has increasingly played an important role in guaranteeing the expected video quality perceived by the users with the minimal network resource. To achieve this, QoE must be automatically monitored and accurately controlled when it falls to a specific threshold. However, how to determine the QoE threshold with a scientific basis has not been carefully studied yet. In this paper, a new method to determine a QoE threshold based on biological information is proposed for a QoE management in HAS services. More concretely, the QoE threshold is determined by modeling the impact of the stimulus on the biological information obtained from the users who are watching videos. As the biological information, Skin Conductance and Heart Rate associated with Autonomic nervous System (AnS) have been investigated. The evaluation results demonstrate that the QoE management by using the determined threshold can save more than 4.855% of the bandwidth consumption per control ensuring as high video quality as by using the fair threshold.

关键词： QoE HTTP Adaptive Streaming Biological Information Weber-Fechner Law

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奢华酒店业服务创新成功决定因素研究:基于巴黎雅典娜广场酒店案例

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研究与发展管理 2012年第5期24卷 34-41页

作者：卢晓 phan M C T 复旦大学管理学院上海200433 法国高等经济与商业学院巴黎95021

坐落于巴黎的雅典娜广场酒店以其服务不断创新一直受到国际服务业同行和消费者高度肯定.本文以服务创新理论为框架,通过案例研究,对酒店高层管理人员、普通工作人员、供应商和客人进行一系列深入访谈,揭示了奢华酒店服务创新成功的5个... 详细信息

坐落于巴黎的雅典娜广场酒店以其服务不断创新一直受到国际服务业同行和消费者高度肯定.本文以服务创新理论为框架,通过案例研究,对酒店高层管理人员、普通工作人员、供应商和客人进行一系列深入访谈,揭示了奢华酒店服务创新成功的5个决定因素:植根在服务业企业文化中的创新战略、具有超强执行力和魅力的高级管理团队、合理支持体系和组织结构、直接和开放的沟通渠道以及积极配合的员工"大家庭".其中,积极配合的员工"大家庭"在奢华酒店服务创新中起到关键作用,是传统服务创新理论中没有涉及的新要素,是本研究的理论贡献和创新之处.

关键词：奢华酒店业服务创新案例研究

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High-flow nasal oxygen availability for sedation decreases the use of general anesthesia during endoscopic retrograde cholangiopancreatography and endoscopic ultrasound

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World Journal of Gastroenterology 2016年第47期22卷 10398-10405页

作者： Roman Schumann nikola S natov klifford A Rocuts-Martinez Matthew D Finkelman Tom V phan Sanjay R Hegde Robert M knapp Department of Anesthesiology and Perioperative Medicine Tufts Medical CenterBoston MA 02111United States The Gastroenterology/Hepatology Division Tufts Medical CenterBoston MA 02111United States Critical Care Medicine Dartmouth-Hitchcock Medical CenterLebanon NH 03756 United States Tufts University School of Dental Medicine Boston MA 02111United States Tufts University School of Medicine Boston MA 02111United States

AIM To examine whether high-flow nasal oxygen(HFnO) availability influences the use of general anesthesia(GA) in patients undergoing endoscopic retrograde cholangiopancreatography(ERCP) and endoscopic ultrasound(EUS) and associated *** In this retrospective study, patients were stratified into 3 eras between October 1, 2013 and June 30, 2014 based on HFnO availability for deep sedation at the time of their endoscopy. During the first and last 3-mo eras(era 1 and 3), no HFnO was available, whereas it was an option during the second 3-mo era(era 2). The primary outcome was the percent utilization of GA vs deep sedation in each period. Secondary outcomes included oxygen saturation nadir during sedation between periods, as well as procedure duration, and anesthesia-only time between periods and for GA vs sedation cases *** During the study period 238 ERCP or EUS cases were identified for analysis. Statistical testing was employed and a P nificant unless the Bonferroni correction for multiple comparisons was used. General anesthesia use was significantly lower in era 2 compared to era 1 with the same trend between era 2 and 3(P = 0.012 and 0.045 respectively). The oxygen saturation nadir during sedation was significantly higher in era 2 compared to era 3(P not between eras 1 and 2(P = 0.028) or 1 and 3(P = 0.069). The procedure time within each era was significantly longer under GA compared to deep sedation(P ≤ 0.007) as was the anesthesia-only time(P ≤ 0.001).COnCLUSIOn High-flow nasal oxygen availability was associated with decreased GA utilization and improved oxygenation for ERCP and EUS during sedation.

关键词： Endoscopic ultrasound Endoscopic retrograde cholangiopancreatography Endoscopy Sedation Anesthesia Oxygenation High flow nasal oxygen

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