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Cell-type-specific transcriptomics reveals that root hairs and endodermal barriers play important roles in beneficial plant-rhizobacterium interactions

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Molecular Plant 2023年第7期16卷 1160-1177页

作者： Eline H.Verbon Louisa M.Liberman Jiayu Zhou Jie Yin CornéM.J.Pieterse philip n.benfey loannis A.Stringlis Ronnie de Jonge Plant-Microbe Interactions Department of BiologyScience4LifeUtrecht UniversityP.0.Box 800.563508 TB Utrechtthe Netherlands Howard Hughes Medical Institute Duke UniversityDurhamNC 27708USA Department of Biology Duke UniversityDurhamNC 27708USA Laboratory of Plant Pathology Agricultural University of Athens75 lera Odos str11855 AthensGreece

Growth-and health-promoting bacteria can boost crop productivity in a sustainable *** simiae WCS417is sucha bacterium that efficiently colonizes roots,modifiesthe architecture of the root systemto increase its size,and induces systemic resistance to make plants more resistant to pests and *** previous work suggested that WCS417-induced phenotypes are controlled by root cell-type-specific ***,it remains unclear how WCS417 affects these *** this study,we transcriptionally profiled five Arabidopsis thaliana root cell types following WCS417 *** found that the cortex and endodermis have the most differentially expressed genes,even though they are not in direct contact with this epiphytic *** of these genes are associated with reduced cell wall biogenesis,and mutant analysis suggests that this downregulation facilitates WCS417-driven root architectural ***,we observed elevated expression of suberin biosynthesis genes and increased deposition of suberin in the endodermis of WCS417-colonized *** an endodermal barrier mutant,we showed the importance of endodermal barrier integrity for optimal plant-beneficial bacterium *** of the transcriptome profiles in the two epidermal cell types that are in direct contact with WcS417-trichoblasts that form root hairs and atrichoblasts that do not-implies a difference in potential for defense gene *** both cell types respond to WCS417,trichoblasts displayed both higher basal and WCS417-dependent activation of defense-related genes compared with *** suggests that root hairs may activate root immunity,a hypothesis that is supported by differential immune responses in root hair *** together,these results highlight the strength of cell-type-specific transcriptional profiling to uncover"masked"biological mechanisms underlying beneficial plant-microbe associations.

关键词： FACs cell-type-specific transcriptomics root immunity beneficial rhizobacteria suberin root hair

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Development Rooted in Interwoven networks

Development Rooted in Interwoven Networks

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第十二届全国植物基因组学大会

作者： philip n.benfey Biology Department and IGSP Center for Systems Biology Duke University and GrassRoots BiotechnologyDurhamNC USA

Specification and maintenance of cell identity are central processes of *** an effort to understand the regulatory networks that control cell identity,we have profiled all cell types and developmental stages within the Arabidopsis *** are using experimental methods to identify networks functioning within different cell types and developmental stages under normal laboratory conditions and under abiotic and biotic *** roots form as repeating units along the root primary axis,however the

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High-repetition-rate source of nanosecond duration kA-current pulses driven by relativistic laser pulses

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High Power Laser Science and Engineering 2024年第3期12卷 106-115页

作者： Michael Ehret Jakub Cikhardt philip Wykeham Bradford Iuliana-Mariana Vladisavlevici Tomas Burian Diego de Luis Jose Luis Henares Rubén Hernández Martín Jon Imanol Apiñaniz Roberto Lera JoséAntonio Pérez-Hernández João Jorge Santos Giancarlo Gatti Centro de Láseres Pulsados(CLPU) VillamayorSpain Czech Technical University in Prague Faculty of Electrical EngineeringPragueCzech Republic Univ.Bordeaux-CnRS-CEA Centre Lasers Intenses et Applications(CELIA)UMR 5107TalenceFrance Department of Radiation and Chemical Physics FZU-Institute of Physics of the Czech Academy of SciencesPragueCzech Republic

We report the first high-repetition-rate generation and simultaneous characterization of nanosecond-scale return currents of kA-magnitude issued by the polarization of a target irradiated with a PW-class high-repetition-rate titanium:sapphire laser system at relativistic *** present experimental results obtained with the VEGA-3 laser at intensities from5×10^(18)to 1.3×10^(20)W cm^(-2).A non-invasive inductive return-current monitor is adopted to measure the derivative of return currents of the order of kA ns-1and analysis methodology is developed to derive return *** compare the current for copper,aluminium and Kapton targets at different laser *** data show the stable production of current peaks and clear prospects for the tailoring of the pulse shape,which is promising for future applications in highenergy-density science,for example,electromagnetic interference stress tests,high-voltage pulse response measurements and charged particle beam *** compare the target discharge of the order of hundreds of nC with theoretical predictions and a good agreement is found.

关键词： current pulses electromagnetic pulse application high-power laser relativistic laser plasma

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The association of diet quality and physical activity with cardiovascular disease and mortality in 85,545 older Australians:A longitudinal study

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Journal of Sport and Health Science 2024年第6期13卷 841-850页

作者： Ding Ding Joe Van Buskirk Stephanie Partridge philip Clare Edward Giovannucci Adrian Bauman nicole Freene Robyn Gallagher Binh nguyen Sydney School of Public Health Faculty of Medicine and Healththe University of SydneyCamperdownNSW 2006Australia Charles Perkins Centre the University of SydneyCamperdownNSW 2006Australia Sydney Local Health District CamperdownNSW 2050Australia School of Health Sciences Faculty of Medicine and Healththe University of SydneyCamperdownNSW 2006Australia national Drug and Alcohol Research Centre UNSW SydneySydneyNSW 2052Australia Departments of nutrition and Epidemiology Harvard T.H.Chan School of Public HealthBostonMA 02115USA Faculty of Health University of CanberraCanberraACT 2617Australia Susan Wakil School of nursing and Midwifery the University of SydneyCamperdownNSW 2050Australia

Background:A quality diet and an active lifestyle are both important cornerstones of cardiovascular disease(CVD)***,despite their interlinked effects on metabolic health,the 2 behaviors are rarely considered jointly,particularly within the context of CVD *** examined the independent,interactive,and joint associations of diet and physical activity with CVD hospitalization,CVD mortality,and all-cause ***:CVD-free Australian participants aged 4574 years(n=85,545)reported physical activity,diet,sociodemographic,and lifestyle characteristics at baseline(20062009)and follow-up(20122015),and data were linked to hospitalization and death registries(03/31/2019 for CVD hospitalization and all-cause mortality and 12/08/2017 for CVD mortality).Diet quality was categorized as low,medium,and high based on meeting dietary *** activity was operationalized as(a)total moderate-to-vigorous physical activity(MVPA)as per guidelines,and(b)the composition of MVPA as the ratio of vigorous-intensity physical activity(VPA)to total *** used a left-truncated cause-specific Cox proportional hazards model using time-varying ***:During a median of 10.7 years of follow-up,6576 participants were admitted to the hospital for CVD and 6581 died from all causes(876 from CVD during 9.3 years).A high-quality diet was associated with a 17%lower risk of all-cause mortality than a low-quality diet,and the highest MVPA category(compared with the lowest)was associated with a 44%and 48%lower risk of CVD and all-cause mortality,*** interactions between diet and physical activity were *** all outcomes,the lowest risk combinations involved a high-quality diet and the highest MVPA *** for total MVPA,some VPA was associated with further risk reduction of CVD hospitalization and all-cause ***:For CVD prevention and longevity,one should adhere to both a healthy d

关键词： Diet Physical activity Cardiovascular disease Mortality Cohort studies

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Prehospital transdermal glyceryl trinitrate for ultra- acute ischaemic stroke: data from the RIGHT- 2 randomised sham- controlled ambulance trial

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Stroke & Vascular neurology 2024年第1期9卷 38-49,I0035-I0046页

作者： Jason philip Appleton Lisa J Woodhouse Craig S Anderson Sandeep Ankolekar Lesley Cala Mark Dixon Timothy J England Kailash Krishnan Grant Mair Keith W Muir John Potter Christopher I Price Marc Randall Thompson G Robinson Christine Roffe Else C Sandset Jeffrey L Saver Angela Shone Aloysius niroshan Siriwardena Joanna M Wardlaw nikola Sprigg philip M Bath Stroke University Hospitals Birmingham NHS Foundation TrustBirminghamUK Institute of Applied HealthResearch University of Birmingham College of Medical and Dental SciencesBirminghamUK Stroke Trials Unit Mental Health and Clinical NeurosciencesUniversity of NottinghamNottinghamUK Faculty of Medicine The George Institute for Global HealthSydneyNew South WalesAustralia The George Institute China at Peking University Health Science Center BeijingChina neurology Royal Prince Alfred HospitalSydney Health PartnersSydneyNew South WalesAustralia Department of neurology King's College Hospital NHS Foundation TrustLondonUK Faculty of Health and Medical Sciences University of Western AustraliaCrawleyWestern AustraliaAustralia East Midlands Ambulance Service nHS Trust NottinghamUK Stroke Queen's Medical CentreNottingham University Hospitals NHS TrustNottinghamUK Centre for Clinical Brain Sciences Dementia Research InstituteUniveristy of EdinburghEdinburghUK Institute of neurology and Psychology University of GlasgowGlasgowUK Bob Champion Research and Education Building University of East AngliaNorwichUK Institute of neuroscience Newcastle UniversityNewcastle upon TyneUK Department of neurology Leeds Teaching Hospitals NHS TrustLeedsUK Department of Cardiovascular Sciences and nIHR Leicester Biomedical Research Centre University of LeicesterLeicesterUK Stroke Research in Stoke Institute for Science and Technology in MedicineKeele UniversityStoke-on TrentUK Department of neurology Oslo University HospitalOsloNorway Research and Development Norwegian Air Ambulance FoundationOsloNorway Department of neurology and Comprehensive Stroke Center David Geffen School of MedicineUCLALos AngelesCaliforniaUSA Research and Graduate Services University of NottinghamNottinghamUK Community and Health Research Unit University of LincolnLincolnUK

Background The effect of transdermal glyceryl trinitrate(GTn,a nitrovasodilator)on clinical outcome when administered before hospital admission in suspected stroke patients is ***,we assess the safety and efficacy of GTn in the prespecified subgroup of patients who had an ischaemic stroke within the Rapid Intervention with Glyceryl trinitrate in Hypertensive stroke Trial-2(RIGHT-2).Methods RIGHT-2 was an ambulance-based multicentre sham-controlled blinded-endpoint study with patients randomised within 4hours of *** primary outcome was a shift in scores on the modified Rankin scale(mRS)at day *** outcomes included death;a global analysis(Wei-Lachin test)containing Barthel Index,EuroQol-5D,mRS,telephone interview for cognitive status-modified and Zung depression scale;and neuroimaging-determined‘brain frailty’*** were reported as n(%),mean(SD),median[IQR],adjusted common OR(acOR),mean difference or Mann-Whitney difference(MWD)with 95%*** 597 of 1149(52%)patients had a final diagnosis of ischaemic stroke;age 75(12)years,premorbid mRS>2107(18%),Glasgow Coma Scale 14(2)and time from onset to randomisation 67[45,108]***‘brain frailty’was common:median score 2[2,3](range 0–3).At day 90,GTn did not influence the primary outcome(acOR for increased disability 1.15,95%CI 0.85 to 1.54),death or global analysis(MWD 0.00,95%CI-0.10 to 0.09).In subgroup analyses,there were non-significant interactions suggesting GTn may be associated with more death and dependency in participants randomised within 1hour of symptom onset and in those with more severe *** In patients who had an ischaemic stroke,ultra-acute administration of transdermal GTn in the ambulance did not improve clinical outcomes in a population with more clinical and radiological frailty than seen in previous in-hospital *** IS ALREADY KnOWn On THIS TOPIC⇒Transdermal glyceryl trinitrate(GTn)was associat-ed with less death and dependency in those w

关键词： Glasgow nitrate random

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Structural Controls Analysis and Its Correlation with Geothermal Occurrence at Barrier Volcanic Complex (BVC), Turkana, Kenya

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International Journal of Geosciences 2024年第3期15卷 231-245页

作者： philip Lomorukai Emekwi nicholas O. Mariita Patrick Chege Kariuki Geothermal Training and Research Institute (GeTRI) Dedan Kimathi University of Technology Nyeri Kenya

Geothermal is a clean energy source that is freely available in the subsurface. The exploitation of this vital resource needs intensive exploration in order to identify and quantify its occurrence. The three parameters considered when assessing the viability of a geothermal system include;heat source, fractures and fluids. Geological structures are important in transportation of fluids to and from the heat source aiding in recharge of the geothermal system and enhancing productivity. Remote sensing method was applied in mapping the structures at Barrier Volcanic Complex (BVC) by using hill shading technique which utilized four illumination angles of the sun (azimuth) i.e. 45°, 90°, 150°, and 315°, constant elevation of 45° and exaggeration of 10. The data used was Shuttle Radar Topographic Mission (SRTM) Satellite Imagery. ArcGIS Software was used for lineaments delineation and density mapping, PCI Geomatica was used to generate major faults, while Georose and Rockworks 17 were used to generate the rose diagrams. Geological structural analysis was done by delineating lineaments, determining the density distribution of lineaments and finally determining the structural trends of lineaments. The generated major faults in the area and the location of the occurrence of surface manifestations were compared with the generated lineaments. A total of 260 lineaments were generated whereby at 45° there was a total of 60 lineaments, at 90° 95 lineaments, at 150° 61 lineaments, and at 315° 44 lineaments. The results of structural analysis in the area as shown by the rose diagrams indicate an nnE-SSW and n-S trending of structures. In conclusion, the study area is highly fractured as indicated by the presence of numerous lineaments. These lineaments provide good recharge to the geothermal system and enhance the geothermal reservoir in the area.

关键词： Geothermal Resource Lineaments Lineaments Density Structural Controls

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Unveiling a novel metal-to-metal transition in LuH_(2):Critically challenging superconductivity claims in lutetium hydrides

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Matter and Radiation at Extremes 2024年第3期9卷 65-73页

作者： Dong Wang ningning Wang Caoshun Zhang Chunsheng Xia Weicheng Guo Xia Yin Kejun Bu Takeshi nakagawa Jianbo Zhang Federico Gorelli philip Dalladay-Simpson Thomas Meier Xujie Lü Liling Sun Jinguang Cheng Qiaoshi Zeng Yang Ding Ho-kwang Mao Center for High-Pressure Science and Technology Advanced Research Beijing 100094China Beijing national Laboratory for Condensed Matter Physics and Institute of Physics Chinese Academy of SciencesBeijing 100190China Shanghai Key Laboratory of Material Frontiers Research in Extreme Environments(MFree) Shanghai Advanced Research in Physical Sciences(SHARPS)Shanghai 201203China

Following the recent report by Dasenbrock-Gammon et al.[nature 615,244–250(2023)]of near-ambient superconductivity in nitrogendoped lutetium trihydride(LuH_(3-δ)n_(ε)),significant debate has emerged surrounding the composition and interpretation of the observed sharp resistance ***,we meticulously revisit these claims through comprehensive characterization and *** definitively identify the reported material as lutetium dihydride(LuH_(2)),resolving the ambiguity surrounding its *** similar conditions(270–295 K and 1–2 GPa),we replicate the reported sharp decrease in electrical resistance with a 30%success rate,aligning with the observations by Dasenbrock-Gammon et ***,our extensive investigations reveal this phenomenon to be a novel pressure-induced metal-to-metal transition intrinsic to LuH_(2),distinct from ***,nitrogen doping exerts minimal impact on this *** work not only elucidates the fundamental properties of LuH_(2)andLuH_(3),but also critically challenges the notion of superconductivity in these lutetium hydride *** findings pave the way for future research on lutetium hydride systems,while emphasizing the crucial importance of rigorous verification in claims of ambient-temperature superconductivity.

关键词： resistance hydride superconductivity

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nMR Studies on Structural Features of DnA[d(TCGCGCG)]_2 With a Dangling Residue T

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Science China Chemistry 1994年第10期37卷 1177-1184页

作者：王可玉张友杰 philip n.BORER nMR & Data Processing Laboratory Syracuse UniversitySyracuseNY 13244-4100USA Department of ChemistryWesleyan UniversityMiddletownCT 06457USA The Center for Analysis & Testing Central China Normal UniversityWuchang 430070PRC nMR & Data Processing Laboratory Syracuse UniversitySyracuseNY 13244-4100 USA

The DnA heptamer [d(TCGCGCG)]_2,which has an alternating pyrimidine-purine se- quence and a dangling T residue at the 5′-end has been used as a model for studying conformational charac- teristics of DnA duplexes with double-single strand junctions that occur during replication and transcription. The proton resonances of the molecule have been assigned by nOESY and TOCSY/HOHAHA experiments. The nOE intensities at seven different mixing times were quantified for calculating distance constraints with the two-spin *** experiments have uncovered the interesting structural features of the *** sugar proton coupling constants were determined by the *** experiment for each residue of the duplex to examine the sugar conformations of the DnA *** structural features of the heptamer in solution have been *** overall structure of the DnA duplex is basically of a B-type DnA,and substantial changes from a standard B-DnA have been observed,especially in the terminal region of the *** dangling T residue was found in an unusual syn-conformation.

关键词： DAn conformation [d(TCGCGCG)]_2 2D nMR

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Pitting and stress corrosion cracking studies on AISI type 316n stainless steel weldments

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Defence Technology（防务技术） 2018年第3期14卷 226-237页

作者： Anita Toppo M.G.Pujar n.Sreevidya John philip Corrosion Science and Technology Division Indira Gandhi Centre for Atomic Research Materials Development and Technology Division Indira Gandhi Centre for Atomic Research

Pitting and stress corrosion cracking(SCC) studies on the 316 n stainless steel(SS) weldments in aswelded(AW) and thermally aged(solution annealing(SA), 550C/4 h and 750C/1 h) conditions were carried out in acidic and acidic chloride media. Pitting corrosion and SCC resistance was the highest for SA weldment, which was attributed to homogenization of microstructural and microchemical heterogeneities during solution annealing. SA specimen showed the most stable passive film as compared to AW weldment because of higher amount of delta-ferrite in AW weld metal, which resulted in an increased heterogeneity and inferior SCC resistance in AW. Stability of passive film was found to be adversely affected due to heat treatments(at 550C/4 h and 750C/1 h) because of the precipitation of carbide and sigma phases.

关键词：压力腐蚀不锈钢 AISI 焊接金属 SCC 稳定性氯化物微结构

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ERP Solution for Large & Medium State Firms

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Wuhan University Journal of natural Sciences 2001年第Z1期6卷 612-618页

作者： philip n.Zhang CEO of Wuhan Philwong Computer Technology Co.Ltd.

The application and development process of MRP-II /ERP can be divided into four stages as following. This period covers almost the entire 1980s', when the MRP-II was first introduced, implemented and partially applied to Chinese manufacturing SOEs (mostly machine tool manufacturing, automobile manufacturing). Because many obstacles are encountered and effect was limited, the period was also referred to "Three One-Third Theory" phase, that is one-third of foreign MRP-II software can be used, one-third needs to be modified before use and the rest one third is useless.

关键词： ERP Solution for Large Medium State Firms BPR

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