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Y_(1.95-x)Gd_xSiO_5:Eu_(0.05)的真空紫外光谱

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发光学报 2000年第2期21卷 145-149页

作者：宋桂兰尤洪鹏洪广言曾小青甘树才金昌弘卞钟洪庾炳容裴贤淑权一亿朴哲熙长春科学技术大学中国科学院长春应用化学研究所中国科学院长春应用化学研究所吉林长春130022 Division of Applied Scienxe

利用高温固相法合成了Ｙ１．９５－ｘＧｄｘＳｉＯ５：Ｅｕ０．０５（ｘ＝０．６ｍｏｌ％）荧光体。结构测定表明所合成的荧光体为单斜晶系的Ｘ２型Ｙ２ＳｉＯ５相，空间群为Ｂ２／ｂ。真空紫外光谱表明：随着Ｇｄ３＋含量的增加，在... 详细信息

利用高温固相法合成了Ｙ１．９５－ｘＧｄｘＳｉＯ５：Ｅｕ０．０５（ｘ＝０．６ｍｏｌ％）荧光体。结构测定表明所合成的荧光体为单斜晶系的Ｘ２型Ｙ２ＳｉＯ５相，空间群为Ｂ２／ｂ。真空紫外光谱表明：随着Ｇｄ３＋含量的增加，在１９２ｕｍ附近，出现了Ｇｄ＋的激发峰，且此峰的强度随着Ｇｄ３＋含量的增加而增大；同时位于１５０～１８５ｎｍ之间的基质吸收带的强度也增大；而位于２００～３００ｎｍ之间的Ｅｕ的电行迁移带的强度却随着Ｇｄ３＋含量的增加而降低。

关键词：真空紫外光谱基质吸收带荧光粉

Fungal diversity notes 491–602: taxonomic and phylogenetic contributions to fungal taxa

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Fungal Diversity 2017年第2期83卷 1-261页

作者： Saowaluck Tibpromma Kevin D.Hyde Rajesh Jeewon Sajeewa S.N.Maharachchikumbura Jian-Kui Liu D.Jayarama Bhat E.B.Gareth Jones Eric H.C.McKenzie Erio Camporesi Timur S.Bulgakov Mingkwan Doilom AndreLuiz Cabral Monteiro de Azevedo Santiago Kanad Das Patinjareveettil Manimohan Tatiana B.Gibertoni Young Woon Lim Anusha Hasini Ekanayaka Benjarong Thongbai Hyang Burm Lee Jun-Bo Yang Paul M.Kirk Phongeun Sysouphanthong Sanjay K.Singh Saranyaphat Boonmee Wei Dong K.N.Anil Raj K.P.Deepna Latha Rungtiwa Phookamsak Chayanard Phukhamsakda Sirinapa Konta Subashini C.Jayasiri Chada Norphanphoun Danushka S.Tennakoon Junfu Li Monika C.Dayarathne Rekhani H.Perera Yuanpin Xiao Dhanushka N.Wanasinghe Indunil C.Senanayake Ishani D.Goonasekara N.Ide Silva Ausana Mapook Ruvishika S.Jayawardena Asha J.Dissanayake Ishara S.Manawasinghe K.W.Thilini Chethana Zong-Long Luo Kalani Kanchana Hapuarachchi Abhishek Baghela Adriene Mayra Soares Alfredo Vizzini Angelina Meiras-Ottoni Armin Mesic Arun Kumar Dutta Carlos Alberto Fragoso de Souza Christian Richter Chuan-Gen Lin Debasis Chakrabarty Dinushani A.Daranagama Diogo Xavier Lima Dyutiparna Chakraborty Enrico Ercole Fang Wu Giampaolo Simonini Gianrico Vasquez Gladstone Alves da Silva Helio Longoni Plautz Jr Hiran A.Ariyawansa hyun Lee Ivana Kusan Jie song Jingzu Sun Joydeep Karmakar Kaifeng Hu Kamal C.Semwal Kasun M.Thambugala Kerstin Voigt Krishnendu Acharya Kunhiraman C.Rajeshkumar Leif Ryvarden Margita Jadan MdIqbal Hosen Michal Miksık Milan C.Samarakoon Nalin N.Wijayawardene Nam Kyu Kim Neven Matocec Paras Nath Singh Qing Tian R.P.Bhatt Rafael JoseVilela de Oliveira Rodham E.Tulloss S.Aamir Saithong Kaewchai Sayali D.Marathe Sehroon Khan Sinang Hongsanan Sinchan Adhikari Tahir Mehmood Tapas Kumar Bandyopadhyay Tatyana YuSvetasheva Thi Thuong Thuong Nguyen Vladimır Antonın Wen-Jing Li Yong Wang Yuvraj Indoliya Zdenko Tkalcec Abdallah M.Elgorban Ali H.Bahkali Alvin M.C.Tang Hong-Yan Su Huang Zhang Itthayakorn Promputtha Jennifer Luangsa-ard Jianchu Xu Jiye Yan Kang Ji-Chuan Marc Stadler Pete Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand World Agroforestry Centre East and Central AsiaKunming 650201YunnanPeople’s Republic of China Mushroom Research Foundation 128 M.3 Ban Pa Deng T.Pa PaeA.Mae TaengChiang Mai 50150Thailand A.M.B.Gruppo Micologico Forlivese“Antonio Cicognani” Via Roma 18Forlı`Italy A.M.B.Circolo Micologico“Giovanni Carini” C.P.314BresciaItaly Societa`per gli Studi Naturalistici della Romagna C.P.144BagnacavalloRAItaly Formerly Department of Botany Goa UniversityTaleigaoGoaIndia No.128/1-J Azad Housing SocietyCurcaGoa VelhaIndia 33 B St.Edwards Road Southsea Hants Hampshire PO53DHUK Landcare Research Manaaki Whenua Private Bag 92170AucklandNew Zealand Department of Biology Faculty of ScienceChiang Mai UniversityChiang Mai 50200Thailand Division of Applied Science College of International EducationThe Hong Kong Baptist UniversityHong Kong SARChina PostGraduate Program in Biology of Fungi Department of MycologyFederal University of PernambucoAv.Nelson Chavess/nRecifePE 50670-420Brazil Institute of Plant and Environment Protection Beijing Academy of Agriculture and Forestry SciencesBeijing 100097People’s Republic of China Environmental Microbiology Lab Division of Food TechnologyBiotechnology&AgrochemistryCollege of Agriculture and Life SciencesChonnam National UniversityYongbong-Dong 300Buk-GuGwangju 61186Korea Guizhou Key Laboratory of Agricultural Biotechnology Guizhou Academy of Agricultural SciencesGuiyang 550006GuizhouPeople’s Republic of China College of Agriculture and Biological Sciences Dali UniversityDali 671003YunnanPeople’s Republic of China Yunnan Institute of Food Safety Kunming University of Science&TechnologyKunming 650500YunnanPeople’s Republic

This is a continuity of a series of taxonomic and phylogenetic papers on the fungi where materials were collected from many countries,examined and *** addition to extensive morphological descriptions and appropriate asexual and sexual connections,DNA sequence data are also analysed from concatenated datasets to infer phylogenetic relationships and substantiate systematic positions of taxa within appropriate ***ever new species or combinations are proposed,we apply an integrative approach using morphological and molecular data as well as ecological features wherever *** on 112 fungal taxa are compiled in this paper including Biatriosporaceae and Roussoellaceae,Didysimulans ***.,81 new species,18 new host records and new country records,five reference specimens,two new combinations,and three sexual and asexual morph *** new species are Amanita cornelii,***,Angustimassarina alni,***,***,***,***,Ascochyta italica,***,Austroboletus appendiculatus,Barriopsis thailandica,Berkleasmium ariense,Calophoma petasitis,Camarosporium laburnicola,***,***,***,***,Colletotrichum sambucicola,Coprinopsis cerkezii,Cytospora gelida,Dacrymyces chiangraiensis,Didysimulans italica,***,Entodesmium italica,Entoloma magnum,Evlachovaea indica,Exophiala italica,Favolus gracilisporus,Femsjonia monospora,Fomitopsis flabellata,***,Gongronella brasiliensis,Helvella crispoides,Hermatomyces chiangmaiensis,***nae,Hysterium centramurum,Inflatispora caryotae,Inocybe brunneosquamulosa,***,***,Keissleriella cirsii,Lepiota cylindrocystidia,***,***,Lophiotrema guttulata,Marasmius luculentus,Morenoina calamicola,Moelleriella thanathonensis,Mucor stercorarius,Myrmecridium fluviae,Myrothecium septentrionale,Neosetophoma garethjonesii,Nigrograna cangshanensis,Nodulosphaeria guttulatum,***tata,***,Panus subf

关键词： Agaricomycetes Ascomycota Basidiomycota Dacrymycetes Dothideomycetes Eurotiomycetes New combination Mucoromycotina New genus New records New species Pezizomycetes Phylogeny Sordariomycetes Taxonomy

乙型肝炎病毒PreS_2S基因在毕赤酵母中表达的研究

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微生物学杂志 2006年第5期26卷 44-48页

作者：李利金立杰杨屹邵丽军谷丽娟刘岩松董厉子王宇长春生物制品研究所吉林长春130062

构建重组质粒phil-D2/PreS2S以研究乙肝病毒PreS2(120-146)S基因编码蛋白在毕赤酵母中的表达。通过PCR扩增获得PreS2S片段,插入含AOX1启动子的Pichia Pastoris表达载体phil-D2中,构建重组表达质粒phil-D2/PreS2S,转化酵母宿主菌GS115。挑取阳性克隆摇床培养,甲醇诱导表达。通过ELISA、RPHA鉴定表达产物。成功构建了phil-D2/PreS2S真核表达载体,经过序列分析,插入的基因为在中国流行的adr亚型。在毕赤酵母中重组载体表达了S蛋白,S蛋白的表达量为34.9 mg/L,PreS2抗原检测为强阳性。利用毕赤酵母表达系统能够有效地表达乙型肝炎病毒的PreS2S蛋白,PreS2S蛋白具有良好的生物学活性。

关键词： PreS2基因 S基因毕赤酵母表达

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转甲状腺素蛋白基因在酵母中的表达

生物工程学报 1999年第4期15卷 428-433页

作者：程颖高卜渝宋大新袁汉英陈永青李育阳柳湘田培坤顾健人复旦大学微生物系复旦大学遗传学研究所遗传工程国家重点实验室上海市肿瘤研究所上海200031

用限制酶ＢａｍＨＩ将含人ＴＴＲ基因的ＤＮＡ片段（约４９３ｂｐ）从质粒ｐＳＫ ＴＴＲ上切下后并插入到分泌型载体ｐＨＩＬ ＳＩ的ＢａｍＨＩ位点上，经酶切鉴定重组质粒ｐＨＩＬ ＳＩ... 详细信息

用限制酶ＢａｍＨＩ将含人ＴＴＲ基因的ＤＮＡ片段（约４９３ｂｐ）从质粒ｐＳＫ ＴＴＲ上切下后并插入到分泌型载体ｐＨＩＬ ＳＩ的ＢａｍＨＩ位点上，经酶切鉴定重组质粒ｐＨＩＬ ＳＩ ＴＴＲ上的ＴＴＲ基因插入方向正确。将ｐＨＩＬ ＳＩ ＴＴＲＤＮＡ用ＢｇｌＩＩ酶切后，转入真核表达系统———毕赤氏酵母。得到的转化子经摇瓶发酵，上清液用１５％ＳＤＳ ＰＡＧＥ检测，有ＴＴＲＦ的表达。经ＤＥＡＥ ＳｅｐｈｅｒｏｓｅＦ．Ｆ离子交换柱和ＳｅｐｈａｃｒｙｌＳ２００分子筛层析，得到纯化的ＴＴＲＦ。体外实验表明ＴＴＲＦ

关键词：转甲状腺素蛋白分泌型载体毕赤氏酵母肝癌细胞