检索结果-南通市图书馆

Cortisol levels and serum antioxidant status following chemotherapy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Novel and Functional Artificial Bruch’s Membrane Made of Poly (ε-Caprolactone)/Gelatin for Retinal Pigment Epithelium Restoration

Health（健康） 2011年第8期

作者： Eugenia Limberaki phaedra eleftheriou Georgios Gasparis Eugenios Karalekos Vassilis Kostoglou Christos Petrou

来源：评论

学校读者我要写书评

暂无评论

Advances in Bioscience and Biotechnology 2024年第8期15卷 443-461页

作者： López Edith Silva-Bermudez phaedra Gonzalez-Salinas Roberto Velasquillo Cristina Torres-Romero Abigail López-Colomé Ana María Pérez Armando Gina Prado-Prone Edgar Ramos-Martínez Iván Ramos-Martínez Quiroz-Mercado Hugo Hernández-Zimbrón Luis Fernando Instituto de Fisiologa Celular Universidad Nacional Autnoma de Mxico Ciudad de Mxico City Mxico Unidad de Ingeniería de Tejidos Terapia Celular y Medicina Regenerativa Instituto Nacional de Rehabilitación Luis Guiller-mo Ibarra Ibarra Ciudad de México México Departamento de Investigacin Asociacin Para Evitar la Ceguera Ciudad de Mxico Mxico Subdireccin de Investigacin Tecnolgica Instituto Nacional de Rehabilitacin Luis Guillermo Ibarra Ibarra Ciudad de Mxico Mxico Departamento de Biologa Celular y Tisular Facultad de Medicina Universidad Nacional Autnoma de Mxico Ciudad de Mxico Mxico Escuela de Ciencias Universidad Autnoma Benito Jurez de Oaxaca Oaxaca Mxico Departamento de Medicina y Zootecnia de Cerdos Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional utnoma de Mxico Mxico Laboratorio de Investigacion Interdisciplinaria rea de Optometria Escuela Nacional de Estudios Superiores Unidad Len Universidad Nacional Autnoma de Mxico Len Guanajuato Mxico Laboratorio Nacional de ptica de la Visin-Centro de Investigaciones en ptica Len Guanajuato Mxico

Purpose: The primary aim of this paper is to design, create, and improve functional and artificial Bruch’s membranes (BM) using bioengineering techniques, which can be applied in the treatment of maculopathies by supporting the growth and maintenance of retinal pigment epithelium (RPE) cells, thereby potentially enabling subretinal implantation in patients. Methods: We fabricated by electrospinning ultrathin 3D nanofibrous membranes from Polycaprolactone (PCL), and different concentrations of gelatin (5%, 15% and 30%). ARPE-19 cells were seeded onto these artificial membranes. MTT assays were performed in order to evaluate ARPE-19 cell viability and cytotoxicity. IF assays were realized to observe the ARPE-19 cells onto each membrane. Ultrastructure of the modified Bruch’s membrane and ARPE-19 morphology after 25 days of culture were studied with transmission and scanning electron microscopy. To evaluate expression changes in markers of ARPE-19 (RPE65 and ZO-1) qRT-PCR assays were realized. Data from three independent experiments were pooled and expressed as the mean SD. A confidence level of P 0.05 was considered to be statistically significant. Results ARPE-19 cells grew on PCL/Gelatin membranes mainly in PCL/30% gelatin combination, which had not cytotoxic effect. RPE65/cytokeratin-18/ and actin-positive ARPE-19 cells formed a correctly orientated monolayer of polygonal cells with morphological polarity. The apical cell surfaces exhibited abundant protruding microfolds. Moreover, zones of polygonal border look as if ARPE-19 cells fused among, suggesting the presence of tight junctions. The expression of RPE65 and ZO-1 was unchanged. Conclusions: PCL/30% Gelatine membranes may imitate the natural BM to such extent that they support RPE-cells and exhibited RPE-like morphology. The engineering of a human RPE monolayer with these artificial BM, emulating the in vivo retina, arouse their potential subretinal implantation in patients with wet age-related macular degene

关键词： Artificial Bruch’s Membrane Electrospinning ARPE-19 Cells Age-Related Macular Degeneration

Biocompatibility and electrochemical evaluation of ZrO_(2) thin films deposited by reactive magnetron sputtering on MgZnCa alloy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Magnesium and Alloys 2021年第6期9卷 2019-2038页

作者： Benjamín Millán-Ramos Daniela Morquecho-Marín phaedra Silva-Bermudez David Ramírez-Ortega Osmary Depablos-Rivera Julieta García-López Mariana Fernández-Lizárraga JoséVictoria-Hernández Dietmar Letzig Argelia Almaguer-Flores Sandra E.Rodil Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de MéxicoCDMX 04510Mexico Posgrado en Ciencia e Ingeniería de Materiales Universidad Nacional Autónoma de MéxicoCDMX 04510Mexico Unidad de Ingeniería de Tejidos Terapia Celular y Medicina RegenerativaInstituto Nacional de Rehabilitación Luis Guillermo IbarraCDMX 14389Mexico Posgrado en Ciencias Médicas Odontológicas y de la SaludUniversidad Nacional Autónoma de MéxicoCDMX 04510Mexico Instituto de Ciencias Aplicadas y Tecnologia Universidad Nacional Autónoma de MéxicoCDMX 04510Mexico Posgrado de Doctorado en Ciencias en Biomedicina y Biotecnología Molecular Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalCDMX 11340Mexico Institute of Material and Process Design Helmholtz-Zentrum GeesthachtMax-Planck-Strasse 1Geesthacht 21502Germany Magnesium Innovation Centre MagIC Helmholtz-Zentrum GeesthachtMax-Planck-Strasse 1Geesthacht 21502Germany Laboratorio de Biointerfases Facultad de OdontologíaDivisión de Estudios de Posgrado e InvestigaciónUniversidad Nacional Autónoma de MéxicoCDMX 04510Mexico

Biodegradable magnesium alloys are promising candidates for temporary fracture fixation devices in orthopedics;nevertheless,its fast degradation rate at the initial stage after implantation remains as one of the main challenges to be ***_(2)-based coatings to reduce the degradation rate of the Mg-implants are an attractive solution since they show high biocompatibility and *** this work,the degradation,cytotoxicity,and antibacterial performance of ZrO_(2)thin films deposited by magnetron sputtering on a Mg-Zn-Ca alloy was ***-term degradation of ZrO_(2)-coated and uncoated samples was assessed considering electrochemical techniques and H_(2)evolution(gas chromatography).Additionally,long term degradation was assessed by mass-loss *** results showed that a 380 nm ZrO_(2)coating reduces the degradation rate and H_(2)evolution of the alloy during the initial 3 days after immersion but allows the degradation of the bare alloy for the *** ZrO_(2)coating does not compromise the biocompatibility of the alloy and permits better cell adhesion and proliferation of mesenchymal stem cells directly on its surface,in comparison to the bare ***,the ZrO_(2)coating prevents the adhesion and biofilm formation of ***.

关键词： Zirconium dioxide Magnetron sputtering Short-term degradation H_(2)evolution

Health Impact of Pesticides on Residents and Horticultural Workers in the Lake Naivasha Region, Kenya

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Occupational Diseases and Environmental Medicine 2015年第2期3卷 24-34页

作者： Pamela F. Tsimbiri Wilkister N. Moturi Judith Sawe phaedra Henley John R. Bend Department of Reproductive Health Faculty of Health Sciences Egerton University Njoro Nakuru Kenya Department of Environmental Science Faculty of Environment and Natural Resources Development Egerton University Egerton Kenya Department of Community and Public Health Faculty of Health Sciences Egerton University Njoro Nakuru Kenya Ecosystem Health Research Program Department of Pathology Schulich School of Medicine and Dentistry Western University London ON Canada Schulich Inter Faculty Program in Public Health The Western Centre for Public Health and Family Medicine Western University London ON Canada

The horticultural sector has undergone tremendous growth in Kenya and is now the second largest foreign income earning sector in the country. Lake Naivasha is the hub of large scale horticultural farming in Kenya. This growth coupled with increased use of pesticides, may increase the possibility of pesticide exposure and its associated risks to workers and residents of the region. The purpose of this study was to assess the symptoms commonly experienced by residents of Naivasha town and their possible association to pesticide exposure. Purposive sampling of residents from Kamere, Kwa Muia, Kioto and Karagita was performed, as these residential areas have significant numbers of flower farm workers. By random selection, a total of 801 community members were recruited to participate in the study and data was collected by completing the prepared questionnaire and from clinical examinations. Results indicate that several residents exhibited respiratory;skin, joints and bones;and nervous system symptoms. We found a higher frequency of symptoms among planters, weeders, harvesters than in sprayers working in horticultural farms. We recommend training to planters, weeders, harvesters who are mainly women (61.6%) in this study concerning pesticide use as do sprayers;and second, longer reentry times between the last spraying of pesticide and entry of these workers is warranted, particularly in greenhouses. In this regard, reentry times for greenhouses and fields established for specific pesticides in Europe, North America, Japan or Australia should be adapted by Kenya, and these guidelines enforced by the Government of Kenya to reduce exposure to pesticides within this vulnerable work group. These workers should also use protective clothing including gloves and masks at all times while handling chemicals or recently sprayed plants or flowers. It would also be prudent for flower farm owners to introduce an integrated pest management regime to reduce pesticide use andworker expo

关键词： Pesticides Health Effects Horticultural Workers

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

一个创业者妻子的告白

品质 2006年第6期 64-72页

作者： phaedra Hise 青子（编译）

她支持她的丈夫去创办一家大企业．她为能支持丈夫实现梦想而自豪。但5年的时间太漫长了．她看着丈夫心力交瘁而又束手无策5年的时间里．她几乎失去了自己的丈夫和其他的一切。

关键词：创业者妻子丈夫时间管理企业发展