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The Establishment of an Agrobacterium-Mediated Transformation Platform for the Non-Embryogenic Calli of Vitis vinifera L.

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Agricultural Sciences in China 2011年第5期10卷 686-694页

作者： ZHAO Feng-xia CHEN Shang-wu perl avihai DAI Ru XU Hai-ying MA Hui-qin College of Food Science and Nutrition Engineering China Agricultural University Beijing 100063 P.R. China Department of Fruit Tree Sciences Volcani Center Bet Dagan 50250 Israel College of Agriculture and Biotechnology China Agricultural University Beijing 100193 P.R.China Institute of Forestry and Fruit Tree Sciences Beijing Academy of Agricultural Sciences Beijing 100093 P.R.China

Non-embryogenic calli （NEC） was inevitably and heavily produced when grape embryogenic calli （EC） was induced from explants or during the subculture of EC.A stable and highly efficient NEC transformation platform is required to further sort out and verify key genes which determine/switch the identity of NEC and *** this research,a vector pA5 containing a chitinase signal sequence fused to gfp （green fluorescent protein） and an HDEL motive was used to target and immobilize into Agrobacterium strain EHA105 to establish a transformation platform for Vitis vinifera *** *** was determined that NEC 10 d after subculture was the best target tissue;30 min for inoculation followed by 3 d co-cultivation with the addition of 200 μmol L-1 acetosyringone （AS） was optimized as *** use of bacterial densities as 1.0 at OD600 did not result in serious tissue hypersensitive reaction and it had higher *** at 200 mg L-1 was picked for positive expression *** stable transformation of NEC was proved by reverse transcription-polymerase chain reaction techniques （RT-PCR） and fluorescent microscopy after three sub-cultures of the selected cell *** efficient genetic transformation protocol of grape NEC was achieved and some of the optimized parameters were different from that reported for *** transformation platform could facilitate the verification of candidate somatic embryogenesis （SE） decisive genes,and the successfully transformed NEC with certain genes can also be used as bioreactors for the production of functional products,as NEC not only proliferates fast,but also keeps in a rather stable condition.

关键词： Agrobacterium tumefaciens non-embryogenic calli transformation Vitis vinifera L. RT-PCR

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Cloning and Characterization of WOX4 Gene from Vitis vinifera L. Involved in Stem Cell Regulation

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Agricultural Sciences in China 2011年第12期10卷 1861-1871页

作者： DAI Ru JIN Hai-peng WANG Zeng avihai perl XU Hai-ying ZHANG Wen CHEN Shang-wu MA Hui-qin College of Agriculture and Biotechnology China Agricultural University Beijing 100193 P.R. China Department of Fruit Tree Breeding and Molecular Genetics Agricultural Research Organization Bet-Dagan 50250 Israel Institute of Forestry and Fruit Tree Sciences Beijing Academy of Agricultural Sciences Beijing 100093 P.R. China Key Lab of Functional Dairy Science Ministry of Education of China Municipal Government of Beijing/College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 P.R. China

Wuschel-related homeobox （WOX） genes play essential, specific, and sometimes redundant roles in plant embryo development, shoot and root meristem maintenance, and plant development. Though much information was quickly gained with members of the WOX gene family of Arabidopsis, monocotyledonous crops, and gymnospermous conifers, little is known about perennial woody plants. In this study, we isolated the first WOXgene family member from grape （Vitis vinifera L. cv. Cabernet Sauvignon）, and named it VvWOX4 based on its characteristic domains and phylogenetic analysis. The identity of VvWOX4 was validated by MALDI-TOF MS and Western blot with polyclonal antibody against Arabidopsis thaliana Wuschel. Functional analysis showed that VvWOX4 markedly increased shoot primordia structures when overexpressed under CaMV 35S promoter in tobacco. A different expression pattern was found for VvVOX4 compared with AtWUCHEL and its expression was detected in unique organs of grapevines. Besides the expression in the vegetative shoot apical meristem （SAM） of grape shoot tips, VvWOX4 is expressed in dormant winter buds, inflorescence, young leaves, and tendril tips, but not in root tips. In young leaves, the expression of VvWOX4 is strongly upregulated by wounding, and also by plant growth regulators such as 2 mg L-1 2,4-D, 1 mg L-1 NAA and 1 mg L-1 BAP treatments, while downregulation was monitored by 1 mg L^-1 IBA treatment, and there was no response to 0.5 mg L-1 GA3 treatment. Together, our results revealed the first member of grape WOX gene family and indicated different roles and regulation of VvWOX4 in the perennial woody crop grapevine.

关键词： expression characteristics gene cloning plant hormone induced expression Htis vinifera L. WOX4

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The mTOR pathway in the antiphospholipid syndrome

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Medical Review 2023年第6期3卷 511-513页

作者： Lanlan Ji Zhuoli Zhang Andras perl Department of Rheumatology and Clinical Immunology Peking University First HospitalBeijingChina Department of Rheumatology and Clinical Immunology Peking University First HospitalNo.8Xishiku StreetBeijing 100034China Departments of Medicine Microbiology and ImmunologyBiochemistry and Molecular BiologyDivision of Rheumatology and Clinical ImmunologyState University of New YorkUMUCollege of Medicine750 East Adams StreetSyracuseNY 13210USA

This perspective discussed the available evidence on the involvement of mTOR pathway in antiphospholipid syndrome(APS),from the aspects of endothelial cells,platelets,monocytes and anti-phospholipid antibodies(PLs),wh... 详细信息

关键词： antiphospholipid syndrome mTOR autoimmune disease

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Explainability-based Trust Algorithm for electricity price forecasting models

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Energy and AI 2023年第4期14卷 141-158页

作者： Leena Heistrene Ram Machlev Michael perl Juri Belikov Dmitry Baimel Kfir Levy Shie Mannor Yoash Levron Electrical Engineering Department School of TechnologyPandit Deendayal Energy UniversityGandhinagarGujarat382007India The Andrew and Erna Viterbi Faculty of Electrical&Computer Engineering Technion-Israel Institute of TechnologyHaifa3200003Israel Department of Software Science Tallinn University of TechnologyAkadeemia tee 15ATallinn12618Estonia Electrical&Electronics Engineering Department Sami Shamoon College of EngineeringBeer-Sheva84100Israel

Advanced machine learning(ML)algorithms have outperformed traditional approaches in various forecasting applications,especially electricity price forecasting(EPF).However,the prediction accuracy of ML reduces substantially if the input data is not similar to the ones seen by the model during *** is often observed in EPF problems when market dynamics change owing to a rise in fuel prices,an increase in renewable penetration,a change in operational policies,*** the dip in model accuracy for unseen data is a cause for concern,what is more,challenging is not knowing when the ML model would respond in such a *** uncertainty makes the power market participants,like bidding agents and retailers,vulnerable to substantial financial loss caused by the prediction errors of EPF ***,it becomes essential to identify whether or not the model prediction at a given instance is *** this light,this paper proposes a trust algorithm for EPF users based on explainable artificial intelligence *** suggested algorithm generates trust scores that reflect the model’s prediction quality for each new *** scores are formulated in two stages:in the first stage,the coarse version of the score is formed using correlations of local and global explanations,and in the second stage,the score is fine-tuned further by the Shapley additive explanations values of different *** score-based explanations are more straightforward than feature-based visual explanations for EPF users like asset managers and traders.A dataset from Italy’s and ERCOT’s electricity market validates the efficacy of the proposed *** show that the algorithm has more than 85%accuracy in identifying good predictions when the data distribution is similar to the training *** the case of distribution shift,the algorithm shows the same accuracy level in identifying bad predictions.

关键词： Electricity price forecasting EPF Explainable AI model XAI SHAP Explainability

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The detrimental effect of autofrettage on externally cracked modern tank gun barrels

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Defence Technology（防务技术） 2019年第2期15卷 146-153页

作者： M.perl T.Saley Pearlstone Center for Aeronautical Engineering Studies Department of Mechanical Engineering Ben-Gurion University of the Negev

Overstraining gun tubes has a twofold advantage. First, it enables the increase of the Safe Maximum Pressure(SMP) in the tube, resulting in a higher muzzle velocity which extends the gun's range and its projectile kinetic energy. Second, it reduces the tube's susceptibility to internal cracking which prolongs its fatigue life. Unfortunately, autofrettage also bears an inherent detrimental effect as it considerably increases the tensile hoop stress at the outer portion of the barrel's wall, which enhances external cracking of the tube by increasing the prevailing Stress Intensity Factor(SIF). In order to quantify this disadvantageous effect, 3-D Mode I SIFs distributions along the front of a single external radial semielliptical crack initiating from the outer surface of an autofrettaged modern gun barrel, overstrained by either the Swage or the Hydraulic autofrettage processes, are evaluated. The analysis is performed by the finite element(FE) method, using singular elements along the crack front. Innovative residual stress fields(RSFs), incorporating the Bauschinger effect for both types of autofrettage are applied to the ***'s [1] RSF is also applied to the tube for comparison reasons. All three RSFs are incorporated in the FE analysis, using equivalent temperature fields, Values for K_(IA)-the SIF resulting from the tensile residual stresses induced by autofrettage are evaluated for: a typical barrel of radii ratio R_o/R_i = 2, crack depth to wall-thickness ratios(a/t = 0.005-0.1),crack ellipticities(a/c = 0.2-1.0),and five levels of Swage,Hydraulic and Hill's autofrettage(e = 40%,60%,70%,80%,and 100%). In total,375 different 3-D cases are analyzed. The analysis demonstrates undoubtedly the detrimental effect of all types of autofrettage in increasing the prevailing effective stress intensity factor of external cracks, resulting in crack initiation enhancement and crack growth rate acceleration which considerably shortens the total fatigue life of the bar

关键词： Gun barrel Swage and hydraulic autofrettage External crack 3-D Finite element

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Explainable Artificial Intelligence (XAI) techniques for energy and powersystems: Review, challenges and opportunities

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能源与人工智能(英文) 2022年第3期9卷 193-205页

作者： R.Machlev L.Heistrene M.perl K.Y.Levy J.Belikov S.Mannor Y.Levron The Andrew and Erna Viterbi Faculty of Electrical&Computer Engineering Technion—Israel Institute of TechnologyHaifa 3200003Israel Department of Software Science Tallinn University of TechnologyAkadeemia tee 15a12618 TallinnEstonia School of Technology Pandit Deendayal Energy UniversityGandhinagar 382007GujaratIndia

Despite widespread adoption and outstanding performance, machine learning models are considered as ‘‘blackboxes’’, since it is very difficult to understand how such models operate in practice. Therefore, in the powersystems field, which requires a high level of accountability, it is hard for experts to trust and justify decisionsand recommendations made by these models. Meanwhile, in the last couple of years, Explainable ArtificialIntelligence (XAI) techniques have been developed to improve the explainability of machine learning models,such that their output can be better understood. In this light, it is the purpose of this paper to highlight thepotential of using XAI for power system applications. We first present the common challenges of using XAI insuch applications and then review and analyze the recent works on this topic, and the on-going trends in theresearch community. We hope that this paper will trigger fruitful discussions and encourage further researchon this important emerging topic.

关键词： Power Energy Neural network Deep-learning Explainable artificial intelligence XAI

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Hippo/Yap signaling controls epithelial progenitor cell proliferation and differentiation in the embryonic and adult lung

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分子细胞生物学报:英文版 2015年第1期7卷 35-47页

作者： Alexander W.Lange Anusha Sridharan Yan Xu Barry R.Stripp Anne-Karina perl Jeffrey A.Whitsett Division of Pulmonary Biology Cindinnati Children's Hospital Medical Center and the University of Cncinnati Collel ge of MedicineCincinnatiOH 452293039USA Cedars-Sinai Medical Center Los AngelesCA 90048USA

The Hippo/Yap pathwayis a wll-conserved signaling cascade that regulates cell proliferation and dfferentiationto control organsize and stem/progenitor cell *** airway injury,Yap was dynamically regulated in regenerating airway epithelial *** *** the role ofHippo signaling in the lung,the mammalian Hippo kinases,Mst1 and Mst2,were deleted in epithelial cells ofthe embry-onic and mature mouse ***1/2 deletion in the fetal lung enhanced proliferation and inhibited sacculation and epithelial cell *** transcriptional inhibition of cell proliferation and activation of differentiation during normal perinatal lung maturation were inversely regulated followving embryonic Mst1/2 *** of Mst1/2 from bronchiolar epithelial cells in the adult lung caused airway hyperplasia and altered *** Yap phosphorylation was decreased and Yap nuclear localization and transcriptional targets were increased after Mst1/2 deletion,consistent with canonical Hippo/Yap *** potentiated cell prolif-eration and inhibited dfferentiation of human bronchial epithelial cells in *** of Mst1/2 and expression ofYAP regulated transcrip-tional targets controlling cell proliferation and diferentiation,including Ajuba LIM *** was required forthe effects ofYAP oncell proliferation in ***/Yapsignaling regulates Ajuba and controls proliferation and diferentiation oflung epithelial progenitor cells.

关键词： Hippo/Yap pathway lung Ajuba proliferation dfferetiation

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Flexible Defense Succeeds Creative Attacks!—A Simulation Approach Based on Position Data in Professional Football

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Journal of Software Engineering and Applications 2021年第9期14卷 493-504页

作者： Daniel Memmert Jonas Imkamp Jürgen perl German University of Sport Science Cologne Germany University of Mainz Mainz Germany

Introduction: The key to success is finding the perfect mixture of tactical patterns and sudden breaks of them, which depends on the behavior of the opponent team and is not easy to estimate by just watching matches. According to the specific tactical team behavior of “attack vs. defense” professional football matches are investigated based on a simulation approach, professional football matches are investigated according to the specific tactical team behavior of “attack vs. defense.” Methods: The formation patterns of all the sample games are categorized by SOCCER© for defense and attack. Monte Carlo-Simulation can evaluate the mathematical, optimal strategy. The interaction simulation between attack and defense shows optimal flexibility rates for both tactical groups. Approach: A simulation approach based on 40 position data sets of the 2014/15 German Bundesliga has been conducted to analyze and optimize such strategic team behavior in professional soccer. Results: The results revealed that both attack and defense have optimal planning rates to be more successful. The more complex the success indicator, the more successful attacking player groups get. The results also show that defensive player groups always succeed in attacking groups below a specific planning rate value. Conclusion: Groups are always succeeding. The simulation-based position data analysis shows successful strategic behavior patterns for attack and defense. Attacking player groups need very high flexibility to be successful (stay in ball possession). In contrast, defensive player groups only need to be below a defined flexibility rate to be guaranteed more success.

关键词： Strategy KPI Offensive and Defensive Dynamics Ball Contact Ball Possession Passes

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欢迎您到荷兰来?!?

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英语学习 2003年第1期 28-29页

作者： Emily perl Kingsley 邢彩霞内蒙古工业大学外国语学院大英教研室

常有人要我写点关于养育残疾儿童的经验,来帮助那些从没体会过这一独特经历的人们理解它,去想像那是一种什么样的感觉。它就像这样·,,…在你的孩子还未出世之前,整件事就像计划一次完美的度假旅行-去意大利。你买来一大螺的导游书,作... 详细信息

常有人要我写点关于养育残疾儿童的经验,来帮助那些从没体会过这一独特经历的人们理解它,去想像那是一种什么样的感觉。它就像这样·,,…在你的孩子还未出世之前,整件事就像计划一次完美的度假旅行-去意大利。你买来一大螺的导游书,作了精彩的安

关键词：威尼斯意大利

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2008北京第五届全科医学与社区卫生服务高级论坛内容摘登

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中国全科医学 2009年第8期12卷 623-624页

作者：张大发武琳 David perl North 本刊编辑部北京市劳动和社会保障局北京市西城区月坛街道木樨地社区卫生服务站美国Wis Consin家庭医学院

北京市医疗保险与社区慢性病管理工作的开展张大发教授:大家下午好,非常高兴能够参加今天的高峰论坛会。医疗保险和医疗机构的关系是非常密切的。下面我就如何既保证广大人民群众的身体健康,同时又能够合理地使用医疗费和大家进行探讨... 详细信息

北京市医疗保险与社区慢性病管理工作的开展张大发教授:大家下午好,非常高兴能够参加今天的高峰论坛会。医疗保险和医疗机构的关系是非常密切的。下面我就如何既保证广大人民群众的身体健康,同时又能够合理地使用医疗费和大家进行探讨。北京市医疗保险的基本情况及面临的问题北京市在医疗保险的制度上率先实现了人人享有医疗保险的奋斗目标,现已覆盖1600万北京市城镇居民。但与此同时也面临着以下问题:(1)持续上涨的医疗费用给医疗保险管理带来了巨大的压力,尤其是门诊医疗费用支出持续几年来增长幅度都超过20%,门诊医疗费用增长的幅度超过了职工工资的增长水平;(2)医疗保险面临着庞大的快速增长的慢性病人群。据北京市卫生局公布的2005年北京市成人慢性病相关危险因素相关报告显示,高血压的患病率为29·1%,但是控制率仅为11·7%,糖尿病的患病率为7·5%,控制率仅为15·9%,血脂异常的患病率为38·0%,控制率仅为11·4%。我们对医疗保险基金支出情况进行了分析,结果表明用于上述疾病及其并发症住院的医疗费用占医疗保险住院总费用的31%,所以有效控制这三种疾病及其并发症是控制报销医疗基金快速增长的有效途径。北京市医疗保险管理问题的解决方...

关键词：全科医学社区卫生服务卫生服务管理

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